{"id":"https://openalex.org/W1577564952","doi":null,"title":"A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X-Y homologous gene amelogenin.","display_name":"A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X-Y homologous gene amelogenin.","publication_year":1993,"publication_date":"1993-10-01","ids":{"openalex":"https://openalex.org/W1577564952","mag":"1577564952","pmid":"https://pubmed.ncbi.nlm.nih.gov/8251166"},"language":"en","primary_location":{"id":"pmid:8251166","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8251166","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Technical Report"},"volume":null,"type":"report","is_traditional":false,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"K M Sullivan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K M Sullivan","raw_affiliation_strings":["Central Research and Support Establishment, Forensic Science Service, Berks, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research and Support Establishment, Forensic Science Service, Berks, UK","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070902312","display_name":"Armando Mannucci","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Mannucci","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071852469","display_name":"Colin P. Kimpton","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C P Kimpton","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5071387474","display_name":"Phillipa Gill","orcid":"https://orcid.org/0000-0003-4286-3018","observed_orcids":["https://orcid.org/0000-0003-4286-3018"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Gill","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":530,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"15","issue":"4","first_page":"636","last_page":"8, 640"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/amelogenin","display_name":"amelogenin","score":0.9629999995231628},{"id":"https://openalex.org/keywords/sex-chromosome-aneuploidy","display_name":"sex chromosome aneuploidy","score":0.8519999980926514},{"id":"https://openalex.org/keywords/microsatellite-markers","display_name":"microsatellite markers","score":0.699999988079071}],"concepts":[{"id":"https://openalex.org/C106434640","wikidata":"https://www.wikidata.org/wiki/Q2844797","display_name":"Amelogenin","level":3,"score":0.9576759934425354},{"id":"https://openalex.org/C2778271344","wikidata":"https://www.wikidata.org/wiki/Q903395","display_name":"Agarose gel electrophoresis","level":3,"score":0.6997753977775574},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.686104953289032},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6517210006713867},{"id":"https://openalex.org/C61320498","wikidata":"https://www.wikidata.org/wiki/Q265193","display_name":"Microsatellite","level":4,"score":0.5500539541244507},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.5443292856216431},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5367371439933777},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5333580374717712},{"id":"https://openalex.org/C64894306","wikidata":"https://www.wikidata.org/wiki/Q849622","display_name":"Homologous chromosome","level":3,"score":0.4931311309337616},{"id":"https://openalex.org/C2780246931","wikidata":"https://www.wikidata.org/wiki/Q202771","display_name":"Y chromosome","level":3,"score":0.4800754189491272},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4642767310142517},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.4638386368751526},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.45956140756607056},{"id":"https://openalex.org/C94671646","wikidata":"https://www.wikidata.org/wiki/Q207551","display_name":"Intron","level":3,"score":0.4142329692840576},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.41236862540245056},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.07517746090888977}],"mesh":[{"descriptor_ui":"D000782","descriptor_name":"Aneuploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003746","descriptor_name":"Dental Enamel Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004276","descriptor_name":"DNA, Satellite","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004587","descriptor_name":"Electrophoresis, Agar Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007438","descriptor_name":"Introns","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012732","descriptor_name":"Sex Determination Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053523","descriptor_name":"Amelogenin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:8251166","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8251166","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Technical Report"},{"id":"mag:1577564952","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8251166","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.428600013256073}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2087736065","https://openalex.org/W999856368","https://openalex.org/W187626228","https://openalex.org/W2272187332","https://openalex.org/W161746755","https://openalex.org/W2071066202","https://openalex.org/W1966808755","https://openalex.org/W2033146728","https://openalex.org/W2054666152","https://openalex.org/W2414464972"],"abstract_inverted_index":{"A":[0],"rapid,":[1],"simple":[2],"and":[3,39,46,71,103],"reliable":[4],"sex":[5,97],"test":[6,73],"that":[7,50,85],"entails":[8],"PCR":[9,41,83],"amplification":[10],"of":[11,14,28,32,63,81],"a":[12,25,120],"segment":[13],"the":[15,33,44,72,78,82,104],"X-Y":[16],"homologous":[17],"gene":[18],"amelogenin":[19,105],"has":[20,74],"been":[21,75],"developed.":[22],"We":[23],"used":[24],"single":[26],"pair":[27],"primers":[29],"spanning":[30],"part":[31],"first":[34],"intron":[35,106],"which":[36],"generated":[37],"106-bp":[38],"112-bp":[40],"products":[42,84],"from":[43],"X":[45],"Y":[47],"homologues,":[48],"respectively,":[49],"can":[51,108],"be":[52,101,110],"analyzed":[53],"simply":[54],"by":[55,77,91],"agarose":[56],"gel":[57],"electrophoresis.":[58],"Less":[59],"than":[60],"1":[61],"ng":[62],"template":[64],"DNA":[65,123],"is":[66],"required":[67],"for":[68],"gender":[69],"assignment,":[70],"automated":[76,92],"fluorescent":[79],"tagging":[80],"are":[86],"then":[87],"quantitated":[88],"during":[89],"electrophoresis":[90],"fluorescence-detection":[93],"technology.":[94],"Quantitation":[95],"enables":[96],"chromosome":[98],"aneuploidy":[99],"to":[100],"determined,":[102],"sequence":[107],"also":[109],"co-amplified":[111],"with":[112],"several":[113],"highly":[114],"polymorphic":[115],"microsatellite":[116],"loci,":[117],"thereby":[118],"providing":[119],"combined":[121],"gender/identity":[122],"test.":[124]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":9},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":18},{"year":2021,"cited_by_count":9},{"year":2020,"cited_by_count":13},{"year":2019,"cited_by_count":10},{"year":2018,"cited_by_count":12},{"year":2017,"cited_by_count":9},{"year":2016,"cited_by_count":12},{"year":2015,"cited_by_count":15},{"year":2014,"cited_by_count":22},{"year":2013,"cited_by_count":25},{"year":2012,"cited_by_count":23}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}