{"id":"https://openalex.org/W162153387","doi":"https://doi.org/10.1182/blood.v96.1.100","title":"Adenoviral vector\u2013mediated gene transfer to primitive human hematopoietic progenitor cells: assessment of transduction and toxicity in long-term culture","display_name":"Adenoviral vector\u2013mediated gene transfer to primitive human hematopoietic progenitor cells: assessment of transduction and toxicity in long-term culture","publication_year":2000,"publication_date":"2000-07-01","ids":{"openalex":"https://openalex.org/W162153387","doi":"https://doi.org/10.1182/blood.v96.1.100","mag":"162153387","pmid":"https://pubmed.ncbi.nlm.nih.gov/10891437"},"language":"en","primary_location":{"id":"doi:10.1182/blood.v96.1.100","doi":"https://doi.org/10.1182/blood.v96.1.100","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v96.1.100","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026465234","display_name":"Karen L. MacKenzie","orcid":"https://orcid.org/0000-0003-2996-1617","observed_orcids":["https://orcid.org/0000-0003-2996-1617"]},"institutions":[{"id":"https://openalex.org/I1334819555","display_name":"Memorial Sloan Kettering Cancer Center","ror":"https://ror.org/02yrq0923","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1334819555"]},{"id":"https://openalex.org/I1340179700","display_name":"NewYork\u2013Presbyterian Hospital","ror":"https://ror.org/03gzbrs57","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1340179700"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karen L. MacKenzie","raw_affiliation_strings":["From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY","institution_ids":["https://openalex.org/I1334819555","https://openalex.org/I1340179700","https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001983513","display_name":"Neil R. Hackett","orcid":"https://orcid.org/0009-0006-9184-4437","observed_orcids":["https://orcid.org/0009-0006-9184-4437"]},"institutions":[{"id":"https://openalex.org/I1334819555","display_name":"Memorial Sloan Kettering Cancer Center","ror":"https://ror.org/02yrq0923","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1334819555"]},{"id":"https://openalex.org/I1340179700","display_name":"NewYork\u2013Presbyterian Hospital","ror":"https://ror.org/03gzbrs57","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1340179700"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Neil R. Hackett","raw_affiliation_strings":["From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY","institution_ids":["https://openalex.org/I1334819555","https://openalex.org/I1340179700","https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064433832","display_name":"Ronald G. Crystal","orcid":"https://orcid.org/0000-0001-6177-6839","observed_orcids":["https://orcid.org/0000-0001-6177-6839"]},"institutions":[{"id":"https://openalex.org/I1334819555","display_name":"Memorial Sloan Kettering Cancer Center","ror":"https://ror.org/02yrq0923","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1334819555"]},{"id":"https://openalex.org/I1340179700","display_name":"NewYork\u2013Presbyterian Hospital","ror":"https://ror.org/03gzbrs57","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1340179700"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ronald G. Crystal","raw_affiliation_strings":["From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY","institution_ids":["https://openalex.org/I1334819555","https://openalex.org/I1340179700","https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110863459","display_name":"Malcolm A.S. Moore","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1334819555","display_name":"Memorial Sloan Kettering Cancer Center","ror":"https://ror.org/02yrq0923","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1334819555"]},{"id":"https://openalex.org/I1340179700","display_name":"NewYork\u2013Presbyterian Hospital","ror":"https://ror.org/03gzbrs57","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1340179700"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Malcolm A. S. Moore","raw_affiliation_strings":["From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, NY, and the Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University\u2013New York Presbyterian Hospital, New York, NY","institution_ids":["https://openalex.org/I1334819555","https://openalex.org/I1340179700","https://openalex.org/I205783295"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":3.2929,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.9161519,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"96","issue":"1","first_page":"100","last_page":"108"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adenovirus-vectors","display_name":"adenoviral