{"id":"https://openalex.org/W1563405220","doi":"https://doi.org/10.1016/s0021-9258(19)42079-6","title":"Synthesis and Characterization of the Duplex Block Polymer d(C15A15)\u00b7d(T15G15)","display_name":"Synthesis and Characterization of the Duplex Block Polymer d(C15A15)\u00b7d(T15G15)","publication_year":1974,"publication_date":"1974-11-01","ids":{"openalex":"https://openalex.org/W1563405220","doi":"https://doi.org/10.1016/s0021-9258(19)42079-6","mag":"1563405220","pmid":"https://pubmed.ncbi.nlm.nih.gov/4474171"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)42079-6","doi":"https://doi.org/10.1016/s0021-9258(19)42079-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)42079-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)42079-6","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040121120","display_name":"John F. Burd","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John F. Burd","raw_affiliation_strings":["From the Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5040500435","display_name":"Robert D. Wells","orcid":"https://orcid.org/0000-0002-7240-8473","observed_orcids":["https://orcid.org/0000-0002-7240-8473"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert D. Wells","raw_affiliation_strings":["From the Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706","institution_ids":["https://openalex.org/I135310074"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I135310074"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.9473,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.90763827,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"249","issue":"22","first_page":"7094","last_page":"7101"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10405","display_name":"Advanced Polymer Synthesis and Characterization","score":0.007699999958276749,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oligonucleotide-synthesis","display_name":"oligonucleotide synthesis","score":0.7179999947547913},{"id":"https://openalex.org/keywords/enzymatic-synthesis","display_name":"enzymatic synthesis","score":0.6970000267028809},{"id":"https://openalex.org/keywords/terminal-transferase","display_name":"terminal transferase","score":0.8640000224113464},{"id":"https://openalex.org/keywords/t4-dna-polymerase","display_name":"T4 DNA polymerase","score":0.9959999918937683},{"id":"https://openalex.org/keywords/dnazyme","display_name":"DNAzyme","score":0.32100000977516174},{"id":"https://openalex.org/keywords/circular-dichroism","display_name":"circular dichroism","score":0.8510000109672546},{"id":"https://openalex.org/keywords/uv-absorption","display_name":"UV absorption","score":0.8690000176429749},{"id":"https://openalex.org/keywords/sedimentation","display_name":"sedimentation","score":0.7699999809265137}],"concepts":[{"id":"https://openalex.org/C99611785","wikidata":"https://www.wikidata.org/wiki/Q6453233","display_name":"Duplex (building)","level":3,"score":0.5939894914627075},{"id":"https://openalex.org/C2777210771","wikidata":"https://www.wikidata.org/wiki/Q4927124","display_name":"Block (permutation group theory)","level":2,"score":0.4724327027797699},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.4677306115627289},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.4616132974624634},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42936933040618896},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.31853732466697693},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.24694600701332092},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18417313694953918},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.14161840081214905},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12316262722015381},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.1092022955417633},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.07888209819793701}],"mesh":[{"descriptor_ui":"D000227","descriptor_name":"Adenine Nucleotides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002250","descriptor_name":"Carbon Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D003597","descriptor_name":"Cytosine Nucleotides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003854","descriptor_name":"Deoxyribonucleotides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004254","descriptor_name":"DNA Nucleotidyltransferases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004267","descriptor_name":"DNA Viruses","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006150","descriptor_name":"Guanine Nucleotides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009713","descriptor_name":"Nucleotidyltransferases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D010179","descriptor_name":"Pancreas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010761","descriptor_name":"Phosphorus Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011119","descriptor_name":"Polynucleotides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D013056","descriptor_name":"Spectrophotometry, Ultraviolet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013942","descriptor_name":"Thymine Nucleotides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013950","descriptor_name":"Thymus Gland","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014461","descriptor_name":"Ultracentrifugation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)42079-6","doi":"https://doi.org/10.1016/s0021-9258(19)42079-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)42079-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:4474171","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4474171","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)42079-6","doi":"https://doi.org/10.1016/s0021-9258(19)42079-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)42079-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W154742911","https://openalex.org/W980031318","https://openalex.org/W1484752212","https://openalex.org/W1507957597","https://openalex.org/W1528996249","https://openalex.org/W1542226198","https://openalex.org/W1547755849","https://openalex.org/W1565208875","https://openalex.org/W1569161849","https://openalex.org/W1572454647","https://openalex.org/W1602794716","https://openalex.org/W1730929942","https://openalex.org/W1970999241","https://openalex.org/W1975901655","https://openalex.org/W2002413317","https://openalex.org/W2005389848","https://openalex.org/W2017236313","https://openalex.org/W2055590435","https://openalex.org/W2063198775","https://openalex.org/W2073787422","https://openalex.org/W2093902683","https://openalex.org/W2096595259","https://openalex.org/W2156163511","https://openalex.org/W2159891999","https://openalex.org/W4299736525"],"related_works":["https://openalex.org/W1568256521","https://openalex.org/W2075463216","https://openalex.org/W2055387318","https://openalex.org/W1845847255","https://openalex.org/W2078639574","https://openalex.org/W2019303915","https://openalex.org/W769622830","https://openalex.org/W2108735656","https://openalex.org/W2124452695","https://openalex.org/W7095849189"],"abstract_inverted_index":{"Abstract":[0],"The":[1,12,57,86,109],"synthesis":[2,13],"of":[3,19,81,92,105],"the":[4,49,55,64,70,93,100],"duplex":[5],"block":[6,94,110],"polymer":[7,95,111],"d(C15A15)\u00b7":[8],"d(T15G15)":[9,71],"is":[10],"described.":[11],"was":[14,59,113],"achieved":[15],"by":[16,115],"a":[17],"series":[18],"enzymatic":[20],"steps":[21],"utilizing":[22],"pancreatic":[23],"DNase,":[24],"calf":[25],"thymus":[26],"terminal":[27],"transferase":[28],"and":[29,34,67,84,89,102,107,119],"T4":[30],"DNA":[31],"polymerase.":[32],"Analytical":[33],"preparative":[35,120],"gel":[36,76],"electrophoresis":[37],"techniques,":[38],"which":[39],"resolved":[40],"individual":[41],"oligonucleotides":[42],"according":[43],"to":[44,48],"chain":[45],"length":[46],"up":[47],"50":[50],"mer,":[51],"were":[52,96],"indispensable":[53],"for":[54],"synthesis.":[56],"molecule":[58],"synthesized":[60],"with":[61,75,99],"[14C]dAMP":[62],"in":[63,69,97,117],"d(C15A15)":[65],"strand":[66],"[3H]dGMP":[68],"strand.":[72],"In":[73],"conjunction":[74],"electrophoresis,":[77],"this":[78],"permitted":[79],"evaluation":[80],"product":[82],"size":[83],"homogeneity.":[85],"ultraviolet":[87],"absorbance":[88],"circular":[90],"dichroism":[91],"agreement":[98],"averaged":[101],"additive":[103],"spectra":[104],"d(A)n\u00b7d(T)n":[106],"d(G)n\u00b7d(C)n.":[108],"also":[112],"characterized":[114],"sedimentation":[116],"analytical":[118],"centrifugation":[121],"studies.":[122]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}