{"id":"https://openalex.org/W165036232","doi":"https://doi.org/10.1007/978-1-4939-0733-5_2","title":"Minimum Information Necessary for Quantitative Real-Time PCR Experiments","display_name":"Minimum Information Necessary for Quantitative Real-Time PCR Experiments","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W165036232","doi":"https://doi.org/10.1007/978-1-4939-0733-5_2","mag":"165036232","pmid":"https://pubmed.ncbi.nlm.nih.gov/24740217"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-4939-0733-5_2","doi":"https://doi.org/10.1007/978-1-4939-0733-5_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4939-0733-5_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2488100277","display_name":"Quantitative Real-Time PCR"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://figshare.com/articles/chapter/Minimum_Information_Necessary_for_Quantitative_Real-Time_PCR_Experiments/23774262","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032323495","display_name":"Gemma L. Johnson","orcid":"https://orcid.org/0000-0002-2826-7925","observed_orcids":["https://orcid.org/0000-0002-2826-7925","https://orcid.org/0000-0001-7101-6876"]},"institutions":[{"id":"https://openalex.org/I166337079","display_name":"Queen Mary University of London","ror":"https://ror.org/026zzn846","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I166337079"]},{"id":"https://openalex.org/I4405263501","display_name":"Blizard Institute","ror":"https://ror.org/01y7pzx08","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I166337079","https://openalex.org/I4405263501"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Gemma Johnson","raw_affiliation_strings":["Blizard Institute of Cellular and Molecular Science, Queen Mary University, London, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Blizard Institute of Cellular and Molecular Science, Queen Mary University, London, UK","institution_ids":["https://openalex.org/I166337079","https://openalex.org/I4405263501"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080752621","display_name":"Afif M. Abdel Nour","orcid":"https://orcid.org/0000-0002-4491-7692","observed_orcids":["https://orcid.org/0000-0002-4491-7692"]},"institutions":[{"id":"https://openalex.org/I185163786","display_name":"King Abdulaziz University","ror":"https://ror.org/02ma4wv74","country_code":"SA","type":"education","lineage":["https://openalex.org/I185163786"]}],"countries":["SA"],"is_corresponding":false,"raw_author_name":"Afif Abdel Nour","raw_affiliation_strings":["Special Infectious Agents Unit-Biosafety Level 3, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Special Infectious Agents Unit-Biosafety Level 3, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia","institution_ids":["https://openalex.org/I185163786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110679900","display_name":"Tania Nolan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Tania Nolan","raw_affiliation_strings":["Sigma-Aldrich, Haverhill, Suffolk, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sigma-Aldrich, Haverhill, Suffolk, UK","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052898193","display_name":"Jim F. Huggett","orcid":"https://orcid.org/0000-0002-0945-1911","observed_orcids":["https://orcid.org/0000-0002-0945-1911"]},"institutions":[{"id":"https://openalex.org/I190229588","display_name":"LGC","ror":"https://ror.org/00fbx7096","country_code":"GB","type":"company","lineage":["https://openalex.org/I190229588"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jim Huggett","raw_affiliation_strings":["Molecular and Cell Biology, LGC Ltd, Queens Road, Teddington, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular and Cell Biology, LGC Ltd, Queens Road, Teddington, UK","institution_ids":["https://openalex.org/I190229588"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043596447","display_name":"Stephen Andrew Bustin","orcid":"https://orcid.org/0000-0003-1870-6098","observed_orcids":["https://orcid.org/0000-0003-1870-6098"]},"institutions":[{"id":"https://openalex.org/I51216347","display_name":"Anglia Ruskin University","ror":"https://ror.org/0009t4v78","country_code":"GB","type":"education","lineage":["https://openalex.org/I51216347"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Stephen Bustin","raw_affiliation_strings":["Postgraduate Medical Institute, Faculty of Health, Social Care and Education, Anglia Ruskin University, Bishop Hall Lane, Chelmsford, Essex, CM1 1SQ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Postgraduate Medical Institute, Faculty of Health, Social Care and Education, Anglia Ruskin University, Bishop Hall Lane, Chelmsford, Essex, CM1 1SQ, UK","institution_ids":["https://openalex.org/I51216347"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5043596447"],"corresponding_institution_ids":["https://openalex.org/I51216347"],"apc_list":null,"apc_paid":null,"fwci":11.9427,"has_fulltext":false,"cited_by_count":90,"citation_normalized_percentile":{"value":0.98982284,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":99},"biblio":{"volume":"1160","issue":null,"first_page":"5","last_page":"17"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10206","display_name":"Meta-analysis and systematic reviews","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1804","display_name":"Statistics, Probability and Uncertainty"},"field":{"id":"https://openalex.org/fields/18","display_name":"Decision Sciences"},"domain":{"id":"https://openalex.org/domains/2","display_name":"Social Sciences"}},{"id":"https://openalex.org/T12648","display_name":"Academic Writing and Publishing","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/3309","display_name":"Library and Information Sciences"},"field":{"id":"https://openalex.org/fields/33","display_name":"Social