{"id":"https://openalex.org/W157803802","doi":null,"title":"Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies","display_name":"Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies","publication_year":2006,"publication_date":"2006-01-01","ids":{"openalex":"https://openalex.org/W157803802","mag":"157803802"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:uu-7320","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7320","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7320","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015423300","display_name":"Amal Elfaitouri","orcid":"https://orcid.org/0000-0002-0704-3252","observed_orcids":["https://orcid.org/0000-0002-0704-3252"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Amal Elfaitouri","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-0704-3252","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5015423300"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9185000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9185000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11112","display_name":"Viral Infections and Immunology Research","score":0.037700001150369644,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.018300000578165054,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/quantitative-real-time-pcr","display_name":"quantitative real-time PCR","score":0.9190000295639038},{"id":"https://openalex.org/keywords/viral-diagnostics","display_name":"viral diagnostics","score":0.5329999923706055},{"id":"https://openalex.org/keywords/enterovirus-detection","display_name":"enterovirus detection","score":0.7070000171661377},{"id":"https://openalex.org/keywords/aseptic-meningitis","display_name":"aseptic meningitis","score":0.9660000205039978},{"id":"https://openalex.org/keywords/igm","display_name":"IgM","score":0.6809999942779541},{"id":"https://openalex.org/keywords/jc-virus","display_name":"JC virus","score":0.9139999747276306},{"id":"https://openalex.org/keywords/bk-polyomavirus","display_name":"BK virus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/simian-virus-40","display_name":"simian virus 40","score":0.9890000224113464},{"id":"https://openalex.org/keywords/type-1-diabetes","display_name":"type 1 diabetes","score":0.7919999957084656},{"id":"https://openalex.org/keywords/vp2-gene","display_name":"VP2 gene","score":0.7519999742507935}],"concepts":[{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6852519512176514},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5364160537719727},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.47407856583595276},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.4194129407405853},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4085710048675537},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3639797568321228},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.32919561862945557},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1510951817035675},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13625827431678772},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13113680481910706}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:uu-7320","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7320","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:157803802","doi":null,"is_oa":false,"landing_page_url":"http://uu.diva-portal.org/smash/get/diva2:169270/FULLTEXT01.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:uu-7320","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7320","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9391000270843506}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":131,"referenced_works":["https://openalex.org/W9819090","https://openalex.org/W40403310","https://openalex.org/W51002086","https://openalex.org/W178710137","https://openalex.org/W201050536","https://openalex.org/W203297188","https://openalex.org/W1489487899","https://openalex.org/W1517480469","https://openalex.org/W1555039120","https://openalex.org/W1569947224","https://openalex.org/W1571934973","https://openalex.org/W1735834838","https://openalex.org/W1943449996","https://openalex.org/W1965167039","https://openalex.org/W1968160106","https://openalex.org/W1969355897","https://openalex.org/W1970558970","https://openalex.org/W1970947525","https://openalex.org/W1971377917","https://openalex.org/W1974085494","https://openalex.org/W1974625287","https://openalex.org/W1974844022","https://openalex.org/W1975962007","https://openalex.org/W1980049784","https://openalex.org/W1987176207","https://openalex.org/W1989209244","https://openalex.org/W1989765696","https://openalex.org/W1993700655","https://openalex.org/W1994674027","https://openalex.org/W1996044268","https://openalex.org/W1997242660","https://openalex.org/W2000252731","https://openalex.org/W2001085828","https://openalex.org/W2001526641","https://openalex.org/W2001781773","https://openalex.org/W2012491681","https://openalex.org/W2013092976","https://openalex.org/W2013241878","https://openalex.org/W2013643741","https://openalex.org/W2013726567","https://openalex.org/W2019548301","https://openalex.org/W2019703209","https://openalex.org/W2020230677","https://openalex.org/W2020320734","https://openalex.org/W2022227920","https://openalex.org/W2023367287","https://openalex.org/W2028981905","https://openalex.org/W2032118018","https://openalex.org/W2033028088","https://openalex.org/W2034449046","https://openalex.org/W2038432589","https://openalex.org/W2038635248","https://openalex.org/W2039148312","https://openalex.org/W2043708736","https://openalex.org/W2049685599","https://openalex.org/W2050717506","https://openalex.org/W2052765688","https://openalex.org/W2053592833","https://openalex.org/W2057023719","https://openalex.org/W2058456674","https://openalex.org/W2061192012","https://openalex.org/W2061604613","https://openalex.org/W2062187112","https://openalex.org/W2062991