{"id":"https://openalex.org/W163208399","doi":"https://doi.org/10.1042/bj1090161","title":"Biosynthesis of thiamine. Incorporation experiments with 14C-labelled substrates and with [15N]glycine in Saccharomyces cerevisiae","display_name":"Biosynthesis of thiamine. Incorporation experiments with 14C-labelled substrates and with [15N]glycine in Saccharomyces cerevisiae","publication_year":1968,"publication_date":"1968-09-01","ids":{"openalex":"https://openalex.org/W163208399","doi":"https://doi.org/10.1042/bj1090161","mag":"163208399","pmid":"https://pubmed.ncbi.nlm.nih.gov/5679359","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1186770"},"language":"en","primary_location":{"id":"doi:10.1042/bj1090161","doi":"https://doi.org/10.1042/bj1090161","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj1090161","pdf_url":"https://portlandpress.com/biochemj/article-pdf/109/2/161/761545/bj1090161.pdf","source":{"id":"https://openalex.org/S4210234214","display_name":"Biochemical Journal","issn_l":"0306-3283","issn":["0306-3283"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://portlandpress.com/biochemj/article-pdf/109/2/161/761545/bj1090161.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113457538","display_name":"P E Linnett","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405285715","display_name":"National Institute for Medical Research","ror":"https://ror.org/00146rb91","country_code":"GB","type":"facility","lineage":["https://openalex.org/I2801081054","https://openalex.org/I4210087105","https://openalex.org/I4405285715","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"P. E. Linnett","raw_affiliation_strings":["National Institute for Medical Research, Mill Hill, London, N.W. 7"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute for Medical Research, Mill Hill, London, N.W. 7","institution_ids":["https://openalex.org/I4405285715"]}]},{"author_position":"last","author":{"id":null,"display_name":"J. Walker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405285715","display_name":"National Institute for Medical Research","ror":"https://ror.org/00146rb91","country_code":"GB","type":"facility","lineage":["https://openalex.org/I2801081054","https://openalex.org/I4210087105","https://openalex.org/I4405285715","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"J. Walker","raw_affiliation_strings":["National Institute for Medical Research, Mill Hill, London, N.W. 7"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute for Medical Research, Mill Hill, London, N.W. 7","institution_ids":["https://openalex.org/I4405285715"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4405285715"],"apc_list":null,"apc_paid":null,"fwci":0.8063,"has_fulltext":true,"cited_by_count":27,"citation_normalized_percentile":{"value":0.61768448,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"109","issue":"2","first_page":"161","last_page":"168"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12915","display_name":"Biochemical Acid Research Studies","score":0.9115999937057495,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12915","display_name":"Biochemical Acid Research Studies","score":0.9115999937057495,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12494","display_name":"Alcoholism and Thiamine Deficiency","score":0.04859999939799309,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.0066999997943639755,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thiamine-biosynthesis","display_name":"thiamine biosynthesis","score":1.0},{"id":"https://openalex.org/keywords/14c-labeled-substrates","display_name":"14C-labeled substrates","score":0.7070000171661377},{"id":"https://openalex.org/keywords/15n-glycine","display_name":"15N-glycine","score":0.8859999775886536},{"id":"https://openalex.org/keywords/nitrogen-incorporation","display_name":"nitrogen incorporation","score":0.8349999785423279},{"id":"https://openalex.org/keywords/methionine","display_name":"methionine","score":0.6669999957084656},{"id":"https://openalex.org/keywords/serine","display_name":"serine","score":0.7250000238418579},{"id":"https://openalex.org/keywords/formate","display_name":"formate","score":0.8019999861717224},{"id":"https://openalex.org/keywords/acetate","display_name":"acetate","score":0.984000027179718},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8180000185966492},{"id":"https://openalex.org/keywords/pyrimidine-biosynthesis","display_name":"pyrimidine synthesis","score":0.5590000152587891},{"id":"https://openalex.org/keywords/thiazole-biosynthesis","display_name":"thiazole biosynthesis","score":0.9470000267028809}],"concepts":[{"id":"https://openalex.org/C2778464347","wikidata":"https://www.wikidata.org/wiki/Q413426","display_name":"Thiazole","level":2,"score":0.8868700265884399},{"id":"https://openalex.org/C2777756961","wikidata":"https://www.wikidata.org/wiki/Q620730","display_name":"Glycine","level":3,"score":0.8352652788162231},{"id":"https://openalex.org/C2779321679","wikidata":"https://www.wikidata.org/wiki/Q207722","display_name":"Pyrimidine","level":2,"score":0.7289486527442932},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.657012939453125},{"id":"https://openalex.org/C2776203871","wikidata":"https://www.wikidata.org/wiki/Q83187","display_name":"Thiamine","level":2,"score":0.6394975185394287},{"id":"https://openalex.org/C2780178953","wikidata":"https://www.wikidata.org/wiki/Q2001841","display_name":"Formate","level":3,"score":0.6384285688400269},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6031892895698547},{"id":"https://openalex.org/C2776568683","wikidata":"https://www.wikidata.org/wiki/Q23005640","display_name":"Moiety","level":2,"score":0.5919393301010132},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5225157737731934},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.48591652512550354},{"id":"https://openalex.org/C2780378348","wikidata":"https://www.wikidata.org/wiki/Q25351438","display_name":"Ring (chemistry)","level":2,"score":0.4586053788661957},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.38199126720428467},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3549080491065979},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.22371789813041687},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1040380597114563},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.07752805948257446}],"mesh":[{"descriptor_ui":"D000085","descriptor_name":"Acetates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002247","descriptor_name":"Carbon Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005561","descriptor_name":"Formates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005998","descriptor_name":"Glycine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008715","descriptor_name":"Methionine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009584","descriptor_name":"Nitrogen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011743","descriptor_name":"Pyrimidines","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011868","descriptor_name":"Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012440","descriptor_name":"Saccharomyces","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012694","descriptor_name":"Serine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013831","descriptor_name":"Thiamine","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013831","descriptor_name":"Thiamine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D013844","descriptor_name":"Thiazoles","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1042/bj1090161","doi":"https://doi.org/10.1042/bj1090161","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj1090161","pdf_url":"https://portlandpress.com/biochemj/article-pdf/109/2/161/761545/bj1090161.pdf","source":{"id":"https://openalex.org/S4210234214","display_name":"Biochemical Journal","issn_l":"0306-3283","issn":["0306-3283"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1186770","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1186770","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmid:5679359","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/5679359","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1042/bj1090161","doi":"https://doi.org/10.1042/bj1090161","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj1090161","pdf_url":"https://portlandpress.com/biochemj/article-pdf/109/2/161/761545/bj1090161.pdf","source":{"id":"https://openalex.org/S4210234214","display_name":"Biochemical Journal","issn_l":"0306-3283","issn":["0306-3283"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W163208399.pdf","grobid_xml":"https://content.openalex.org/works/W163208399.grobid-xml"},"referenced_works_count":28,"referenced_works":["https://openalex.org/W20017895","https://openalex.org/W975118258","https://openalex.org/W1512603168","https://openalex.org/W1548072470","https://openalex.org/W1816812487","https://openalex.org/W1825071869","https://openalex.org/W1968423308","https://openalex.org/W1976600932","https://openalex.org/W1984181934","https://openalex.org/W2009502139","https://openalex.org/W2012369748","https://openalex.org/W2014631153","https://openalex.org/W2038887384","https://openalex.org/W2040374135","https://openalex.org/W2044829204","https://openalex.org/W2060308855","https://openalex.org/W2075380858","https://openalex.org/W2142666127","https://openalex.org/W2300225935","https://openalex.org/W2321153361","https://openalex.org/W2341011193","https://openalex.org/W2401062856","https://openalex.org/W2403535582","https://openalex.org/W2408380671","https://openalex.org/W2414019989","https://openalex.org/W2414056485","https://openalex.org/W2415208285","https://openalex.org/W2428070221"],"related_works":["https://openalex.org/W2300225935","https://openalex.org/W1522582794","https://openalex.org/W2159371227","https://openalex.org/W2043782229","https://openalex.org/W202055246","https://openalex.org/W2002810194","https://openalex.org/W213576463","https://openalex.org/W2052712522","https://openalex.org/W2069559100","https://openalex.org/W2028889260"],"abstract_inverted_index":{"1.":[0],"Yeast":[1],"was":[2,24,81,85,114],"grown":[3],"in":[4],"a":[5,11],"minimal":[6],"synthetic":[7],"medium":[8],"together":[9],"with":[10],"range":[12],"of":[13,43,67,93,100,123,135],"(14)C-labelled":[14,75],"substrates":[15],"under":[16],"standardized":[17],"conditions.":[18],"After":[19],"isolation,":[20],"the":[21,29,54,64,70,94,105,111,124,132,138],"purified":[22,35],"thiamine":[23],"cleaved":[25],"by":[26],"sulphite":[27],"and":[28,31,36,49,56,61,79,121,131],"pyrimidine":[30,112],"thiazole":[32,95,106,125],"moieties":[33],"were":[34],"assayed":[37],"for":[38,53,63],"radioactivity.":[39],"2.":[40],"In":[41],"order":[42],"decreasing":[44],"incorporation,":[45],"[(14)C]formate,":[46],"[3-(14)C]serine,":[47,58],"[2-(14)C]glycine":[48],"[2-(14)C]acetate":[50,62],"supplied":[51],"label":[52,68,90],"pyrimidine,":[55],"[2-(14)C]glycine,":[57],"[1-(14)C]glycine,":[59],"[(14)C]formate":[60],"thiazole.":[65],"Incorporation":[66],"into":[69,104,110],"fragments":[71],"from":[72,102,129],"several":[73],"other":[74],"substrates,":[76],"including":[77],"[Me-(14)C]-":[78],"[3,4-(14)C(2)]-methionine,":[80],"insignificant.":[82],"3.":[83],"[3-(14)C]Serine":[84],"shown":[86],"not":[87,109,143],"to":[88,91,145],"contribute":[89],"C-2":[92,120,130],"ring.":[96],"4.":[97],"Significant":[98],"incorporation":[99],"nitrogen":[101,133],"[(15)N]glycine":[103],"moiety,":[107,113],"but":[108,137],"established.":[115],"5.":[116],"It":[117],"appears":[118],"that":[119],"N-3":[122],"ring":[126],"are":[127],"formed":[128],"atom":[134],"glycine,":[136],"entire":[139],"methionine":[140],"molecule":[141],"does":[142],"appear":[144],"be":[146],"implicated.":[147]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}