{"id":"https://openalex.org/W1604447247","doi":"https://doi.org/10.1016/s0021-9258(18)48078-7","title":"Visualization of the homologous pairing of DNA catalyzed by the bacteriophage T4 UvsX protein.","display_name":"Visualization of the homologous pairing of DNA catalyzed by the bacteriophage T4 UvsX protein.","publication_year":1987,"publication_date":"1987-07-01","ids":{"openalex":"https://openalex.org/W1604447247","doi":"https://doi.org/10.1016/s0021-9258(18)48078-7","mag":"1604447247","pmid":"https://pubmed.ncbi.nlm.nih.gov/3496334"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)48078-7","doi":"https://doi.org/10.1016/s0021-9258(18)48078-7","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)48078-7","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)48078-7","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5069751226","display_name":"Lorelei D. Harris","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"L D Harris","raw_affiliation_strings":["From the Lineberger Cancer Research Center and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Lineberger Cancer Research Center and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5061660001","display_name":"Jack D. Griffith","orcid":"https://orcid.org/0000-0002-8357-8689","observed_orcids":["https://orcid.org/0000-0002-8357-8689"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Griffith","raw_affiliation_strings":["From the Lineberger Cancer Research Center and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Lineberger Cancer Research Center and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.3763,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.80526206,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"262","issue":"19","first_page":"9285","last_page":"9292"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.00419999985024333,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacteriophage-t4","display_name":"bacteriophage T4","score":0.9789999723434448},{"id":"https://openalex.org/keywords/homologous-pairing","display_name":"homologous pairing","score":0.8830000162124634},{"id":"https://openalex.org/keywords/single-stranded-dna","display_name":"single-stranded DNA","score":0.8429999947547913},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.5569999814033508},{"id":"https://openalex.org/keywords/d-loop","display_name":"D-loop","score":0.5099999904632568},{"id":"https://openalex.org/keywords/reca","display_name":"RecA","score":0.5839999914169312},{"id":"https://openalex.org/keywords/dna-topology","display_name":"DNA topology","score":0.45399999618530273}],"concepts":[{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.7743213176727295},{"id":"https://openalex.org/C14228908","wikidata":"https://www.wikidata.org/wiki/Q2920483","display_name":"Protein filament","level":2,"score":0.7575877904891968},{"id":"https://openalex.org/C14103023","wikidata":"https://www.wikidata.org/wiki/Q11681459","display_name":"Pairing","level":3,"score":0.6218389868736267},{"id":"https://openalex.org/C165525559","wikidata":"https://www.wikidata.org/wiki/Q224180","display_name":"Homology (biology)","level":3,"score":0.5929923057556152},{"id":"https://openalex.org/C64894306","wikidata":"https://www.wikidata.org/wiki/Q849622","display_name":"Homologous chromosome","level":3,"score":0.5824808478355408},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.5333585143089294},{"id":"https://openalex.org/C185581394","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Base pair","level":3,"score":0.5234431624412537},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.514712393283844},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.479289710521698},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.44430550932884216},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.44375577569007874},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.43834373354911804},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.37573084235191345},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3363657593727112},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3357497453689575},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.30609747767448425},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2295096218585968},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.13673335313796997},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C54101563","wikidata":"https://www.wikidata.org/wiki/Q124131","display_name":"Superconductivity","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004277","descriptor_name":"DNA, Single-Stranded","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004587","descriptor_name":"Electrophoresis, Agar Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008855","descriptor_name":"Microscopy, Electron, Scanning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D013604","descriptor_name":"T-Phages","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)48078-7","doi":"https://doi.org/10.1016/s0021-9258(18)48078-7","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)48078-7","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:3496334","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3496334","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)48078-7","doi":"https://doi.org/10.1016/s0021-9258(18)48078-7","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)48078-7","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1682855642","display_name":null,"funder_award_id":"CA-160-86","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G2774293502","display_name":null,"funder_award_id":"GM-31819","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G3757727673","display_name":"NRSA