{"id":"https://openalex.org/W1571342896","doi":null,"title":"In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene.","display_name":"In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene.","publication_year":1997,"publication_date":"1997-02-01","ids":{"openalex":"https://openalex.org/W1571342896","mag":"1571342896","pmid":"https://pubmed.ncbi.nlm.nih.gov/9012474"},"language":"en","primary_location":{"id":"pmid:9012474","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9012474","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054807384","display_name":"Fumihiko Kanai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"F Kanai","raw_affiliation_strings":["Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077269939","display_name":"Keng\u2010Hsin Lan","orcid":"https://orcid.org/0000-0003-1781-0838","observed_orcids":["https://orcid.org/0000-0003-1781-0838"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K H Lan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113771422","display_name":"Yasushi Shiratori","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y Shiratori","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041636298","display_name":"Toshihiro Tanaka","orcid":"https://orcid.org/0000-0001-6201-9784","observed_orcids":["https://orcid.org/0000-0001-6201-9784"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Tanaka","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113438305","display_name":"Makoto Ohashi","orcid":"https://orcid.org/0009-0006-5134-6038","observed_orcids":["https://orcid.org/0009-0006-5134-6038"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Ohashi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112783364","display_name":"Takehito Okudaira","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Okudaira","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040884081","display_name":"Yoko Yoshida","orcid":"https://orcid.org/0000-0003-2837-4277","observed_orcids":["https://orcid.org/0000-0003-2837-4277"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y Yoshida","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006633218","display_name":"Hiroaki Wakimoto","orcid":"https://orcid.org/0000-0001-8225-241X","observed_orcids":["https://orcid.org/0000-0001-8225-241X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Wakimoto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109204206","display_name":"Hirohumi Hamada","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Hamada","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006366348","display_name":"Hidekazu Nakabayashi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Nakabayashi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077471426","display_name":"Tomoko Tamaoki","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Tamaoki","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5089860446","display_name":"Masao Omata","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Omata","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":8.8012,"has_fulltext":false,"cited_by_count":171,"citation_normalized_percentile":{"value":0.98572111,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"57","issue":"3","first_page":"461","last_page":"5"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12996","display_name":"Bacterial Cancer Therapy","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10073","display_name":"Hepatocellular Carcinoma Treatment and Prognosis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepatocellular-carcinoma","display_name":"hepatocellular carcinoma","score":0.878000020980835},{"id":"https://openalex.org/keywords/alpha-fetoprotein","display_name":"alpha-fetoprotein","score":0.8920000195503235},{"id":"https://openalex.org/keywords/gene-therapy","display_name":"gene therapy","score":0.734000027179718},{"id":"https://openalex.org/keywords/adenovirus-vectors","display_name":"adenoviral vector","score":0.8700000047683716},{"id":"https://openalex.org/keywords/cytosine-deaminase","display_name":"cytosine deaminase","score":0.9929999709129333},{"id":"https://openalex.org/keywords/suicide-gene","display_name":"suicide gene","score":0.8519999980926514},{"id":"https://openalex.org/keywords/5-fluorocytosine","display_name":"5-fluorocytosine","score":0.9879999756813049},{"id":"https://openalex.org/keywords/beta-galactosidase","display_name":"beta-galactosidase","score":0.7799999713897705},{"id":"https://openalex.org/keywords/tumor-xenografts","display_name":"tumor xenografts","score":0.7720000147819519},{"id":"https://openalex.org/keywords/selective-gene-expression","display_name":"selective gene expression","score":0.6769999861717224},{"id":"https://openalex.org/keywords/tumor-regression","display_name":"tumor regression","score":0.6629999876022339}],"concepts":[{"id":"https://openalex.org/C2777748644","wikidata":"https://www.wikidata.org/wiki/Q5201383","display_name":"Cytosine