{"id":"https://openalex.org/W1592638705","doi":null,"title":"The prevention of heparanase expression in endothelial cells injured by high glucose","display_name":"The prevention of heparanase expression in endothelial cells injured by high glucose","publication_year":2005,"publication_date":"2005-04-25","ids":{"openalex":"https://openalex.org/W1592638705","mag":"1592638705"},"language":"en","primary_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-04282005-160750","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-04282005-160750","pdf_url":"https://harvest.usask.ca/bitstreams/57c3f9e3-6ca9-4297-ac9c-4aaaa543473b/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://harvest.usask.ca/bitstreams/57c3f9e3-6ca9-4297-ac9c-4aaaa543473b/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084627867","display_name":"Ju Ying Han","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ju Ying Han","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5084627867"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11142","display_name":"Advanced Glycation End Products research","score":0.009200000204145908,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.002899999963119626,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heparanase","display_name":"heparanase","score":0.9829999804496765},{"id":"https://openalex.org/keywords/endothelial-injury","display_name":"endothelial injury","score":0.800000011920929},{"id":"https://openalex.org/keywords/hyperglycemia","display_name":"hyperglycemia","score":0.953000009059906},{"id":"https://openalex.org/keywords/diabetes-mellitus","display_name":"diabetes mellitus","score":0.8289999961853027},{"id":"https://openalex.org/keywords/endothelial-dysfunction","display_name":"endothelial dysfunction","score":0.8669999837875366},{"id":"https://openalex.org/keywords/heparinase-i","display_name":"heparinase I","score":0.6579999923706055},{"id":"https://openalex.org/keywords/porcine-aortic-endothelial-cells","display_name":"porcine aortic endothelial cells","score":0.9049999713897705},{"id":"https://openalex.org/keywords/heparin","display_name":"heparin","score":0.7059999704360962},{"id":"https://openalex.org/keywords/insulin","display_name":"insulin","score":0.9079999923706055},{"id":"https://openalex.org/keywords/basic-fibroblast-growth-factor","display_name":"basic fibroblast growth factor","score":0.996999979019165},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.7310000061988831},{"id":"https://openalex.org/keywords/osmolarity","display_name":"osmolarity","score":0.7590000033378601},{"id":"https://openalex.org/keywords/diabetic-vascular-complications","display_name":"diabetic vascular complications","score":0.6010000109672546}],"concepts":[{"id":"https://openalex.org/C2777478136","wikidata":"https://www.wikidata.org/wiki/Q909646","display_name":"Heparanase","level":4,"score":0.5452341437339783},{"id":"https://openalex.org/C90559484","wikidata":"https://www.wikidata.org/wiki/Q778379","display_name":"Expression (computer science)","level":2,"score":0.4831099510192871},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.43567103147506714},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3213745355606079},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.31266915798187256},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3106120824813843},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.21468806266784668},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.213700532913208},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18351632356643677},{"id":"https://openalex.org/C2778041096","wikidata":"https://www.wikidata.org/wiki/Q424668","display_name":"Heparan sulfate","level":3,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:ecommons.usask.ca:10388/etd-04282005-160750","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-04282005-160750","pdf_url":"https://harvest.usask.ca/bitstreams/57c3f9e3-6ca9-4297-ac9c-4aaaa543473b/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},{"id":"pmh:oai:harvest.usask.ca:10388/etd-04282005-160750","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10388/etd-04282005-160750","pdf_url":null,"source":{"id":"https://openalex.org/S7407063267","display_name":"HARVEST (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:1592638705","doi":null,"is_oa":false,"landing_page_url":"https://harvest.usask.ca/bitstream/10388/etd-04282005-160750/1/jy_han.