{"id":"https://openalex.org/W16367453","doi":null,"title":"Insight into O-GlcNAc protein modification using chemical and biochemical tools","display_name":"Insight into O-GlcNAc protein modification using chemical and biochemical tools","publication_year":2010,"publication_date":"2010-05-10","ids":{"openalex":"https://openalex.org/W16367453","mag":"16367453"},"language":"en","primary_location":{"id":"pmh:oai:summit.sfu.ca:node-12146","doi":null,"is_oa":false,"landing_page_url":"https://summit.sfu.ca/_flysystem/fedora/sfu_migrate/12146/etd6025_MMacauley.pdf","pdf_url":"https://summit.sfu.ca/_flysystem/fedora/sfu_migrate/12146/etd6025_MMacauley.pdf","source":{"id":"https://openalex.org/S4306400490","display_name":"Summit (Simon Fraser University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I18014758","host_organization_name":"Simon Fraser University","host_organization_lineage":["https://openalex.org/I18014758"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087354201","display_name":"Matthew Scott Macauley","orcid":"https://orcid.org/0000-0003-4579-1048","observed_orcids":["https://orcid.org/0000-0003-4579-1048"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Matthew S. Macauley","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4579-1048","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5087354201"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00131381,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/o-glcnacylation","display_name":"O-GlcNAcylation","score":0.9909999966621399},{"id":"https://openalex.org/keywords/o-glcnacase","display_name":"O-GlcNAcase","score":0.7979999780654907},{"id":"https://openalex.org/keywords/o-glcnac-transferase","display_name":"O-GlcNAc transferase","score":0.9359999895095825},{"id":"https://openalex.org/keywords/selective-enzyme-inhibitors","display_name":"selective enzyme inhibitors","score":0.7390000224113464},{"id":"https://openalex.org/keywords/off-target-effects","display_name":"off-target effects","score":0.8880000114440918},{"id":"https://openalex.org/keywords/substrate-assisted-catalysis","display_name":"substrate-assisted catalysis","score":0.9210000038146973},{"id":"https://openalex.org/keywords/insulin-resistance","display_name":"insulin resistance","score":0.7730000019073486},{"id":"https://openalex.org/keywords/insulin-signaling","display_name":"insulin signaling","score":0.9150000214576721},{"id":"https://openalex.org/keywords/protein-phosphorylation","display_name":"protein phosphorylation","score":0.6679999828338623},{"id":"https://openalex.org/keywords/molecular-chaperones","display_name":"molecular chaperones","score":0.8740000128746033}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4527086615562439},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4343085289001465},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43369874358177185},{"id":"https://openalex.org/C100631289","wikidata":"https://www.wikidata.org/wiki/Q898362","display_name":"Posttranslational modification","level":3,"score":0.41367143392562866},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.36120742559432983},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28394949436187744},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14263257384300232}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:summit.sfu.ca:node-12146","doi":null,"is_oa":false,"landing_page_url":"https://summit.sfu.ca/_flysystem/fedora/sfu_migrate/12146/etd6025_MMacauley.pdf","pdf_url":"https://summit.sfu.ca/_flysystem/fedora/sfu_migrate/12146/etd6025_MMacauley.pdf","source":{"id":"https://openalex.org/S4306400490","display_name":"Summit (Simon Fraser University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I18014758","host_organization_name":"Simon Fraser University","host_organization_lineage":["https://openalex.org/I18014758"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"pmh:oai:summit.sfu.ca:12146","doi":null,"is_oa":false,"landing_page_url":"http://summit.sfu.ca/item/12146","pdf_url":null,"source":{"id":"https://openalex.org/S4306400490","display_name":"Summit (Simon Fraser University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I18014758","host_organization_name":"Simon Fraser