{"id":"https://openalex.org/W1567006352","doi":null,"title":"Alteration of ocular surface mucins in MUC5AC-DTA transgenic mice.","display_name":"Alteration of ocular surface mucins in MUC5AC-DTA transgenic mice.","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W1567006352","mag":"1567006352","pmid":"https://pubmed.ncbi.nlm.nih.gov/19158956","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2628312"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2628312","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2628312","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Vis","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2628312","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046520432","display_name":"I\u2010Jong Wang","orcid":"https://orcid.org/0000-0002-3045-0614","observed_orcids":["https://orcid.org/0000-0002-3045-0614"]},"institutions":[{"id":"https://openalex.org/I4210131804","display_name":"National Taiwan University Hospital","ror":"https://ror.org/03nteze27","country_code":"TW","type":"healthcare","lineage":["https://openalex.org/I4210131804"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"I-Jong Wang","raw_affiliation_strings":["Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan","institution_ids":["https://openalex.org/I4210131804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013389671","display_name":"Chong\u2010Jen Yu","orcid":"https://orcid.org/0000-0001-5664-9392","observed_orcids":["https://orcid.org/0000-0001-5664-9392"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chong-Jen Yu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5053657893","display_name":"Fung\u2010Rong Hu","orcid":"https://orcid.org/0000-0002-2102-5317","observed_orcids":["https://orcid.org/0000-0002-2102-5317"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Fung-Rong Hu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.6399,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.70999733,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"15","issue":null,"first_page":"108","last_page":"19"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10997","display_name":"Ocular Surface and Contact Lens","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10997","display_name":"Ocular Surface and Contact Lens","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10920","display_name":"Advanced Drug Delivery Systems","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/3003","display_name":"Pharmaceutical Science"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/muc5ac","display_name":"MUC5AC","score":0.8539999723434448},{"id":"https://openalex.org/keywords/muc4","display_name":"MUC4","score":0.9919999837875366},{"id":"https://openalex.org/keywords/conjunctival-goblet-cells","display_name":"conjunctival goblet cells","score":0.8050000071525574},{"id":"https://openalex.org/keywords/secretory-mucins","display_name":"secretory mucins","score":0.7319999933242798},{"id":"https://openalex.org/keywords/transgenic-mice","display_name":"transgenic mice","score":0.7839999794960022}],"concepts":[{"id":"https://openalex.org/C179264091","wikidata":"https://www.wikidata.org/wiki/Q410862","display_name":"Mucin","level":2,"score":0.9697422385215759},{"id":"https://openalex.org/C2775941134","wikidata":"https://www.wikidata.org/wiki/Q813622","display_name":"Goblet cell","level":3,"score":0.7063202261924744},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.5830186009407043},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5801541209220886},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.568354070186615},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.4928872585296631},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4540650248527527},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43902266025543213},{"id":"https://openalex.org/C2777312293","wikidata":"https://www.wikidata.org/wiki/Q3649349","display_name":"Mucus","level":2,"score":0.41497477889060974},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.4097170829772949},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.27270495891571045},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.21918243169784546},{"id":"https://openalex.org/C529295009","wikidata":"https://www.wikidata.org/wiki/Q41301","display_name":"Epithelium","level":2,"score":0.19125774502754211},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10902440547943115},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10370197892189026},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07830491662025452},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.05148458480834961},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003228","descriptor_name":"Conjunctiva","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D003228","descriptor_name":"Conjunctiva","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003228","descriptor_name":"Conjunctiva","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D004167","descriptor_name":"Diphtheria Toxin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004167","descriptor_name":"Diphtheria Toxin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005123","descriptor_name":"Eye","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D005123","descriptor_name":"Eye","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008822","descriptor_name":"Mice, Transgenic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009928","descriptor_name":"Organ Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015139","descriptor_name":"Blotting, Southern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018628","descriptor_name":"Gene Dosage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020287","descriptor_name":"In