deaminase","level":4,"score":0.7885094881057739},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6535979509353638},{"id":"https://openalex.org/C111936080","wikidata":"https://www.wikidata.org/wiki/Q913367","display_name":"Enhancer","level":4,"score":0.6378427743911743},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6342595219612122},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.6178860068321228},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.5194777250289917},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.47925567626953125},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4643305838108063},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.42999985814094543},{"id":"https://openalex.org/C2780624289","wikidata":"https://www.wikidata.org/wiki/Q306076","display_name":"Alpha-fetoprotein","level":3,"score":0.42961329221725464},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.42295193672180176},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.4154640734195709},{"id":"https://openalex.org/C2776954459","wikidata":"https://www.wikidata.org/wiki/Q193447","display_name":"Adenoviridae","level":4,"score":0.41523921489715576},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3841540217399597},{"id":"https://openalex.org/C2778019345","wikidata":"https://www.wikidata.org/wiki/Q1148337","display_name":"Hepatocellular carcinoma","level":2,"score":0.32432234287261963},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.1629193127155304},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.05599531531333923},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000509","descriptor_name":"alpha-Fetoproteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006528","descriptor_name":"Carcinoma, Hepatocellular","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D006528","descriptor_name":"Carcinoma, Hepatocellular","qualifier_ui":"Q000628","qualifier_name":"therapy","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008113","descriptor_name":"Liver Neoplasms","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008113","descriptor_name":"Liver Neoplasms","qualifier_ui":"Q000628","qualifier_name":"therapy","is_major_topic":true},{"descriptor_ui":"D008807","descriptor_name":"Mice, Inbred BALB C","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009368","descriptor_name":"Neoplasm Transplantation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009700","descriptor_name":"Nucleoside Deaminases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D043525","descriptor_name":"Cytosine Deaminase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:9012474","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9012474","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},{"id":"mag:1571342896","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9012474","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9940000176429749}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2062975876","https://openalex.org/W1561100829","https://openalex.org/W4249512585","https://openalex.org/W1484397181","https://openalex.org/W2126928946","https://openalex.org/W2021860423","https://openalex.org/W2165301694","https://openalex.org/W2121030043","https://openalex.org/W202556135","https://openalex.org/W875510739"],"abstract_inverted_index":{"The":[0],"alpha-fetoprotein":[1],"(AFP)":[2],"gene":[3,49,54,65,101,127,170],"is":[4,11],"normally":[5],"expressed":[6],"in":[7,14,19,56,79,85,108,121,147,158,171,175,178],"fetal":[8],"liver":[9,16],"and":[10,51,99,105,142,177],"transcriptionally":[12],"silent":[13],"adult":[15],"but":[17,83],"overexpressed":[18],"human":[20,35],"hepatocellular":[21,172],"carcinoma":[22,173],"(HCC).":[23],"Here,":[24],"we":[25],"demonstrate":[26],"that":[27,152],"replication":[28],"defective":[29],"recombinant":[30],"adenoviral":[31,92,160],"vectors,":[32],"containing":[33],"the":[34,43,52,63,69,80,86,117,125,155],"AFP":[36,70,156],"promoter/enhancer,":[37],"can":[38,162],"be":[39],"used":[40],"to":[41,76,130],"express":[42],"Escherichia":[44],"coli":[45],"cytosine":[46],"deaminase":[47],"(CD)":[48],"(AdAFPCD)":[50,72],"beta-galactosidase":[53,126],"(AdAF-PlacZ)":[55],"AFP-producing":[57,81,131],"HCC":[58,132,135],"cell":[59,110],"lines.":[60,111],"Expression":[61],"of":[62,124,154,166],"CD":[64,100],"by":[66,90,138],"adenovirus":[67],"from":[68],"promoter/enhancer":[71,157],"induced":[73],"cells":[74,82,174],"sensitive":[75],"5-fluorocytosine":[77],"(5FC)":[78],"not":[84],"AFP-nonproducing":[87],"cells.":[88],"Transduction":[89],"an":[91,95,159],"vector":[93,161],"harboring":[94],"ubiquitous":[96],"strong":[97],"promoter":[98],"showed":[102],"enzymatic":[103],"activity":[104],"5FC":[106,145],"killing":[107],"all":[109],"When":[112],"AdAFPlacZ":[113],"was":[114,128],"injected":[115],"into":[116],"s.c.":[118],"established":[119],"hepatoma":[120],"vivo,":[122],"expression":[123,165],"confined":[129],"xenografts.":[133],"Moreover,":[134],"xenografts":[136],"regressed":[137],"transduction":[139],"with":[140,144],"AdAFPCD":[141],"subsequently":[143],"treatment":[146],"vivo.":[148,179],"These":[149],"findings":[150],"suggest":[151],"utilization":[153],"confer":[163],"selective":[164],"a":[167],"heterologous":[168],"suicide":[169],"vitro":[176]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}