{"id":"https://openalex.org/W1564091906","doi":"https://doi.org/10.1186/1471-2334-6-87","title":"A sensitive one-step real-time PCR for detection of avian influenza viruses using a MGB probe and an internal positive control","display_name":"A sensitive one-step real-time PCR for detection of avian influenza viruses using a MGB probe and an internal positive control","publication_year":2006,"publication_date":"2006-05-25","ids":{"openalex":"https://openalex.org/W1564091906","doi":"https://doi.org/10.1186/1471-2334-6-87","mag":"1564091906","pmid":"https://pubmed.ncbi.nlm.nih.gov/16725022","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1524785"},"language":"en","primary_location":{"id":"doi:10.1186/1471-2334-6-87","doi":"https://doi.org/10.1186/1471-2334-6-87","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2334-6-87","pdf_url":"https://bmcinfectdis.biomedcentral.com/counter/pdf/10.1186/1471-2334-6-87","source":{"id":"https://openalex.org/S171015910","display_name":"BMC Infectious Diseases","issn_l":"1471-2334","issn":["1471-2334"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Infectious Diseases","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcinfectdis.biomedcentral.com/counter/pdf/10.1186/1471-2334-6-87","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023870159","display_name":"Livia Di Trani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27897274","display_name":"Istituto Superiore di Sanit\u00e0","ror":"https://ror.org/02hssy432","country_code":"IT","type":"government","lineage":["https://openalex.org/I27897274"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Livia Di Trani","raw_affiliation_strings":["Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161 Rome, Italy. ditrani@iss.it","Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","V.le Regina Elena 299, 00161, Rome, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161 Rome, Italy. ditrani@iss.it","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090541931","display_name":"Barbara Bedini","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27897274","display_name":"Istituto Superiore di Sanit\u00e0","ror":"https://ror.org/02hssy432","country_code":"IT","type":"government","lineage":["https://openalex.org/I27897274"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Barbara Bedini","raw_affiliation_strings":["Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","V.le Regina Elena 299, 00161, Rome, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029848463","display_name":"Isabella Donatelli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27897274","display_name":"Istituto Superiore di Sanit\u00e0","ror":"https://ror.org/02hssy432","country_code":"IT","type":"government","lineage":["https://openalex.org/I27897274"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Isabella Donatelli","raw_affiliation_strings":["Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0","V.le Regina Elena 299, 00161, Rome, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033907752","display_name":"Laura Campitelli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27897274","display_name":"Istituto Superiore di Sanit\u00e0","ror":"https://ror.org/02hssy432","country_code":"IT","type":"government","lineage":["https://openalex.org/I27897274"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Laura Campitelli","raw_affiliation_strings":["Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0","V.le Regina Elena 299, 00161, Rome, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"Department of Parasitic, Immune-Mediated and Infectious Diseases, Istituto Superiore di Sanit\u00e0","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049695057","display_name":"Barbara Chiappini","orcid":"https://orcid.org/0000-0002-5260-5285","observed_orcids":["https://orcid.org/0000-0002-5260-5285"]},"institutions":[{"id":"https://openalex.org/I27897274","display_name":"Istituto Superiore di Sanit\u00e0","ror":"https://ror.org/02hssy432","country_code":"IT","type":"government","lineage":["https://openalex.org/I27897274"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Barbara Chiappini","raw_affiliation_strings":["Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","V.le Regina Elena 299, 00161, Rome, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049480403","display_name":"Maria Alessandra De Marco","orcid":"https://orcid.org/0000-0002-4951-4980","observed_orcids":["https://orcid.org/0000-0002-4951-4980"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Maria Alessandra De Marco","raw_affiliation_strings":["National Wildlife Institute, Italy; Via Ca' Fornacetta 9, 40064, Ozzano Emilia, (BO), Italy","National Wildlife Institute, Italy","Via Ca' Fornacetta 9, 40064, Ozzano Emilia, (BO), Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Wildlife Institute, Italy; Via Ca' Fornacetta 9, 40064, Ozzano Emilia, (BO), Italy","institution_ids":[]},{"raw_affiliation_string":"National Wildlife