{"id":"https://openalex.org/W1586783995","doi":null,"title":"AFM investigations of critical interactions in the Bacillus primosome and Cryogenic AFM a new tool for structural biology","display_name":"AFM investigations of critical interactions in the Bacillus primosome and Cryogenic AFM a new tool for structural biology","publication_year":2006,"publication_date":"2006-01-01","ids":{"openalex":"https://openalex.org/W1586783995","mag":"1586783995"},"language":"en","primary_location":{"id":"pmh:oai:eprints.nottingham.ac.uk:10188","doi":null,"is_oa":true,"landing_page_url":"https://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","pdf_url":"http://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","source":{"id":"https://openalex.org/S4306402014","display_name":"Nottingham ePrints (University of Nottingham)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I142263535","host_organization_name":"University of Nottingham","host_organization_lineage":["https://openalex.org/I142263535"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis (University of Nottingham only)"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Ian Turner","orcid":"https://orcid.org/0000-0002-5070-6621","observed_orcids":["https://orcid.org/0000-0002-5070-6621"]},"institutions":[{"id":"https://openalex.org/I142263535","display_name":"University of Nottingham","ror":"https://ror.org/01ee9ar58","country_code":"GB","type":"education","lineage":["https://openalex.org/I142263535"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Ian Turner","raw_affiliation_strings":["University of Nottingham"],"raw_orcid":"https://orcid.org/0000-0002-5070-6621","affiliations":[{"raw_affiliation_string":"University of Nottingham","institution_ids":["https://openalex.org/I142263535"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I142263535"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.9672999978065491,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.9672999978065491,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.008999999612569809,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.00800000037997961,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/atomic-force-microscopy","display_name":"atomic force microscopy","score":0.8519999980926514},{"id":"https://openalex.org/keywords/dna-supercoiling","display_name":"DNA supercoiling","score":0.8859999775886536},{"id":"https://openalex.org/keywords/h-ns","display_name":"H-NS","score":0.7350000143051147},{"id":"https://openalex.org/keywords/protein-dna-interactions","display_name":"protein-DNA interactions","score":0.8849999904632568},{"id":"https://openalex.org/keywords/protein-oligomerization","display_name":"protein oligomerization","score":0.44699999690055847}],"concepts":[{"id":"https://openalex.org/C189754071","wikidata":"https://www.wikidata.org/wiki/Q7291972","display_name":"Random hexamer","level":2,"score":0.8335614800453186},{"id":"https://openalex.org/C102951782","wikidata":"https://www.wikidata.org/wiki/Q49295","display_name":"Atomic force microscopy","level":2,"score":0.6627516746520996},{"id":"https://openalex.org/C2776403692","wikidata":"https://www.wikidata.org/wiki/Q898416","display_name":"Trimer","level":3,"score":0.6153194904327393},{"id":"https://openalex.org/C194830204","wikidata":"https://www.wikidata.org/wiki/Q106940703","display_name":"Primase","level":5,"score":0.5805652141571045},{"id":"https://openalex.org/C161223559","wikidata":"https://www.wikidata.org/wiki/Q138864","display_name":"Helicase","level":4,"score":0.5228450894355774},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5031551718711853},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.483576238155365},{"id":"https://openalex.org/C191120209","wikidata":"https://www.wikidata.org/wiki/Q908902","display_name":"Structural biology","level":2,"score":0.45901575684547424},{"id":"https://openalex.org/C2779546866","wikidata":"https://www.wikidata.org/wiki/Q531306","display_name":"Dimer","level":2,"score":0.45756298303604126},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.453086256980896},{"id":"https://openalex.org/C2777331810","wikidata":"https://www.wikidata.org/wiki/Q3374897","display_name":"Pentamer","level":2,"score":0.44965434074401855},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4456876218318939},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.43548792600631714},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.38529855012893677},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3562520146369934},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24379116296768188},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17611852288246155},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"pmh:oai:eprints.nottingham.ac.uk:10188","doi":null,"is_oa":true,"landing_page_url":"https://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","pdf_url":"http://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","source":{"id":"https://openalex.org/S4306402014","display_name":"Nottingham