in Genetics","funder_award_id":"T32GM007092","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W1482095729","https://openalex.org/W1491721292","https://openalex.org/W1494772491","https://openalex.org/W1512455821","https://openalex.org/W1544096350","https://openalex.org/W1551887231","https://openalex.org/W1556041268","https://openalex.org/W1577415753","https://openalex.org/W1586856143","https://openalex.org/W1600553076","https://openalex.org/W1600572106","https://openalex.org/W1605643455","https://openalex.org/W1607722677","https://openalex.org/W1646166592","https://openalex.org/W1920374602","https://openalex.org/W1963653634","https://openalex.org/W1966004046","https://openalex.org/W1971198145","https://openalex.org/W2007114583","https://openalex.org/W2007977422","https://openalex.org/W2020464460","https://openalex.org/W2025509435","https://openalex.org/W2037320704","https://openalex.org/W2040597566","https://openalex.org/W2049355261","https://openalex.org/W2078354583","https://openalex.org/W2079966173","https://openalex.org/W2083310350","https://openalex.org/W2095055244","https://openalex.org/W2121813899","https://openalex.org/W2887458816"],"related_works":["https://openalex.org/W2007208908","https://openalex.org/W1920374602","https://openalex.org/W1577415753","https://openalex.org/W1544096350","https://openalex.org/W1636197238","https://openalex.org/W2062061915","https://openalex.org/W1564751166","https://openalex.org/W1607722677","https://openalex.org/W1966341579","https://openalex.org/W1969733152"],"abstract_inverted_index":{"The":[0,15,230],"uvsX":[1],"gene":[2],"product":[3],"is":[4],"essential":[5],"for":[6,223],"DNA":[7,27,31],"repair":[8],"and":[9,44,130,154,170],"general":[10],"recombination":[11],"in":[12,33,102,209,239,251],"T4":[13],"bacteriophage.":[14],"ability":[16],"of":[17,25,54,57,86,100,118,136,149,177,184,188,198,204,226,232,258],"UvsX":[18,55,70,112,220],"protein":[19,71,257],"to":[20,95,110,128,142,193,249],"catalyze":[21],"the":[22,64,77,84,143,168,171,185,189,199,202,205,210,219,233,255],"homologous":[23,90,151],"pairing":[24,85],"single-stranded":[26],"(ssDNA)":[28],"with":[29,89,218],"double-stranded":[30],"(dsDNA)":[32],"vitro":[34],"was":[35,50,66,93,108,235],"examined":[36],"by":[37,69,74,163,181],"electron":[38],"microscopic":[39],"(EM),":[40],"nitrocellulose":[41],"filter":[42],"binding,":[43],"gel":[45],"electrophoretic":[46],"methods.":[47],"Optimal":[48],"joining":[49],"observed":[51],"at":[52,175],"ratios":[53],"protein:ssDNA":[56],"2":[58],"nucleotides/protein":[59],"monomer.":[60],"At":[61],"this":[62,82,240],"level,":[63],"ssDNA":[65,88,138,153],"fully":[67,150],"covered":[68],"as":[72,161,179],"seen":[73,162],"EM,":[75],"while":[76],"dsDNA":[78,92,106,144,156,215],"appeared":[79],"protein-free.":[80],"Using":[81],"stoichiometry,":[83],"circular":[87],"supertwisted":[91,105,155],"found":[94],"produce":[96],"a":[97,104,111,116,212,224],"high":[98],"frequency":[99],"complexes":[101,191],"which":[103],"molecule":[107],"joined":[109,190],"protein-ssDNA":[113,221],"filament":[114,222,234],"over":[115],"distance":[117,225],"less":[119],"than":[120],"100":[121],"base":[122,228],"pairs.":[123,229],"These":[124,242],"joints":[125,159,173,200],"were":[126,174],"labile":[127],"deproteinization":[129,194],"must":[131],"have":[132],"been":[133],"paranemic.":[134],"Pairing":[135,148],"linear":[137,152],"containing":[139],"buried":[140],"homology":[141],"produced":[145],"identical":[146],"structures.":[147],"yielded":[157],"D-loop":[158],"(plectonemic)":[160],"EM":[164],"following":[165],"deproteinization.":[166],"Both":[167],"paranemic":[169,206],"plectonemic":[172],"sites":[176],"homology,":[178],"demonstrated":[180],"restriction":[182],"cleavage":[183],"complexes.":[186],"Visualization":[187],"prior":[192],"showed":[195],"that":[196],"50%":[197],"had":[201],"architecture":[203],"joints,":[207],"whereas":[208],"remainder,":[211],"topologically":[213],"relaxed":[214],"circle":[216],"merged":[217],"450":[227],"structure":[231],"not":[236,247],"visibly":[237],"altered":[238],"region.":[241],"observations":[243],"are":[244],"similar,":[245],"but":[246],"identical,":[248],"findings":[250],"parallel":[252],"studies":[253],"utilizing":[254],"RecA":[256],"Escherichia":[259],"coli.":[260]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}