{"id":"https://openalex.org/W1594038744","doi":"https://doi.org/10.1016/s0021-9258(18)55029-8","title":"Arginine-glycine-aspartic acid binding leading to molecular stabilization between integrin alpha v beta 3 and its ligand.","display_name":"Arginine-glycine-aspartic acid binding leading to molecular stabilization between integrin alpha v beta 3 and its ligand.","publication_year":1991,"publication_date":"1991-10-01","ids":{"openalex":"https://openalex.org/W1594038744","doi":"https://doi.org/10.1016/s0021-9258(18)55029-8","mag":"1594038744","pmid":"https://pubmed.ncbi.nlm.nih.gov/1717468"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)55029-8","doi":"https://doi.org/10.1016/s0021-9258(18)55029-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)55029-8","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)55029-8","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049439690","display_name":"Robert A. Orlando","orcid":"https://orcid.org/0000-0001-5445-0371","observed_orcids":["https://orcid.org/0000-0001-5445-0371"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]},{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R.A. Orlando","raw_affiliation_strings":["Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093","From the Department of Immunology, The Scripps Research Institute, La J o b , California 92037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093","institution_ids":["https://openalex.org/I36258959"]},{"raw_affiliation_string":"From the Department of Immunology, The Scripps Research Institute, La J o b , California 92037","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089796560","display_name":"David A. Cheresh","orcid":"https://orcid.org/0000-0002-7193-8595","observed_orcids":["https://orcid.org/0000-0002-7193-8595"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]},{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D.A. Cheresh","raw_affiliation_strings":["Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093","From the Department of Immunology, The Scripps Research Institute, La J o b , California 92037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093","institution_ids":["https://openalex.org/I36258959"]},{"raw_affiliation_string":"From the Department of Immunology, The Scripps Research Institute, La J o b , California 92037","institution_ids":["https://openalex.org/I123431417"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.1403,"has_fulltext":false,"cited_by_count":103,"citation_normalized_percentile":{"value":0.86909528,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"266","issue":"29","first_page":"19543","last_page":"19550"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/integrin-alphavbeta3","display_name":"integrin \u03b1v\u03b23","score":0.9919999837875366},{"id":"https://openalex.org/keywords/vitronectin","display_name":"vitronectin","score":0.8849999904632568},{"id":"https://openalex.org/keywords/rgd-peptide","display_name":"RGD peptide","score":0.8209999799728394},{"id":"https://openalex.org/keywords/ligand-binding","display_name":"ligand binding","score":0.6539999842643738},{"id":"https://openalex.org/keywords/cell-adhesion","display_name":"cell adhesion","score":0.8420000076293945},{"id":"https://openalex.org/keywords/actin-cytoskeleton","display_name":"actin cytoskeleton","score":0.7250000238418579},{"id":"https://openalex.org/keywords/fibronectin","display_name":"fibronectin","score":0.8299999833106995},{"id":"https://openalex.org/keywords/glutaraldehyde-crosslinking","display_name":"glutaraldehyde crosslinking","score":0.7829999923706055},{"id":"https://openalex.org/keywords/receptor-conformational-change","display_name":"receptor conformational change","score":0.8069999814033508},{"id":"https://openalex.org/keywords/dissociation-constant","display_name":"dissociation constant","score":0.5860000252723694}],"concepts":[{"id":"https://openalex.org/C164144092","wikidata":"https://www.wikidata.org/wiki/Q6587033","display_name":"Vitronectin","level":4,"score":0.9405609369277954},{"id":"https://openalex.org/C2777141677","wikidata":"https://www.wikidata.org/wiki/Q50254584","display_name":"Alpha-v beta-3","level":5,"score":0.7906757593154907},{"id":"https://openalex.org/C195687474","wikidata":"https://www.wikidata.org/wiki/Q409715","display_name":"Integrin","level":3,"score":0.7017379999160767},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.48819905519485474},{"id":"https://openalex.org/C83730767","wikidata":"https://www.wikidata.org/wiki/Q24778884","display_name":"G alpha subunit","level":4,"score":0.45806884765625},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4530978500843048},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.4407445192337036},{"id":"https://openalex.org/C2993400109","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Actin cytoskeleton","level":4,"score":0.43845972418785095},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.43087100982666016},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.43074122071266174},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.369897723197937},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3444301187992096},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.276748389005661},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.1846635341644287},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D003571","descriptor_name":"Cytochalasin