{"id":"https://openalex.org/W1594582912","doi":"https://doi.org/10.11598/btb.2003.0.20.182","title":"HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS","display_name":"HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS","publication_year":2003,"publication_date":"2003-06-30","ids":{"openalex":"https://openalex.org/W1594582912","doi":"https://doi.org/10.11598/btb.2003.0.20.182","mag":"1594582912"},"language":"en","primary_location":{"id":"doi:10.11598/btb.2003.0.20.182","doi":"https://doi.org/10.11598/btb.2003.0.20.182","is_oa":true,"landing_page_url":"https://doi.org/10.11598/btb.2003.0.20.182","pdf_url":"http://journal.biotrop.org/index.php/biotropia/article/download/182/151","source":{"id":"https://openalex.org/S2764614200","display_name":"BIOTROPIA","issn_l":"0215-6334","issn":["0215-6334","1907-770X","2309-544X"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310315158","host_organization_name":"SEAMEO, BIOTROP","host_organization_lineage":["https://openalex.org/P4310315158"],"host_organization_lineage_names":["SEAMEO, BIOTROP"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIOTROPIA","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"http://journal.biotrop.org/index.php/biotropia/article/download/182/151","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5020456363","display_name":"Antonius Suwanto","orcid":"https://orcid.org/0000-0002-8765-1548","observed_orcids":["https://orcid.org/0000-0002-8765-1548"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"ANTONIUS SUWANTO","raw_affiliation_strings":["Department of Biology, Faculty of Mathematics and Sciences,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Faculty of Mathematics and Sciences,","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080977058","display_name":"Amarila Malik","orcid":"https://orcid.org/0000-0003-4899-6778","observed_orcids":["https://orcid.org/0000-0003-4899-6778"]},"institutions":[{"id":"https://openalex.org/I4405268883","display_name":"Southeast Asian Regional Centre for Tropical Biology","ror":"https://ror.org/02crwsz78","country_code":"ID","type":"facility","lineage":["https://openalex.org/I4405268883"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"AMARILA MALIK","raw_affiliation_strings":["Southeast Asian Regional Center for Tropical Biology (SEAMEO BIOTROP), Bogor,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Southeast Asian Regional Center for Tropical Biology (SEAMEO BIOTROP), Bogor,","institution_ids":["https://openalex.org/I4405268883"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109814772","display_name":"Budi Tjahjono","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I21881038","display_name":"IPB University","ror":"https://ror.org/05smgpd89","country_code":"ID","type":"education","lineage":["https://openalex.org/I21881038"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"BUDI TJAHJONO","raw_affiliation_strings":["Department of Plant Pests and Diseases, Faculty of Agriculture, Bogor Agricultural University, Bogor-16144, Indonesia: '"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Pests and Diseases, Faculty of Agriculture, Bogor Agricultural University, Bogor-16144, Indonesia: '","institution_ids":["https://openalex.org/I21881038"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111596878","display_name":"R. Harling","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405264319","display_name":"Scottish Agricultural College","ror":"https://ror.org/01jgdf008","country_code":"GB","type":"education","lineage":["https://openalex.org/I260719752","https://openalex.org/I4405264319"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"ROB HARLING","raw_affiliation_strings":["Department of Crop Science. Scottish Agricultural College, Edinburgh EH9 3JG, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Crop Science. Scottish Agricultural College, Edinburgh EH9 3JG, UK","institution_ids":["https://openalex.org/I4405264319"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"0","issue":"20","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12683","display_name":"Studies on Chitinases and Chitosanases","score":0.9570000171661377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12683","display_name":"Studies on Chitinases and Chitosanases","score":0.9570000171661377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.020800000056624413,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.012000000104308128,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chitinase","display_name":"chitinase","score":0.7870000004768372},{"id":"https://openalex.org/keywords/aeromonas-caviae","display_name":"Aeromonas caviae","score":0.9959999918937683},{"id":"https://openalex.org/keywords/pseudomonas-fluorescens","display_name":"Pseudomonas fluorescens","score":0.984000027179718},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.8529999852180481},{"id":"https://openalex.org/keywords/transcriptional-fusion","display_name":"transcriptional fusion","score":0.9599999785423279},{"id":"https://openalex.org/keywords/t7-promoter","display_name":"T7 promoter","score":0.4620000123977661},{"id":"https://openalex.org/keywords/triparental-mating","display_name":"triparental mating","score":0.7139999866485596},{"id":"https://openalex.org/keywords/chitin-degradation","display_name":"chitin degradation","score":0.9020000100135803},{"id":"https://openalex.org/keywords/fungal-antagonism","display_name":"fungal antagonism","score":0.8379999995231628},{"id":"https://openalex.org/keywords/plant-pathogenic-fungi","display_name":"plant pathogenic fungi","score":0.8849999904632568},{"id":"https://openalex.org/keywords/phyllosphere-bacteria","display_name":"phyllosphere bacteria","score":0.7960000038146973},{"id":"https://openalex.org/keywords/broad-host-range-plasmids","display_name":"broad-host-range plasmids","score":0.7839999794960022}],"concepts":[{"id":"https://openalex.org/C2776835041","wikidata":"https://www.wikidata.org/wiki/Q135292","display_name":"Pseudomonas fluorescens","level":3,"score":0.9050623774528503},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6495972871780396},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6207525134086609},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.609295129776001},{"id":"https://openalex.org/C2780778831","wikidata":"https://www.wikidata.org/wiki/Q424099","display_name":"Chitinase","level":3,"score":0.600634753704071},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5133208632469177},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.4713280498981476},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.46299275755882263},{"id":"https://openalex.org/C145640855","wikidata":"https://www.wikidata.org/wiki/Q7505713","display_name":"Shuttle vector","level":5,"score":0.43513110280036926},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4328486919403076},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.30442705750465393},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1544966697692871},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.15267330408096313}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.11598/btb.2003.0.20.182","doi":"https://doi.org/10.11598/btb.2003.0.20.182","is_oa":true,"landing_page_url":"https://doi.org/10.11598/btb.2003.0.20.182","pdf_url":"http://journal.biotrop.org/index.php/biotropia/article/download/182/151","source":{"id":"https://openalex.org/S2764614200","display_name":"BIOTROPIA","issn_l":"0215-6334","issn":["0215-6334","1907-770X","2309-544X"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310315158","host_organization_name":"SEAMEO, BIOTROP","host_organization_lineage":["https://openalex.org/P4310315158"],"host_organization_lineage_names":["SEAMEO, BIOTROP"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIOTROPIA","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:b4774f11064c4d42bee59afb696ac878","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/b4774f11064c4d42bee59afb696ac878","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biotropia: The Southeast Asian Journal of Tropical Biology, Iss 20 (2011)","raw_type":"article"},{"id":"pmh:oai:doaj.org/article:c02ad6d583c3448989a3be1957542cb5","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/c02ad6d583c3448989a3be1957542cb5","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biotropia: The Southeast Asian Journal of Tropical Biology, Vol 20, Pp 36-48 (2003)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.11598/btb.2003.0.20.182","doi":"https://doi.org/10.11598/btb.2003.0.20.182","is_oa":true,"landing_page_url":"https://doi.org/10.11598/btb.2003.0.20.182","pdf_url":"http://journal.biotrop.org/index.php/biotropia/article/download/182/151","source":{"id":"https://openalex.org/S2764614200","display_name":"BIOTROPIA","issn_l":"0215-6334","issn":["0215-6334","1907-770X","2309-544X"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310315158","host_organization_name":"SEAMEO, BIOTROP","host_organization_lineage":["https://openalex.org/P4310315158"],"host_organization_lineage_names":["SEAMEO, BIOTROP"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIOTROPIA","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321637","display_name":"Department for International Development","ror":"https://ror.org/05rf29967"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1594582912.pdf","grobid_xml":"https://content.openalex.org/works/W1594582912.grobid-xml"},"referenced_works_count":24,"referenced_works":["https://openalex.org/W189047847","https://openalex.org/W1273582181","https://openalex.org/W1547610396","https://openalex.org/W1608181111","https://openalex.org/W1756142454","https://openalex.org/W1763493835","https://openalex.org/W1863451475","https://openalex.org/W1983064515","https://openalex.org/W1987355768","https://openalex.org/W1991300031","https://openalex.org/W2011047554","https://openalex.org/W2012325090","https://openalex.org/W2025605962","https://openalex.org/W2049212373","https://openalex.org/W2054901867","https://openalex.org/W2085328625","https://openalex.org/W2092564660","https://openalex.org/W2099344381","https://openalex.org/W2109726053","https://openalex.org/W2121178227","https://openalex.org/W2126102570","https://openalex.org/W2128635872","https://openalex.org/W2164510077","https://openalex.org/W4229841461"],"related_works":["https://openalex.org/W2352370753","https://openalex.org/W2277184110","https://openalex.org/W425120505","https://openalex.org/W2384440037","https://openalex.org/W2377476444","https://openalex.org/W2337264452","https://openalex.org/W1273582181","https://openalex.org/W2038580786","https://openalex.org/W849635381","https://openalex.org/W2975889024"],"abstract_inverted_index":{"A":[0],"transcriptional":[1,45,50],"fusion":[2,46],"for":[3,86],"an":[4],"Aeromonas":[5,123],"caviae":[6],"chitinase":[7,90,125],"gene":[8,22],"was":[9,26,58,78],"constructed":[10],"under":[11],"the":[12,19,68],"control":[13],"of":[14,18,64,89],"a":[15,29,54,75,92,106],"constitutive":[16,87],"promoter":[17],"kanaraycin":[20],"resistance":[21],"(PKmR).":[23],"The":[24],"construct":[25],"inserted":[27],"into":[28],"medium":[30],"copy":[31],"number":[32],"broad":[33],"host":[34],"range":[35],"plasmid":[36,41,56],"vector":[37],"to":[38],"yield":[39],"recombinant":[40,55,69],"pAM340,":[42],"which":[43],"harbored":[44],"PKmR-":[47],"chi.":[48],"Another":[49],"fusion,":[51],"Ptac-chi,":[52],"in":[53],"pAM630,":[57],"conducted":[59],"as":[60],"comparison.":[61],"Triparental":[62],"mating":[63],"E.":[65],"coli":[66,103],"carrying":[67],"plasmids":[70],"with":[71],"Pseudomotws":[72],"fluorescens":[73,81,129],"5100,":[74],"phyllosphere":[76],"bacterium,":[77],"performed.":[79],"Pseudomonas":[80],"5100":[82],"exconjugants":[83],"were":[84],"examined":[85],"expression":[88],"employing":[91],"spectrophotometric":[93],"assay;":[94],"they":[95],"showed":[96],"stronger":[97],"chitin":[98],"degradation":[99],"activity":[100],"than":[101],"Escherichia":[102],"transformants.":[104],"Using":[105],"fungal":[107],"antagonism":[108],"plate":[109],"assay,":[110],"this":[111],"chitinolytic":[112],"P.":[113],"fluorescens,":[114],"however,":[115],"could":[116],"not":[117],"inhibit":[118],"selected":[119],"phytopathogenic":[120],"fungi.":[121],"Keywords:":[122],"caviae/":[124],"gene/transcriptional":[126],"fusion/PKm'V":[127],"Vtac-chilPseudomonas":[128]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}