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-04282005-160750","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-04282005-160750","pdf_url":"https://harvest.usask.ca/bitstreams/57c3f9e3-6ca9-4297-ac9c-4aaaa543473b/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"sustainable_development_goals":[{"score":0.98580002784729,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1592638705.pdf","grobid_xml":"https://content.openalex.org/works/W1592638705.grobid-xml"},"referenced_works_count":185,"referenced_works":["https://openalex.org/W133465157","https://openalex.org/W158010217","https://openalex.org/W190833853","https://openalex.org/W206526553","https://openalex.org/W636291446","https://openalex.org/W1491748226","https://openalex.org/W1526312778","https://openalex.org/W1533359222","https://openalex.org/W1545069508","https://openalex.org/W1546961183","https://openalex.org/W1575121183","https://openalex.org/W1595861603","https://openalex.org/W1599174829","https://openalex.org/W1640074353","https://openalex.org/W1766167996","https://openalex.org/W1785684379","https://openalex.org/W1830747593","https://openalex.org/W1869562643","https://openalex.org/W1902029503","https://openalex.org/W1913314522","https://openalex.org/W1959298931","https://openalex.org/W1964360262","https://openalex.org/W1965044378","https://openalex.org/W1968412767","https://openalex.org/W1969181249","https://openalex.org/W1972220693","https://openalex.org/W1972580340","https://openalex.org/W1974873948","https://openalex.org/W1975370922","https://openalex.org/W1975974235","https://openalex.org/W1978001969","https://openalex.org/W1978444547","https://openalex.org/W1979175683","https://openalex.org/W1982371349","https://openalex.org/W1982811953","https://openalex.org/W1983693801","https://openalex.org/W1984011732","https://openalex.org/W1986227565","https://openalex.org/W1986443233","https://openalex.org/W1991292863","https://openalex.org/W1992327323","https://openalex.org/W1993165881","https://openalex.org/W1993370568","https://openalex.org/W1995343667","https://openalex.org/W1996424575","https://openalex.org/W1996428478","https://openalex.org/W1996948465","https://openalex.org/W1997226913","https://openalex.org/W2001340598","https://openalex.org/W2001513964","https://openalex.org/W2001564318","https://openalex.org/W2003225199","https://openalex.org/W2006012802","https://openalex.org/W2008684260","https://openalex.org/W2008980811","https://openalex.org/W2009030155","https://openalex.org/W2010433459","https://openalex.org/W2012498125","https://openalex.org/W2013203608","https://openalex.org/W2013858460","https://openalex.org/W2016883388","https://openalex.org/W2017554243","https://openalex.org/W2019074842","https://openalex.org/W2020615342","https://openalex.org/W2023970316","https://openalex.org/W2026622673","https://openalex.org/W2026623169","https://openalex.org/W2026828445","https://openalex.org/W2026840311","https://openalex.org/W2026858231","https://openalex.org/W2027789202","https://openalex.org/W2028042013","https://openalex.org/W2033269028","https://openalex.org/W2033589357","https://openalex.org/W2035051327","https://openalex.org/W2035248906","https://openalex.org/W2040187493","https://openalex.org/W2040644761","https://openalex.org/W2044437167","https://openalex.org/W2045098684","https://openalex.org/W2045138089","https://openalex.org/W2045344587","https://openalex.org/W2045746478","https://openalex.org/W2046690057","https://openalex.org/W2046944434","https://openalex.org/W2051625732","https://openalex.org/W2054709772","https://openalex.org/W2054965394","https://openalex.org/W2055114143","https://openalex.org/W2055228437","https://openalex.org/W2055308270","https://openalex.org/W2056908901","https://openalex.org/W2057611314","https://openalex.org/W2057691724","https://openalex.org/W2058116467","https://openalex.org/W2059418292","https://openalex.org/W2059614240","https://openalex.org/W2060260000","https://openalex.org/W2060382299","https://openalex.org/W2064041280","https://openalex.org/W2064098261","https://openalex.org/W2064689833","https://openalex.org/W2065697637","https://openalex.org/W2068909924","https://openalex.org/W2069830389","https://openalex.org/W2070669362","https://openalex.org/W2072420379","https://openalex.org/W2072437489","https://openalex.org/W2073069882","https://openalex.org/W2073485208","https://openalex.org/W2073713671","https://openalex.org/W2074338808","https://openalex.org/W2076100222","https://openalex.org/W2076186944","https://openalex.org/W2076693232","https://openalex.org/W2077400092","https://openalex.org/W2078083049","https://openalex.org/W2081045088","https://openalex.org/W2082091147","https://openalex.org/W2082142266","https://openalex.org/W2082293881","https://openalex.org/W2083074809","https://openalex.org/W2085838621","https://openalex.org/W2086169454","https://openalex.org/W2086962905","https://openalex.org/W2089944271","https://openalex.org/W2090851364","https://openalex.org/W2091678536","https://openalex.org/W2091857312","https://openalex.org/W2092889119","https://openalex.org/W2094642452","https://openalex.org/W2100959766","https://openalex.org/W2101651570","https://openalex.org/W2103295047","https://openalex.org/W2104380573","https://openalex.org/W2105301755","https://openalex.org/W2106767413","https://openalex.org/W2113169270","https://openalex.org/W2113169712","https://openalex.org/W2117773410","https://openalex.org/W2123719513","https://openalex.org/W2126254515","https://openalex.org/W2127878111","https://openalex.org/W2132001104","https://openalex.org/W2135845349","https://openalex.org/W2137178544","https://openalex.org/W2140379022","https://openalex.org/W2146344567","https://openalex.org/W2146559746","https://openalex.org/W2147274452","https://openalex.org/W2147817661","https://openalex.org/W2150912407","https://openalex.org/W2151084936","https://openalex.org/W2152127342","https://openalex.org/W2153876718","https://openalex.org/W2155456675","https://openalex.org/W2156592815","https://openalex.org/W2156764890","https://openalex.org/W2158968180","https://openalex.org/W2162766735","https://openalex.org/W2169829977","https://openalex.org/W2170013186","https://openalex.org/W2180209555","https://openalex.org/W2182260120","https://openalex.org/W2183354609","https://openalex.org/W2199620244","https://openalex.org/W2204866939","https://openalex.org/W2240432631","https://openalex.org/W2324588265","https://openalex.org/W2335631045","https://openalex.org/W2337133379","https://openalex.org/W2337738945","https://openalex.org/W2402216906","https://openalex.org/W2409147391","https://openalex.org/W2409372988","https://openalex.org/W2414981517","https://openalex.org/W2416567359","https://openalex.org/W2417813337","https://openalex.org/W2431311901","https://openalex.org/W2438602659","https://openalex.org/W2471811005","https://openalex.org/W2477665208","https://openalex.org/W2975205638","https://openalex.org/W3122152400","https://openalex.org/W4299952154"],"related_works":["https://openalex.org/W134087847","https://openalex.org/W2138337378","https://openalex.org/W1942642077","https://openalex.org/W2148662334","https://openalex.org/W2054795317","https://openalex.org/W4232362871","https://openalex.org/W2996117528","https://openalex.org/W1865789674","https://openalex.org/W1964913827","https://openalex.org/W2057552014"],"abstract_inverted_index":{"Vascular":[0],"complications,":[1],"in":[2,5,11,14,26,60,78,112,166,231,309,338,344,361,381,401,450,482],"microvessels":[3],"resulting":[4,13,449],"nephropathy,":[6],"retinopathy":[7],"and":[8,10,24,77,110,118,130,155,226,320,342,353,357,379,395,440,454,487,528],"neuropathy":[9],"macrovessels":[12],"atherosclerosis":[15],"caused":[16,246,493],"by":[17,41,150,189,207,212,295,301,433,494,511],"hyperglycemia":[18,42,117,129,422,434],"contribute":[19],"greatly":[20],"to":[21,38,44,86,93,101,138,179,238,249,281,325,470,480,532],"the":[22,30,32,49,66,74,79,218,390,445,459,471,520],"morbidity":[23],"mortality":[25],"diabetes":[27,527],"mellitus.":[28],"In":[29,183],"vasculature,":[31],"endothelial":[33,61,108,121,143,428,534],"cells":[34,144,402,457,490],"(ECs)":[35],"are":[36,56],"first":[37],"be":[39,87,468],"damaged":[40],"due":[43,479],"their":[45],"unique":[46],"location