{"id":"https://openalex.org/W1597063734","doi":null,"title":"Characterization of resuscitation promoting factors in Mycobacterium smegmatis","display_name":"Characterization of resuscitation promoting factors in Mycobacterium smegmatis","publication_year":2012,"publication_date":"2012-09-12","ids":{"openalex":"https://openalex.org/W1597063734","mag":"1597063734"},"language":"en","primary_location":{"id":"pmh:oai:wiredspace.wits.ac.za:10539/11937","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10539/11937","pdf_url":"http://hdl.handle.net/10539/11937","source":{"id":"https://openalex.org/S4306400603","display_name":"University of the Witwatersrand, Johannesburg Institutional Repository on DSpace (University of the Witwatersrand, Johannesburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I192619145","host_organization_name":"University of the Witwatersrand","host_organization_lineage":["https://openalex.org/I192619145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10539/11937","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058012852","display_name":"Lusanda Mapela","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Lusanda Mapela","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5058012852"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11316","display_name":"Mycobacterium research and diagnosis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mycobacterium-smegmatis","display_name":"Mycobacterium smegmatis","score":0.9380000233650208},{"id":"https://openalex.org/keywords/resuscitation-promoting-factor","display_name":"resuscitation-promoting factor","score":0.9890000224113464},{"id":"https://openalex.org/keywords/rpfb","display_name":"RpfB","score":0.9950000047683716},{"id":"https://openalex.org/keywords/peptidoglycan-cleavage","display_name":"peptidoglycan cleavage","score":0.796999990940094},{"id":"https://openalex.org/keywords/bacterial-dormancy","display_name":"bacterial dormancy","score":0.8309999704360962},{"id":"https://openalex.org/keywords/latent-tuberculosis-infection","display_name":"latent tuberculosis infection","score":0.7829999923706055},{"id":"https://openalex.org/keywords/biofilm-formation","display_name":"biofilm formation","score":0.7760000228881836},{"id":"https://openalex.org/keywords/cell-morphology","display_name":"cell morphology","score":0.5960000157356262},{"id":"https://openalex.org/keywords/antibiotic-susceptibility","display_name":"antibiotic susceptibility","score":0.621999979019165},{"id":"https://openalex.org/keywords/cell-wall-hydrolases","display_name":"cell wall hydrolases","score":0.6309999823570251},{"id":"https://openalex.org/keywords/deletion-mutants","display_name":"deletion mutants","score":0.4869999885559082},{"id":"https://openalex.org/keywords/bacterial-aggregation","display_name":"bacterial aggregation","score":0.47099998593330383},{"id":"https://openalex.org/keywords/virulence-factors","display_name":"virulence factors","score":0.40799999237060547}],"concepts":[{"id":"https://openalex.org/C2777204207","wikidata":"https://www.wikidata.org/wiki/Q606230","display_name":"Mycobacterium smegmatis","level":4,"score":0.8951934576034546},{"id":"https://openalex.org/C2778165595","wikidata":"https://www.wikidata.org/wiki/Q5491454","display_name":"Resuscitation","level":2,"score":0.5185114145278931},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4654119312763214},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.4159850478172302},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34060919284820557},{"id":"https://openalex.org/C2777975735","wikidata":"https://www.wikidata.org/wiki/Q130971","display_name":"Mycobacterium