{"id":"https://openalex.org/W1557040318","doi":null,"title":"Activation and role of MAP kinase-dependent pathways in retinal pigment epithelial cells: ERK and RPE cell proliferation.","display_name":"Activation and role of MAP kinase-dependent pathways in retinal pigment epithelial cells: ERK and RPE cell proliferation.","publication_year":2002,"publication_date":"2002-09-01","ids":{"openalex":"https://openalex.org/W1557040318","mag":"1557040318","pmid":"https://pubmed.ncbi.nlm.nih.gov/12202534"},"language":"en","primary_location":{"id":"pmid:12202534","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12202534","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110336999","display_name":"Christiane Hecquet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Christiane Hecquet","raw_affiliation_strings":["Cordeliers Biomedical Institut, National Institute of Health and Medical Research (INSERM) Unit 450, Center for Scientific Research, Paris, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cordeliers Biomedical Institut, National Institute of Health and Medical Research (INSERM) Unit 450, Center for Scientific Research, Paris, France","institution_ids":["https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023373111","display_name":"Gaelle M. Lefevre","orcid":"https://orcid.org/0000-0003-3575-9028","observed_orcids":["https://orcid.org/0000-0003-3575-9028"]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]},{"id":"https://openalex.org/I4210092322","display_name":"Centre de Recherche des Cordeliers","ror":"https://ror.org/00dmms154","country_code":"FR","type":"facility","lineage":["https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I39804081","https://openalex.org/I4210092322"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Ga\u00eblle Lefevre","raw_affiliation_strings":["From the Cordeliers Biomedical Institut, National Institute of Health and Medical Research (INSERM) Unit 450, Center for Scientific Research, Paris, France; and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Cordeliers Biomedical Institut, National Institute of Health and Medical Research (INSERM) Unit 450, Center for Scientific Research, Paris, France; and the","institution_ids":["https://openalex.org/I154526488","https://openalex.org/I4210092322"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058144065","display_name":"Monika Valtink","orcid":"https://orcid.org/0000-0003-3205-1876","observed_orcids":["https://orcid.org/0000-0003-3205-1876"]},"institutions":[{"id":"https://openalex.org/I4210108711","display_name":"University Medical Center Hamburg-Eppendorf","ror":"https://ror.org/01zgy1s35","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I159176309","https://openalex.org/I4210108711"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Monika Valtink","raw_affiliation_strings":["Cornea Bank and Transplantation Laboratory, Department of Ophthalmology, University Clinic of Hambourg-Eppendorf, Hamburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cornea Bank and Transplantation Laboratory, Department of Ophthalmology, University Clinic of Hambourg-Eppendorf, Hamburg, Germany","institution_ids":["https://openalex.org/I4210108711"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027912557","display_name":"Katrin Engelmann","orcid":"https://orcid.org/0000-0001-7115-8161","observed_orcids":["https://orcid.org/0000-0001-7115-8161"]},"institutions":[{"id":"https://openalex.org/I4210108711","display_name":"University Medical Center Hamburg-Eppendorf","ror":"https://ror.org/01zgy1s35","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I159176309","https://openalex.org/I4210108711"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Katrin Engelmann","raw_affiliation_strings":["Cornea Bank and Transplantation Laboratory, Department of Ophthalmology, University Clinic of Hambourg-Eppendorf, Hamburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cornea Bank and Transplantation Laboratory, Department of Ophthalmology, University Clinic of Hambourg-Eppendorf, Hamburg, Germany","institution_ids":["https://openalex.org/I4210108711"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027580558","display_name":"Fr\u00e9d\u00e9ric Mascarelli","orcid":"https://orcid.org/0000-0002-4022-0257","observed_orcids":["https://orcid.org/0000-0002-4022-0257"]},"institutions":[{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]},{"id":"https://openalex.org/I4210092322","display_name":"Centre de Recherche des Cordeliers","ror":"https://ror.org/00dmms154","country_code":"FR","type":"facility","lineage":["https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I39804081","https://openalex.org/I4210092322"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Frederic Mascarelli","raw_affiliation_strings":["From the Cordeliers Biomedical Institut, National Institute of Health and Medical Research (INSERM) Unit 450, Center for Scientific Research, Paris, France; and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Cordeliers Biomedical Institut, National