{"id":"https://openalex.org/W1575239368","doi":"https://doi.org/10.1111/j.1742-4658.2007.05959.x","title":"Analysis of the vacuolar luminal proteome of Saccharomyces cerevisiae","display_name":"Analysis of the vacuolar luminal proteome of Saccharomyces cerevisiae","publication_year":2007,"publication_date":"2007-07-25","ids":{"openalex":"https://openalex.org/W1575239368","doi":"https://doi.org/10.1111/j.1742-4658.2007.05959.x","mag":"1575239368","pmid":"https://pubmed.ncbi.nlm.nih.gov/17651441"},"language":"en","primary_location":{"id":"doi:10.1111/j.1742-4658.2007.05959.x","doi":"https://doi.org/10.1111/j.1742-4658.2007.05959.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1742-4658.2007.05959.x","pdf_url":null,"source":{"id":"https://openalex.org/S37672443","display_name":"FEBS Journal","issn_l":"1742-464X","issn":["1742-464X","1742-4658"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FEBS Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053533004","display_name":"Jean\u2010Emmanuel Sarry","orcid":"https://orcid.org/0000-0002-6704-2032","observed_orcids":["https://orcid.org/0000-0002-6704-2032"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jean\u2010Emmanuel Sarry","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA","Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","These authors contributed equally to this work"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"These authors contributed equally to this work","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089889431","display_name":"Sixue Chen","orcid":"https://orcid.org/0000-0002-6690-7612","observed_orcids":["https://orcid.org/0000-0002-6690-7612"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sixue Chen","raw_affiliation_strings":["Department of Botany, Genetics Institute, University of Florida, Gainesville, FL, USA","These authors contributed equally to this work"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Botany, Genetics Institute, University of Florida, Gainesville, FL, USA","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"These authors contributed equally to this work","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057126315","display_name":"Richard P. Collum","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard P. Collum","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005522488","display_name":"Shun Liang","orcid":"https://orcid.org/0000-0001-6503-7064","observed_orcids":["https://orcid.org/0000-0001-6503-7064"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shun Liang","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058634377","display_name":"Mingsheng Peng","orcid":"https://orcid.org/0009-0000-9906-9048","observed_orcids":["https://orcid.org/0009-0000-9906-9048"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mingsheng Peng","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112294715","display_name":"Albert Lang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Albert Lang","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034743620","display_name":"Bianca Naumann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bianca Naumann","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006795782","display_name":"Florence S. Dzierszinski","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Florence Dzierszinski","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112867858","display_name":"Chao\u2010Xing Yuan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chao\u2010Xing Yuan","raw_affiliation_strings":["Proteomics Core Facility, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Proteomics Core Facility, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079916943","display_name":"Michael Hippler","orcid":"https://orcid.org/0000-0001-9670-6101","observed_orcids":["https://orcid.org/0000-0001-9670-6101"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael Hippler","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109908855","display_name":"Philip A. Rea","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Philip A. Rea","raw_affiliation_strings":["Department of Biology, University of Pennsylvania, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pennsylvania, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":2.1603,"has_fulltext":false,"cited_by_count":47,"citation_normalized_percentile":{"value":0.85578792,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"274","issue":"16","first_page":"4287","last_page":"4305"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8359000086784363,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8359000086784363,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.1054999977350235,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vacuoles","display_name":"vacuoles","score":0.8889999985694885},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9610000252723694},{"id":"https://openalex.org/keywords/vacuolar-proteins","display_name":"vacuolar