B","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005976","descriptor_name":"Glutaral","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D016023","descriptor_name":"Integrins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019096","descriptor_name":"Vitronectin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)55029-8","doi":"https://doi.org/10.1016/s0021-9258(18)55029-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)55029-8","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:1717468","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1717468","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)55029-8","doi":"https://doi.org/10.1016/s0021-9258(18)55029-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)55029-8","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1648785187","display_name":null,"funder_award_id":"CA-50286","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G2003983467","display_name":null,"funder_award_id":"CA-45726","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G243309107","display_name":"Integrin alpha v beta 3 promotes resistance to EGF receptor inhibitors","funder_award_id":"R01CA045726","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6667797187","display_name":"Immunology Training","funder_award_id":"T32AI007244","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G8539379466","display_name":null,"funder_award_id":"T32 AI 07244-08","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":41,"referenced_works":["https://openalex.org/W1141074930","https://openalex.org/W1234014522","https://openalex.org/W1520666812","https://openalex.org/W1524285426","https://openalex.org/W1524805668","https://openalex.org/W1577857859","https://openalex.org/W1594881395","https://openalex.org/W1595132971","https://openalex.org/W1901030926","https://openalex.org/W1964394713","https://openalex.org/W1968654001","https://openalex.org/W1979120993","https://openalex.org/W1987568997","https://openalex.org/W1990387345","https://openalex.org/W1991011809","https://openalex.org/W1996789033","https://openalex.org/W1999794993","https://openalex.org/W2008796478","https://openalex.org/W2013381560","https://openalex.org/W2037550327","https://openalex.org/W2044806537","https://openalex.org/W2059437136","https://openalex.org/W2066279385","https://openalex.org/W2069294982","https://openalex.org/W2071676754","https://openalex.org/W2079312655","https://openalex.org/W2088546959","https://openalex.org/W2093366573","https://openalex.org/W2103725270","https://openalex.org/W2109505295","https://openalex.org/W2112093320","https://openalex.org/W2131991335","https://openalex.org/W2139666587","https://openalex.org/W2162700552","https://openalex.org/W2167071561","https://openalex.org/W2167983317","https://openalex.org/W2169947138","https://openalex.org/W2406745790","https://openalex.org/W2413462612","https://openalex.org/W2415265619","https://openalex.org/W4252552832"],"related_works":["https://openalex.org/W1571638637","https://openalex.org/W1999794993","https://openalex.org/W4406986852","https://openalex.org/W2042928146","https://openalex.org/W2051092663","https://openalex.org/W1984452582","https://openalex.org/W1539115017","https://openalex.org/W1595132971","https://openalex.org/W140814971","https://openalex.org/W1974342547"],"abstract_inverted_index":{"Cell":[0],"adhesion":[1],"is":[2,96,158],"characterized":[3],"by":[4,11,67],"an":[5],"integrin-mediated":[6],"ligand":[7,70,204],"binding":[8,119,205,229],"event":[9],"followed":[10],"reorganization":[12],"of":[13,29,48,98,106,164,172,217,232],"the":[14,27,46,99,103,203,210,215,233],"actin-cytoskeleton":[15],"leading":[16,236],"to":[17,39,51,60,74,87,115,140,183,202,237],"cell":[18,37,58,85],"spreading":[19],"and/or":[20],"migration.":[21],"In":[22],"this":[23,107],"report":[24],"we":[25],"examine":[26],"role":[28],"integrin":[30,224],"alpha":[31,81,109,135,173,225],"v":[32,82,110,136,174,226],"beta":[33,83,111,137,175,227],"3":[34,112,138,176],"in":[35,45,90,126,144,185,209,214],"mediating":[36],"attachment":[38,59,86],"vitronectin":[40,75,88,141,154,182],"or":[41,142,196,220],"a":[42,61,91,127,145,149,162,186,238],"RGD-containing":[43,62,150],"peptide":[44,63,151],"presence":[47],"cytochalasin":[49],"B":[50],"prevent":[52],"actin":[53],"polymerization.":[54],"Under":[55],"these":[56],"conditions":[57],"can":[64],"be":[65],"dissociated":[66],"excess":[68],"soluble":[69],"whereas":[71,148],"cells":[72],"attached":[73],"cannot.":[76],"These":[77],"results":[78,89],"suggest":[79],"that":[80,95,134,192],"3-mediated":[84],"highly":[92,239],"stabilized":[93,240],"interaction":[94,108],"independent":[97],"actin-cytoskeleton.":[100],"To":[101],"investigate":[102],"molecular":[104],"nature":[105],"was":[113],"purified":[114],"homogeneity,":[116],"and":[117,188],"its":[118],"properties":[120],"toward":[121],"various":[122],"ligands":[123],"were":[124],"measured":[125],"solid-phase":[128],"receptor":[129,193],"assay.":[130],"The":[131],"data":[132],"indicate":[133],"binds":[139,155],"fibronectin":[143],"nondissociable":[146],"manner":[147],"derived":[152],"from":[153],"specifically":[156],"but":[157],"completely":[159],"dissociable":[160,189],"with":[161,177],"Kd":[163],"9.4":[165],"x":[166],"10(-7)":[167],"M.":[168],"Moreover,":[169],"chemical":[170],"modification":[171],"limited":[178],"glutaraldehyde":[179],"treatment":[180],"allowed":[181],"bind":[184],"RGD-dependent":[187],"manner,":[190],"suggesting":[191],"conformational":[194],"changes":[195],"specific":[197],"amino":[198],"acid":[199],"residues":[200],"proximal":[201],"site(s)":[206],"are":[207],"involved":[208],"stabilization":[211],"event.":[212],"Thus,":[213],"absence":[216],"cytoskeletal":[218],"proteins":[219],"other":[221],"cellular":[222],"components,":[223],"3-ligand":[228],"involves":[230],"recognition":[231],"RGD":[234],"sequence":[235],"protein-protein":[241],"association.":[242]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}