ePrints (University of Nottingham)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I142263535","host_organization_name":"University of Nottingham","host_organization_lineage":["https://openalex.org/I142263535"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis (University of Nottingham only)"},{"id":"pmh:oai:repository.nottingham.ac.uk:123456789/50228","doi":null,"is_oa":true,"landing_page_url":"https://repository.nottingham.ac.uk/handle/123456789/50228","pdf_url":"https://repository.nottingham.ac.uk/bitstreams/d7b71feb-4e99-4513-96e1-c7df67e73c2a/download","source":{"id":"https://openalex.org/S4306402014","display_name":"Nottingham ePrints (University of Nottingham)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I142263535","host_organization_name":"University of Nottingham","host_organization_lineage":["https://openalex.org/I142263535"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"ethesis"},{"id":"pmh:hdl:10068/967571","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10068/967571","pdf_url":null,"source":{"id":"https://openalex.org/S4377196900","display_name":"OpenGrey (Institut de l'Information Scientifique et Technique)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1295345811","host_organization_name":"Institut de l'Information Scientifique et Technique","host_organization_lineage":["https://openalex.org/I1295345811"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"U - Thesis"},{"id":"pmh:oai:uilis.unsyiah.ac.id:193","doi":null,"is_oa":false,"landing_page_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/193","pdf_url":null,"source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis (University of Nottingham only)"},{"id":"mag:1586783995","doi":null,"is_oa":false,"landing_page_url":"http://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:eprints.nottingham.ac.uk:10188","doi":null,"is_oa":true,"landing_page_url":"https://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","pdf_url":"http://eprints.nottingham.ac.uk/10188/1/Thesis_Complete_iant.pdf","source":{"id":"https://openalex.org/S4306402014","display_name":"Nottingham ePrints (University of Nottingham)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I142263535","host_organization_name":"University of Nottingham","host_organization_lineage":["https://openalex.org/I142263535"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis (University of Nottingham only)"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1586783995.pdf","grobid_xml":"https://content.openalex.org/works/W1586783995.grobid-xml"},"referenced_works_count":186,"referenced_works":["https://openalex.org/W254593455","https://openalex.org/W641828379","https://openalex.org/W1009707322","https://openalex.org/W1496368635","https://openalex.org/W1503194549","https://openalex.org/W1509855954","https://openalex.org/W1532010455","https://openalex.org/W1557642928","https://openalex.org/W1697053366","https://openalex.org/W1800303027","https://openalex.org/W1821871873","https://openalex.org/W1886256750","https://openalex.org/W1946364522","https://openalex.org/W1964726331","https://openalex.org/W1969435183","https://openalex.org/W1970881542","https://openalex.org/W1971031787","https://openalex.org/W1972002950","https://openalex.org/W1974116961","https://openalex.org/W1974124121","https://openalex.org/W1976264185","https://openalex.org/W1976863089","https://openalex.org/W1977046374","https://openalex.org/W1977794796","https://openalex.org/W1977892950","https://openalex.org/W1981248564","https://openalex.org/W1981482769","https://openalex.org/W1982873582","https://openalex.org/W1984894450","https://openalex.org/W1985972389","https://openalex.org/W1986784767","https://openalex.org/W1987170498","https://openalex.org/W1987697568","https://openalex.org/W1989409728","https://openalex.org/W1989692639","https://openalex.org/W1992394095","https://openalex.org/W1993651081","https://openalex.org/W1993749221","https://openalex.org/W1997161926","https://openalex.org/W1997827330","https://openalex.org/W1999058709","https://openalex.org/W2001496871","https://openalex.org/W2002255642","https://openalex.org/W2003030256","https://openalex.org/W2003336032","https://openalex.org/W2003764421","https://openalex