{"id":"https://openalex.org/W1556903685","doi":"https://doi.org/10.1016/s0021-9258(18)90844-6","title":"Effect of supercoiling on the abortive initiation kinetics of the RNA-I promoter of ColE1 plasmid DNA.","display_name":"Effect of supercoiling on the abortive initiation kinetics of the RNA-I promoter of ColE1 plasmid DNA.","publication_year":1984,"publication_date":"1984-09-01","ids":{"openalex":"https://openalex.org/W1556903685","doi":"https://doi.org/10.1016/s0021-9258(18)90844-6","mag":"1556903685","pmid":"https://pubmed.ncbi.nlm.nih.gov/6206065"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)90844-6","doi":"https://doi.org/10.1016/s0021-9258(18)90844-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)90844-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)90844-6","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103512044","display_name":"David Carrico Wood","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D C Wood","raw_affiliation_strings":["From the Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama 35294"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama 35294","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025309149","display_name":"Jacob Lebowitz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Lebowitz","raw_affiliation_strings":["From the Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama 35294"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama 35294","institution_ids":["https://openalex.org/I32389192"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I32389192"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.4955,"has_fulltext":false,"cited_by_count":60,"citation_normalized_percentile":{"value":0.92618958,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"259","issue":"18","first_page":"11184","last_page":"11187"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9824000000953674,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9824000000953674,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.013100000098347664,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-supercoiling","display_name":"DNA supercoiling","score":0.9980000257492065},{"id":"https://openalex.org/keywords/abortive-initiation","display_name":"abortive initiation","score":0.9169999957084656},{"id":"https://openalex.org/keywords/cole1-plasmid","display_name":"ColE1 plasmid","score":0.9879999756813049},{"id":"https://openalex.org/keywords/isomerization-rate-constants","display_name":"isomerization rate constants","score":0.843999981880188},{"id":"https://openalex.org/keywords/rna-polymerase","display_name":"RNA polymerase","score":0.5680000185966492}],"concepts":[{"id":"https://openalex.org/C2777014509","wikidata":"https://www.wikidata.org/wiki/Q5141800","display_name":"ColE1","level":4,"score":0.8866515159606934},{"id":"https://openalex.org/C158456008","wikidata":"https://www.wikidata.org/wiki/Q412639","display_name":"DNA supercoil","level":4,"score":0.852645993232727},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.7432377338409424},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6606165766716003},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6234241127967834},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5893307328224182},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.572084367275238},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4496411383152008},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4199836254119873},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.34861525893211365},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.32408833503723145},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.21555420756340027},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12937775254249573},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004278","descriptor_name":"DNA, Superhelical","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008433","descriptor_name":"Mathematics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009876","descriptor_name":"Operon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)90844-6","doi":"https://doi.org/10.1016/s0021-9258(18)90844-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)90844-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:6206065","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6206065","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)90844-6","doi":"https://doi.org/10.1016/s0021-9258(18)90844-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)90844-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1581531732","https://openalex.org/W1595075385","https://openalex.org/W1970237463","https://openalex.org/W1971298388","https://openalex.org/W1971377031","https://openalex.org/W1974378702","https://openalex.org/W1979700870","https://openalex.org/W1980879384","https://openalex.org/W1980895652","https://openalex.org/W1998831601","https://openalex.org/W2006594688","https://openalex.org/W2025095860","https://openalex.org/W2027784145","https://openalex.org/W2028198542","https://openalex.org/W2029375782","https://openalex.org/W2030551247","https://openalex.org/W2042307633","https://openalex.org/W2049715604","https://openalex.org/W2079251780","https://openalex.org/W2080616338","https://openalex.org/W2082063129","https://openalex.org/W2134624279"],"related_works":["https://openalex.org/W2063836748","https://openalex.org/W2070974192","https://openalex.org/W2737429962","https://openalex.org/W2030336276","https://openalex.org/W2133026380","https://openalex.org/W227611081","https://openalex.org/W2079301973","https://openalex.org/W1986301815","https://openalex.org/W2033430376","https://openalex.org/W2052104258"],"abstract_inverted_index":{"An":[0],"analysis":[1,96],"of":[2,5,10,14,68,97,105],"the":[3,11,20,49,58,78,91,103],"effect":[4,67,86],"DNA":[6],"supercoiling":[7,69],"on":[8,70,87],"transcription":[9,99],"RNA-I":[12,71,98],"promoter":[13],"ColE1":[15],"has":[16],"been":[17],"undertaken":[18],"using":[19],"abortive":[21,94],"initiation":[22,95],"assay":[23],"developed":[24],"by":[25],"McClure":[26],"(McClure,":[27],"W.":[28],"R.,":[29],"Cech,":[30],"C.":[31],"L.,":[32],"and":[33,57],"Johnston,":[34],"D.":[35],"E.":[36],"(1978)":[37],"J.":[38],"Biol.":[39],"Chem.":[40],"253,":[41],"8941-8948).":[42],"A":[43],"tau":[44],"plot":[45],"was":[46],"constructed,":[47],"enabling":[48],"equilibrium":[50],"constant":[51,61],"for":[52],"closed":[53],"complex":[54],"formation":[55],"(KI)":[56],"isomerization":[59,79],"rate":[60],"(k2)":[62],"to":[63],"be":[64],"determined.":[65],"The":[66],"is":[72,90],"an":[73],"apparent":[74],"100-fold":[75],"increase":[76],"in":[77,102],"rate,":[80],"k2,":[81],"with":[82],"little":[83],"or":[84],"no":[85],"KI.":[88],"This":[89],"first":[92],"reported":[93],"which":[100],"serves":[101],"regulation":[104],"plasmid":[106],"replication.":[107]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}