{"id":"https://openalex.org/W1570089337","doi":"https://doi.org/10.1016/s0021-9258(19)57287-8","title":"The transition metal-mediated formation of the hydroxyl free radical during the reduction of molecular oxygen by ferredoxin-ferredoxin:NADP+ oxidoreductase.","display_name":"The transition metal-mediated formation of the hydroxyl free radical during the reduction of molecular oxygen by ferredoxin-ferredoxin:NADP+ oxidoreductase.","publication_year":1988,"publication_date":"1988-01-01","ids":{"openalex":"https://openalex.org/W1570089337","doi":"https://doi.org/10.1016/s0021-9258(19)57287-8","mag":"1570089337","pmid":"https://pubmed.ncbi.nlm.nih.gov/2826473"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)57287-8","doi":"https://doi.org/10.1016/s0021-9258(19)57287-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)57287-8","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)57287-8","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006412880","display_name":"Kim M. Morehouse","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"K M Morehouse","raw_affiliation_strings":["Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015414029","display_name":"Ronald Paul Mason","orcid":"https://orcid.org/0000-0002-1859-9109","observed_orcids":["https://orcid.org/0000-0002-1859-9109"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R P Mason","raw_affiliation_strings":["Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":15.6736,"has_fulltext":false,"cited_by_count":80,"citation_normalized_percentile":{"value":0.99726572,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"263","issue":"3","first_page":"1204","last_page":"1210"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.7958999872207642,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.7958999872207642,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.1324000060558319,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.029600000008940697,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydroxyl-radicals","display_name":"hydroxyl radicals","score":0.8429999947547913},{"id":"https://openalex.org/keywords/ferredoxin","display_name":"ferredoxin","score":0.8100000023841858},{"id":"https://openalex.org/keywords/nadph","display_name":"NADPH","score":0.6859999895095825},{"id":"https://openalex.org/keywords/oxygen-reduction-reaction","display_name":"oxygen reduction reaction","score":0.7319999933242798},{"id":"https://openalex.org/keywords/hydrogen-peroxide","display_name":"hydrogen peroxide","score":0.925000011920929},{"id":"https://openalex.org/keywords/fenton-reaction","display_name":"Fenton reaction","score":0.781000018119812},{"id":"https://openalex.org/keywords/transition-metal-chelation","display_name":"transition metal chelation","score":0.6830000281333923},{"id":"https://openalex.org/keywords/iron-reduction","display_name":"iron reduction","score":0.5789999961853027},{"id":"https://openalex.org/keywords/catalase-inhibition","display_name":"catalase inhibition","score":0.5479999780654907},{"id":"https://openalex.org/keywords/dmpo-spin-trapping","display_name":"DMPO spin trapping","score":0.652999997138977},{"id":"https://openalex.org/keywords/oxygen-centered-radicals","display_name":"oxygen-centered radicals","score":0.5360000133514404},{"id":"https://openalex.org/keywords/hydrogen-abstraction","display_name":"hydrogen abstraction","score":0.5090000033378601}],"concepts":[{"id":"https://openalex.org/C134621786","wikidata":"https://www.wikidata.org/wiki/Q415215","display_name":"Ferredoxin","level":3,"score":0.9749644994735718},{"id":"https://openalex.org/C2779268744","wikidata":"https://www.wikidata.org/wiki/Q407479","display_name":"Oxidoreductase","level":3,"score":0.7833395004272461},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6789419651031494},{"id":"https://openalex.org/C70412867","wikidata":"https://www.wikidata.org/wiki/Q5445278","display_name":"Ferredoxin-thioredoxin reductase","level":5,"score":0.5552131533622742},{"id":"https://openalex.org/C540031477","wikidata":"https://www.wikidata.org/wiki/Q629","display_name":"Oxygen","level":2,"score":0.5490890741348267},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.48962122201919556},{"id":"https://openalex.org/C2992754303","wikidata":"https://www.wikidata.org/wiki/Q1641506","display_name":"Oxygen reduction","level":4,"score":0.4735599756240845},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3279820680618286},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.26921117305755615},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.11896449327468872},{"id":"https://openalex.org/C52859227","wikidata":"https://www.wikidata.org/wiki/Q7877","display_name":"Electrochemistry","level":3,"score":0.06515130400657654},{"id":"https://openalex.org/C3623737","wikidata":"https://www.wikidata.org/wiki/Q421219","display_name":"Thioredoxin","level":3,"score":0.053336113691329956},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.05076587200164795},{"id":"https://openalex.org/C17525397","wikidata":"https://www.wikidata.org/wiki/Q176140","display_name":"Electrode","level":2,"score":0.0},{"id":"https://openalex.org/C2777737464","wikidata":"https://www.wikidata.org/wiki/Q2540636","display_name":"Thioredoxin reductase","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004578","descriptor_name":"Electron Spin Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005287","descriptor_name":"Ferredoxin-NADP Reductase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005288","descriptor_name":"Ferredoxins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006878","descriptor_name":"Hydroxides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008670","descriptor_name":"Metals","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009247","descriptor_name":"NADH, NADPH Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010100","descriptor_name":"Oxygen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017665","descriptor_name":"Hydroxyl