Institute, Italy","institution_ids":[]},{"raw_affiliation_string":"Via Ca' Fornacetta 9, 40064, Ozzano Emilia, (BO), Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085061966","display_name":"Mauro Delogu","orcid":"https://orcid.org/0000-0003-1279-9094","observed_orcids":["https://orcid.org/0000-0003-1279-9094"]},"institutions":[{"id":"https://openalex.org/I9360294","display_name":"University of Bologna","ror":"https://ror.org/01111rn36","country_code":"IT","type":"education","lineage":["https://openalex.org/I9360294"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Mauro Delogu","raw_affiliation_strings":["Department of Veterinary Public Health and Animal Pathology, University of Bologna,; Via Tolara di Sopra 50, 40064, Ozzano Emilia, (BO), Italy","Department of Veterinary Public Health and Animal Pathology, University of Bologna,","Via Tolara di Sopra 50, 40064, Ozzano Emilia, (BO), Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Public Health and Animal Pathology, University of Bologna,; Via Tolara di Sopra 50, 40064, Ozzano Emilia, (BO), Italy","institution_ids":["https://openalex.org/I9360294"]},{"raw_affiliation_string":"Department of Veterinary Public Health and Animal Pathology, University of Bologna,","institution_ids":["https://openalex.org/I9360294"]},{"raw_affiliation_string":"Via Tolara di Sopra 50, 40064, Ozzano Emilia, (BO), Italy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070097811","display_name":"Canio Buonavoglia","orcid":"https://orcid.org/0000-0002-6149-0678","observed_orcids":["https://orcid.org/0000-0002-6149-0678"]},"institutions":[{"id":"https://openalex.org/I5561750","display_name":"University of Bari Aldo Moro","ror":"https://ror.org/027ynra39","country_code":"IT","type":"education","lineage":["https://openalex.org/I5561750"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Canio Buonavoglia","raw_affiliation_strings":["Department of Animal Health and Well-Being, Faculty of Veterinary Medicine of Bari, Prov. Per Casamassima Valenzano, 70010, Bari, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Health and Well-Being, Faculty of Veterinary Medicine of Bari, Prov. Per Casamassima Valenzano, 70010, Bari, Italy","institution_ids":["https://openalex.org/I5561750"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009250245","display_name":"Gabriele Vaccari","orcid":"https://orcid.org/0000-0003-0215-3521","observed_orcids":["https://orcid.org/0000-0003-0215-3521"]},"institutions":[{"id":"https://openalex.org/I27897274","display_name":"Istituto Superiore di Sanit\u00e0","ror":"https://ror.org/02hssy432","country_code":"IT","type":"government","lineage":["https://openalex.org/I27897274"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Gabriele Vaccari","raw_affiliation_strings":["Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","V.le Regina Elena 299, 00161, Rome, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0; V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0","institution_ids":["https://openalex.org/I27897274"]},{"raw_affiliation_string":"V.le Regina Elena 299, 00161, Rome, Italy","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5023870159"],"corresponding_institution_ids":["https://openalex.org/I27897274"],"apc_list":{"value":3090,"currency":"USD","value_usd":3090},"apc_paid":{"value":3090,"currency":"USD","value_usd":3090},"fwci":5.7204,"has_fulltext":true,"cited_by_count":87,"citation_normalized_percentile":{"value":0.96265378,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"6","issue":"1","first_page":"87","last_page":"87"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/avian-influenza-virus","display_name":"avian influenza virus","score":0.9380000233650208},{"id":"https://openalex.org/keywords/one-step-rt-pcr","display_name":"one-step RT-PCR","score":0.7850000262260437},{"id":"https://openalex.org/keywords/mgb-probe","display_name":"MGB probe","score":0.9679999947547913},{"id":"https://openalex.org/keywords/internal-positive-control","display_name":"internal positive control","score":0.9210000038146973},{"id":"https://openalex.org/keywords/matrix-protein-gene","display_name":"matrix gene","score":0.9120000004768372},{"id":"https://openalex.org/keywords/taqman","display_name":"TaqMan","score":0.6079999804496765},{"id":"https://openalex.org/keywords/influenza-a-subtypes","display_name":"influenza A subtypes","score":0.6370000243186951},{"id":"https://openalex.org/keywords/viral-load","display_name":"viral load","score":0.6010000109672546},{"id":"https://openalex.org/keywords/assay-sensitivity","display_name":"assay sensitivity","score":0.574999988079071},{"id":"https://openalex.org/keywords/false-negative-detection","display_name":"false negative detection","score":0.6779999732971191}],"concepts":[{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7843672633171082},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7375550866127014},{"id":"https://openalex.org/C519248777","wikidata":"https://www.wikidata.org/wiki/Q160122","display_name":"Influenza