{"id":"https://openalex.org/W15940141","doi":"https://doi.org/10.1007/978-1-60761-362-6_29","title":"Activation of Human NK Cells by Malaria-Infected Red Blood Cells","display_name":"Activation of Human NK Cells by Malaria-Infected Red Blood Cells","publication_year":2009,"publication_date":"2009-11-09","ids":{"openalex":"https://openalex.org/W15940141","doi":"https://doi.org/10.1007/978-1-60761-362-6_29","mag":"15940141","pmid":"https://pubmed.ncbi.nlm.nih.gov/20033658"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-60761-362-6_29","doi":"https://doi.org/10.1007/978-1-60761-362-6_29","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60761-362-6_29","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2487077681","display_name":"Natural Killer Cell Protocols"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083361833","display_name":"Amir Horowitz","orcid":"https://orcid.org/0000-0003-3100-2810","observed_orcids":["https://orcid.org/0000-0003-3100-2810","https://orcid.org/0000-0003-4602-1440"]},"institutions":[{"id":"https://openalex.org/I4210089966","display_name":"London School of Hygiene & Tropical Medicine","ror":"https://ror.org/00a0jsq62","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I4210089966"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Amir Horowitz","raw_affiliation_strings":["Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK","London School of Hygiene & Tropical Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK","institution_ids":["https://openalex.org/I4210089966"]},{"raw_affiliation_string":"London School of Hygiene & Tropical Medicine","institution_ids":["https://openalex.org/I4210089966"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5017348677","display_name":"Eleanor M. Riley","orcid":"https://orcid.org/0000-0003-3447-3570","observed_orcids":["https://orcid.org/0000-0003-3447-3570"]},"institutions":[{"id":"https://openalex.org/I4210089966","display_name":"London School of Hygiene & Tropical Medicine","ror":"https://ror.org/00a0jsq62","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I4210089966"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Eleanor M. Riley","raw_affiliation_strings":["Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK","London School of Hygiene & Tropical Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK","institution_ids":["https://openalex.org/I4210089966"]},{"raw_affiliation_string":"London School of Hygiene & Tropical Medicine","institution_ids":["https://openalex.org/I4210089966"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210089966"],"apc_list":null,"apc_paid":null,"fwci":2.7053,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.89184397,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"612","issue":null,"first_page":"429","last_page":"446"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11020","display_name":"Immune Cell Function and Interaction","score":0.9086999893188477,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11020","display_name":"Immune Cell Function and Interaction","score":0.9086999893188477,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.08760000020265579,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11660","display_name":"Blood groups and transfusion","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nk-cell-activation","display_name":"NK cell activation","score":0.8259999752044678},{"id":"https://openalex.org/keywords/malaria-infected-red-blood-cells","display_name":"malaria-infected red blood cells","score":0.8930000066757202},{"id":"https://openalex.org/keywords/plasmodium-falciparum","display_name":"Plasmodium falciparum","score":0.9929999709129333},{"id":"https://openalex.org/keywords/cd69","display_name":"CD69","score":0.9300000071525574},{"id":"https://openalex.org/keywords/interferon-gamma","display_name":"interferon-gamma","score":0.9229999780654907},{"id":"https://openalex.org/keywords/peripheral-blood-mononuclear-cells","display_name":"peripheral blood mononuclear cells","score":0.902999997138977},{"id":"https://openalex.org/keywords/parasite-propagation","display_name":"parasite propagation","score":0.7160000205039978},{"id":"https://openalex.org/keywords/parasite-isolation","display_name":"parasite isolation","score":0.3269999921321869}],"concepts":[{"id":"https://openalex.org/C137061746","wikidata":"https://www.wikidata.org/wiki/Q736033","display_name":"Peripheral blood mononuclear cell","level":3,"score":0.7870343923568726},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.7173173427581787},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.6076377034187317},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.5800858736038208},{"id":"https://openalex.org/C2778371730","wikidata":"https://www.wikidata.org/wiki/Q311383","display_name":"Plasmodium falciparum","level":3,"score":0.5149806141853333},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49847888946533203},{"id":"https://openalex.org/C132946820","wikidata":"https://www.wikidata.org/wiki/Q2509459","display_name":"Differential