vector","score":0.9599999785423279},{"id":"https://openalex.org/keywords/hematopoietic-progenitor-cells","display_name":"hematopoietic progenitor cells","score":0.9679999947547913},{"id":"https://openalex.org/keywords/gene-therapy","display_name":"gene therapy","score":0.6940000057220459},{"id":"https://openalex.org/keywords/transduction-efficiency","display_name":"transduction efficiency","score":0.8820000290870667},{"id":"https://openalex.org/keywords/cd34-cells","display_name":"CD34+ cells","score":0.8569999933242798},{"id":"https://openalex.org/keywords/cobblestone-area-forming-cells","display_name":"cobblestone area-forming cells","score":0.8899999856948853},{"id":"https://openalex.org/keywords/colony-forming-units","display_name":"colony-forming units","score":0.8040000200271606},{"id":"https://openalex.org/keywords/clonogenicity","display_name":"clonogenicity","score":0.9359999895095825},{"id":"https://openalex.org/keywords/gfp-reporter","display_name":"GFP reporter","score":0.6620000004768372},{"id":"https://openalex.org/keywords/long-term-culture","display_name":"long-term culture","score":0.7080000042915344}],"concepts":[{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.8283912539482117},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7148825526237488},{"id":"https://openalex.org/C117262875","wikidata":"https://www.wikidata.org/wiki/Q5135057","display_name":"Clonogenic assay","level":3,"score":0.6140403747558594},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.5991924405097961},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.5872204303741455},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.526885986328125},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.526694655418396},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.5239762663841248},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4926448464393616},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4824562668800354},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.45276087522506714},{"id":"https://openalex.org/C10205521","wikidata":"https://www.wikidata.org/wiki/Q4035578","display_name":"CD34","level":3,"score":0.42209795117378235},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.4149115979671478},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.41355279088020325},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2074601948261261},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.20011112093925476},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1265556812286377},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12349343299865723},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.0790230929851532}],"mesh":[{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000943","descriptor_name":"Antigens, Differentiation","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003114","descriptor_name":"Colony-Forming Units Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009244","descriptor_name":"NAD+ Nucleosidase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015703","descriptor_name":"Antigens, CD","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018952","descriptor_name":"Antigens, CD34","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D019650","descriptor_name":"Hematopoietic Stem Cell Mobilization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036541","descriptor_name":"ADP-ribosyl Cyclase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051997","descriptor_name":"ADP-ribosyl Cyclase 1","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1182/blood.v96.1.100","doi":"https://doi.org/10.1182/blood.v96.1.100","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v96.1.100","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},{"id":"doi:10.1182/blood.v96.1.100.013k41_100_108","doi":"https://doi.org/10.1182/blood.v96.1.100.013k41_100_108","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v96.1.100.013k41_100_108","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},{"id":"pmid:10891437","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10891437","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9763000011444092,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G546047228","display_name":null,"funder_award_id":"CA08748","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000856728","display_name":"Cancer Center Support