A virus subtype H5N1","level":3,"score":0.719617486000061},{"id":"https://openalex.org/C143660080","wikidata":"https://www.wikidata.org/wiki/Q7684883","display_name":"TaqMan","level":4,"score":0.7084290981292725},{"id":"https://openalex.org/C184112429","wikidata":"https://www.wikidata.org/wiki/Q476174","display_name":"Serial dilution","level":3,"score":0.5821143388748169},{"id":"https://openalex.org/C190139176","wikidata":"https://www.wikidata.org/wiki/Q598976","display_name":"Medical microbiology","level":2,"score":0.548755407333374},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.526530385017395},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.5050099492073059},{"id":"https://openalex.org/C4692481","wikidata":"https://www.wikidata.org/wiki/Q169526","display_name":"Reverse transcription polymerase chain reaction","level":4,"score":0.48126503825187683},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.46674826741218567},{"id":"https://openalex.org/C2993559633","wikidata":"https://www.wikidata.org/wiki/Q43987","display_name":"Avian influenza virus","level":4,"score":0.4653286337852478},{"id":"https://openalex.org/C2777546802","wikidata":"https://www.wikidata.org/wiki/Q834390","display_name":"Influenza A virus","level":3,"score":0.4601019620895386},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.4182318449020386},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.316864937543869},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12369757890701294},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.11892703175544739},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.08894431591033936},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001715","descriptor_name":"Bird Diseases","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":true},{"descriptor_ui":"D001717","descriptor_name":"Birds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005585","descriptor_name":"Influenza in Birds","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D009980","descriptor_name":"Influenza A virus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":6,"locations":[{"id":"doi:10.1186/1471-2334-6-87","doi":"https://doi.org/10.1186/1471-2334-6-87","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2334-6-87","pdf_url":"https://bmcinfectdis.biomedcentral.com/counter/pdf/10.1186/1471-2334-6-87","source":{"id":"https://openalex.org/S171015910","display_name":"BMC Infectious Diseases","issn_l":"1471-2334","issn":["1471-2334"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Infectious Diseases","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1524785","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1524785","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Infect Dis","raw_type":"Text"},{"id":"pmid:16725022","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16725022","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC infectious diseases","raw_type":"Journal Article"},{"id":"pmh:oai:cris.unibo.it:11585/43600","doi":null,"is_oa":true,"landing_page_url":"https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-6-87","pdf_url":null,"source":{"id":"https://openalex.org/S4306402579","display_name":"Archivio istituzionale della ricerca (Alma Mater Studiorum Universit\u00e0 di Bologna)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210117483","host_organization_name":"Istituto di Ematologia di Bologna","host_organization_lineage":["https://openalex.org/I4210117483"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:3cb40015b351424e8e4ab99b3f0b443a","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/3cb40015b351424e8e4ab99b3f0b443a","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Infectious Diseases, Vol 6, Iss 1, p 87 (2006)","raw_type":"article"},{"id":"pmh:oai:ricerca.uniba.it:11586/105950","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/11586/105950","pdf_url":null,"source":{"id":"https://openalex.org/S4377196296","display_name":"CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I5561750","host_organization_name":"University of Bari Aldo Moro","host_organization_lineage":["https://openalex.org/I5561750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1186/1471-2334-6-87","doi":"https://doi.org/10.1186/1471-2334-6-87","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2334-6-87","pdf_url":"https://bmcinfectdis.biomedcentral.com/counter/pdf/10.1186/1471-2334-6-87","source":{"id":"https://openalex.org/S171015910","display_name":"BMC Infectious Diseases","issn_l":"1471-2334","issn":["1471-2334"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Infectious Diseases","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4043000042438507,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321781","display_name":"Ministero della