{"id":"https://openalex.org/W1607120100","doi":"https://doi.org/10.1016/s0021-9258(19)39706-6","title":"Site-directed mutagenesis of the -10 region of the lacUV5 promoter. Introduction of dA4.dT4 tract suppresses open complex formation.","display_name":"Site-directed mutagenesis of the -10 region of the lacUV5 promoter. Introduction of dA4.dT4 tract suppresses open complex formation.","publication_year":1990,"publication_date":"1990-03-01","ids":{"openalex":"https://openalex.org/W1607120100","doi":"https://doi.org/10.1016/s0021-9258(19)39706-6","mag":"1607120100","pmid":"https://pubmed.ncbi.nlm.nih.gov/2406269"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)39706-6","doi":"https://doi.org/10.1016/s0021-9258(19)39706-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)39706-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)39706-6","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"P T Chan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P T Chan","raw_affiliation_strings":["Department of Microbiology, University of Alabama, Birmingham, 35294"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Alabama, Birmingham, 35294","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025309149","display_name":"Jacob Lebowitz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Lebowitz","raw_affiliation_strings":["Department of Microbiology, University of Alabama, Birmingham, 35294"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Alabama, Birmingham, 35294","institution_ids":["https://openalex.org/I32389192"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I32389192"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.1425,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.40511132,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"265","issue":"7","first_page":"4091","last_page":"4097"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.5189999938011169,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.5189999938011169,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.31690001487731934,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.13940000534057617,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lacuv5-promoter","display_name":"lacUV5 promoter","score":0.9950000047683716},{"id":"https://openalex.org/keywords/tata-box","display_name":"TATA box","score":0.7239999771118164},{"id":"https://openalex.org/keywords/open-complex-formation","display_name":"open complex formation","score":0.7910000085830688},{"id":"https://openalex.org/keywords/rna-polymerase-binding","display_name":"RNA polymerase binding","score":0.8970000147819519},{"id":"https://openalex.org/keywords/dna-bending","display_name":"DNA bending","score":0.5450000166893005},{"id":"https://openalex.org/keywords/dnase-i-footprinting","display_name":"DNase I footprinting","score":0.8429999947547913},{"id":"https://openalex.org/keywords/heparin-binding","display_name":"heparin binding","score":0.7319999933242798},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7549999952316284}],"concepts":[{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.7014355659484863},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.6167491674423218},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.43781036138534546},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41356828808784485},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40748950839042664},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.37871286273002625},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34141480922698975},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.33328956365585327},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.23913049697875977},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2071458101272583}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003850","descriptor_name":"Deoxyribonuclease I","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009710","descriptor_name":"Nucleotide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011061","descriptor_name":"Poly A","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011071","descriptor_name":"Poly T","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)39706-6","doi":"https://doi.org/10.1016/s0021-9258(19)39706-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)39706-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2406269","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2406269","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)39706-6","doi":"https://doi.org/10.1016/s0021-9258(19)39706-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)39706-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5123181962","display_name":null,"funder_award_id":"CA17077","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6628610537","display_name":"TRAINING