{"id":"https://openalex.org/W1628293877","doi":"https://doi.org/10.1128/jvi.66.7.4407-4412.1992","title":"Expression of the OSU rotavirus outer capsid protein VP4 by an adenovirus recombinant","display_name":"Expression of the OSU rotavirus outer capsid protein VP4 by an adenovirus recombinant","publication_year":1992,"publication_date":"1992-07-01","ids":{"openalex":"https://openalex.org/W1628293877","doi":"https://doi.org/10.1128/jvi.66.7.4407-4412.1992","mag":"1628293877","pmid":"https://pubmed.ncbi.nlm.nih.gov/1318411","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/241248"},"language":"en","primary_location":{"id":"doi:10.1128/jvi.66.7.4407-4412.1992","doi":"https://doi.org/10.1128/jvi.66.7.4407-4412.1992","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.66.7.4407-4412.1992","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC241248","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044934436","display_name":"Mario I. Gorziglia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M Gorziglia","raw_affiliation_strings":["Epidemiology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Epidemiology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892","institution_ids":["https://openalex.org/I4210134534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111531281","display_name":"Albert Z. Kapikian","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A Z Kapikian","raw_affiliation_strings":["Epidemiology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Epidemiology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892","institution_ids":["https://openalex.org/I4210134534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210134534"],"apc_list":null,"apc_paid":null,"fwci":1.8149,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.84709322,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"66","issue":"7","first_page":"4407","last_page":"4412"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10976","display_name":"Viral gastroenteritis research and epidemiology","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10976","display_name":"Viral gastroenteritis research and epidemiology","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.019600000232458115,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/porcine-rotavirus","display_name":"porcine rotavirus","score":0.9539999961853027},{"id":"https://openalex.org/keywords/recombinant-adenovirus-type-5","display_name":"recombinant adenovirus type 5","score":0.9279999732971191},{"id":"https://openalex.org/keywords/rotavirus-vaccine","display_name":"rotavirus vaccine","score":0.6430000066757202},{"id":"https://openalex.org/keywords/neutralizing-antibodies","display_name":"neutralizing antibodies","score":0.9419999718666077},{"id":"https://openalex.org/keywords/booster-vaccination","display_name":"booster vaccination","score":0.8619999885559082},{"id":"https://openalex.org/keywords/intranasal-immunization","display_name":"intranasal immunization","score":0.6949999928474426},{"id":"https://openalex.org/keywords/sigmodon-hispidus","display_name":"Sigmodon hispidus","score":0.41200000047683716}],"concepts":[{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.8259615302085876},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7679765224456787},{"id":"https://openalex.org/C202878990","wikidata":"https://www.wikidata.org/wiki/Q830465","display_name":"Capsid","level":3,"score":0.7578694820404053},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7466143369674683},{"id":"https://openalex.org/C2781367151","wikidata":"https://www.wikidata.org/wiki/Q164778","display_name":"Rotavirus","level":3,"score":0.6535506844520569},{"id":"https://openalex.org/C2779261636","wikidata":"https://www.wikidata.org/wiki/Q7003054","display_name":"Neutralizing antibody","level":3,"score":0.6101164221763611},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.5257292985916138},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.5155884623527527},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5057082176208496},{"id":"https://openalex.org/C2779416561","wikidata":"https://www.wikidata.org/wiki/Q910576","display_name":"Reoviridae","level":4,"score":0.493163526058197},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.48879334330558777},{"id":"https://openalex.org/C2776954459","wikidata":"https://www.wikidata.org/wiki/Q193447","display_name":"Adenoviridae","level":4,"score":0.4293139576911926},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.3953252136707306},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14682337641716003},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07646486163139343},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000260","descriptor_name":"Adenoviruses, Human","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000914","descriptor_name":"Antibodies, Viral","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D002213","descriptor_name":"Capsid","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002213","descriptor_name":"Capsid","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002213","descriptor_name":"Capsid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012401","descriptor_name":"Rotavirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012401","descriptor_name":"Rotavirus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016205","descriptor_name":"Sigmodontinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016679","descriptor_name":"Genome, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036022","descriptor_name":"Capsid Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/jvi.66.7.4407-4412.1992","doi":"https://doi.org/10.1128/jvi.66.7.4407-4412.1992","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.66.7.4407-4412.1992","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:28804","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC241248","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:241248","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/241248","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},{"id":"pmid:1318411","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1318411","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:28804","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC241248","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W1514404799","https://openalex.org/W1516325674","https://openalex.org/W1524951584","https://openalex.org/W1577721257","https://openalex.org/W1595280335","https://openalex.org/W1815394689","https://openalex.org/W1824800369","https://openalex.org/W1956054686","https://openalex.org/W1965372133","https://openalex.org/W1989110977","https://openalex.org/W1992045035","https://openalex.org/W2011871276","https://openalex.org/W2018289835","https://openalex.org/W2018927042","https://openalex.org/W2036697340","https://openalex.org/W2039956487","https://openalex.org/W2044296969","https://openalex.org/W2051223657","https://openalex.org/W2059944938","https://openalex.org/W2077862111","https://openalex.org/W2093835870","https://openalex.org/W2100837269","https://openalex.org/W2102191410","https://openalex.org/W2104472592","https://openalex.org/W2114635621","https://openalex.org/W2134940049","https://openalex.org/W2145342678","https://openalex.org/W2161344127","https://openalex.org/W2180846871","https://openalex.org/W2371285051","https://openalex.org/W2410735561"],"related_works":["https://openalex.org/W2011871276","https://openalex.org/W2349669598","https://openalex.org/W2366719792","https://openalex.org/W2791264347","https://openalex.org/W2409742951","https://openalex.org/W2374305002","https://openalex.org/W3029715615","https://openalex.org/W2992286467","https://openalex.org/W2416425399","https://openalex.org/W902864168"],"abstract_inverted_index":{"Full-length":[0],"cDNA":[1],"of":[2,6,19,90,116,124,132,146],"the":[3,20,39,84,91,125,133,141],"VP4":[4,31,52,94,109,135,142],"gene":[5],"porcine":[7],"rotavirus":[8,32,69,148,166],"strain":[9,86,149],"OSU":[10,51,62,85,108],"was":[11,54,99,119],"cloned":[12],"into":[13],"adenovirus":[14,156],"type":[15],"5":[16],"(Ad5)":[17],"downstream":[18],"E3":[21],"promoter.":[22],"The":[23,36,96],"plaque-purified":[24],"recombinant":[25,155],"(Ad5-OSU":[26],"VP4)":[27],"expressed":[28,152],"apparently":[29],"authentic":[30],"outer":[33,143],"capsid":[34,144],"protein.":[35],"protein":[37,145],"had":[38],"same":[40],"molecular":[41],"size":[42],"(85":[43],"kDa)":[44],"and":[45,53,128],"electrophoretic":[46],"mobility":[47],"as":[48],"did":[49],"native":[50],"immunoprecipitated":[55],"by":[56,101,121,153],"a":[57,102,113,147,154],"polyclonal":[58],"antiserum":[59],"raised":[60],"to":[61],"VP4.":[63],"Cotton":[64],"rats":[65],"that":[66,71,140],"possessed":[67],"prechallenge":[68],"antibodies":[70,82,118],"may":[72],"have":[73],"been":[74],"acquired":[75],"either":[76],"passively":[77],"or":[78],"actively":[79],"developed":[80],"neutralizing":[81,97,117],"against":[83,165],"following":[87],"intranasal":[88,130],"administration":[89,123,131],"live":[92],"Ad5-OSU":[93,134],"recombinant.":[95,136],"activity":[98],"enhanced":[100],"parenteral":[103,122],"booster":[104],"injection":[105],"with":[106],"baculovirus-expressed":[107],"antigen.":[110],"In":[111],"addition,":[112],"high":[114],"titer":[115],"induced":[120],"latter":[126],"antigen":[127],"subsequent":[129],"These":[137],"observations":[138],"indicate":[139],"can":[150],"be":[151],"vector.":[157],"This":[158],"approach":[159],"warrants":[160],"further":[161],"exploration":[162],"for":[163],"immunization":[164],"disease.":[167]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}