200","https://openalex.org/W2064922726","https://openalex.org/W2066380514","https://openalex.org/W2070019379","https://openalex.org/W2070721758","https://openalex.org/W2072652145","https://openalex.org/W2076543147","https://openalex.org/W2077133634","https://openalex.org/W2081492099","https://openalex.org/W2082176280","https://openalex.org/W2083313788","https://openalex.org/W2088350742","https://openalex.org/W2089487509","https://openalex.org/W2091330414","https://openalex.org/W2091610290","https://openalex.org/W2094835280","https://openalex.org/W2096090669","https://openalex.org/W2100187217","https://openalex.org/W2102279421","https://openalex.org/W2103219789","https://openalex.org/W2103915337","https://openalex.org/W2104451450","https://openalex.org/W2105979235","https://openalex.org/W2106700932","https://openalex.org/W2108545791","https://openalex.org/W2108794386","https://openalex.org/W2109127763","https://openalex.org/W2109760469","https://openalex.org/W2110784088","https://openalex.org/W2112725402","https://openalex.org/W2118537438","https://openalex.org/W2123284183","https://openalex.org/W2125092867","https://openalex.org/W2127065841","https://openalex.org/W2131758886","https://openalex.org/W2132537748","https://openalex.org/W2134343377","https://openalex.org/W2137368421","https://openalex.org/W2140249326","https://openalex.org/W2144677167","https://openalex.org/W2146668907","https://openalex.org/W2146913304","https://openalex.org/W2147275901","https://openalex.org/W2150210328","https://openalex.org/W2152838075","https://openalex.org/W2154306061","https://openalex.org/W2156716354","https://openalex.org/W2156735755","https://openalex.org/W2157815953","https://openalex.org/W2158307330","https://openalex.org/W2159856736","https://openalex.org/W2161783062","https://openalex.org/W2161942532","https://openalex.org/W2164578725","https://openalex.org/W2165739520","https://openalex.org/W2224780480","https://openalex.org/W2337870848","https://openalex.org/W2405006178","https://openalex.org/W2407245124","https://openalex.org/W2409967970","https://openalex.org/W2410881499","https://openalex.org/W2411262013","https://openalex.org/W2415524476","https://openalex.org/W2418308331","https://openalex.org/W2425577600","https://openalex.org/W2430068526","https://openalex.org/W2470655868","https://openalex.org/W7098124190"],"related_works":["https://openalex.org/W2014035551","https://openalex.org/W2156716354","https://openalex.org/W2103915337","https://openalex.org/W2065117942","https://openalex.org/W1992076170","https://openalex.org/W2108295257","https://openalex.org/W2407388577","https://openalex.org/W2086268240","https://openalex.org/W2898701738","https://openalex.org/W1544171766"],"abstract_inverted_index":{"Quantitative":[0],"real-time":[1,62,146,278],"PCR":[2,279],"(QPCR)":[3],"technology":[4],"has":[5,15],"been":[6],"very":[7],"useful":[8],"for":[9,41,104,128,159,186,236,281],"diagnosis":[10,160,282],"of":[11,20,30,71,73,84,106,112,130,161,188,199,203,238,244,283],"viral":[12,108,113,245,287],"diseases.":[13],"QPCR":[14,54,184],"recently":[16],"reached":[17],"a":[18,27,38,124,144,176,233],"level":[19],"sensitivity,":[21],"simplicity,":[22],"and":[23,51,89,191,206,270,285],"reproducibility":[24],"which":[25],"allows":[26,241],"large":[28],"number":[29],"samples":[31],"to":[32,77,155,215,256],"be":[33],"screened":[34],"rapidly,":[35],"make":[36],"it":[37],"suitable":[39],"tool":[40],"the":[42,82,121,141,173,200,229,242],"clinical":[43],"virology":[44],"diagnostics.":[45],"In":[46,120,140,172,226],"this":[47],"thesis,":[48],"broadly":[49,177,276],"targeted":[50,277],"degenerated":[52,182],"quantitative":[53,152,183],"assays":[55],"were":[56,289],"used.":[57],"A":[58,101,196,210],"somewhat":[59],"novel":[60,275],"single-tube":[61,126],"reverse":[63,78],"transcription-polymerase":[64],"chain":[65],"reaction":[66],"(QRT-PCR),":[67],"with":[68,135,166,217],"takes":[69],"advantage":[70],"ability":[72],"rTth":[74],"DNA":[75,193],"polymerase":[76],"transcribe":[79],"RNA":[80,132],"in":[81,133,164,262],"presence":[83],"Mn2+":[85],"at":[86],"elevated":[87],"temperatures":[88],"includes":[90],"protection":[91],"against":[92,259],"amplimer":[93],"contamination":[94,216],"by":[95,110],"using":[96],"thermolabile":[97],"UNG,":[98],"was":[99,117,138,169,194,208,223],"developed.":[100,119,171,195,290],"new":[102],"technique":[103,185],"diagnostic":[105],"recent":[107],"infection":[109,163],"detection":[111,129,187,237],"immunoglobulin":[114],"M":[115],"(IgM)":[116],"also":[118,170],"first":[122],"paper,":[123,143,175],"sensitive":[125],"QRT-PCR":[127],"enteroviral":[131],"patients":[134,165,254],"aseptic":[136,167],"meningitis":[137],"presented.":[139],"second":[142],"single-serum-dilution":[145],"PCR-based":[147],"PIA":[148,153],"(PCR-enhanced":[149],"immunoassay),":[150],"called":[151],"(QPIA),":[154],"detect":[156],"enterovirus":[157],"IgM":[158,258],"EV":[162,239],"meningitis,":[168],"third":[174],"targeted,":[178],"simple,":[179],"single":[180],"tube":[181],"JCV,":[189,204],"BKV":[190,205],"SV40":[192,207,220],"conserved":[197],"region":[198],"VP2":[201],"gene":[202],"targeted.":[209],"false":[211],"positive":[212],"result":[213],"due":[214],"commonly":[218],"used":[219],"T-antigen":[221],"plasmids":[222],"therefore":[224],"avoided.":[225],"manuscript":[227],"four,":[228],"QPIA":[230],"assay":[231],"provide":[232],"rational":[234],"strategy":[235],"IgM,":[240],"use":[243],"antigens":[246],"isolate":[247],"from":[248,264],"newly":[249,265],"diagnosed":[250,266],"Type":[251],"1":[252],"diabetes":[253],"(T1D-EV-QPIA)":[255],"measured":[257],"diabetogenic":[260],"viruses":[261],"serum":[263],"T1D":[267],"children,":[268],"siblings,":[269],"healthy":[271],"children.":[272],"To":[273],"conclude,":[274],"methods":[280],"entero-":[284],"polyoma":[286],"infections":[288]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}