{"id":"https://openalex.org/W1592452603","doi":"https://doi.org/10.1016/s0021-9258(17)43303-5","title":"Insulin-like growth factor II receptors. Molecular radius and molecular weight determination using quantitative polyacrylamide gel electrophoresis.","display_name":"Insulin-like growth factor II receptors. Molecular radius and molecular weight determination using quantitative polyacrylamide gel electrophoresis.","publication_year":1984,"publication_date":"1984-03-01","ids":{"openalex":"https://openalex.org/W1592452603","doi":"https://doi.org/10.1016/s0021-9258(17)43303-5","mag":"1592452603","pmid":"https://pubmed.ncbi.nlm.nih.gov/6321506"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(17)43303-5","doi":"https://doi.org/10.1016/s0021-9258(17)43303-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(17)43303-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(17)43303-5","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"C Thibault","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801824472","display_name":"Jewish General Hospital","ror":"https://ror.org/056jjra10","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801824472","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405256057","https://openalex.org/I4405279980"]},{"id":"https://openalex.org/I4405267356","display_name":"Lady Davis Institute for Medical Research","ror":"https://ror.org/02c1np254","country_code":"CA","type":"education","lineage":["https://openalex.org/I2801824472","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405256057","https://openalex.org/I4405267356","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"C Thibault","raw_affiliation_strings":["From the Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, 3755 Cote Saint-Catherine Road, Montreal, Quebec, Canada H3T lE2"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, 3755 Cote Saint-Catherine Road, Montreal, Quebec, Canada H3T lE2","institution_ids":["https://openalex.org/I2801824472","https://openalex.org/I4405267356"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113500258","display_name":"J K Chan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801824472","display_name":"Jewish General Hospital","ror":"https://ror.org/056jjra10","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801824472","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405256057","https://openalex.org/I4405279980"]},{"id":"https://openalex.org/I4405267356","display_name":"Lady Davis Institute for Medical Research","ror":"https://ror.org/02c1np254","country_code":"CA","type":"education","lineage":["https://openalex.org/I2801824472","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405256057","https://openalex.org/I4405267356","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"J K Chan","raw_affiliation_strings":["From the Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, 3755 Cote Saint-Catherine Road, Montreal, Quebec, Canada H3T lE2"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, 3755 Cote Saint-Catherine Road, Montreal, Quebec, Canada H3T lE2","institution_ids":["https://openalex.org/I2801824472","https://openalex.org/I4405267356"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024375229","display_name":"James F. Perdue","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801824472","display_name":"Jewish General Hospital","ror":"https://ror.org/056jjra10","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801824472","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405256057","https://openalex.org/I4405279980"]},{"id":"https://openalex.org/I4405267356","display_name":"Lady Davis Institute for Medical Research","ror":"https://ror.org/02c1np254","country_code":"CA","type":"education","lineage":["https://openalex.org/I2801824472","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405256057","https://openalex.org/I4405267356","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"J F Perdue","raw_affiliation_strings":["From the Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, 3755 Cote Saint-Catherine Road, Montreal, Quebec, Canada H3T lE2"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, 3755 Cote Saint-Catherine Road, Montreal, Quebec, Canada H3T lE2","institution_ids":["https://openalex.org/I2801824472","https://openalex.org/I4405267356"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008131820","display_name":"William H. Daughaday","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W H Daughaday","raw_affiliation_strings":["From the Department of Medicine, Metabolism Division, Washington University, St. Louis, Missouri 631 IO"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Medicine, Metabolism Division, Washington University, St. Louis, Missouri 631 IO","institution_ids":["https://openalex.org/I204465549"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.8634,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.82984673,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"259","issue":"5","first_page":"3361","last_page":"3367"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12287","display_name":"Fibroblast Growth Factor Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/insulin-like-growth-factor-ii-receptor","display_name":"insulin-like growth factor II receptor","score":0.9629999995231628},{"id":"https://openalex.org/keywords/igf-ii-binding","display_name":"IGF-II binding","score":0.8320000171661377},{"id":"https://openalex.org/keywords/molecular-weight-determination","display_name":"molecular weight determination","score":0.8339999914169312},{"id":"https://openalex.org/keywords/molecular-radius","display_name":"molecular radius","score":0.9279999732971191},{"id":"https://openalex.org/keywords/native-protein-separation","display_name":"native protein separation","score":0.5270000100135803},{"id":"https://openalex.org/keywords/glycoprotein-structure","display_name":"glycoprotein structure","score":0.7120000123977661},{"id":"https://openalex.org/keywords/disulfide-bonds","display_name":"disulfide bonds","score":0.6970000267028809},{"id":"https://openalex.org/keywords/rat-placenta","display_name":"rat