{"id":"https://openalex.org/W1574422260","doi":"https://doi.org/10.1016/s0021-9258(18)42030-3","title":"Organella-targeted expression of rat liver cytochrome P450c27 in yeast. Genetically engineered alteration of mitochondrial P450 into a microsomal form creates a novel functional electron transport chain.","display_name":"Organella-targeted expression of rat liver cytochrome P450c27 in yeast. Genetically engineered alteration of mitochondrial P450 into a microsomal form creates a novel functional electron transport chain.","publication_year":1992,"publication_date":"1992-08-01","ids":{"openalex":"https://openalex.org/W1574422260","doi":"https://doi.org/10.1016/s0021-9258(18)42030-3","mag":"1574422260","pmid":"https://pubmed.ncbi.nlm.nih.gov/1322905"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)42030-3","doi":"https://doi.org/10.1016/s0021-9258(18)42030-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)42030-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)42030-3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5036846494","display_name":"Toshiyuki Sakaki","orcid":"https://orcid.org/0000-0001-7064-8185","observed_orcids":["https://orcid.org/0000-0001-7064-8185"]},"institutions":[{"id":"https://openalex.org/I139235422","display_name":"Sumitomo Chemical (Japan)","ror":"https://ror.org/03nyxmy82","country_code":"JP","type":"company","lineage":["https://openalex.org/I139235422"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"T Sakaki","raw_affiliation_strings":["Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan","institution_ids":["https://openalex.org/I139235422"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062265452","display_name":"Megumi Akiyoshi\u2010Shibata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139235422","display_name":"Sumitomo Chemical (Japan)","ror":"https://ror.org/03nyxmy82","country_code":"JP","type":"company","lineage":["https://openalex.org/I139235422"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"M Akiyoshi-Shibata","raw_affiliation_strings":["Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan","institution_ids":["https://openalex.org/I139235422"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056859828","display_name":"Yoshiyasu Yabusaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139235422","display_name":"Sumitomo Chemical (Japan)","ror":"https://ror.org/03nyxmy82","country_code":"JP","type":"company","lineage":["https://openalex.org/I139235422"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Y Yabusaki","raw_affiliation_strings":["Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan","institution_ids":["https://openalex.org/I139235422"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111472344","display_name":"Hideo Ohkawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139235422","display_name":"Sumitomo Chemical (Japan)","ror":"https://ror.org/03nyxmy82","country_code":"JP","type":"company","lineage":["https://openalex.org/I139235422"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H Ohkawa","raw_affiliation_strings":["Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co, Ltd., Hyogo, Japan","institution_ids":["https://openalex.org/I139235422"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I139235422"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.0057,"has_fulltext":false,"cited_by_count":62,"citation_normalized_percentile":{"value":0.72675053,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"267","issue":"23","first_page":"16497","last_page":"16502"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.698199987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.698199987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.06289999932050705,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.04800000041723251,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mitochondrial-targeting-signal","display_name":"mitochondrial targeting signal","score":0.5640000104904175},{"id":"https://openalex.org/keywords/cytochrome-p450c17","display_name":"P450c17","score":0.8809999823570251},{"id":"https://openalex.org/keywords/adrenodoxin","display_name":"adrenodoxin","score":0.7749999761581421},{"id":"https://openalex.org/keywords/electron-transport-chain","display_name":"electron transport chain","score":0.7839999794960022},{"id":"https://openalex.org/keywords/monooxygenase-activity","display_name":"monooxygenase activity","score":0.7940000295639038},{"id":"https://openalex.org/keywords/subcellular-targeting","display_name":"subcellular targeting","score":0.6230000257492065}],"concepts":[{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.7291033267974854},{"id":"https://openalex.org/C24840226","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Electron transport chain","level":2,"score":0.6561134457588196},{"id":"https://openalex.org/C526171541","wikidata":"https://www.wikidata.org/wiki/Q407693","display_name":"Cytochrome