{"id":"https://openalex.org/W16305166","doi":null,"title":"Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.","display_name":"Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.","publication_year":1988,"publication_date":"1988-12-01","ids":{"openalex":"https://openalex.org/W16305166","mag":"16305166","pmid":"https://pubmed.ncbi.nlm.nih.gov/2462139"},"language":"en","primary_location":{"id":"pmid:2462139","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2462139","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Leukemia","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111390863","display_name":"DR Sutherland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801845744","display_name":"Toronto General Hospital","ror":"https://ror.org/026pg9j08","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I1325899441","https://openalex.org/I2801845744"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"D R Sutherland","raw_affiliation_strings":["Oncology Clinic, Toronto General Hospital, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Oncology Clinic, Toronto General Hospital, Ontario, Canada","institution_ids":["https://openalex.org/I2801845744"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057299611","display_name":"Suzanne M. Watt","orcid":"https://orcid.org/0000-0001-7684-8022","observed_orcids":["https://orcid.org/0000-0001-7684-8022"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S M Watt","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026071388","display_name":"G Dowden","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G Dowden","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108240281","display_name":"Kimmo K. Karhi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Karhi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111388784","display_name":"M. A. Baker","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M A Baker","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111607029","display_name":"Melvyn F. Greaves","orcid":"https://orcid.org/0000-0002-7239-1384","observed_orcids":["https://orcid.org/0000-0002-7239-1384"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M F Greaves","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111427889","display_name":"J. E. Smart","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J E Smart","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.1149,"has_fulltext":false,"cited_by_count":75,"citation_normalized_percentile":{"value":0.75718547,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"2","issue":"12","first_page":"793","last_page":"803"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9750999808311462,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9750999808311462,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.004800000227987766,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cd34","display_name":"CD34","score":0.8399999737739563},{"id":"https://openalex.org/keywords/hematopoietic-progenitor-cells","display_name":"hematopoietic progenitor cells","score":0.8970000147819519},{"id":"https://openalex.org/keywords/cell-surface-antigens","display_name":"cell surface antigens","score":0.8899999856948853},{"id":"https://openalex.org/keywords/glycoprotein-structure","display_name":"glycoprotein structure","score":0.8539999723434448},{"id":"https://openalex.org/keywords/n-glycans","display_name":"N-glycans","score":0.9409999847412109},{"id":"https://openalex.org/keywords/glycosylation","display_name":"glycosylation","score":0.7620000243186951},{"id":"https://openalex.org/keywords/tunicamycin","display_name":"tunicamycin","score":0.7910000085830688},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.8140000104904175},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.7360000014305115}],"concepts":[{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.6473729014396667},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6231092810630798},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5813239812850952},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5589738488197327},{"id":"https://openalex.org/C2780654058","wikidata":"https://www.wikidata.org/wiki/Q5376339","display_name":"Endoglycosidase","level":3,"score":0.47774308919906616},{"id":"https://openalex.org/C206212055","wikidata":"https://www.wikidata.org/wiki/Q2553138","display_name":"Glycan","level":3,"score":0.41952696442604065},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.39451664686203003},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34935063123703003},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.32728731632232666},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.08674192428588867}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000943","descriptor_name":"Antigens, Differentiation","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D000943","descriptor_name":"Antigens, Differentiation","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D000951","descriptor_name":"Antigens, Neoplasm","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009363","descriptor_name":"Neoplasm Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018952","descriptor_name":"Antigens, CD34","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:2462139","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2462139","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Leukemia","raw_type":"Journal Article"},{"id":"mag:16305166","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2462139","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1214205619","https://openalex.org/W2015046018","https://openalex.org/W1935828301","https://openalex.org/W2071993158","https://openalex.org/W2034909662","https://openalex.org/W2410853501","https://openalex.org/W1507601091","https://openalex.org/W2033617962","https://openalex.org/W7096082989","https://openalex.org/W2036846953"],"abstract_inverted_index":{"Monoclonal":[0],"antibodies":[1,104],"of":[2,13,74,113,120,175,193],"the":[3,55,114,118,176,180],"CD34":[4,103,139,196],"class":[5],"all":[6],"recognize":[7],"a":[8,173],"monomeric":[9],"cell":[10],"surface":[11],"antigen":[12,56,140,170,197],"approximately":[14],"Mr":[15],"110,000":[16],"which":[17],"is":[18,57,141,171],"selectively":[19],"expressed":[20],"on":[21],"human":[22],"hemopoietic":[23],"progenitor":[24],"cells.":[25],"This":[26],"structure":[27,131],"can":[28],"be":[29],"readily":[30],"surface-labeled":[31],"with":[32,42,123,156,203],"[125I]actoperoxidase":[33],"and":[34,70,163],"by":[35,61,77,145],"periodate-[3H]borohydride,":[36],"but":[37],"it":[38,154],"labels":[39],"only":[40],"weakly":[41],"[35S]methionine,":[43],"[35Sl]cysteine,":[44],"3H-amino":[45],"acids,":[46],"or":[47],"3H-mannose,":[48],"even":[49],"after":[50],"prolonged":[51],"labeling":[52,125],"periods.":[53],"However,":[54],"more":[58],"efficiently":[59,107],"labeled":[60,144],"[3H]glucosamine.":[62],"Lectin":[63],"binding":[64],"studies,":[65,126],"sensitivity":[66],"to":[67,106],"certain":[68],"glycosidases,":[69],"gel":[71],"filtration":[72],"analysis":[73,192],"glycans":[75,88],"released":[76],"alkaline":[78],"hydrolysis":[79],"indicate":[80,167],"that":[81,101,129,168,182],"this":[82,130,169],"glycoprotein":[83],"contains":[84],"several":[85,92,186],"complex-type":[86],"N-linked":[87],"as":[89,91],"well":[90],"highly":[93,194],"sialylated":[94],"O-linked":[95],"glycans.":[96],"Western":[97,164],"blotting":[98,165],"experiments":[99],"show":[100],"various":[102],"fail":[105],"detect":[108],"desialylated":[109],"and/or":[110],"de-N-glycosylated":[111],"forms":[112],"antigen.":[115],"Experiments":[116],"involving":[117],"use":[119],"tunicamycin,":[121],"together":[122],"metabolic":[124],"strongly":[127],"suggest":[128],"\"turns":[132],"over\"":[133],"very":[134],"slowly":[135],"in":[136,147,159],"vivo.":[137],"The":[138],"not":[142,172],"detectably":[143],"32P-phosphate":[146],"vivo,":[148],"nor":[149],"are":[150],"immune":[151],"complexes":[152],"containing":[153],"associated":[155],"phosphokinase":[157],"activity":[158],"vitro.":[160],"Sequential":[161],"immunoprecipitation":[162],"studies":[166],"member":[174],"leukosialin/sialophorin":[177],"family":[178],"despite":[179],"fact":[181],"these":[183],"molecules":[184],"share":[185],"structural":[187],"similarities.":[188],"Partial":[189],"amino":[190],"acid":[191],"purified":[195],"revealed":[198],"no":[199],"significant":[200],"sequence":[201],"similarity":[202],"any":[204],"previously":[205],"described":[206],"structures.":[207]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}