{"id":"https://openalex.org/W155877382","doi":null,"title":"Development and characterization of an H2O2-resistant immortal lens epithelial cell line.","display_name":"Development and characterization of an H2O2-resistant immortal lens epithelial cell line.","publication_year":2000,"publication_date":"2000-03-01","ids":{"openalex":"https://openalex.org/W155877382","mag":"155877382","pmid":"https://pubmed.ncbi.nlm.nih.gov/10711701"},"language":"en","primary_location":{"id":"pmid:10711701","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10711701","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112028763","display_name":"Abraham Spector","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2799503643","display_name":"Columbia University Irving Medical Center","ror":"https://ror.org/01esghr10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799503643"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A Spector","raw_affiliation_strings":["Department of Ophthalmology, Columbia University, New York, New York 10032, USA. as42@columbia.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ophthalmology, Columbia University, New York, New York 10032, USA. as42@columbia.edu","institution_ids":["https://openalex.org/I2799503643","https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017085055","display_name":"Ren-Rong Wang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R R Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048540174","display_name":"Wenji Ma","orcid":"https://orcid.org/0000-0003-2840-3015","observed_orcids":["https://orcid.org/0000-0003-2840-3015"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W Ma","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5011270057","display_name":"Norman J. Kleiman","orcid":"https://orcid.org/0000-0002-4725-998X","observed_orcids":["https://orcid.org/0000-0002-4725-998X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"N J Kleiman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.4731,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.80142737,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"41","issue":"3","first_page":"832","last_page":"43"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.9380000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.9380000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10445","display_name":"Antioxidant Activity and Oxidative Stress","score":0.016899999231100082,"subfield":{"id":"https://openalex.org/subfields/2704","display_name":"Biochemistry (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11480","display_name":"Connexins and lens biology","score":0.010200000368058681,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydrogen-peroxide-tolerance","display_name":"hydrogen peroxide resistance","score":0.7459999918937683},{"id":"https://openalex.org/keywords/lens-epithelial-cells","display_name":"lens epithelial cells","score":0.9919999837875366},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.6800000071525574},{"id":"https://openalex.org/keywords/catalase","display_name":"catalase","score":0.7799999713897705},{"id":"https://openalex.org/keywords/glutathione-peroxidase","display_name":"glutathione peroxidase","score":0.996999979019165},{"id":"https://openalex.org/keywords/glutathione-s-transferase","display_name":"glutathione S-transferase","score":0.9169999957084656},{"id":"https://openalex.org/keywords/antioxidant-enzymes","display_name":"antioxidant enzymes","score":0.6349999904632568},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.6830000281333923},{"id":"https://openalex.org/keywords/fenton-reaction","display_name":"Fenton reaction","score":0.9240000247955322},{"id":"https://openalex.org/keywords/metal-chelation","display_name":"metal chelation","score":0.8379999995231628},{"id":"https://openalex.org/keywords/cell-immortalization","display_name":"cell immortalization","score":0.5350000262260437},{"id":"https://openalex.org/keywords/oxidative-stress-adaptation","display_name":"oxidative stress adaptation","score":0.3959999978542328}],"concepts":[{"id":"https://openalex.org/C2778979269","wikidata":"https://www.wikidata.org/wiki/Q106951292","display_name":"Catalase","level":3,"score":0.6727939248085022},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6053026914596558},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5766353607177734},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.5682466626167297},{"id":"https://openalex.org/C165069038","wikidata":"https://www.wikidata.org/wiki/Q420930","display_name":"Glutathione reductase","level":5,"score":0.5506512522697449},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5192320942878723},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5162496566772461},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.5135495662689209},{"id":"https://openalex.org/C2778760513","wikidata":"https://www.wikidata.org/wiki/Q24773045","display_name":"Glutathione peroxidase","level":4,"score":0.44444185495376587},{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.4317018687725067},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4188610315322876},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3675752878189087},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3151136636734009},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001692","descriptor_name":"Biological Transport","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002452","descriptor_name":"Cell Count","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004249","descriptor_name":"DNA Damage","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004351","descriptor_name":"Drug Resistance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D005978","descriptor_name":"Glutathione","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005986","descriptor_name":"Glyceraldehyde 3-Phosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006861","descriptor_name":"Hydrogen Peroxide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D007908","descriptor_name":"Lens, Crystalline","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D007908","descriptor_name":"Lens, Crystalline","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D007908","descriptor_name":"Lens, Crystalline","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018384","descriptor_name":"Oxidative