P450","level":3,"score":0.6346319913864136},{"id":"https://openalex.org/C87644729","wikidata":"https://www.wikidata.org/wiki/Q547502","display_name":"Microsome","level":3,"score":0.5716593861579895},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46804356575012207},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.467350035905838},{"id":"https://openalex.org/C2780768313","wikidata":"https://www.wikidata.org/wiki/Q408068","display_name":"Cytochrome","level":3,"score":0.41077521443367004},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39216065406799316},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2266508936882019}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002845","descriptor_name":"Chromatography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002848","descriptor_name":"Chromatography, DEAE-Cellulose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003577","descriptor_name":"Cytochrome P-450 Enzyme System","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D003577","descriptor_name":"Cytochrome P-450 Enzyme System","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D003577","descriptor_name":"Cytochrome P-450 Enzyme System","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004579","descriptor_name":"Electron Transport","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D006882","descriptor_name":"Hydroxyapatites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007106","descriptor_name":"Immune Sera","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008861","descriptor_name":"Microsomes","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008930","descriptor_name":"Mitochondria, Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015388","descriptor_name":"Organelles","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D017886","descriptor_name":"Durapatite","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)42030-3","doi":"https://doi.org/10.1016/s0021-9258(18)42030-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)42030-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:1322905","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1322905","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)42030-3","doi":"https://doi.org/10.1016/s0021-9258(18)42030-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)42030-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4000000059604645,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W1528427127","https://openalex.org/W1592724456","https://openalex.org/W1600617264","https://openalex.org/W1646275491","https://openalex.org/W1674435604","https://openalex.org/W1722287580","https://openalex.org/W1775749144","https://openalex.org/W1776107594","https://openalex.org/W1951469402","https://openalex.org/W1966183056","https://openalex.org/W1972737942","https://openalex.org/W1980829113","https://openalex.org/W1988201306","https://openalex.org/W1994803993","https://openalex.org/W2038556205","https://openalex.org/W2045364028","https://openalex.org/W2047736809","https://openalex.org/W2048952309","https://openalex.org/W2052690462","https://openalex.org/W2070107457","https://openalex.org/W2080177098","https://openalex.org/W2091306392","https://openalex.org/W2092191147","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2111661532","https://openalex.org/W2162240767","https://openalex.org/W2168350983"],"related_works":["https://openalex.org/W2043718821","https://openalex.org/W2328861146","https://openalex.org/W1977190397","https://openalex.org/W66975578","https://openalex.org/W2318125741","https://openalex.org/W2495394757","https://openalex.org/W2100159330","https://openalex.org/W2053337229","https://openalex.org/W2107329372","https://openalex.org/W1850243065"],"abstract_inverted_index":{"A":[0],"modified":[1,27,115],"rat":[2],"cytochrome":[3,20],"P450c27,":[4],"whose":[5],"mitochondrial":[6,61],"targeting":[7,16,62,110],"signal":[8,17,63],"had":[9],"been":[10,164],"replaced":[11],"by":[12,42],"a":[13,155],"possible":[14],"microsomal":[15],"of":[18,44,96,125,142,158],"bovine":[19,45,126],"P450c17,":[21],"was":[22,30,64,117],"expressed":[23,119],"in":[24,103,120,146,172],"yeast.":[25,173],"The":[26,130],"P450c27":[28,57,97,116],"hemoprotein":[29],"correctly":[31],"localized":[32],"on":[33,107,136],"yeast":[34,67,104,121,132],"microsomes":[35,102,138,171],"and":[36,48,81,128,139,144,149,169],"exhibited":[37],"the":[38,53,94,108,114,134,137,147,151,167,170],"P450c27-dependent":[39],"monooxygenase":[40,152],"activity":[41],"addition":[43],"adrenodoxin":[46],"(ADX)":[47],"NADPH-adrenodoxin":[49],"reductase":[50],"(ADR).":[51],"Considering":[52],"previous":[54],"observation":[55],"that":[56,93],"with":[58,122],"its":[59],"own":[60],"imported":[65],"into":[66],"mitochondria":[68,100],"(Akiyoshi-Shibata,":[69],"M.,":[70,78],"Usui,":[71],"E.,":[72],"Sakaki,":[73],"T.,":[74],"Yabusaki,":[75],"Y.,":[76],"Noshiro,":[77],"Okuda,":[79],"K.,":[80],"Ohkawa,":[82],"H.":[83],"(1991)":[84],"FEBS":[85],"Lett.":[86],"280,":[87],"367-370),":[88],"it":[89],"is":[90],"now":[91],"suggested":[92],"destination":[95],"to":[98],"either":[99],"or":[101],"depends":[105],"solely":[106],"amino-terminal":[109],"signal.":[111],"In":[112],"addition,":[113],"simultaneously":[118],"mature":[123,140],"forms":[124,141],"ADX":[127,143],"ADR.":[129],"recombinant":[131],"produced":[133],"P450":[135],"ADR":[145],"cytoplasm,":[148],"showed":[150],"activity.":[153],"Accordingly,":[154],"novel":[156],"type":[157],"functional":[159],"electron":[160],"transport":[161],"chain":[162],"has":[163],"established":[165],"between":[166],"cytoplasm":[168]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}