proteins","score":0.7519999742507935},{"id":"https://openalex.org/keywords/vacuolar-proteases","display_name":"vacuolar proteases","score":0.921999990940094},{"id":"https://openalex.org/keywords/glycoside-hydrolases","display_name":"glycoside hydrolases","score":0.8629999756813049},{"id":"https://openalex.org/keywords/phosphohydrolase","display_name":"phosphohydrolase","score":0.9089999794960022},{"id":"https://openalex.org/keywords/lipid-binding-proteins","display_name":"lipid-binding proteins","score":0.9649999737739563},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.6830000281333923},{"id":"https://openalex.org/keywords/protein-targeting","display_name":"protein targeting","score":0.4339999854564667},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.36899998784065247}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6964869499206543},{"id":"https://openalex.org/C102568950","wikidata":"https://www.wikidata.org/wiki/Q127702","display_name":"Vacuole","level":3,"score":0.655170738697052},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.6181744337081909},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.6012858152389526},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.5756469368934631},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.47636911273002625},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.46320095658302307},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4500935971736908},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.4172406494617462},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4021066427230835},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.34481436014175415},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.10729926824569702},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08685833215713501}],"mesh":[{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014617","descriptor_name":"Vacuoles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015180","descriptor_name":"Electrophoresis, Gel, Two-Dimensional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1742-4658.2007.05959.x","doi":"https://doi.org/10.1111/j.1742-4658.2007.05959.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1742-4658.2007.05959.x","pdf_url":null,"source":{"id":"https://openalex.org/S37672443","display_name":"FEBS Journal","issn_l":"1742-464X","issn":["1742-464X","1742-4658"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FEBS Journal","raw_type":"journal-article"},{"id":"pmid:17651441","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17651441","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FEBS journal","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/15","score":0.550000011920929,"display_name":"Life on land"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":67,"referenced_works":["https://openalex.org/W348360137","https://openalex.org/W1494307944","https://openalex.org/W1519979644","https://openalex.org/W1530873901","https://openalex.org/W1536172663","https://openalex.org/W1541928891","https://openalex.org/W1542314887","https://openalex.org/W1557954634","https://openalex.org/W1558468675","https://openalex.org/W1558697766","https://openalex.org/W1569582665","https://openalex.org/W1601358721","https://openalex.org/W1631725776","https://openalex.org/W1687563194","https://openalex.org/W1747506719","https://openalex.org/W1957349910","https://openalex.org/W1969416716","https://openalex.org/W1972962979","https://openalex.org/W1974858639","https://openalex.org/W1975605595","https://openalex.org/W1976390578","https://openalex.org/W1976979808","https://openalex.org/W1984241675","https://openalex.org/W1984315509","https://openalex.org/W1988926319","https://openalex.org/W1991556936","https://openalex.org/W1996286203","https://openalex.org/W2001642664","https://openalex.org/W2008201559","https://openalex.org/W2008735381","https://openalex.org/W2020087630","https://openalex.org/W2022704511","https://openalex.org/W2032467129","https://openalex.org/W2036794157","https://openalex.org/W2045850300","https://openalex.org/W2053376945","https://openalex.org/W2056026047","https://openalex.org/W2057663365","https://openalex.org/W2064699272","https://openalex.org/W2071231949","https://openalex.org