placenta","score":0.4359999895095825}],"concepts":[{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.6902243494987488},{"id":"https://openalex.org/C2778862431","wikidata":"https://www.wikidata.org/wiki/Q416002","display_name":"Polyacrylamide","level":2,"score":0.5881075859069824},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5318716764450073},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.5214087963104248},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.491039514541626},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.4834100008010864},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.44290050864219666},{"id":"https://openalex.org/C35859425","wikidata":"https://www.wikidata.org/wiki/Q384609","display_name":"Gel electrophoresis of proteins","level":4,"score":0.41799238324165344},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.30824413895606995},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.04782530665397644},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.046808987855911255}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010920","descriptor_name":"Placenta","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011247","descriptor_name":"Pregnancy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011956","descriptor_name":"Receptors, Cell Surface","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D012995","descriptor_name":"Solubility","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017451","descriptor_name":"Receptors, Somatomedin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(17)43303-5","doi":"https://doi.org/10.1016/s0021-9258(17)43303-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(17)43303-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:6321506","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6321506","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(17)43303-5","doi":"https://doi.org/10.1016/s0021-9258(17)43303-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(17)43303-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1500990165","https://openalex.org/W1508807714","https://openalex.org/W1513760428","https://openalex.org/W1536119619","https://openalex.org/W1581288183","https://openalex.org/W1590947913","https://openalex.org/W1592676263","https://openalex.org/W1595228756","https://openalex.org/W1602100480","https://openalex.org/W1647519600","https://openalex.org/W2017360965","https://openalex.org/W2026534021","https://openalex.org/W2029508711","https://openalex.org/W2036106259","https://openalex.org/W2038345597","https://openalex.org/W2039938812","https://openalex.org/W2054631692","https://openalex.org/W2073481159","https://openalex.org/W2074041522","https://openalex.org/W2075096741","https://openalex.org/W2087458889","https://openalex.org/W2093748430","https://openalex.org/W2100837269","https://openalex.org/W2149500883","https://openalex.org/W2325349671"],"related_works":["https://openalex.org/W2075096741","https://openalex.org/W1994734038","https://openalex.org/W2060300944","https://openalex.org/W1595228756","https://openalex.org/W2067658546","https://openalex.org/W2084415440","https://openalex.org/W2036961244","https://openalex.org/W1969127350","https://openalex.org/W2011133280","https://openalex.org/W1595594310"],"abstract_inverted_index":{"High":[0],"resolution":[1],"quantitative":[2,236],"polyacrylamide":[3,93],"gel":[4,42,128,176,268],"electrophoresis":[5,269],"was":[6,70,111,194,220],"employed":[7],"under":[8],"nondenaturing":[9],"conditions":[10],"to":[11,100,131,174,222],"calculate":[12],"a":[13,161,190,197,202,224,230,277,288],"molecular":[14,148,210],"weight,":[15,149],"Mr,":[16,150],"for":[17,178,182],"rat":[18],"placental":[19],"membrane":[20],"receptors":[21,33],"that":[22,34,238,274],"bind":[23],"insulin-like":[24],"growth":[25],"factor":[26],"II":[27],"(IGF-II).":[28],"An":[29],"n-octylglucoside-soluble":[30],"extract":[31],"of":[32,79,82,107,126,141,160,163,165,172,184,204,213,226,233,259,261,290],"had":[35],"been":[36],"enriched":[37],"20-fold":[38],"during":[39],"Sephacryl":[40],"S-300":[41],"chromatography":[43],"and":[44,60,85,116,136,181,229,246,270,286],"designated":[45],"peak":[46,106],"1":[47],"(Perdue,":[48],"J.":[49,52,65],"F.,":[50],"Chan,":[51],"K.,":[53],"Thibault,":[54],"C.,":[55],"Radaj,":[56],"P.,":[57],"Mills,":[58],"B.,":[59],"Daughaday,":[61],"W.":[62],"H.":[63],"(1983)":[64],"Biol.":[66],"Chem.":[67],"258,":[68],"7800-7811)":[69],"incubated":[71],"with":[72,133,256,287],"125I-IGF-II":[73,132],"in":[74,87,138,253],"the":[75,118,124,127,139,146,152,158,166,179,209,214,217,257,262,272],"presence":[76,140],"or":[77],"absence":[78],"an":[80],"excess":[81],"unlabeled":[83],"IGF-II":[84,108,155,218],"electrophoresed":[86,137],"glass":[88],"tubes":[89],"containing":[90],"highly":[91],"cross-linked":[92,130],"gels":[94],"at":[95,170],"concentrations":[96,177],"ranging":[97],"from":[98,123,201],"5":[99],"12%":[101],"(w/v).":[102],"A":[103],"specifically":[104],"labeled":[105],"binding":[109],"activity":[110],"identified":[112],"by":[113,264,283],"freezing,":[114],"slicing,":[115],"counting":[117],"gels.":[119],"The":[120],"proteins":[121,187,241],"eluted":[122],"region":[125],"when":[129],"disuccinimidyl":[134],"suberate":[135],"sodium":[142,265],"dodecyl":[143,266],"sulfate":[144],"have":[145,223,248],"same":[147],"as":[151],"previously":[153],"described":[154],"receptor.":[156],"From":[157],"slope":[159],"plot":[162,203],"log":[164],"relative":[167],"mobility,":[168],"RF,":[169],"each":[171,183],"6":[173],"7":[175],"125I-IGF-II-receptor":[180],"seven":[185],"standard":[186,215],"(Ferguson-Hedrick":[188],"plot),":[189],"retardation":[191],"coefficient,":[192],"KR,":[193],"determined.":[195],"Using":[196],"reference":[198],"curve":[199],"constructed":[200],"square":[205],"root":[206],"KR":[207],"versus":[208],"radius,":[211],"R,":[212],"proteins,":[216],"receptor":[219],"estimated":[221],"R":[225],"4.13":[227],"nm":[228],"calculated":[231],"Mr":[232],"250,000.":[234],"Thus,":[235],"procedures":[237],"separate":[239],"native":[240],"based":[242],"on":[243],"their":[244],"size":[245],"charge":[247],"provided":[249],"information":[250],"which":[251],"is":[252,276],"good":[254],"agreement":[255],"results":[258],"studies":[260],"IGF-II-receptor":[263],"sulfate-polyacrylamide":[267],"supports":[271],"premise":[273],"it":[275],"monomeric":[278],"single":[279],"chained":[280],"glycoprotein,":[281],"constrained":[282],"intradisulfide":[284],"bonds":[285],"mass":[289],"250":[291],"kDa.":[292]},"counts_by_year":[],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}