":[47],"as":[48],"inner":[50],"lining":[51],"of":[52,68,88,125,133,220,392,427,447,456,464,473,522],"all":[53],"vessels.":[54],"There":[55],"several":[57],"mechanisms":[58],"involved":[59],"injury":[62,109,147,243,289,439,492,524],"or":[63,236,244,257,290,304,330,367,407,414,497],"dysfunction,":[64],"however,":[65],"degradation":[67,446],"heparan":[69,95],"sulfate":[70,96],"proteoglycan":[71],"(HSPG)":[72],"on":[73],"cell":[75,153,191,318,340],"surface":[76],"extra":[80],"cellular":[81],"matrix":[82],"(ECM)":[83],"is":[84,91,105,164,423,435],"considered":[85],"importance.":[89],"Heparanase":[90,355,430],"believed":[92],"degrade":[94],"(HS).":[97],"Our":[98],"objectives":[99],"were":[100,136,228,262],"determine":[102,115,161,239,282],"if":[103,116,162,240,283],"heparanase":[104,119,163,181,185,224,292,376,393,465,507],"responsible":[106,436],"for":[107,437],"dysfunction":[111,442],"diabetes.":[113],"To":[114,160,215],"cause":[120,426],"injury,":[122,223],"high":[123,167,221,250,255,296,302,365,382,405,495,512],"concentrations":[124],"glucose":[126,168,222,251,256,303,329,366,383,406,496],"(30mM),":[127],"mimicking":[128],"optimal":[131],"doses":[132],"heparinase":[134,258,305,331,408,498],"I":[135,259,306,332,409],"used":[137,178],"treat":[139],"cultured":[140,197],"porcine":[141],"aortic":[142],"(PAECs).":[145],"Cell":[146],"was":[148,177,187,205,359],"measured":[149,188,230],"determining":[151],"live":[152,317,339],"number":[154,319,341],"lactate":[156],"dehydrogenase":[157],"(LDH)":[158],"release.":[159],"expressed":[165,360],"treated":[169,233,260,264,333,348,363,384,403],"PAECs,":[170],"reverse":[171],"transcriptase":[172],"polymerase":[173],"chain":[174],"reaction":[175],"(RT-PCR)":[176],"amplify":[180],"mRNA.":[182],"addition,":[184],"activity":[186,227,358,380],"incubating":[190],"lysates":[192],"with":[193,234,265,349,364,404,412,526],"35S-labelled":[194],"ECM":[195],"from":[196,288,458,491],"bovine":[198],"corneal":[199],"ECs,":[200],"where":[201],"released":[202],"radioactive":[203],"HS":[204],"analyzed":[206],"Sepharose":[208],"gel":[209],"filtration":[210],"followed":[211],"\u00e2-scintillation":[213],"counting.":[214],"help":[216],"understand":[217],"mechanism":[219,463],"mRNA":[225,356,377,394,508],"also":[229,263],"PAECs":[232,261,299,362],"H2O2":[235],"mannitol":[237],"free":[241,311],"radical":[242],"osmolarity":[245],"effects":[247],"similar":[248],"treatment.":[252],"As":[253],"well,":[254],"heparin":[266,350],"(0.5":[267],"\u00ecg/ml)":[268],"and/or":[269,273,351,370,485,501],"insulin":[270,352,413,486,502],"(1":[271],"U/ml)":[272],"basic":[274],"fibroblast":[275],"growth":[276],"factor":[277],"(bFGF,":[278],"1":[279],"ng/ml)":[280],"these":[284,417],"compounds":[285],"protected":[286],"ECs":[287,334],"inhibited":[291],"expression":[293,378],"induced":[294,432,510],"glucose.":[297,513],"p*":[298],"injured":[300],"(0.3":[307],"U/ml":[308],"serum":[310],"medium)":[312],"showed":[313,335,398],"a":[314,424],"significantly":[315],"decreased":[316],"increased":[321,451],"LDH":[322,345],"release":[323,346],"compared":[324],"control":[326],"cells.":[327],"High":[328],"an":[336],"increase":[337],"decrease":[343],"when":[347,410],"bFGF.":[354],"H2O2.":[368],"Heparin":[369,484,500],"insulin,":[371],"but":[372,477,503],"not":[373,388,478,504],"bFGF":[374,397,488,505],"prevented":[375],"PAECs.":[385],"Mannitol":[386],"did":[387],"induce":[389],"upregulation":[391,466,509],"activity.":[396],"variable":[399],"protection":[400],"combined":[411],"heparin.":[415],"From":[416],"results":[418],"we":[419],"conclude":[420],"that":[421],"main":[425],"injury.":[429,535],"production":[431],"EC":[438],"vascular":[441,452,523],"likely":[443],"through":[444],"HS,":[448],"permeability":[453],"detachment":[455],"basement":[460],"membrane.":[461],"The":[462],"may":[467],"related":[469],"formation":[472],"reactive":[474],"oxygen":[475],"species,":[476],"changes":[481],"osmolarity.":[483],"protect":[489],"I.":[499],"inhibit":[506],"This":[514],"study":[515],"provides":[516],"new":[517],"insight":[518],"into":[519],"causes":[521],"associated":[525],"suggests":[529],"possible":[530],"treatments":[531],"reduce":[533]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}