{"id":"https://openalex.org/W160059692","doi":null,"title":"Newly developed encapsulation-dehydration protocol for plantcryopreservation.","display_name":"Newly developed encapsulation-dehydration protocol for plantcryopreservation.","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W160059692","mag":"160059692","pmid":"https://pubmed.ncbi.nlm.nih.gov/12148065"},"language":"en","primary_location":{"id":"pmid:12148065","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12148065","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cryo letters","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5052455821","display_name":"Akira Sakai","orcid":"https://orcid.org/0000-0003-0943-7842","observed_orcids":["https://orcid.org/0000-0003-0943-7842"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"A Sakai","raw_affiliation_strings":["Hokkaido University, Asabucho 1-5-23, Kitaku, Sapporo, 001-0045 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hokkaido University, Asabucho 1-5-23, Kitaku, Sapporo, 001-0045 Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066248010","display_name":"Toshikazu Matsumoto","orcid":"https://orcid.org/0000-0003-1043-7727","observed_orcids":["https://orcid.org/0000-0003-1043-7727"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Matsumoto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"D Hirai","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Hirai","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5112410546","display_name":"T. Niino","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Niino","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":5.9772,"has_fulltext":false,"cited_by_count":53,"citation_normalized_percentile":{"value":0.95432924,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"21","issue":"1","first_page":"53","last_page":"62"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12834","display_name":"Flowering Plant Growth and Cultivation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plant-cryopreservation","display_name":"plant cryopreservation","score":0.9909999966621399},{"id":"https://openalex.org/keywords/encapsulation-dehydration","display_name":"encapsulation-dehydration","score":0.9629999995231628},{"id":"https://openalex.org/keywords/vitrification","display_name":"vitrification","score":0.7630000114440918},{"id":"https://openalex.org/keywords/shoot-tips","display_name":"shoot tips","score":0.7480000257492065},{"id":"https://openalex.org/keywords/osmoprotection","display_name":"osmoprotection","score":0.9580000042915344},{"id":"https://openalex.org/keywords/glycerol","display_name":"glycerol","score":0.8690000176429749},{"id":"https://openalex.org/keywords/sucrose","display_name":"sucrose","score":0.9020000100135803},{"id":"https://openalex.org/keywords/silica-gel-dehydration","display_name":"silica gel dehydration","score":0.9380000233650208},{"id":"https://openalex.org/keywords/recovery-growth","display_name":"recovery growth","score":0.7710000276565552},{"id":"https://openalex.org/keywords/wasabi","display_name":"wasabi","score":0.9750000238418579},{"id":"https://openalex.org/keywords/chrysanthemum","display_name":"chrysanthemum","score":1.0},{"id":"https://openalex.org/keywords/mint","display_name":"Mentha","score":0.9990000128746033}],"concepts":[{"id":"https://openalex.org/C2778022349","wikidata":"https://www.wikidata.org/wiki/Q1072553","display_name":"Vitrification","level":2,"score":0.8762620091438293},{"id":"https://openalex.org/C2778769901","wikidata":"https://www.wikidata.org/wiki/Q194290","display_name":"Dehydration","level":2,"score":0.8352319598197937},{"id":"https://openalex.org/C179933525","wikidata":"https://www.wikidata.org/wiki/Q1144664","display_name":"Cryopreservation","level":3,"score":0.7849694490432739},{"id":"https://openalex.org/C2780881558","wikidata":"https://www.wikidata.org/wiki/Q132501","display_name":"Glycerol","level":2,"score":0.6120225787162781},{"id":"https://openalex.org/C2778597767","wikidata":"https://www.wikidata.org/wiki/Q4027534","display_name":"Sucrose","level":2,"score":0.6096117496490479},{"id":"https://openalex.org/C81147070","wikidata":"https://www.wikidata.org/wiki/Q1172449","display_name":"Encapsulation (networking)","level":2,"score":0.5514897704124451},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5052128434181213},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2435784935951233},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.16637107729911804},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15654712915420532},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.09477686882019043},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.07523837685585022},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.05790853500366211},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.0},{"id":"https://openalex.org/C31258907","wikidata":"https://www.wikidata.org/wiki/Q1301371","display_name":"Computer network","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmid:12148065","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12148065","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cryo letters","raw_type":"Journal Article"},{"id":"mag:160059692","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12148065","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.40450000762939453,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1519927339","https://openalex.org/W1119897934","https://openalex.org/W1995587775","https://openalex.org/W1973738685","https://openalex.org/W1157432255","https://openalex.org/W32497421","https://openalex.org/W2184306112","https://openalex.org/W1998700400","https://openalex.org/W156866293","https://openalex.org/W2058296896"],"abstract_inverted_index":{"A":[0],"simplified":[1],"and":[2,12,95,127],"efficient":[3],"encapsulation-dehydration":[4],"protocol,":[5,21],"which":[6],"is":[7],"a":[8,40,65],"compromise":[9],"between":[10],"vitrification":[11],"encapsulation-dehydration,":[13],"was":[14],"presented":[15],"for":[16,33,50,75,112,122],"plant":[17,90],"cryopreservation.":[18],"In":[19],"this":[20,117],"during":[22],"the":[23,26,70,88,101,109,113],"encapsulation":[24],"process,":[25],"apices":[27,55,126],"precultured":[28],"with":[29,39,59],"0.3":[30],"M":[31,44,48],"sucrose":[32,49,74],"16":[34,76],"h":[35],"were":[36,56],"simultaneously":[37],"osmoprotected":[38],"mixture":[41],"of":[42,72,84,124],"02":[43],"glycerol":[45],"plus":[46],"0.4":[47],"1":[51],"h.":[52,77],"These":[53],"encapsulated":[54],"directly":[57],"dehydrated":[58],"dry":[60],"silica":[61],"gel":[62],"prior":[63],"to":[64],"plunge":[66],"into":[67],"LN":[68],"without":[69],"pretreatment":[71],"0.8M":[73],"This":[78,104],"protocol":[79,105,119],"produced":[80],"much":[81],"higher":[82],"rates":[83],"recovery":[85],"growth":[86],"in":[87],"three":[89],"species":[91],"tested":[92],"(wasabi,":[93],"chrysanthemum,":[94],"mint)":[96],"than":[97],"those":[98],"cryopreserved":[99],"by":[100],"conventional":[102],"encapsulation-dehydration.":[103],"also":[106],"considerably":[107],"reduced":[108],"time":[110],"needed":[111],"cryogenic":[114],"procedure.":[115],"Thus,":[116],"new":[118],"appears":[120],"promising":[121],"cryopreservation":[123],"shoot":[125],"other":[128],"explants.":[129]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}