{"id":"https://openalex.org/W1567744708","doi":null,"title":"GROUP I INTRON-DERIVED RIBOZYME REACTIONS","display_name":"GROUP I INTRON-DERIVED RIBOZYME REACTIONS","publication_year":2005,"publication_date":"2005-01-01","ids":{"openalex":"https://openalex.org/W1567744708","mag":"1567744708"},"language":"en","primary_location":{"id":"pmh:oai:uknowledge.uky.edu:gradschool_diss-1289","doi":null,"is_oa":false,"landing_page_url":"https://uknowledge.uky.edu/gradschool_diss/286","pdf_url":null,"source":{"id":"https://openalex.org/S4306402623","display_name":"UKnowledge (University of Kentucky)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I143302722","host_organization_name":"University of Kentucky","host_organization_lineage":["https://openalex.org/I143302722"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"University of Kentucky Doctoral Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082583940","display_name":"Ashley K. Johnson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ashley K. Johnson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5082583940"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.8715999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.8715999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.04430000111460686,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.023399999365210533,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/group-i-introns","display_name":"group I introns","score":0.9739999771118164},{"id":"https://openalex.org/keywords/ribozymes","display_name":"ribozymes","score":0.8769999742507935},{"id":"https://openalex.org/keywords/suicide-inhibition","display_name":"suicide inhibition","score":0.8130000233650208},{"id":"https://openalex.org/keywords/cyclization","display_name":"cyclization","score":0.8840000033378601},{"id":"https://openalex.org/keywords/molecular-recognition","display_name":"molecular recognition","score":0.6809999942779541},{"id":"https://openalex.org/keywords/pneumocystis-carinii","display_name":"Pneumocystis carinii","score":0.9359999895095825},{"id":"https://openalex.org/keywords/tetrahymena-thermophila","display_name":"Tetrahymena thermophila","score":0.9990000128746033},{"id":"https://openalex.org/keywords/rna-tertiary-structure","display_name":"RNA tertiary structure","score":0.4650000035762787},{"id":"https://openalex.org/keywords/mismatch-tolerance","display_name":"mismatch tolerance","score":0.6290000081062317}],"concepts":[{"id":"https://openalex.org/C64962909","wikidata":"https://www.wikidata.org/wiki/Q5611160","display_name":"Group II intron","level":5,"score":0.7764427065849304},{"id":"https://openalex.org/C76346623","wikidata":"https://www.wikidata.org/wiki/Q205858","display_name":"Ribozyme","level":4,"score":0.7098269462585449},{"id":"https://openalex.org/C94671646","wikidata":"https://www.wikidata.org/wiki/Q207551","display_name":"Intron","level":3,"score":0.6351833343505859},{"id":"https://openalex.org/C2781311116","wikidata":"https://www.wikidata.org/wiki/Q83306","display_name":"Group (periodic table)","level":2,"score":0.6070252060890198},{"id":"https://openalex.org/C118346598","wikidata":"https://www.wikidata.org/wiki/Q5611164","display_name":"Group I catalytic intron","level":5,"score":0.5327423214912415},{"id":"https://openalex.org/C30951146","wikidata":"https://www.wikidata.org/wiki/Q6745754","display_name":"Mammalian CPEB3 ribozyme","level":5,"score":0.48355433344841003},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3765261769294739},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3745509684085846},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.30773842334747314},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.29736173152923584},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.253152459859848},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19128000736236572},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:uknowledge.uky.edu:gradschool_diss-1289","doi":null,"is_oa":false,"landing_page_url":"https://uknowledge.uky.edu/gradschool_diss/286","pdf_url":null,"source":{"id":"https://openalex.org/S4306402623","display_name":"UKnowledge (University of Kentucky)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I143302722","host_organization_name":"University of Kentucky","host_organization_lineage":["https://openalex.org/I143302722"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"University of Kentucky Doctoral Dissertations","raw_type":"text"},{"id":"mag:1567744708","doi":null,"is_oa":false,"landing_page_url":"https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1289&context=gradschool_diss","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":86,"referenced_works":["https://openalex.org/W214392","https://openalex.org/W1494641947","https://openalex.org/W1647075334","https://openalex.org/W1849580424","https://openalex.org/W1964927838","https://openalex.org/W1965174422","https://openalex.org/W1968282235","https://openalex.org/W1969292324","https://openalex.org/W1973259045","https://openalex.org/W1975613162","https://openalex.org/W1975653587","https://openalex.org/W1977698865","https://openalex.org/W1979496015","https://openalex.org/W1980410097","https://openalex.org/W1982190757","https://openalex.org/W1982825221","https://openalex.org/W1985919704","https://openalex.org/W1986489741","https://openalex.org/W1987300969","https://openalex.org/W1988071813","https://openalex.org/W1989200332","https://openalex.org/W1994223718","https://openalex.org/W1995767443","https://openalex.org/W1996967245","https://openalex.org/W1997420824","https://openalex.org/W1998020149","https://openalex.org/W1999404678","https://openalex.org/W2000352671","https://openalex.org/W2000790953","https://openalex.org/W2003698664","https://openalex.org/W2005665960","https://openalex.org/W2006144062","https://openalex.org/W2007404424","https://openalex.org/W2012555497","https://openalex.org/W2013319637","https://openalex.org/W2018713874","https://openalex.org/W2021333801","https://openalex.org/W2021693909","https://openalex.org/W2021955952","https://openalex.org/W2022449135","https://openalex.org/W2031931648","https://openalex.org/W2032363898","https://openalex.org/W2038730272","https://openalex.org/W2042229053","https://openalex.org/W2042332671","https://openalex.org/W2042841971","https://openalex.org/W2043094833","https://openalex.org/W2046676768","https://openalex.org/W2050146223","https://openalex.org/W2053252723","https://openalex.org/W2055214702","https://openalex.org/W2056171984","https://openalex.org/W2060510537","https://openalex.org/W2061357866","https://openalex.org/W2064846797","https://openalex.org/W2065262709","https://openalex.org/W2065324886","https://openalex.org/W2065596245","https://openalex.org/W2069115217","https://openalex.org/W2069668894","https://openalex.org/W2071929273","https://openalex.org/W2073966309","https://openalex.org/W2076499829","https://openalex.org/W2076857736","https://openalex.org/W2077267518","https://openalex.org/W2085272777","https://openalex.org/W2085504720","https://openalex.org/W2096973761","https://openalex.org/W2102575752","https://openalex.org/W2102915071","https://openalex.org/W2103670964","https://openalex.org/W2115986135","https://openalex.org/W2116463701","https://openalex.org/W2134816300","https://openalex.org/W2141573080","https://openalex.org/W2154989771","https://openalex.org/W2157147361","https://openalex.org/W2158654777","https://openalex.org/W2158875436","https://openalex.org/W2160127977","https://openalex.org/W2161827663","https://openalex.org/W2163414819","https://openalex.org/W2165810116","https://openalex.org/W2166887868","https://openalex.org/W2169544012","https://openalex.org/W2169751581"],"related_works":["https://openalex.org/W2094122355","https://openalex.org/W1987300969","https://openalex.org/W2106101135","https://openalex.org/W1493769649","https://openalex.org/W1580909586","https://openalex.org/W7099006172","https://openalex.org/W1998020149","https://openalex.org/W2047774680","https://openalex.org/W2078660905","https://openalex.org/W2038730272"],"abstract_inverted_index":{"Group":[0],"I":[1,18,59,105,152,190,285],"introns":[2,19],"are":[3,20,35],"catalytic":[4],"RNAs":[5],"capable":[6],"of":[7,10,42,57,82,93,119,147,250,298],"self-splicing":[8],"out":[9],"RNA":[11,132,166,234],"transcripts.":[12],"Ribozymes":[13],"derived":[14,192],"from":[15,107,155,194],"these":[16,43,120],"group":[17,58,104,151,189,284],"used":[21],"to":[22,45,87,144,162,304],"explore":[23],"the":[24,39,53,77,89,108,116,145,175,195,202,212,227,241,247,251,255,262,305],"molecular":[25,54,70,274],"recognition":[26,55,71,275],"properties":[27,56,72],"involved":[28],"in":[29,283],"intron":[30,106,191],"catalysis.":[31],"New":[32],"ribozyme":[33,61,67,154,193,221,230,266,287],"reactions":[34,62,100,126],"designed":[36],"based":[37],"on":[38],"inherent":[40],"ability":[41],"ribozymes":[44],"perform":[46,201],"site-specific":[47],"nucleophilic":[48],"attacks.":[49],"This":[50],"study":[51],"explores":[52],"intron-derived":[60,153,286],"and":[63,80,97,172,236],"describe":[64],"a":[65,83,103,188,219],"new":[66,84,220,294],"reaction":[68,222,245,258,295],"involving":[69],"previously":[73,160,281],"not":[74,128,207,260],"seen.We":[75],"report":[76,186],"development,":[78],"analysis,":[79],"use":[81],"combinatorial":[85],"approach":[86],"analyze":[88],"substrate":[90],"sequence":[91,117],"dependence":[92],"suicide":[94],"inhibition,":[95],"cyclization,":[96],"reverse":[98,248,263],"cyclization":[99],"catalyzed":[101],"by":[102],"opportunistic":[109],"pathogen":[110],"Pneumocystis":[111],"carinii.":[112],"We":[113,184,289],"demonstrate":[114],"that":[115,131,187],"specificity":[118,142],"Internal":[121],"Guide":[122],"Sequence":[123],"(IGS)":[124],"mediated":[125],"is":[127,259],"high,":[129],"suggesting":[130],"targeting":[133],"strategies":[134],"which":[135],"exploit":[136],"tertiary":[137],"interactions":[138,276],"could":[139],"have":[140],"low":[141],"due":[143],"tolerance":[146],"mismatched":[148],"base":[149],"pairs.A":[150],"P.":[156,213,228],"carinii":[157,214,229],"has":[158,279],"been":[159,280],"shown":[161],"bind":[163],"an":[164,169],"exogenous":[165,233,269],"substrate,":[167],"splice-out":[168],"internal":[170],"segment,":[171],"then":[173],"ligate":[174],"two":[176,232,268],"ends":[177],"back":[178],"together":[179],"(the":[180],"trans":[181,203,224,252,256],"excision-splicing":[182,204,253],"reaction).":[183],"now":[185],"ciliate":[196],"Tetrahymena":[197],"thermophila":[198],"can":[199],"also":[200],"reaction,":[205,254],"although":[206],"nearly":[208],"as":[209,211],"well":[210],"ribozyme.In":[215],"addition,":[216],"we":[217],"discovered":[218],"called":[223],"insertion-splicing":[225,257],"where":[226],"binds":[231],"substrates":[235,270],"inserts":[237],"one":[238],"directly":[239],"into":[240],"other.":[242],"Although":[243],"this":[244,293],"gives":[246],"products":[249],"simply":[261],"reaction.":[264],"The":[265],"recognizes":[267],"through":[271],"more":[272],"complex":[273],"than":[277],"what":[278],"seen":[282],"reactions.":[288],"give":[290],"evidence":[291],"for":[292],"mechanism":[296],"composed":[297],"three":[299],"steps,":[300],"with":[301],"intermediates":[302],"attached":[303],"ribozyme.":[306]},"counts_by_year":[],"updated_date":"2026-08-18T07:49:30.821534","created_date":"2016-06-24T00:00:00"}