Institute of Health and Medical Research (INSERM) Unit 450, Center for Scientific Research, Paris, France; and the","institution_ids":["https://openalex.org/I154526488","https://openalex.org/I4210092322"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.3551,"has_fulltext":false,"cited_by_count":87,"citation_normalized_percentile":{"value":0.90537319,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"43","issue":"9","first_page":"3091","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.7163000106811523,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.7163000106811523,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.1639000028371811,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.07199999690055847,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/retinal-pigment-epithelium","display_name":"retinal pigment epithelium","score":0.9330000281333923},{"id":"https://openalex.org/keywords/rpe-cell-proliferation","display_name":"RPE cell proliferation","score":0.906000018119812},{"id":"https://openalex.org/keywords/erk2","display_name":"ERK2","score":0.7730000019073486},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.6970000267028809},{"id":"https://openalex.org/keywords/ras-mapk-pathway","display_name":"RAS/MAPK pathway","score":0.9620000123977661},{"id":"https://openalex.org/keywords/mek1-2-inhibition","display_name":"MEK1/2 inhibition","score":0.8180000185966492},{"id":"https://openalex.org/keywords/cyclin-d1","display_name":"cyclin D1","score":0.9190000295639038},{"id":"https://openalex.org/keywords/fetal-bovine-serum","display_name":"fetal bovine serum","score":0.6320000290870667},{"id":"https://openalex.org/keywords/raf-1","display_name":"Raf-1","score":0.6399999856948853},{"id":"https://openalex.org/keywords/p90-ribosomal-s6-kinase","display_name":"p90 ribosomal S6 kinase","score":0.8709999918937683},{"id":"https://openalex.org/keywords/posterior-segment-diseases","display_name":"posterior segment diseases","score":0.39500001072883606}],"concepts":[{"id":"https://openalex.org/C57074206","wikidata":"https://www.wikidata.org/wiki/Q14197552","display_name":"MAPK/ERK pathway","level":3,"score":0.7648744583129883},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6765314340591431},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.6712872982025146},{"id":"https://openalex.org/C2780044377","wikidata":"https://www.wikidata.org/wiki/Q24778284","display_name":"Ribosomal s6 kinase","level":5,"score":0.5166457295417786},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.5162441730499268},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48040130734443665},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.46921220421791077},{"id":"https://openalex.org/C132149769","wikidata":"https://www.wikidata.org/wiki/Q899651","display_name":"Mitogen-activated protein kinase","level":3,"score":0.43136897683143616},{"id":"https://openalex.org/C75217442","wikidata":"https://www.wikidata.org/wiki/Q423650","display_name":"Protein kinase B","level":3,"score":0.23832523822784424},{"id":"https://openalex.org/C104202773","wikidata":"https://www.wikidata.org/wiki/Q18031289","display_name":"P70-S6 Kinase 1","level":4,"score":0.14173081517219543},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10700467228889465}],"mesh":[{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006133","descriptor_name":"Growth Substances","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010857","descriptor_name":"Pigment Epithelium of Eye","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D010857","descriptor_name":"Pigment Epithelium of Eye","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016283","descriptor_name":"Proto-Oncogene Proteins p21(ras)","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D016283","descriptor_name":"Proto-Oncogene Proteins p21(ras)","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019084","descriptor_name":"Fluorescent Antibody Technique, Indirect","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019908","descriptor_name":"Proto-Oncogene Proteins c-raf","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D019908","descriptor_name":"Proto-Oncogene Proteins c-raf","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019938","descriptor_name":"Cyclin D1","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020319","descriptor_name":"Oligodeoxyribonucleotides, Antisense","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D020928","descriptor_name":"Mitogen-Activated Protein Kinases","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D020929","descriptor_name":"Mitogen-Activated Protein Kinase Kinases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D020929","descriptor_name":"Mitogen-Activated Protein Kinase Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020935","descriptor_name":"MAP Kinase Signaling System","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:12202534","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12202534","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},{"id":"mag:1557040318","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12202534","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W47066878","https://openalex.org/W226556843","https://openalex.org/W1485413088","https://openalex.org/W1491629634","https://openalex.org/W1970061904","https://openalex.org/W1974760224","https://openalex.org/W1978686430","https://openalex.org/W1985888026","https://openalex.org/W1988260684","https://openalex.org/W1991222632","https://openalex.org/W1993876642","https://openalex.org/W2007283930","https://openalex.org/W2007988639","https://openalex.org/W2011311545","https://openalex.org/W2019687915","https://openalex.org/W2022635808","https://openalex.org/W2041890623","https://openalex.org/W2045401053","https://openalex.org/W2059986458","https://openalex.org/W2068698007","https://openalex.org/W2070403771","https://openalex.org/W2075104267","https://openalex.org/W2081137133","https://openalex.org/W2082031648","https://openalex.org/W2083796765","https://openalex.org/W2095126097","https://openalex.org/W2127150495","https://openalex.org/W2141164706","https://openalex.org/W2142258828","https://openalex.org/W2143461093","https://openalex.org/W2313391367","https://openalex.org/W2314472510","https://openalex.org/W2398423209","https://openalex.org/W2418674944"],"related_works":["https://openalex.org/W2319545202","https://openalex.org/W2392798132","https://openalex.org/W2074266196","https://openalex.org/W7096886057","https://openalex.org/W1179783128","https://openalex.org/W2070122670","https://openalex.org/W94833967","https://openalex.org/W1494192684","https://openalex.org/W3031123393","https://openalex.org/W2034922411"],"abstract_inverted_index":{"PURPOSE:":[0],"Retinal":[1],"pigment":[2],"epithelial":[3],"(RPE)":[4],"cell":[5,31,54,61,65,117,126,152,176,195,207,222,243,248,274,290],"proliferation":[6,66,127,153,208,249,275],"plays":[7,281],"a":[8,282],"key":[9,283],"role":[10,265,284],"in":[11,27,53,115,143,216,220,268,273,285],"the":[12,18,28,39,42,48,57,77,111,168,186,217,263,278,286,299,306],"pathogenesis":[13],"of":[14,30,41,59,76,147,167,199,203,226,236,266,288,301,308],"ocular":[15],"diseases":[16],"involving":[17],"posterior":[19],"segment.":[20],"Mitogen-activated":[21],"protein":[22,98],"kinases":[23,187],"(MAPKs)":[24],"are":[25],"involved":[26,114,219],"control":[29,287],"proliferation.":[32,62,118,177,196,291,310],"This":[33],"study":[34],"was":[35,67,87,171],"conducted":[36],"to":[37,109,156,161],"investigate":[38],"involvement":[40],"extracellular":[43],"signal-regulated":[44,82],"kinase":[45,80,83,184],"(ERK)":[46,84],"pathway,":[47],"major":[49],"MAPK":[50],"pathway":[51,86,170,280],"implicated":[52],"growth":[55,133,138],"during":[56,194],"induction":[58],"RPE":[60,64,125,175,221,247,289],"METHODS:":[63],"stimulated":[68],"with":[69,95],"10%":[70],"fetal":[71],"calf":[72],"serum":[73],"(FCS).":[74],"Activation":[75],"Ras/Raf/MAP":[78],"kinase-ERK":[79],"(MEK)/extracellular":[81],"signaling":[85,113,218],"detected":[88],"by":[89,154,159,209,239,252],"Western":[90],"blot":[91],"analysis":[92],"and":[93,101,149,158,180,234,262,272],"immunochemistry":[94],"specific":[96],"anti-phosphosignaling":[97],"antibodies.":[99],"Pharmacologic":[100,145,197],"antisense":[102],"(AS)":[103],"oligonucleotide":[104],"(ODN)":[105],"strategies":[106],"were":[107,191],"used":[108],"analyze":[110],"ERK":[112],"serum-induced":[116],"RESULTS:":[119],"FCS":[120],"(10%)":[121],"induced":[122],"more":[123,302],"vigorous":[124],"than":[128],"did":[129],"FGF2,":[130],"VEGF,":[131],"platelet-derived":[132],"factor":[134,139],"(PDGF),":[135],"or":[136,142],"epidermal":[137],"(EGF),":[140],"alone":[141],"combination.":[144],"inhibition":[146,198,225,235,307],"Ras":[148,261],"Raf-1":[150],"reduced":[151,206,230],"67%":[155],"100%":[157],"62%":[160],"79%,":[162],"respectively,":[163],"demonstrating":[164],"that":[165,213,246,277],"activation":[166,202],"Ras/Raf-1":[169],"essential":[172],"for":[173,260,298,305],"FCS-induced":[174],"MEK1/2,":[178],"ERK2,":[179,190,204],"P90":[181],"ribosomal":[182],"S6":[183],"(P90(RSK)),":[185],"downstream":[188],"from":[189],"strongly":[192],"activated":[193],"MEK1/2":[200],"abolished":[201],"but":[205],"only":[210],"32%,":[211],"showing":[212],"MEK/ERK":[214],"participates":[215],"growth.":[223],"Both":[224],"ERK2":[227,267],"activation,":[228],"which":[229],"cyclin":[231,237,253,269],"D1":[232,238,254,270],"production,":[233],"AS":[240],"ODN":[241],"decreased":[242],"proliferation,":[244],"suggesting":[245],"is":[250],"mediated":[251],"through":[255],"ERK2.":[256],"CONCLUSIONS:":[257],"The":[258],"requirement":[259],"regulatory":[264],"production":[271],"suggest":[276],"Ras/Raf/MEK/ERK":[279],"These":[292],"data":[293],"may":[294],"have":[295],"important":[296],"implications":[297],"development":[300],"selective":[303],"methods":[304],"retinal":[309]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":6},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":9},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