Stress","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:10711701","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10711701","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},{"id":"mag:155877382","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10711701","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W79732551","https://openalex.org/W829757908","https://openalex.org/W967714910","https://openalex.org/W1533824682","https://openalex.org/W1536923222","https://openalex.org/W1564205457","https://openalex.org/W1936380547","https://openalex.org/W1964834425","https://openalex.org/W1967819871","https://openalex.org/W1972070256","https://openalex.org/W1979344810","https://openalex.org/W1981183222","https://openalex.org/W1982870743","https://openalex.org/W1986559765","https://openalex.org/W1993868592","https://openalex.org/W2011520923","https://openalex.org/W2013376841","https://openalex.org/W2032486709","https://openalex.org/W2036955382","https://openalex.org/W2040315386","https://openalex.org/W2050051158","https://openalex.org/W2058481249","https://openalex.org/W2070828755","https://openalex.org/W2072298605","https://openalex.org/W2078953340","https://openalex.org/W2086075185","https://openalex.org/W2093828656","https://openalex.org/W2095805970","https://openalex.org/W2100837269","https://openalex.org/W2119376397","https://openalex.org/W2132607488","https://openalex.org/W2138537430","https://openalex.org/W2162488829","https://openalex.org/W2172209113","https://openalex.org/W2333323134","https://openalex.org/W2406543802","https://openalex.org/W2410000206","https://openalex.org/W2895899326"],"related_works":["https://openalex.org/W1993868592","https://openalex.org/W2013376841","https://openalex.org/W1975629613","https://openalex.org/W1731478028","https://openalex.org/W1979344810","https://openalex.org/W2041959449","https://openalex.org/W2053672706","https://openalex.org/W2027155517","https://openalex.org/W2088391803","https://openalex.org/W2047394516"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1],"determine":[2],"how":[3],"nature":[4,318],"would":[5],"protect":[6],"lens":[7,16],"epithelial":[8,17],"cells":[9,38,50,66,87,93,154,247,282,305],"from":[10,313],"H2O2":[11,26,102,114,160,307,325,336],"stress,":[12,258],"an":[13,111],"immortal":[14],"murine":[15],"cell":[18,61,135,138,221],"line,":[19],"alphaTN4-1,":[20],"was":[21,176,223,260,283,290],"subjected":[22],"to":[23,44,70,225,288,292,321,323,358],"gradually":[24,97],"increasing":[25,98,330],"levels":[27],"over":[28,103],"a":[29,104,214,265,300],"period":[30,105],"of":[31,63,101,106,113,121,168,234,299,333,345,354],"approximately":[32,107,184],"8":[33,108],"months.":[34],"The":[35,73,286,310],"resultant":[36],"conditioned":[37,65,84,153,220,246],"grew":[39],"normally":[40],"when":[41],"exposed":[42],"daily":[43],"125":[45],"microM":[46],"H2O2,":[47],"whereas":[48],"normal":[49,86,164,274,281,304],"died":[51],"within":[52,264],"9":[53],"hours.":[54,117],"This":[55],"communication":[56],"describes":[57],"changes":[58],"in":[59,75,82,204,218,244,251,272,279,342],"the":[60,64,99,152,158,169,219,245,252,273,280,297,324,331,339],"biology":[62],"that":[67,173,239,317],"allowed":[68],"them":[69],"remain":[71],"viable.":[72],"manner":[74],"which":[76],"critical":[77],"biochemical":[78,236],"parameters":[79,123,237,243,269],"were":[80,94,124,270],"affected":[81],"both":[83],"and":[85,130,137,147,179,190,208,230,263,348],"is":[88,316],"also":[89,193,338],"reported.":[90],"METHODS:":[91],"Conditioned":[92],"obtained":[95],"by":[96,327],"concentration":[100],"months,":[109],"introducing":[110],"aliquot":[112],"every":[115],"24":[116],"A":[118],"wide":[119],"spectra":[120],"biological":[122],"evaluated,":[125],"including":[126],"catalase,":[127],"GSH":[128,188,227],"peroxidase":[129,181],"other":[131],"antioxidative":[132,170],"enzyme":[133],"activities,":[134],"number":[136],"viability,":[139],"non-protein":[140],"thiol,":[141],"ATP,":[142],"transport":[143],"systems,":[144],"thymidine":[145],"incorporation,":[146],"DNA":[148,289],"cleavage.":[149],"RESULTS:":[150],"Surprisingly,":[151],"did":[155],"not":[156,284,328],"degrade":[157],"medium":[159],"more":[161],"rapidly":[162],"than":[163],"cells.":[165],"However,":[166],"analyses":[167],"defenses":[171],"indicated":[172,238],"catalase":[174],"activity":[175,332],"increased":[177,195],"60-fold":[178],"glutathione":[180],"(GSH":[182],"Px)":[183],"2.7-fold.":[185],"Glucose-6-phosphate":[186],"dehydrogenase,":[187],"S-transferase,":[189],"GSSG":[191],"reductase":[192],"had":[194],"activity.":[196],"Using":[197],"one-dimensional":[198],"sodium":[199],"dodecyl":[200],"sulfate-polyacrylamide":[201],"gel":[202],"electrophoresis,":[203],"situ":[205],"trypsin":[206],"digestion":[207],"matrix-assisted":[209],"laser":[210],"desorption/ionization":[211],"mass":[212],"spectrometry,":[213],"highly":[215],"amplified":[216],"doublet":[217],"preparation":[222],"shown":[224,291],"be":[226,359],"S-transferase":[228],"alpha-1":[229],"alpha-2":[231],"isomers.":[232],"Examination":[233],"key":[235],"while":[240],"most":[241],"such":[242],"showed":[248],"marked":[249],"decay":[250],"first":[253],"hour":[254],"or":[255],"so":[256],"after":[257],"recovery":[259],"then":[261],"observed":[262],"few":[266],"hours,":[267],"these":[268],"back":[271],"range.":[275],"In":[276,296],"contrast,":[277],"damage":[278,287],"repaired.":[285],"involve":[293],"Fenton":[294],"chemistry.":[295],"presence":[298],"metal":[301],"ion":[302],"chelator,":[303],"survive":[306],"stress.":[308],"CONCLUSIONS:":[309],"overall":[311],"conclusion":[312],"this":[314],"investigation":[315],"has":[319],"chosen":[320],"respond":[322],"stress":[326],"only":[329],"enzymes":[334],"degrading":[335],"but":[337,351],"systems":[340],"involved":[341],"repair,":[343],"generation":[344],"reducing":[346],"potential,":[347],"detoxification.":[349],"All":[350],"one":[352],"system":[353],"those":[355],"evaluated":[356],"appears":[357],"permanently":[360],"modified.":[361]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}