PROGRAM IN MOLECULAR AND VIRAL ONCOLOGY","funder_award_id":"T32CA009467","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G737609964","display_name":null,"funder_award_id":"CA 09467","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G8115736295","display_name":"CHEMICAL INHIBITION OF TRANSCRIPTION &DNA SUPERCOILING","funder_award_id":"5r01ca017077-12","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W135355514","https://openalex.org/W597006680","https://openalex.org/W1558516755","https://openalex.org/W1604842333","https://openalex.org/W1690413094","https://openalex.org/W1963658914","https://openalex.org/W1982375617","https://openalex.org/W1992595091","https://openalex.org/W1995065291","https://openalex.org/W1996261926","https://openalex.org/W1996642589","https://openalex.org/W2009605380","https://openalex.org/W2010777538","https://openalex.org/W2016607295","https://openalex.org/W2019410656","https://openalex.org/W2019895587","https://openalex.org/W2028622989","https://openalex.org/W2030551247","https://openalex.org/W2036272159","https://openalex.org/W2037537999","https://openalex.org/W2037898928","https://openalex.org/W2038874225","https://openalex.org/W2041975656","https://openalex.org/W2042307633","https://openalex.org/W2044952215","https://openalex.org/W2050042672","https://openalex.org/W2054468433","https://openalex.org/W2068825915","https://openalex.org/W2071424950","https://openalex.org/W2071765639","https://openalex.org/W2071872098","https://openalex.org/W2080616338","https://openalex.org/W2084565432","https://openalex.org/W2088398722","https://openalex.org/W2129228470","https://openalex.org/W2138270253","https://openalex.org/W2140158396","https://openalex.org/W2146784596","https://openalex.org/W2155737027"],"related_works":["https://openalex.org/W1604842333","https://openalex.org/W1565453664","https://openalex.org/W1873417362","https://openalex.org/W2059168674","https://openalex.org/W2111662779","https://openalex.org/W2015007275","https://openalex.org/W2036328069","https://openalex.org/W2013524765","https://openalex.org/W2007452681","https://openalex.org/W3173112085"],"abstract_inverted_index":{"Homopolymeric":[0],"dAn.dTn":[1],"sequences,":[2],"where":[3],"n":[4],"is":[5],"4":[6],"or":[7,156],"greater,":[8],"have":[9],"special":[10],"properties":[11],"leading":[12],"to":[13,25,37,129,141],"increased":[14,162],"duplex":[15],"stability":[16],"and":[17,68,108,140,161],"DNA":[18],"bending.":[19],"The":[20,41],"lacUV5":[21,107,131,148,174],"promoter":[22,124,134,149,167,175],"was":[23,48],"used":[24],"examine":[26],"the":[27,32,38,45,57,66,73,95,106,130,146],"functional":[28],"consequences":[29],"of":[30,72,159],"changing":[31],"-10":[33],"TATAAT":[34],"consensus":[35],"sequence":[36,39,97],"TAAAAT.":[40],"transversion":[42],"mutation":[43],"at":[44,65],"underlined":[46],"site":[47,85],"accomplished":[49],"with":[50,154],"site-directed":[51],"mutagenesis":[52],"using":[53],"translation":[54],"termination":[55],"as":[56],"selection":[58],"procedure.":[59],"For":[60],"free":[61],"DNA,":[62],"structural":[63],"differences":[64],"5'":[67],"3'":[69],"junction":[70],"regions":[71],"dA4.dT4":[74],"tract":[75],"can":[76],"be":[77],"readily":[78],"detected":[79],"by":[80,87,94],"DNase":[81,100],"I":[82,101],"digestion.":[83],"However,":[84],"binding":[86],"Escherichia":[88],"coli":[89],"RNA":[90],"polymerase":[91],"appeared":[92],"unaltered":[93],"TAAAAT":[96],"since":[98],"identical":[99],"footprints":[102],"were":[103],"obtained":[104],"for":[105],"mutant":[109,123,166],"promoters.":[110],"Binding":[111],"competition":[112],"studies":[113],"under":[114],"different":[115],"ionic":[116],"strengths":[117],"revealed":[118],"a":[119,142,157],"significant":[120],"reduction":[121],"in":[122],"open":[125,135,150,168,176],"complex":[126],"formation":[127],"relative":[128],"promoter.":[132],"Mutant":[133],"complexes":[136,151,169],"also":[137],"dissociated":[138],"faster":[139],"greater":[143],"extent":[144],"than":[145,173],"corresponding":[147],"when":[152],"challenged":[153],"heparin":[155,160],"combination":[158],"KCl":[163],"concentration.":[164],"Consequently,":[165],"appear":[170],"less":[171],"stable":[172],"complexes.":[177]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}