Salute","ror":"https://ror.org/00789fa95"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1564091906.pdf","grobid_xml":"https://content.openalex.org/works/W1564091906.grobid-xml"},"referenced_works_count":27,"referenced_works":["https://openalex.org/W1893884736","https://openalex.org/W1969584869","https://openalex.org/W1972516281","https://openalex.org/W1974800893","https://openalex.org/W1975424493","https://openalex.org/W2002374989","https://openalex.org/W2018534682","https://openalex.org/W2020771854","https://openalex.org/W2042968066","https://openalex.org/W2079180367","https://openalex.org/W2088861262","https://openalex.org/W2104730345","https://openalex.org/W2113827628","https://openalex.org/W2113884542","https://openalex.org/W2140521217","https://openalex.org/W2140535402","https://openalex.org/W2147456190","https://openalex.org/W2151601375","https://openalex.org/W2155467153","https://openalex.org/W2160881014","https://openalex.org/W2165157887","https://openalex.org/W2168020376","https://openalex.org/W2170081920","https://openalex.org/W2179009119","https://openalex.org/W2214091827","https://openalex.org/W2256756668","https://openalex.org/W3217730596"],"related_works":["https://openalex.org/W2621249636","https://openalex.org/W3010102127","https://openalex.org/W2002344455","https://openalex.org/W2096357035","https://openalex.org/W4323283740","https://openalex.org/W2263727695","https://openalex.org/W4404376130","https://openalex.org/W3130281702","https://openalex.org/W2113884542","https://openalex.org/W2128932527"],"abstract_inverted_index":{"BACKGROUND:":[0],"Avian":[1],"influenza":[2,19,169,180,247],"viruses":[3],"(AIVs)":[4],"are":[5],"endemic":[6],"in":[7,21,47,59,81,190,210,225,338],"wild":[8],"birds":[9],"and":[10,13,153,166,194,262,268,307,331],"their":[11],"introduction":[12],"conversion":[14],"to":[15,39,43,95,146,185,228,243,258,278,318],"highly":[16],"pathogenic":[17],"avian":[18],"virus":[20,170,182],"domestic":[22],"poultry":[23],"is":[24,50,241,294],"a":[25,34,104,113,143,219,273],"cause":[26],"of":[27,56,63,76,86,122,125,174,200,209,218,232,253,291,309,316,335],"serious":[28],"economic":[29],"losses":[30],"as":[31,33,67,222,283,285],"well":[32,284],"risk":[35],"for":[36,52,97,119,320,329,332],"potential":[37],"transmission":[38],"humans.":[40],"The":[41,61,172,197,216,238,250,288,302],"ability":[42],"rapidly":[44],"recognise":[45],"AIVs":[46],"biological":[48],"specimens":[49],"critical":[51],"limiting":[53],"further":[54],"spread":[55],"the":[57,120,159,201,230,233,254,269,292,310,314,333],"disease":[58],"poultry.":[60],"advent":[62],"molecular":[64],"methods":[65,78,88],"such":[66],"real":[68,108],"time":[69,109],"polymerase":[70],"chain":[71],"reaction":[72,267],"has":[73],"allowed":[74],"improvement":[75],"detection":[77,121,251],"currently":[79],"used":[80],"laboratories,":[82],"although":[83],"not":[84,227],"all":[85,245],"these":[87],"include":[89],"an":[90,131,138,223],"Internal":[91],"Positive":[92],"Control":[93],"(IPC)":[94],"monitor":[96,319],"false":[98,321],"negative":[99,322],"results.":[100],"Therefore":[101],"we":[102],"developed":[103,136],"one-step":[105],"reverse":[106],"transcription":[107],"PCR":[110],"(RRT-PCR)":[111],"with":[112,142,313],"Minor":[114],"Groove":[115],"Binder":[116],"(MGB)":[117],"probe":[118,145,154],"different":[123],"subtypes":[124,186],"AIVs.":[126],"This":[127],"technique":[128],"also":[129],"includes":[130],"IPC.":[132],"METHODS:":[133],"RRT-PCR":[134,175,202,239,312],"was":[135,176,204,235,256],"using":[137,206],"improved":[139],"TaqMan":[140],"technology":[141],"MGB":[144],"detect":[147,244],"AI":[148],"from":[149,163,187,276],"reference":[150],"viruses.":[151,249],"Primers":[152],"were":[155],"designed":[156],"based":[157],"on":[158],"matrix":[160,213],"gene":[161,214],"sequences":[162],"most":[164],"animal":[165],"human":[167],"A":[168,181,248],"subtypes.":[171],"specificity":[173,308],"assessed":[177],"by":[178],"detecting":[179],"isolates":[183],"belonging":[184],"H1-H13":[188],"isolated":[189],"avian,":[191],"human,":[192],"swine":[193],"equine":[195],"hosts.":[196],"analytical":[198,289],"sensitivity":[199,290],"assay":[203,234,240,255,293],"determined":[205],"serial":[207],"dilutions":[208],"vitro":[211],"transcribed":[212],"RNA.":[215],"use":[217,315],"rodent":[220],"RNA":[221,264],"IPC":[224,317],"order":[226],"reduce":[229],"efficiency":[231],"adopted.":[236],"RESULTS:":[237],"capable":[242],"tested":[246],"limit":[252],"shown":[257],"be":[259],"between":[260],"5":[261,277,279],"50":[263],"copies":[265,282],"per":[266],"standard":[270],"curve":[271],"demonstrated":[272],"linear":[274],"range":[275],"x":[280],"108":[281],"excellent":[286],"reproducibility.":[287],"10-100":[295],"times":[296],"higher":[297],"than":[298],"conventional":[299],"RT-PCR.":[300],"CONCLUSION:":[301],"high":[303],"sensitivity,":[304],"rapidity,":[305],"reproducibility":[306],"AIV":[311],"results":[323],"can":[324],"make":[325],"this":[326],"method":[327],"suitable":[328],"diagnosis":[330],"evaluation":[334],"viral":[336],"load":[337],"field":[339],"specimens.":[340]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}