Grant","funder_award_id":"P30CA008748","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W41807445","https://openalex.org/W159317543","https://openalex.org/W203852859","https://openalex.org/W1481112340","https://openalex.org/W1512478116","https://openalex.org/W1528496162","https://openalex.org/W1545476994","https://openalex.org/W1566385126","https://openalex.org/W1566567127","https://openalex.org/W1587940136","https://openalex.org/W1599323293","https://openalex.org/W1840943005","https://openalex.org/W1965200427","https://openalex.org/W1998397323","https://openalex.org/W2019890805","https://openalex.org/W2020515612","https://openalex.org/W2039843910","https://openalex.org/W2040827928","https://openalex.org/W2051758487","https://openalex.org/W2065392104","https://openalex.org/W2067908161","https://openalex.org/W2082795668","https://openalex.org/W2105007068","https://openalex.org/W2107199882","https://openalex.org/W2114458218","https://openalex.org/W2116071714","https://openalex.org/W2116814822","https://openalex.org/W2122062489","https://openalex.org/W2130137650","https://openalex.org/W2133388585","https://openalex.org/W2134045449","https://openalex.org/W2160509246","https://openalex.org/W2197012898","https://openalex.org/W2257934670","https://openalex.org/W2259098203","https://openalex.org/W2286095548","https://openalex.org/W2305289606","https://openalex.org/W2311232153","https://openalex.org/W2339685543","https://openalex.org/W2340284708","https://openalex.org/W2415445296","https://openalex.org/W2417385986","https://openalex.org/W2605108092"],"related_works":["https://openalex.org/W1967010387","https://openalex.org/W1971736076","https://openalex.org/W2417385986","https://openalex.org/W203852859","https://openalex.org/W2016894365","https://openalex.org/W1999472959","https://openalex.org/W2171052557","https://openalex.org/W2081880097","https://openalex.org/W1984919877","https://openalex.org/W2329863069"],"abstract_inverted_index":{"Adenoviral":[0,160],"gene":[1,13,226],"transfer":[2],"to":[3,39,77,209],"primitive":[4,43,87,130,168,172,201,214],"hematopoietic":[5],"progenitor":[6],"cells":[7,64,116,148,173],"(HPCs)":[8],"would":[9],"be":[10,207,220],"useful":[11],"in":[12,49,139],"therapy":[14],"applications":[15],"where":[16],"transient,":[17],"high-level":[18],"transgene":[19],"expression":[20],"is":[21,174],"required.":[22],"In":[23],"the":[24,34,142,164,191],"present":[25],"investigations,":[26],"we":[27,53],"have":[28],"used":[29],"an":[30],"adenoviral":[31,198,211,217],"vector":[32],"expressing":[33],"green":[35,97],"fluorescent":[36],"protein":[37,99],"(AdGFP)":[38],"quantify":[40],"transduction":[41,74,104,199],"of":[42,62,75,79,83,105,107,111,120,128,145,167,171,197,200],"HPCs":[44,88,215],"and":[45,65,85,118,181,194],"assess":[46],"adenoviral-associated":[47],"toxicity":[48,161,212],"long-term":[50],"culture.":[51],"Here":[52],"show":[54],"that":[55],"a":[56,222],"cytokine":[57],"cocktail":[58],"protects":[59],"mass":[60],"populations":[61],"CD34(+)":[63,80],"primary":[66,108],"colony":[67],"forming":[68,115],"unit-cultures":[69],"(CFU-Cs)":[70],"from":[71],"toxicity,":[72],"enabling":[73],"up":[76],"79%":[78],"cells.":[81,131,159],"Transduction":[82,170],"CFU-Cs":[84,180],"more":[86,129,182],"was":[89,134,149],"quantified":[90],"following":[91],"fluorescence":[92],"activated":[93],"cell":[94],"sorting":[95],"for":[96,225],"flourescence":[98],"expression.":[100],"Our":[101],"results":[102],"demonstrate":[103],"45%":[106],"CFU-Cs,":[109],"33%":[110],"week-5":[112,121,140,146,179,186],"cobblestone":[113],"area":[114],"(CAFCs),":[117],"18%":[119],"CFU-Cs.":[122],"However,":[123,203],"AdGFP":[124],"infection":[125],"inhibited":[126],"proliferation":[127],"Although":[132],"there":[133],"no":[135],"apparent":[136],"quantitative":[137,195],"change":[138],"CAFCs,":[141],"clonogenic":[143],"capacity":[144],"AdGFP-infected":[147],"reduced":[150],"by":[151,178,185],"40%":[152],"(P":[153],"<.01)":[154],"when":[155],"compared":[156],"with":[157],"mock-infected":[158],"specifically":[162],"affected":[163],"transduced":[165],"subset":[166],"HPCs.":[169,202],"therefore":[175],"probably":[176],"underestimated":[177],"accurately":[183],"indicated":[184],"CAFCs.":[187],"These":[188],"studies":[189],"provide":[190],"first":[192],"functional":[193],"evidence":[196],"further":[204],"investigations":[205],"will":[206],"necessary":[208],"overcome":[210],"toward":[213],"before":[216],"vectors":[218],"can":[219],"considered":[221],"safe":[223],"option":[224],"therapy.":[227]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