tuberculosis","level":3,"score":0.2544006109237671},{"id":"https://openalex.org/C194828623","wikidata":"https://www.wikidata.org/wiki/Q2861470","display_name":"Emergency medicine","level":1,"score":0.10581988096237183},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.09023669362068176},{"id":"https://openalex.org/C2781069245","wikidata":"https://www.wikidata.org/wiki/Q12204","display_name":"Tuberculosis","level":2,"score":0.07157644629478455}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:wiredspace.wits.ac.za:10539/11937","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10539/11937","pdf_url":"http://hdl.handle.net/10539/11937","source":{"id":"https://openalex.org/S4306400603","display_name":"University of the Witwatersrand, Johannesburg Institutional Repository on DSpace (University of the Witwatersrand, Johannesburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I192619145","host_organization_name":"University of the Witwatersrand","host_organization_lineage":["https://openalex.org/I192619145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:1597063734","doi":null,"is_oa":false,"landing_page_url":"https://wiredspace.wits.ac.za/handle/10539/11937?show=full","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:wiredspace.wits.ac.za:10539/11937","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10539/11937","pdf_url":"http://hdl.handle.net/10539/11937","source":{"id":"https://openalex.org/S4306400603","display_name":"University of the Witwatersrand, Johannesburg Institutional Repository on DSpace (University of the Witwatersrand, Johannesburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I192619145","host_organization_name":"University of the Witwatersrand","host_organization_lineage":["https://openalex.org/I192619145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1597063734.pdf","grobid_xml":"https://content.openalex.org/works/W1597063734.grobid-xml"},"referenced_works_count":123,"referenced_works":["https://openalex.org/W66300265","https://openalex.org/W150539660","https://openalex.org/W1495604208","https://openalex.org/W1580808235","https://openalex.org/W1594511007","https://openalex.org/W1657643071","https://openalex.org/W1662338722","https://openalex.org/W1833541179","https://openalex.org/W1847996750","https://openalex.org/W1899765966","https://openalex.org/W1910583010","https://openalex.org/W1928980316","https://openalex.org/W1965726711","https://openalex.org/W1967457339","https://openalex.org/W1967908436","https://openalex.org/W1969633963","https://openalex.org/W1970853823","https://openalex.org/W1974359669","https://openalex.org/W1984107105","https://openalex.org/W1990137967","https://openalex.org/W1994259898","https://openalex.org/W1995786329","https://openalex.org/W1998445790","https://openalex.org/W2002991152","https://openalex.org/W2003114988","https://openalex.org/W2004224847","https://openalex.org/W2009032605","https://openalex.org/W2010184230","https://openalex.org/W2011107270","https://openalex.org/W2011971929","https://openalex.org/W2012579922","https://openalex.org/W2013268532","https://openalex.org/W2024708232","https://openalex.org/W2028224799","https://openalex.org/W2030837263","https://openalex.org/W2031292535","https://openalex.org/W2034194686","https://openalex.org/W2035295085","https://openalex.org/W2036332517","https://openalex.org/W2036448942","https://openalex.org/W2036795963","https://openalex.org/W2038101863","https://openalex.org/W2039820790","https://openalex.org/W2040316205","https://openalex.org/W2040817861","https://openalex.org/W2042130810","https://openalex.org/W2044879237","https://openalex.org/W2044996120","https://openalex.org/W2045825103","https://openalex.org/W2046559260","https://openalex.org/W2049526353","https://openalex.org/W2051114496","https://openalex.org