.org/W2004392681","https://openalex.org/W2004586929","https://openalex.org/W2005053200","https://openalex.org/W2006711543","https://openalex.org/W2007140097","https://openalex.org/W2009740033","https://openalex.org/W2009801723","https://openalex.org/W2010912319","https://openalex.org/W2011733534","https://openalex.org/W2012230395","https://openalex.org/W2013854174","https://openalex.org/W2017397675","https://openalex.org/W2018325248","https://openalex.org/W2018775916","https://openalex.org/W2019904287","https://openalex.org/W2022834881","https://openalex.org/W2023887881","https://openalex.org/W2025757288","https://openalex.org/W2026378678","https://openalex.org/W2029428337","https://openalex.org/W2029877483","https://openalex.org/W2030903100","https://openalex.org/W2031342986","https://openalex.org/W2031700153","https://openalex.org/W2032641879","https://openalex.org/W2033128895","https://openalex.org/W2034175604","https://openalex.org/W2034958496","https://openalex.org/W2035469525","https://openalex.org/W2036108825","https://openalex.org/W2037014396","https://openalex.org/W2038054291","https://openalex.org/W2038600320","https://openalex.org/W2040037961","https://openalex.org/W2040291578","https://openalex.org/W2040695115","https://openalex.org/W2040703270","https://openalex.org/W2040810713","https://openalex.org/W2041262004","https://openalex.org/W2041673104","https://openalex.org/W2043736119","https://openalex.org/W2044966229","https://openalex.org/W2045696781","https://openalex.org/W2046657106","https://openalex.org/W2047900294","https://openalex.org/W2049537473","https://openalex.org/W2050721983","https://openalex.org/W2052221476","https://openalex.org/W2053051285","https://openalex.org/W2054100435","https://openalex.org/W2057041903","https://openalex.org/W2057455539","https://openalex.org/W2058322547","https://openalex.org/W2059541577","https://openalex.org/W2060006567","https://openalex.org/W2061135731","https://openalex.org/W2061543408","https://openalex.org/W2061551682","https://openalex.org/W2062201957","https://openalex.org/W2065112109","https://openalex.org/W2066340610","https://openalex.org/W2066531378","https://openalex.org/W2066638568","https://openalex.org/W2068549917","https://openalex.org/W2068696826","https://openalex.org/W2069178475","https://openalex.org/W2070088962","https://openalex.org/W2070196580","https://openalex.org/W2072556211","https://openalex.org/W2072932270","https://openalex.org/W2073624860","https://openalex.org/W2073657545","https://openalex.org/W2075502902","https://openalex.org/W2076561679","https://openalex.org/W2077773545","https://openalex.org/W2077943655","https://openalex.org/W2079301441","https://openalex.org/W2079361294","https://openalex.org/W2079619493","https://openalex.org/W2080014512","https://openalex.org/W2081212207","https://openalex.org/W2082240398","https://openalex.org/W2084522254","https://openalex.org/W2084563793","https://openalex.org/W2086187114","https://openalex.org/W2087589993","https://openalex.org/W2087894478","https://openalex.org/W2091276963","https://openalex.org/W2092187137","https://openalex.org/W2093225985","https://openalex.org/W2093984841","https://openalex.org/W2100127593","https://openalex.org/W2101957389","https://openalex.org/W2105110539","https://openalex.org/W2107302396","https://openalex.org/W2107511168","https://openalex.org/W2108846862","https://openalex.org/W2109771891","https://openalex.org/W2110245737","https://openalex.org/W2111745935","https://openalex.org/W2117797126","https://openalex.org/W2122571070","https://openalex.org/W2128610137","https://openalex.org/W2132695846","https://openalex.org/W2135625588","https://openalex.org/W2135635018","https://openalex.org/W2136440044","https://openalex.org/W2138997827","https://openalex.org/W2139117182","https://openalex.org/W2139316119","https://openalex.org/W2141291479","https://openalex.org/W2142567415","https://openalex.org/W2143987581","https://openalex.org/W2145320519","https://openalex.org/W2146841255","https://openalex.org/W2149271478","https://openalex.org/W2149959193","https://openalex.org/W2158661016","https://openalex.org/W2161094969","https://openalex.org/W2161281573","https://openalex.org/W2161761398","https://openalex.org/W2163884154","https://openalex.org/W2165141615","https://openalex.org/W2165554797","https://openalex.org/W2165915678","https://openalex.org/W2168543199","https://openalex.org/W2171046836","https://openalex.org/W2179107786","https://openalex.org/W2183359834","https://openalex.org/W2187478964","https://openalex.org/W2306853147","https://openalex.org/W2331470031","https://openalex.org/W2408