Sciences"},"domain":{"id":"https://openalex.org/domains/2","display_name":"Social Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/miqe-guidelines","display_name":"MIQE guidelines","score":0.9929999709129333},{"id":"https://openalex.org/keywords/quantitative-real-time-pcr","display_name":"quantitative real-time PCR","score":0.8539999723434448},{"id":"https://openalex.org/keywords/reporting-guidelines","display_name":"reporting guidelines","score":0.4909999966621399},{"id":"https://openalex.org/keywords/assay-design","display_name":"assay design","score":0.7699999809265137},{"id":"https://openalex.org/keywords/data-transparency","display_name":"data transparency","score":0.36899998784065247},{"id":"https://openalex.org/keywords/reproducibility","display_name":"reproducibility","score":0.6819999814033508},{"id":"https://openalex.org/keywords/research-integrity","display_name":"research integrity","score":0.32199999690055847}],"concepts":[{"id":"https://openalex.org/C155911762","wikidata":"https://www.wikidata.org/wiki/Q422321","display_name":"Blueprint","level":2,"score":0.7176631093025208},{"id":"https://openalex.org/C2778223634","wikidata":"https://www.wikidata.org/wiki/Q224952","display_name":"Suspect","level":2,"score":0.7081045508384705},{"id":"https://openalex.org/C2780233690","wikidata":"https://www.wikidata.org/wiki/Q535347","display_name":"Transparency (behavior)","level":2,"score":0.667680025100708},{"id":"https://openalex.org/C2522767166","wikidata":"https://www.wikidata.org/wiki/Q2374463","display_name":"Data science","level":1,"score":0.5221336483955383},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4928781986236572},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.4478057622909546},{"id":"https://openalex.org/C17744445","wikidata":"https://www.wikidata.org/wiki/Q36442","display_name":"Political science","level":0,"score":0.2695009112358093},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.24580827355384827},{"id":"https://openalex.org/C38652104","wikidata":"https://www.wikidata.org/wiki/Q3510521","display_name":"Computer security","level":1,"score":0.17004844546318054},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.1525806486606598},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.1190744936466217},{"id":"https://openalex.org/C199539241","wikidata":"https://www.wikidata.org/wiki/Q7748","display_name":"Law","level":1,"score":0.10903024673461914},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.09046432375907898},{"id":"https://openalex.org/C78519656","wikidata":"https://www.wikidata.org/wiki/Q101333","display_name":"Mechanical engineering","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D017408","descriptor_name":"Guidelines as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1007/978-1-4939-0733-5_2","doi":"https://doi.org/10.1007/978-1-4939-0733-5_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4939-0733-5_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:24740217","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24740217","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.637.1156","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.637.1156","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"http://www.gene-quantification.com/johnson-et-al-miqe-mimb-ch2-2014.pdf","raw_type":"text"},{"id":"pmh:oai:arro.anglia.ac.uk:334957","doi":null,"is_oa":false,"landing_page_url":"https://arro.anglia.ac.uk/id/eprint/334957/","pdf_url":null,"source":{"id":"https://openalex.org/S4306402145","display_name":"Anglia Ruskin Research Online (Anglia Ruskin University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I51216347","host_organization_name":"Anglia Ruskin University","host_organization_lineage":["https://openalex.org/I51216347"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Book Chapter"},{"id":"pmh:oai:figshare.com:article/23774262","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/chapter/Minimum_Information_Necessary_for_Quantitative_Real-Time_PCR_Experiments/23774262","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Chapter"}],"best_oa_location":{"id":"pmh:oai:figshare.com:article/23774262","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/chapter/Minimum_Information_Necessary_for_Quantitative_Real-Time_PCR_Experiments/23774262","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Chapter"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1834270560","https://openalex.org/W1980574031","https://openalex.org/W2010296175","https://openalex.org/W2018856693","https://openalex.org/W2019331497","https://openalex.org/W2027351830","https://openalex.org/W2029069236","https://openalex.org/W2049162919","https://openalex.org/W2057464020","https://openalex.org/W2060287830","https://openalex.org/W2099066165","https://openalex.org/W2106962167","https://openalex.org/W2107726313","https://openalex.org/W2117483311","https://openalex.org/W2127535576","https://openalex.org/W2140535402","https://openalex.org/W2143146123","https://openalex.org/W2148818032","https://openalex.org/W2151601111","https://openalex.org/W2157308941","https://openalex.org/W2164095252","https://openalex.org/W2165151268","https://openalex.org/W2168420558","https://openalex.org/W4237866497","https://openalex.org/W4241470000"],"related_works":["https://openalex.org/W2148818032","https://openalex.org/W1971314022","https://openalex.org/W2168420558","https://openalex.org/W7098615080","https://openalex.org/W7097786508","https://openalex.org/W7096544235","https://openalex.org/W4391302727","https://openalex.org/W109333173","https://openalex.org/W2049162919","https://openalex.org/W2106962167"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":10},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":12},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":9},{"year":2014,"cited_by_count":6}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}