/W2052779789","https://openalex.org/W2053048622","https://openalex.org/W2054270339","https://openalex.org/W2058208662","https://openalex.org/W2060966727","https://openalex.org/W2061953031","https://openalex.org/W2064521374","https://openalex.org/W2065769916","https://openalex.org/W2071330750","https://openalex.org/W2071478811","https://openalex.org/W2071973715","https://openalex.org/W2072441116","https://openalex.org/W2075303024","https://openalex.org/W2078377205","https://openalex.org/W2089034181","https://openalex.org/W2090231899","https://openalex.org/W2091020215","https://openalex.org/W2093367362","https://openalex.org/W2094043641","https://openalex.org/W2096762252","https://openalex.org/W2098997802","https://openalex.org/W2100320793","https://openalex.org/W2102385546","https://openalex.org/W2103650497","https://openalex.org/W2104484792","https://openalex.org/W2106033753","https://openalex.org/W2106345420","https://openalex.org/W2110483884","https://openalex.org/W2110727424","https://openalex.org/W2110760006","https://openalex.org/W2110760619","https://openalex.org/W2111178301","https://openalex.org/W2113279639","https://openalex.org/W2113843322","https://openalex.org/W2113853582","https://openalex.org/W2118636097","https://openalex.org/W2118752373","https://openalex.org/W2120387439","https://openalex.org/W2126236256","https://openalex.org/W2130710889","https://openalex.org/W2130981365","https://openalex.org/W2136204381","https://openalex.org/W2136913089","https://openalex.org/W2137489399","https://openalex.org/W2138898529","https://openalex.org/W2141543258","https://openalex.org/W2143764988","https://openalex.org/W2144114880","https://openalex.org/W2146033862","https://openalex.org/W2148187850","https://openalex.org/W2148323109","https://openalex.org/W2149941565","https://openalex.org/W2150825041","https://openalex.org/W2151395332","https://openalex.org/W2151907194","https://openalex.org/W2152347880","https://openalex.org/W2152973663","https://openalex.org/W2153317865","https://openalex.org/W2157129165","https://openalex.org/W2157915163","https://openalex.org/W2157972110","https://openalex.org/W2158691001","https://openalex.org/W2163970869","https://openalex.org/W2164916399","https://openalex.org/W2165105252","https://openalex.org/W2167703516","https://openalex.org/W2169802422","https://openalex.org/W2280997219","https://openalex.org/W2496549934","https://openalex.org/W2575225756","https://openalex.org/W3145650347"],"related_works":["https://openalex.org/W2307551964","https://openalex.org/W139380103","https://openalex.org/W2020224454","https://openalex.org/W2462058702","https://openalex.org/W2152347880","https://openalex.org/W1967908436","https://openalex.org/W2356230385","https://openalex.org/W2077875254","https://openalex.org/W2096762252","https://openalex.org/W2049953829"],"abstract_inverted_index":{"Mycobacterium":[0],"tuberculosis,":[1],"the":[2,13,43,54,80,93,122,134,149,156,249,309,327,335],"causative":[3],"agent":[4],"of":[5,12,32,65,90,95,125,137,142,152,158,192,231,339,342,392],"tuberculosis":[6,98],"(TB)":[7],"has":[8,50],"\\ninfected":[9],"one":[10,189],"third":[11],"world\u2019s":[14],"population":[15],"and":[16,48,118,184,243,254,273,283,295,304,352,384,386],"continues":[17],"to":[18,86,133,146,226,271,329,348],"claim":[19],"more":[20,191],"lives":[21],"\\nannually":[22],"than":[23],"any":[24],"other":[25],"infectious":[26],"disease":[27],"agent.":[28],"A":[29],"significant":[30,213],"proportion":[31],"\\nindividuals":[33],"carry":[34],"latent":[35],"TB":[36,170],"infection":[37],"(LBTI)":[38],"which":[39,75],"is":[40,354,363],"characterized":[41],"by":[42,154,197],"absence":[44],"\\nof":[45],"clinical":[46],"symptoms":[47],"it":[49,353],"been":[51],"postulated":[52],"that":[53,187,205,356,374],"tubercle":[55],"bacilli":[56],"are":[57,71,104,110,337],"in":[58,88,116,121,162,215,221,240,245,320,358,381],"a":[59,83,130,165,264,318,340],"\\ndormant-like":[60],"state":[61,94],"during":[62,237],"this":[63],"type":[64],"infection.":[66,126],"Resuscitation":[67],"promoting":[68],"factors":[69,376],"\\n(Rpfs)":[70],"cell":[72,246,296,344,367,382],"wall":[73,345,368],"hydrolases":[74],"cleave":[76],"glycosidic":[77],"bonds":[78],"within":[79],"\\npeptidoglycan":[81],"(PG),":[82],"mechanism":[84],"thought":[85],"result":[87],"reactivation":[89,113],"bacteria":[91],"\\nfrom":[92,114],"dormancy.":[96],"M.":[97,163,172,201],"encodes":[99,174],"five":[100],"rpf-like":[101,176],"homologues":[102],"\\nwhich":[103],"collectively":[105],"dispensable":[106],"for":[107,112,119,169,288,308,326,366,395],"growth":[108,216,239,383],"but":[109],"required":[111],"dormancy":[115],"vitro,":[117],"virulence":[120],"mouse":[123],"model":[124,166],"LTBI":[127],"\\nthus":[128],"poses":[129],"huge":[131],"threat":[132],"global":[135],"burden":[136],"active":[138],"disease.":[139],"The":[140,312],"purpose":[141],"\\nthis":[143],"study":[144],"was":[145],"further":[147],"investigate":[148],"biological":[150],"roles":[151],"Rpfs":[153],"assessing":[155],"\\neffects":[157],"rpf":[159,349],"gene":[160,350],"deletion":[161,185,314,351],"smegmatis,":[164],"organism":[167],"used":[168],"\\nresearch.":[171],"smegmatis":[173,202],"four":[175],"genes":[177,194],"designated":[178],"rpfA,":[179,357],"rpfB,":[180],"rpfC":[181,262],"\\nand":[182,220],"rpfE,":[183],"mutants":[186,328],"lack":[188],"or":[190,209],"these":[193,289,375],"were":[195,286],"\\nconstructed":[196],"allelic":[198],"exchange":[199],"mutagenesis.":[200],"mutant":[203,253],"strains":[204,315],"\\nlack":[206],"either":[207],"rpfA":[208,232,250,260],"rpfB":[210,251,261],"display":[211],"no":[212],"differences":[214],"both":[217],"on":[218],"solid":[219],"liquid":[222],"medium":[223],"when":[224],"compared":[225],"wild":[227],"type.":[228],"However,":[229],"loss":[230,341],"resulted":[233],"\\nin":[234,269],"bacterial":[235,343],"clumping":[236],"stationary-phase":[238],"broth":[241],"culture":[242],"\\nchanges":[244],"morphology.":[247],"Moreover;":[248],"double":[252],"its":[255],"\\nderivative":[256],"strain":[257],"lacking":[258],"rpfC,":[259],"displayed":[263,317],"ca.":[265],"2-4":[266],"log":[267],"increase":[268],"susceptibility":[270],"erythromycin":[272],"vancomycin.":[274],"Furthermore,":[275],"unusual":[276],"colonial":[277],"\\nmorphologies":[278],"with":[279,360],"reduced":[280],"serpentine":[281],"cording":[282],"smooth":[284],"peripheries":[285],"\\nobserved":[287],"multiple":[290,313],"mutants.":[291,311],"Cell":[292],"surface":[293],"defects":[294],"distortions":[297],"\\nwere":[298],"evidenced":[299],"as":[300],"wrinkle-textured,":[301],"bent":[302],"cells":[303],"polar":[305],"tip":[306],"bulges":[307],"\\nabovementioned":[310],"also":[316],"defect":[319],"\\nbiofilm":[321],"formation":[322],"revealing":[323],"an":[324,378,389],"inability":[325],"form":[330],"complex":[331],"cell-cell":[332],"\\ninteractions.":[333],"Collectively,":[334],"data":[336,372],"suggestive":[338],"integrity":[346,369],"due":[347],"proposed":[355],"combination":[359],"\\nother":[361],"genes,":[362],"largely":[364],"responsible":[365],"maintenance.":[370],"Our":[371],"\\nindicate":[373],"play":[377],"important":[379],"role":[380],"division":[385],"\\ntherefore":[387],"represent":[388],"untapped":[390],"source":[391],"novel":[393],"targets":[394],"anti-tubercular":[396],"drug":[397],"\\ndiscovery.":[398]},"counts_by_year":[],"updated_date":"2026-08-30T07:30:35.949966","created_date":"2016-06-24T00:00:00"}