{"id":"https://openalex.org/W1657028362","doi":"https://doi.org/10.4049/jimmunol.138.9.2953","title":"cDNA cloning and sequencing reveals that the electrophoretically constant DR beta 2 molecules, as well as the variable DR beta 1 molecules, from HLA-DR2 subtypes have different amino acid sequences including a hypervariable region for a functionally important epitope.","display_name":"cDNA cloning and sequencing reveals that the electrophoretically constant DR beta 2 molecules, as well as the variable DR beta 1 molecules, from HLA-DR2 subtypes have different amino acid sequences including a hypervariable region for a functionally important epitope.","publication_year":1987,"publication_date":"1987-05-01","ids":{"openalex":"https://openalex.org/W1657028362","doi":"https://doi.org/10.4049/jimmunol.138.9.2953","mag":"1657028362","pmid":"https://pubmed.ncbi.nlm.nih.gov/3571980"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.138.9.2953","doi":"https://doi.org/10.4049/jimmunol.138.9.2953","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.138.9.2953","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021450326","display_name":"Shih-Kwang Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S K Wu","raw_affiliation_strings":["Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":null,"display_name":"T Yabe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T Yabe","raw_affiliation_strings":["Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042805731","display_name":"Michael Madden","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M Madden","raw_affiliation_strings":["Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000492384","display_name":"Thomas L. Saunders","orcid":"https://orcid.org/0000-0003-2015-101X","observed_orcids":["https://orcid.org/0000-0003-2015-101X"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T L Saunders","raw_affiliation_strings":["Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113629670","display_name":"Fritz H. Bach","orcid":"https://orcid.org/0009-0000-3953-3363","observed_orcids":["https://orcid.org/0009-0000-3953-3363"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F H Bach","raw_affiliation_strings":["Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunobiology Research Center, Departments of Laboratory Medicine, Pathology, and Surgery, University of Minnesota , Minneapolis, MN 55455","institution_ids":["https://openalex.org/I130238516"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I130238516"],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":2.6168,"has_fulltext":false,"cited_by_count":35,"citation_normalized_percentile":{"value":0.90382835,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"138","issue":"9","first_page":"2953","last_page":"2959"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12576","display_name":"vaccines and immunoinformatics approaches","score":0.8324999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12576","display_name":"vaccines and immunoinformatics approaches","score":0.8324999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10261","display_name":"Genetic Associations and Epidemiology","score":0.09570000320672989,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.009399999864399433,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hla-dr2","display_name":"HLA-DR2","score":0.9869999885559082},{"id":"https://openalex.org/keywords/hla-class-ii-polymorphism","display_name":"HLA class II polymorphism","score":0.6949999928474426},{"id":"https://openalex.org/keywords/hypervariable-region","display_name":"hypervariable region","score":0.9070000052452087},{"id":"https://openalex.org/keywords/amino-acid-variation","display_name":"amino acid sequence variation","score":0.675000011920929},{"id":"https://openalex.org/keywords/epitopes","display_name":"antigenic determinants","score":0.6010000109672546},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.6100000143051147},{"id":"https://openalex.org/keywords/electrophoretic-mobility","display_name":"electrophoretic mobility","score":0.5529999732971191},{"id":"https://openalex.org/keywords/hla-dw2","display_name":"HLA-Dw2","score":0.824999988079071}],"concepts":[{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.805259644985199},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.6676055788993835},{"id":"https://openalex.org/C2776174256","wikidata":"https://www.wikidata.org/wiki/Q830842","display_name":"BETA (programming language)","level":2,"score":0.6204147934913635},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5947622656822205},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.5720068216323853},{"id":"https://openalex.org/C81566733","wikidata":"https://www.wikidata.org/wiki/Q1047544","display_name":"Hypervariable region","level":3,"score":0.5585163235664368},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5499691963195801},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5488177537918091},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.428916335105896},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.31889039278030396},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006681","descriptor_name":"HLA-D Antigens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006684","descriptor_name":"HLA-DR Antigens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007526","descriptor_name":"Isoelectric Point","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.4049/jimmunol.138.9.2953","doi":"https://doi.org/10.4049/jimmunol.138.9.2953","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.138.9.2953","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},{"id":"pmid:3571980","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3571980","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunology (Baltimore, Md. : 1950)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1149614517","display_name":null,"funder_award_id":"AI17687","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G1336139182","display_name":null,"funder_award_id":"AI19007","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G3236199076","display_name":null,"funder_award_id":"AI18326","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2026389007","https://openalex.org/W2020509768","https://openalex.org/W2164337482","https://openalex.org/W2207584063","https://openalex.org/W1801736477","https://openalex.org/W1989625049","https://openalex.org/W1992993527","https://openalex.org/W2001596283","https://openalex.org/W2073556262","https://openalex.org/W2024209282"],"abstract_inverted_index":{"Two-dimensional":[0],"gel":[1],"electrophoresis":[2],"(2D-PAGE)":[3],"of":[4,16,36,91,129,140,151,223],"DR":[5,25,40,49,86,121,144,162,175,189,199],"molecules":[6,42,123,146,165],"from":[7,68,125,135,203],"three":[8],"different":[9,209],"Dw":[10],"subtypes":[11],"(Dw2,":[12],"Dw12,":[13,205],"and":[14,52,70,79,84,97,101,112,142,157,166,172,206],"FJO)":[15,83],"the":[17,37,53,102,120,130,136,148,152,167,186,196,219,224],"HLA-DR2":[18],"haplotype":[19],"reveals":[20],"that":[21,34,185],"at":[22],"least":[23],"two":[24,38,114,153,169],"beta":[26,41,50,56,87,122,145,163,176,190,200],"genes":[27],"are":[28],"expressed.":[29],"Protein":[30],"mixing":[31],"experiments":[32],"demonstrate":[33],"one":[35],"expressed":[39],"is":[43],"electrophoretically":[44,197],"variable":[45,198],"(referred":[46],"to":[47],"as":[48,159,193,195],"1),":[51],"other":[54,111,143,168],"(DR":[55],"2)":[57],"migrates":[58],"constantly":[59,187],"among":[60],"DR2":[61],"subtypes.":[62],"We":[63],"have":[64,208],"constructed":[65],"cDNA":[66,88,155,170],"libraries":[67],"Dw12":[69,78],"FJO":[71,81,207],"homozygous":[72],"typing":[73],"cells":[74],"(HTC":[75],"DHO":[76],"for":[77,82,119,161,174],"HTC":[80],"isolated":[85],"clones.":[89],"Four":[90],"these":[92,141],"clones":[93],"(FJO-13,":[94],"DHO-8,":[95],"FJO-6,":[96],"DHO-7)":[98],"were":[99,107],"sequenced,":[100],"deduced":[103],"amino":[104,116,137,211],"acid":[105,117,138,180,212],"sequences":[106,118,139],"compared":[108],"with":[109,113],"each":[110],"published":[115],"derived":[124],"a":[126,215],"DR2-Dw2HTC.":[127],"Prediction":[128],"migration":[131],"patterns":[132],"on":[133],"2D-PAGE":[134],"allows":[147],"tentative":[149],"designation":[150],"full-length":[154],"(DHO-8":[156],"FJO-13)":[158],"coding":[160],"2":[164,191],"(DHO-7":[171],"FJO-6)":[173],"1":[177,201],"molecules.":[178],"Amino":[179],"sequence":[181],"comparisons":[182],"also":[183],"show":[184],"migrating":[188],"molecules,":[192,202],"well":[194],"Dw2,":[204],"primary":[210],"sequences,":[213],"including":[214],"clustered":[216],"difference":[217],"in":[218],"third":[220],"hypervariable":[221],"region":[222],"polymorphic":[225],"first":[226],"domain.":[227]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}