/W2077419364","https://openalex.org/W2078626599","https://openalex.org/W2081037856","https://openalex.org/W2082202446","https://openalex.org/W2085694693","https://openalex.org/W2103036908","https://openalex.org/W2105137727","https://openalex.org/W2110764616","https://openalex.org/W2114736119","https://openalex.org/W2115312048","https://openalex.org/W2123144950","https://openalex.org/W2128635872","https://openalex.org/W2132367941","https://openalex.org/W2133437591","https://openalex.org/W2133790733","https://openalex.org/W2135951244","https://openalex.org/W2137690550","https://openalex.org/W2147652168","https://openalex.org/W2148146668","https://openalex.org/W2152243318","https://openalex.org/W2153283265","https://openalex.org/W2153665389","https://openalex.org/W2157937554","https://openalex.org/W2169357831","https://openalex.org/W2170678054","https://openalex.org/W2967175853","https://openalex.org/W3026061563"],"related_works":["https://openalex.org/W1983473744","https://openalex.org/W7098410057","https://openalex.org/W2127816774","https://openalex.org/W1540775618","https://openalex.org/W1986215321","https://openalex.org/W626172926","https://openalex.org/W4212925121","https://openalex.org/W2388924286","https://openalex.org/W4308145537","https://openalex.org/W1976979808"],"abstract_inverted_index":{"Despite":[0],"its":[1],"large":[2],"size":[3,177],"and":[4,38,86,93,109,114,147,152,178,257],"the":[5,14,17,20,24,49,60,66,69,87,124,128,182,196,234,240,243,247,250,254,258,268],"numerous":[6],"processes":[7],"in":[8,100,168,225],"which":[9,173],"it":[10],"is":[11],"implicated,":[12],"neither":[13],"identity":[15],"nor":[16],"functions":[18],"of":[19,51,59,71,89,105,111,127,172,181,195,211,236,242,246,253,260,267],"proteins":[21,73,197,206,210,221,223,255,271],"targeted":[22],"to":[23,33,41,79,156,175,190,275],"yeast":[25],"vacuole":[26],"have":[27,276],"been":[28],"defined":[29],"comprehensively.":[30],"In":[31],"order":[32],"establish":[34],"a":[35],"methodological":[36],"platform":[37],"protein":[39,107,228],"inventory":[40],"address":[42],"this":[43,77,101,117,169,278],"shortfall,":[44],"we":[45],"refined":[46],"techniques":[47],"for":[48,280],"purification":[50],"'proteomics-grade'":[52],"intact":[53],"vacuoles.":[54],"As":[55],"confirmed":[56],"by":[57,81,134,143,150],"retention":[58],"preloaded":[61],"fluorescent":[62],"conjugate":[63],"glutathione-bimane":[64],"throughout":[65],"fractionation":[67],"procedure,":[68],"resistance":[70],"soluble":[72,125],"that":[74],"copurify":[75],"with":[76,123],"fraction":[78,126],"digestion":[80],"exogenous":[82],"extravacuolar":[83],"proteinase":[84,262],"K,":[85],"results":[88,259],"flow":[90],"cytometric,":[91],"western":[92],"marker":[94],"enzyme":[95],"activity":[96],"analyses,":[97],"vacuoles":[98],"prepared":[99],"way":[102],"retain":[103],"most":[104],"their":[106],"content":[108],"are":[110,199,273],"high":[112,244],"purity":[113,245],"integrity.":[115],"Using":[116],"material,":[118],"360":[119],"polypeptides":[120,166],"species":[121],"associated":[122],"vacuolar":[129,201,209,248,270],"isolates":[130],"were":[131,141],"resolved":[132],"reproducibly":[133],"2D":[135],"gel":[136],"electrophoresis.":[137],"Of":[138],"these,":[139],"260":[140],"identified":[142,167,198,256,272],"peptide":[144,148],"mass":[145],"fingerprinting":[146],"sequencing":[149],"MALDI-MS":[151],"liquid":[153],"chromatography":[154],"coupled":[155],"ion":[157],"trap":[158],"or":[159,207,214,222,231,233],"quadrupole":[160],"TOF":[161],"tandem":[162],"MS,":[163],"respectively.":[164],"The":[165],"way,":[170],"many":[171,266],"correspond":[174],"alternate":[176],"charge":[179],"states":[180],"same":[183],"parent":[184],"translation":[185],"product,":[186],"can":[187],"be":[188],"assigned":[189],"117":[191],"unique":[192],"ORFs.":[193],"Most":[194],"canonical":[200],"proteases,":[202,216],"glycosidases,":[203,217],"phosphohydrolases,":[204],"lipid-binding":[205,218],"established":[208],"unknown":[212],"function,":[213],"other":[215],"proteins,":[219],"regulatory":[220],"involved":[224],"intermediary":[226],"metabolism,":[227],"synthesis,":[229],"folding":[230],"targeting,":[232],"alleviation":[235],"oxidative":[237],"stress.":[238],"On":[239],"basis":[241],"preparations,":[249],"electrophoretic":[251],"properties":[252],"quantitative":[261],"K":[263],"protection":[264],"measurements,":[265],"noncanonical":[269],"concluded":[274],"entered":[277],"compartment":[279],"breakdown,":[281],"processing":[282],"and/or":[283],"salvage":[284],"purposes.":[285]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}