{"id":"https://openalex.org/W1585541396","doi":"https://doi.org/10.1016/s0021-9258(19)69517-7","title":"Differential labeling and identification of the cysteine-containing tryptic peptides of catalytic subunit from porcine heart cAMP-dependent protein kinase.","display_name":"Differential labeling and identification of the cysteine-containing tryptic peptides of catalytic subunit from porcine heart cAMP-dependent protein kinase.","publication_year":1981,"publication_date":"1981-04-01","ids":{"openalex":"https://openalex.org/W1585541396","doi":"https://doi.org/10.1016/s0021-9258(19)69517-7","mag":"1585541396","pmid":"https://pubmed.ncbi.nlm.nih.gov/6260776"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)69517-7","doi":"https://doi.org/10.1016/s0021-9258(19)69517-7","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)69517-7","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)69517-7","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"N.C. Nelson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N.C. Nelson","raw_affiliation_strings":["From the Department of Chemistry, University of California, Sun Diego, La Jolla, California 92093"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Chemistry, University of California, Sun Diego, La Jolla, California 92093","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045962448","display_name":"Susan Serota Taylor","orcid":"https://orcid.org/0000-0002-7702-6108","observed_orcids":["https://orcid.org/0000-0002-7702-6108"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S.S. Taylor","raw_affiliation_strings":["From the Department of Chemistry, University of California, Sun Diego, La Jolla, California 92093"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Chemistry, University of California, Sun Diego, La Jolla, California 92093","institution_ids":["https://openalex.org/I36258959"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I36258959"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.8228,"has_fulltext":false,"cited_by_count":81,"citation_normalized_percentile":{"value":0.90781199,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"256","issue":"8","first_page":"3743","last_page":"3750"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.8363999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.8363999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.053599998354911804,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.029200000688433647,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-kinase-a","display_name":"protein kinase A","score":0.9599999785423279},{"id":"https://openalex.org/keywords/catalytic-subunit","display_name":"catalytic subunit","score":0.996999979019165},{"id":"https://openalex.org/keywords/cysteine-containing-peptides","display_name":"cysteine-containing peptides","score":0.8889999985694885},{"id":"https://openalex.org/keywords/tryptic-peptides","display_name":"tryptic peptides","score":0.9319999814033508},{"id":"https://openalex.org/keywords/peptide-identification","display_name":"peptide identification","score":0.527999997138977},{"id":"https://openalex.org/keywords/porcine-heart","display_name":"porcine heart","score":0.8330000042915344},{"id":"https://openalex.org/keywords/sulfhydryl-groups","display_name":"sulfhydryl groups","score":0.734000027179718}],"concepts":[{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.7119308710098267},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.6272660493850708},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.5999528169631958},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5520772933959961},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5255864262580872},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.42356303334236145},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.40329647064208984},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34537994861602783},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28763413429260254},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2760958671569824},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1188879907131195},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000242","descriptor_name":"Cyclic AMP","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003545","descriptor_name":"Cysteine","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D007461","descriptor_name":"Iodoacetates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009206","descriptor_name":"Myocardium","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)69517-7","doi":"https://doi.org/10.1016/s0021-9258(19)69517-7","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)69517-7","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:6260776","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6260776","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)69517-7","doi":"https://doi.org/10.1016/s0021-9258(19)69517-7","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)69517-7","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W166487155","https://openalex.org/W179113440","https://openalex.org/W184399086","https://openalex.org/W1483067155","https://openalex.org/W1490238718","https://openalex.org/W1521048080","https://openalex.org/W1522702062","https://openalex.org/W1525197059","https://openalex.org/W1567804644","https://openalex.org/W1568303936","https://openalex.org/W1571793851","https://openalex.org/W1580433758","https://openalex.org/W1598316265","https://openalex.org/W1602931525","https://openalex.org/W1649480412","https://openalex.org/W1954353020","https://openalex.org/W1976863757","https://openalex.org/W1979111516","https://openalex.org/W1987366145","https://openalex.org/W1996302033","https://openalex.org/W2030349770","https://openalex.org/W2037362840","https://openalex.org/W2040456790","https://openalex.org/W2048944429","https://openalex.org/W2062107117","https://openalex.org/W2080240343","https://openalex.org/W2085119576","https://openalex.org/W2150453547","https://openalex.org/W2240965888","https://openalex.org/W2339383930","https://openalex.org/W2395478297","https://openalex.org/W2608470872","https://openalex.org/W4246526382"],"related_works":["https://openalex.org/W2056867084","https://openalex.org/W2079791947","https://openalex.org/W2463971649","https://openalex.org/W2058632201","https://openalex.org/W1515082404","https://openalex.org/W2901320324","https://openalex.org/W137979976","https://openalex.org/W1532275854","https://openalex.org/W2143884639","https://openalex.org/W2057631371"],"abstract_inverted_index":{"The":[0],"cysteine-containing":[1],"tryptic":[2],"peptides":[3,85],"from":[4],"the":[5,17,20,26],"catalytic":[6,27],"subunit":[7,28],"of":[8,19,45,60,73,82],"CAMP-dependent":[9],"protein":[10],"kinase":[11],"were":[12,51,54],"isolated":[13],"and":[14,37,49,65,77],"characterized":[15],"with":[16,31,35],"aid":[18],"sulfhydryl":[21],"specific":[22],"reagent":[23],"iodoacetic":[24],"acid.When":[25],"was":[29],"reacted":[30],"['4C)iodoacetic":[32],"acid,":[33],"cleaved":[34],"trypsin,":[36],"chromatographed":[38],"on":[39],"Sephadex":[40],"G-50,":[41],"three":[42],"distinct":[43],"components":[44,53,75],"radioactivity":[46],"(C-1,":[47],"C-2,":[48],"C-3)":[50],"identified.These":[52],"further":[55],"purified":[56],"using":[57],"a":[58],"combination":[59],"high":[61],"performance":[62],"liquid":[63],"chromatography":[64],"paper":[66],"chromatography.Subsequent":[67],"analysis":[68],"revealed":[69],"that":[70],"only":[71],"two":[72,84],"these":[74,83],"((3-2":[76],"(2-3)":[78],"contained":[79],"carboxymethylcysteine.The":[80],"sequences":[81],"are":[86],"as":[87],"follows:":[88],"(C-2)":[89],"-Cys-Gly-Lys-Glu-Phe-Ser-Glu-Phe;":[90]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}