University","host_organization_lineage":["https://openalex.org/I18014758"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:16367453","doi":null,"is_oa":false,"landing_page_url":"https://summit.sfu.ca/item/12146","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2093721490","https://openalex.org/W2056711756","https://openalex.org/W1999804990","https://openalex.org/W2022647011","https://openalex.org/W12350796","https://openalex.org/W2263939622","https://openalex.org/W246126671","https://openalex.org/W7098234982","https://openalex.org/W7096987564","https://openalex.org/W3127884649"],"abstract_inverted_index":{"Within":[0],"higher":[1],"eukaryotes,":[2],"hundreds":[3],"of":[4,25,33,41,60,88,105,122,146,155,161,169,197,202,252,272,291,344],"nucleocytoplasmic":[5],"proteins":[6,43,116],"are":[7,83,256,280],"modified":[8],"by":[9,22,318],"an":[10,66,144,152,204],"N-acetylglucosamine":[11],"(GlcNAc)":[12],"residue.":[13],"This":[14],"O-GlcNAc":[15,28,62,69,81,113,148,235,300,319,346],"modification":[16,63,201],"is":[17,64,108,211,261],"dynamic,":[18],"a":[19,45,163,167,177,186,194,257,294,305,316],"property":[20],"imparted":[21,314],"the":[23,31,51,57,61,93,109,159,285,289,311,341,345],"actions":[24],"two":[26,118,125],"enzymes:":[27],"transferase":[29],"catalyzes":[30],"installation":[32],"GlcNAc":[34],"onto":[35],"specific":[36],"serine":[37],"and":[38,70,97,132,265,328],"threonine":[39],"residues":[40],"target":[42],"via":[44],"\uf062-glycosidic":[46],"linkage,":[47],"while":[48],"O-GlcNAcase":[49,165,175,214],"removes":[50],"modification.":[52,347],"A":[53,101],"leading":[54,286],"hypothesis":[55,287,296,332],"for":[56,213,288],"cellular":[58,75],"role(s)":[59],"that":[65,174,210,249,262],"interplay":[67],"between":[68],"phosphorylation":[71,87],"exists":[72],"to":[73,85,103,111,192,233,282,315],"fine-tune":[74],"signaling":[76,90,95],"pathways.":[77],"In":[78],"particular,":[79],"elevated":[80],"levels":[82,114,149],"thought":[84],"prevent":[86],"key":[89,102],"molecules":[91],"in":[92,229],"insulin":[94,99,245],"cascade":[96],"cause":[98,244],"resistance.":[100],"many":[104],"these":[106,141,156,184],"studies":[107,171],"ability":[110],"modulate":[112],"on":[115],"using":[117,140],"previously":[119,253],"described":[120],"inhibitors":[121,255],"O-GlcNAcase.":[123,198],"These":[124,278,324],"inhibitors,":[126],"however,":[127],"also":[128],"inhibit":[129],"other":[130],"targets":[131],"so":[133],"it":[134],"was":[135,190,208,297],"unclear":[136],"if":[137],"results":[138,279],"obtained":[139],"compounds":[142],"were":[143],"effect":[145,154],"modulating":[147],"or,":[150],"alternatively,":[151],"off-target":[153,250],"inhibitors.":[157],"With":[158],"goal":[160],"developing":[162],"selective":[164,212],"inhibitor,":[166],"series":[168],"biochemical":[170],"provided":[172],"evidence":[173],"uses":[176],"catalytic":[178],"mechanism":[179],"involving":[180],"substrate-assisted":[181],"catalysis.":[182],"Through":[183],"studies,":[185],"compound":[187],"called":[188],"NAG-thiazoline":[189],"found":[191],"be":[193,302],"potent":[195],"inhibitor":[196,205,243],"By":[199],"chemical":[200,326],"NAG-thiazoline,":[203],"termed":[206],"NButGT":[207,222,239,273],"generated":[209],"over":[215],"functionally-related":[216],"enzymes":[217],"yet":[218],"retains":[219],"high":[220,270],"potency.":[221],"functions":[223],"within":[224,231],"cells":[225,264],"as":[226,228,304],"well":[227],"vivo":[230],"rodents":[232],"elevate":[234],"levels.":[236],"Surprisingly,":[237],"neither":[238],"nor":[240],"another":[241],"new":[242,295,322,325],"resistance,":[246],"strongly":[247],"suggesting":[248],"effects":[251],"used":[254],"concern.":[258],"More":[259],"surprising":[260],"cultured":[263],"animals":[266],"treated":[267],"long-term":[268],"with":[269,284],"doses":[271],"show":[274],"no":[275],"adverse":[276],"effects.":[277],"challenging":[281],"reconcile":[283],"role":[290,343],"O-GlcNAc.":[292],"Therefore,":[293],"formed":[298,331],"whereby":[299],"may":[301],"acting":[303],"protein":[306,317],"chaperone.":[307],"Preliminary":[308],"experiments":[309],"examining":[310],"biophysical":[312],"properties":[313],"support":[320],"this":[321,329],"hypothesis.":[323],"tools":[327],"newly":[330],"should":[333],"enable":[334],"exciting":[335],"future":[336],"research":[337],"aimed":[338],"at":[339],"understanding":[340],"functional":[342]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}