Situ Nick-End Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020397","descriptor_name":"Goblet Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D020397","descriptor_name":"Goblet Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D022142","descriptor_name":"Secretory Vesicles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D022142","descriptor_name":"Secretory Vesicles","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055264","descriptor_name":"Mucin-4","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D055264","descriptor_name":"Mucin-4","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D055271","descriptor_name":"Mucin 5AC","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D055271","descriptor_name":"Mucin 5AC","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":4,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:2628312","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2628312","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Vis","raw_type":"Text"},{"id":"pmid:19158956","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19158956","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular vision","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.358.9283","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.358.9283","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/04/0f/Mol_Vis_2009_Jan_16_15_108-119.tar.gz","raw_type":"text"},{"id":"mag:1567006352","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19158956","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2628312","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2628312","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Vis","raw_type":"Text"},"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W1908394","https://openalex.org/W15148084","https://openalex.org/W52288874","https://openalex.org/W110209143","https://openalex.org/W201419276","https://openalex.org/W1481103396","https://openalex.org/W1491567794","https://openalex.org/W1494175564","https://openalex.org/W1514995438","https://openalex.org/W1535399280","https://openalex.org/W1553374419","https://openalex.org/W1579341601","https://openalex.org/W1608837873","https://openalex.org/W1809656108","https://openalex.org/W1826845578","https://openalex.org/W1854022487","https://openalex.org/W1976009198","https://openalex.org/W1993067940","https://openalex.org/W1997810399","https://openalex.org/W2007976366","https://openalex.org/W2010167207","https://openalex.org/W2017212450","https://openalex.org/W2020695148","https://openalex.org/W2027233329","https://openalex.org/W2027982069","https://openalex.org/W2031533923","https://openalex.org/W2034046486","https://openalex.org/W2037412915","https://openalex.org/W2040714148","https://openalex.org/W2052525810","https://openalex.org/W2057102740","https://openalex.org/W2061348306","https://openalex.org/W2080875750","https://openalex.org/W2089084934","https://openalex.org/W2094488436","https://openalex.org/W2102846605","https://openalex.org/W2104791048","https://openalex.org/W2125369556","https://openalex.org/W2132932170","https://openalex.org/W2137960280","https://openalex.org/W2137965864","https://openalex.org/W2167070447","https://openalex.org/W2250076942","https://openalex.org/W2317666301","https://openalex.org/W2417032266"],"related_works":["https://openalex.org/W7096411877","https://openalex.org/W1674378750","https://openalex.org/W2600357970","https://openalex.org/W1966993983","https://openalex.org/W2020563986","https://openalex.org/W2902869376","https://openalex.org/W2901753686","https://openalex.org/W4233608982","https://openalex.org/W7021171868","https://openalex.org/W861953143"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1],"investigate":[2],"the":[3,32,39,78,94,107,112,119,140,143,157],"compensation":[4,153],"of":[5,34,41,77,91,96,114,118,121,142,159],"secretory":[6,144],"mucins":[7,10,35],"with":[8,13],"membranous":[9,108,134],"in":[11,22,54,101,116,124,147,165,178],"mice":[12],"goblet":[14,25,98,148,167,179],"cell":[15,149,180],"deficiency.":[16,181],"METHODS:":[17],"A":[18,44],"transgenic":[19],"mouse":[20,103],"model":[21],"which":[23],"conjunctival":[24,97],"cells":[26,99],"were":[27,67],"targeted":[28],"was":[29,36,81,87],"generated,":[30],"and":[31,59,83,93,129,170],"expression":[33],"evaluated":[37],"through":[38],"toxicity":[40],"diphtheria":[42],"toxin":[43],"driven":[45],"by":[46],"a":[47],"human":[48],"mucin,":[49,109,135,145],"MUC5AC,":[50,146],"promoter.":[51],"Immunohistochemical":[52],"staining,":[53,123],"situ":[55,125],"hybridization,":[56,126],"electronic":[57,127],"microscopy,":[58,128],"quantitative":[60,130],"reverse":[61],"transcription":[62],"polymerase":[63],"chain":[64],"reaction":[65],"(RT-PCR)":[66],"used":[68],"to":[69],"characterize":[70],"their":[71],"phenotypes.":[72],"RESULTS:":[73],"The":[74,89,133],"external":[75],"appearance":[76],"ocular":[79],"surface":[80],"normal,":[82],"no":[84],"corneal":[85],"pathology":[86],"found.":[88],"quantity":[90],"MUC5AC":[92,115],"number":[95],"decreased":[100],"this":[102],"as":[104],"expected.":[105],"However,":[106],"MUC4,":[110,136],"compensates":[111],"decrease":[113],"terms":[117],"results":[120],"immunohistochemical":[122],"RT-PCR.":[131],"CONCLUSIONS:":[132],"can":[137,162],"compensate":[138],"for":[139],"deficiency":[141,168],"deficient":[150],"mice.":[151],"This":[152],"may":[154,172],"explain":[155],"why":[156],"symptoms":[158],"mucus":[160],"threads":[161],"be":[163],"found":[164],"some":[166],"diseases,":[169],"it":[171],"provide":[173],"an":[174],"alternative":[175],"defensive":[176],"mechanism":[177]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}