centrifugation","level":2,"score":0.4747386574745178},{"id":"https://openalex.org/C176324957","wikidata":"https://www.wikidata.org/wiki/Q21109696","display_name":"CD69","level":5,"score":0.4455582797527313},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4358980059623718},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.42818483710289},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.4118937849998474},{"id":"https://openalex.org/C2778048844","wikidata":"https://www.wikidata.org/wiki/Q12156","display_name":"Malaria","level":2,"score":0.37025630474090576},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3476216793060303},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.18702447414398193},{"id":"https://openalex.org/C79484868","wikidata":"https://www.wikidata.org/wiki/Q5971673","display_name":"IL-2 receptor","level":4,"score":0.13373571634292603},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08692699670791626},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004912","descriptor_name":"Erythrocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D004912","descriptor_name":"Erythrocytes","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007371","descriptor_name":"Interferon-gamma","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D007694","descriptor_name":"Killer Cells, Natural","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D007694","descriptor_name":"Killer Cells, Natural","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D007694","descriptor_name":"Killer Cells, Natural","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008280","descriptor_name":"Magnetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010963","descriptor_name":"Plasmodium falciparum","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015703","descriptor_name":"Antigens, CD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015854","descriptor_name":"Up-Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D042541","descriptor_name":"Intracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1007/978-1-60761-362-6_29","doi":"https://doi.org/10.1007/978-1-60761-362-6_29","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60761-362-6_29","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:20033658","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20033658","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:researchonline.lshtm.ac.uk:19871","doi":null,"is_oa":false,"landing_page_url":"http://researchonline.lshtm.ac.uk/id/eprint/19871/","pdf_url":null,"source":{"id":"https://openalex.org/S4306402527","display_name":"LSHTM Research Online (London School of Hygiene and Tropical Medicine)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210089966","host_organization_name":"London School of Hygiene & Tropical Medicine","host_organization_lineage":["https://openalex.org/I4210089966"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1988748224","https://openalex.org/W2010002974","https://openalex.org/W2012313438","https://openalex.org/W2081670280","https://openalex.org/W2083547256","https://openalex.org/W2097210912","https://openalex.org/W2103214447","https://openalex.org/W2108939559","https://openalex.org/W2114807822","https://openalex.org/W2122047942","https://openalex.org/W2130467976","https://openalex.org/W2132282141","https://openalex.org/W2140362827","https://openalex.org/W2144085552","https://openalex.org/W2146652141","https://openalex.org/W2155326193","https://openalex.org/W2157657263","https://openalex.org/W2159164435","https://openalex.org/W2314524827"],"related_works":["https://openalex.org/W1870638978","https://openalex.org/W2157657263","https://openalex.org/W1945764847","https://openalex.org/W2122047942","https://openalex.org/W1815771787","https://openalex.org/W2159164435","https://openalex.org/W2070640683","https://openalex.org/W2587203650","https://openalex.org/W2894898302","https://openalex.org/W2141047461"],"abstract_inverted_index":{"This":[0],"chapter":[1],"describes":[2],"a":[3],"protocol":[4],"to":[5],"assess":[6],"activation":[7],"of":[8,32,36,48,53,67,74,81],"human":[9],"NK":[10],"cells":[11,57],"following":[12],"in":[13,43,64],"vitro":[14,44,65],"stimulation":[15],"with":[16],"malaria-infected":[17],"red":[18],"blood":[19,55,60],"cells.":[20],"Activation":[21],"is":[22],"assessed":[23],"by":[24,61,76],"flow":[25,77],"cytometry,":[26],"staining":[27,71],"for":[28,42,70],"cell":[29],"surface":[30],"expression":[31],"CD69":[33],"and":[34,46,69,72],"accumulation":[35],"intracellular":[37],"IFN-gamma.":[38],"Procedures":[39],"are":[40,85],"described":[41],"propagation":[45],"purification":[47],"Plasmodium":[49],"falciparum":[50],"parasites,":[51],"separation":[52],"peripheral":[54],"mononuclear":[56],"from":[58],"heparinised":[59],"density":[62],"centrifugation,":[63],"culture":[66],"PBMC":[68,75],"analysis":[73],"cytometry.":[78],"Some":[79],"examples":[80],"typical":[82],"FACS":[83],"plots":[84],"shown.":[86]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}