Radical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)57287-8","doi":"https://doi.org/10.1016/s0021-9258(19)57287-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)57287-8","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2826473","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2826473","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)57287-8","doi":"https://doi.org/10.1016/s0021-9258(19)57287-8","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)57287-8","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.6499999761581421,"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":72,"referenced_works":["https://openalex.org/W241289362","https://openalex.org/W418119151","https://openalex.org/W627720834","https://openalex.org/W950616869","https://openalex.org/W1508450059","https://openalex.org/W1519219147","https://openalex.org/W1534586762","https://openalex.org/W1541881494","https://openalex.org/W1546482094","https://openalex.org/W1571521625","https://openalex.org/W1589413892","https://openalex.org/W1595733202","https://openalex.org/W1818575480","https://openalex.org/W1953847668","https://openalex.org/W1964162589","https://openalex.org/W1969899546","https://openalex.org/W1971047897","https://openalex.org/W1973771797","https://openalex.org/W1974808158","https://openalex.org/W1982774891","https://openalex.org/W1983851192","https://openalex.org/W1987805442","https://openalex.org/W1992648148","https://openalex.org/W1995340760","https://openalex.org/W1998819481","https://openalex.org/W2002769318","https://openalex.org/W2004073762","https://openalex.org/W2008355183","https://openalex.org/W2008503644","https://openalex.org/W2011141652","https://openalex.org/W2013157983","https://openalex.org/W2017772699","https://openalex.org/W2021973381","https://openalex.org/W2024138485","https://openalex.org/W2025305839","https://openalex.org/W2027403336","https://openalex.org/W2027994975","https://openalex.org/W2029637215","https://openalex.org/W2032056587","https://openalex.org/W2037355109","https://openalex.org/W2037877887","https://openalex.org/W2040687325","https://openalex.org/W2043330517","https://openalex.org/W2047202043","https://openalex.org/W2050994988","https://openalex.org/W2053111837","https://openalex.org/W2054192647","https://openalex.org/W2060034267","https://openalex.org/W2061778764","https://openalex.org/W2070201210","https://openalex.org/W2073758178","https://openalex.org/W2074365079","https://openalex.org/W2077191880","https://openalex.org/W2083048780","https://openalex.org/W2083944989","https://openalex.org/W2085215055","https://openalex.org/W2092699092","https://openalex.org/W2099086632","https://openalex.org/W2120909991","https://openalex.org/W2122324588","https://openalex.org/W2163946974","https://openalex.org/W2210619251","https://openalex.org/W2237766589","https://openalex.org/W2270686920","https://openalex.org/W2283573532","https://openalex.org/W2309733666","https://openalex.org/W2401979417","https://openalex.org/W2599525179","https://openalex.org/W4211129887","https://openalex.org/W4234604396","https://openalex.org/W4243681431","https://openalex.org/W4246149649"],"related_works":["https://openalex.org/W2029958015","https://openalex.org/W2070931690","https://openalex.org/W1983851192","https://openalex.org/W2132304605","https://openalex.org/W1984394276","https://openalex.org/W1789918588","https://openalex.org/W2058143492","https://openalex.org/W1964162589","https://openalex.org/W2063370374","https://openalex.org/W1968249505"],"abstract_inverted_index":{"The":[0,12,27,66,84,202],"NADPH-supported":[1],"enzymatic":[2,111],"reduction":[3,75,165,191,217,227],"of":[4,25,68,76,115,118,135,137,148,157,166,192,204,218,228],"molecular":[5,54],"oxygen":[6,77,193],"by":[7,221],"ferredoxin-ferredoxin:NADP+":[8],"oxidoreductase":[9,181],"was":[10,17,33,44,78,99,107,122,126,143],"investigated.":[11],"ESR":[13],"spin":[14,28],"trapping":[15],"technique":[16],"employed":[18,45],"to":[19,35,46,109,145,194,231],"identify":[20,38],"the":[21,39,49,57,59,69,74,94,110,116,119,130,133,138,149,155,164,190,199,209,216,226,232],"free":[22,41,70,97],"radical":[23,72,86,98,121,140,151,211,234],"metabolites":[24],"oxygen.":[26,55,167],"trap":[29,36],"5,5-dimethyl-1-pyrroline":[30],"N-oxide":[31],"(DMPO)":[32],"used":[34],"and":[37,93,132,174,197],"oxygen-derived":[40],"radicals.":[42],"[17O]Oxygen":[43],"demonstrate":[47],"that":[48,215],"oxygen-centered":[50],"radicals":[51],"arose":[52],"from":[53,89],"From":[56],"data,":[58],"following":[60],"scheme":[61],"is":[62,187,223],"proposed:":[63],"(Formula:see":[64],"text).":[65],"formation":[67,136,147,156],"hydroxyl":[71,85,120,150,200,233],"during":[73,163],"demonstrated":[79],"with":[80,101],"quantitative":[81],"competition":[82],"experiments.":[83],"abstracted":[87],"hydrogen":[88,158,195,229],"ethanol":[90],"or":[91],"formate,":[92],"resulting":[95],"scavenger-derived":[96],"trapped":[100],"known":[102],"rate":[103,134],"constants.":[104],"If":[105],"H2O2":[106],"added":[108],"reaction,":[112],"a":[113],"stimulation":[114,125],"production":[117],"obtained.":[123],"This":[124],"manifested":[127],"in":[128,185],"both":[129],"concentration":[131,213],"DMPO/hydroxyl":[139,210],"adduct.":[141],"Catalase":[142],"shown":[144],"inhibit":[146],"adduct,":[152],"further":[153],"supporting":[154],"peroxide":[159,196,230],"as":[160],"an":[161],"intermediate":[162],"All":[168],"three":[169],"components,":[170],"ferredoxin,":[171,183],"ferredoxin:NADP+":[172],"oxidoreductase,":[173],"NADPH,":[175],"were":[176],"required":[177],"for":[178,189,225],"reduction.":[179],"Ferredoxin:NADP+":[180],"reduces":[182],"which":[184],"turn":[186],"responsible":[188,224],"ultimately":[198],"radical.":[201],"effect":[203],"transition":[205],"metal":[206],"chelators":[207],"on":[208],"adduct":[212],"suggests":[214],"chelated":[219],"iron":[220],"ferredoxin":[222],"via":[235],"Fenton-type":[236],"chemistry.":[237]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}