621604","https://openalex.org/W2412077657","https://openalex.org/W2414936243","https://openalex.org/W2421838664","https://openalex.org/W2568203515","https://openalex.org/W3140246860","https://openalex.org/W3147740451","https://openalex.org/W3150248096"],"related_works":["https://openalex.org/W7006766924","https://openalex.org/W2012230395","https://openalex.org/W2106352716","https://openalex.org/W3097715506","https://openalex.org/W2049537473","https://openalex.org/W7140203317","https://openalex.org/W2324839412","https://openalex.org/W2120910075","https://openalex.org/W2783519823","https://openalex.org/W2024356919"],"abstract_inverted_index":{"In":[0,176],"this":[1,177,185,225,338],"thesis":[2,178,339,376],"for":[3,10,152,297,351,379,414],"the":[4,11,26,31,44,54,60,63,66,69,90,114,123,137,147,164,202,208,245,250,253,268,298,315,319,352,380,398,415,424],"first":[5,124,381],"AFM":[6,45,119,127,151,179,263,295,326,336,384,399,408],"has":[7,109,192,314],"been":[8,110,132],"employed":[9],"high":[12,353],"resolution":[13,49,154,354,391],"imaging":[14,155,269,299,355,416],"of":[15,53,62,68,79,92,104,140,150,156,161,207,239,247,252,270,291,300,335,356,392,397,417,426],"a":[16,47,74,77,101,141,193,292,312,344,364,410],"protein":[17,142,158,359,393,418],"assembly.":[18],"The":[19,395],"DnaB-DnaG":[20],"Helicase-Primase":[21],"interaction":[22],"in":[23,163,196,337,346],"Bacillus":[24,165],"is":[25,57,122,167,409],"key":[27],"reaction":[28,226],"that":[29,59,112,126,190,262,283,310,366,383,407],"causes":[30,65],"switch":[32,72],"from":[33,73],"primase":[34,64,83],"mode":[35,103],"to":[36,46,71,76,86,135,182,210,215,219,229,236,267,279,317,371,388],"polymerisation":[37],"mode.":[38],"This":[39,121,375],"assembly":[40,143],"was":[41,174,180,213,227,305],"imaged":[42],"using":[43],"sub-molecular":[48,153,390],"revealing":[50],"structural":[51,115],"detail":[52],"interaction.":[55],"It":[56],"shown":[58,214,228],"binding":[61],"structure":[67,139],"helicase":[70],"hexamer":[75],"trimer":[78],"dimers":[80],"with":[81,94,275,403],"one":[82,95],"molecule":[84],"bound":[85,99],"each":[87],"dimer;":[88],"also":[89,362,377],"existance":[91],"sub-populations":[93],"and":[96,128,144,233,249,257,272,277,289,330,358],"two":[97,320],"primases":[98],"suggests":[100],"sequential":[102],"binding.":[105],"Recently":[106],"crystallography":[107,129,404],"data":[108,130,400,405],"published":[111],"confirms":[113],"observations":[116],"generated":[117],"by":[118],"here.":[120],"time":[125,382],"have":[131],"used":[133,278],"concurrently":[134],"solve":[136,184,280,372],"molecular":[138,347],"it":[145,188,420],"shows":[146,340,378,406],"potential":[148,316],"application":[149,334],"other":[157,205,284],"assemblies.\\n\\nThe":[159],"role":[160,195,246],"DnaD":[162,191,212,248],"primosome":[166,198],"well":[168],"established,":[169],"however,":[170],"its":[171,341],"exact":[172],"function":[173],"unknown.":[175],"applied":[181,266,370,387],"help":[183],"biomolecular":[186,281,373],"problem,":[187],"revealed":[189],"pivotal":[194],"early":[197],"assembly,":[199],"opening":[200],"up":[201],"DNA":[203,218,276],"allowing":[204],"components":[206],"cascade":[209],"bind.":[211],"cause":[216],"supercoiled":[217],"adopt":[220],"an":[221],"open":[222],"circular":[223],"formation;":[224],"be":[230,265,368,386],"both":[231],"reversible":[232],"universally":[234],"applicable":[235],"all":[237],"sequences":[238],"DNA.":[240],"Comparisons":[241],"are":[242],"made":[243],"between":[244],"roles":[251],"histone-like":[254],"proteins":[255,271,357],"H-NS":[256],"HU.":[258],"These":[259],"experiments":[260],"show":[261,309],"can":[264,367,385],"their":[273],"interactions":[274],"problems":[282],"techniques":[285],"cannot":[286],"solve.\\n\\nThe":[287],"design":[288],"implementation":[290],"novel":[293],"cryogenic":[294],"system":[296,313],"biomolecules":[301],"at":[302],"subzero":[303],"temperatures":[304],"executed.":[306],"Preliminary":[307],"results":[308],"such":[311],"reduce":[318],"main":[321],"intrinsic":[322],"effects":[323],"limiting":[324],"current":[325],"imaging;":[327],"sample":[328],"softness":[329],"thermal":[331],"motion.":[332],"\\nThe":[333],"strength":[342,396],"as":[343,363],"tool":[345,413],"biology":[348],"not":[349],"only":[350],"assemblies":[360],"but":[361],"technique":[365,425],"uniquely":[369],"problems.":[374],"generate":[389],"assemblies.":[394],"when":[401],"combined":[402],"very":[411],"powerful":[412],"assemblies;":[419],"could":[421],"even":[422],"become":[423],"choice":[427]},"counts_by_year":[{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2016-06-24T00:00:00"}