{"id":"https://openalex.org/W1555092004","doi":"https://doi.org/10.1016/s0021-9258(18)47797-6","title":"Immunoaffinity purification and characterization of vacuolar H+ATPase from bovine kidney.","display_name":"Immunoaffinity purification and characterization of vacuolar H+ATPase from bovine kidney.","publication_year":1987,"publication_date":"1987-11-01","ids":{"openalex":"https://openalex.org/W1555092004","doi":"https://doi.org/10.1016/s0021-9258(18)47797-6","mag":"1555092004","pmid":"https://pubmed.ncbi.nlm.nih.gov/2890634"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)47797-6","doi":"https://doi.org/10.1016/s0021-9258(18)47797-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)47797-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W1555092004"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)47797-6","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108791692","display_name":"Stephen L. Gluck","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S Gluck","raw_affiliation_strings":["Department of Medicine, University of Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"last","author":{"id":null,"display_name":"J Caldwell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Caldwell","raw_affiliation_strings":["Department of Medicine, University of Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I40347166"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":5.5762,"has_fulltext":false,"cited_by_count":206,"citation_normalized_percentile":{"value":0.97107425,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"262","issue":"32","first_page":"15780","last_page":"15789"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/proton-translocating-atpase","display_name":"proton-translocating ATPase","score":0.8019999861717224},{"id":"https://openalex.org/keywords/immunoaffinity-purification","display_name":"immunoaffinity purification","score":0.8379999995231628},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.7799999713897705},{"id":"https://openalex.org/keywords/bovine-kidney","display_name":"bovine kidney","score":0.8019999861717224},{"id":"https://openalex.org/keywords/microsomal-membranes","display_name":"microsomal membranes","score":0.7540000081062317},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.6000000238418579},{"id":"https://openalex.org/keywords/divalent-cation-inhibition","display_name":"divalent cation inhibition","score":0.7630000114440918},{"id":"https://openalex.org/keywords/n-ethylmaleimide","display_name":"N-ethylmaleimide","score":0.7459999918937683},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.34299999475479126},{"id":"https://openalex.org/keywords/membrane-reconstitution","display_name":"membrane reconstitution","score":0.4099999964237213},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.35899999737739563}],"concepts":[{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.5686935186386108},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5369967818260193},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39743107557296753},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3407668471336365},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28703540563583374},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.21336451172828674}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006180","descriptor_name":"Proton-Translocating ATPases","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006180","descriptor_name":"Proton-Translocating ATPases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006180","descriptor_name":"Proton-Translocating ATPases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008055","descriptor_name":"Lipids","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014617","descriptor_name":"Vacuoles","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)47797-6","doi":"https://doi.org/10.1016/s0021-9258(18)47797-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)47797-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2890634","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2890634","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)47797-6","doi":"https://doi.org/10.1016/s0021-9258(18)47797-6","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)47797-6","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2879999188","display_name":"CHARACTERIZATION OF H+ATPASE IN URINARY ACIDIFICATION","funder_award_id":"1r01am034788-01","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W39387605","https://openalex.org/W940824992","https://openalex.org/W1497289365","https://openalex.org/W1528710182","https://openalex.org/W1542880421","https://openalex.org/W1543955271","https://openalex.org/W1560944359","https://openalex.org/W1565514826","https://openalex.org/W1566117661","https://openalex.org/W1584402017","https://openalex.org/W1588912020","https://openalex.org/W1594934722","https://openalex.org/W1606423922","https://openalex.org/W1651085429","https://openalex.org/W1674580118","https://openalex.org/W1775749144","https://openalex.org/W1871209444","https://openalex.org/W1984532185","https://openalex.org/W1986204805","https://openalex.org/W1992900472","https://openalex.org/W2005910022","https://openalex.org/W2006207612","https://openalex.org/W2025935390","https://openalex.org/W2040694105","https://openalex.org/W2050687517","https://openalex.org/W2069712386","https://openalex.org/W2087552293","https://openalex.org/W2096750579","https://openalex.org/W2171675357","https://openalex.org/W2174160083"],"related_works":["https://openalex.org/W1531617221","https://openalex.org/W56865634","https://openalex.org/W2416456951","https://openalex.org/W1588912020","https://openalex.org/W1481801182","https://openalex.org/W1508107852","https://openalex.org/W1835116044","https://openalex.org/W1666994550","https://openalex.org/W1516279268","https://openalex.org/W2078451023"],"abstract_inverted_index":{"Vacuolar":[0],"proton-translocating":[1],"ATPase":[2],"from":[3,46,267],"bovine":[4,48],"kidney":[5,49,253],"was":[6,55,68,92,126,145,183,200],"purified":[7,73,81,97,143,165,252],"in":[8,70,246,262],"one":[9],"step":[10],"by":[11,57,74,94,147,203],"immunoprecipitation":[12],"and":[13,44,88,113,115,176,232,264],"immunoaffinity":[14,80,164],"chromatography":[15],"using":[16],"an":[17,75],"immobilized":[18],"anti-H+ATPase":[19],"monoclonal":[20,23],"antibody.":[21],"The":[22,79,96,123,141,163,198,250],"antibody":[24],"affinity":[25,142,251],"matrix":[26],"coprecipitated":[27],"polypeptides":[28,67],"with":[29],"Mr":[30],"of":[31,66,120,214,243],"70,000,":[32],"a":[33,52,116],"cluster":[34],"at":[35,111],"56,000,":[36],"45,000,":[37],"42,000,":[38],"38,000,":[39],"33,000,":[40],"31,000,":[41],"15,000,":[42],"14,000,":[43],"12,000":[45],"solubilized":[47],"microsomal":[50],"membranes,":[51],"pattern":[53,65],"that":[54,91],"unaffected":[56],"different":[58],"detergent":[59],"washing":[60],"conditions.":[61],"A":[62],"nearly":[63],"identical":[64],"observed":[69],"H+ATPase":[71,82,144],"partially":[72],"entirely":[76],"independent":[77],"method.":[78],"had":[83,99,209],"reconstitutively":[84],"active":[85],"ATP-induced":[86],"acidification":[87],"potential":[89],"generation":[90],"inhibited":[93,202],"N-ethylamaleimide.":[95],"enzyme":[98,166,199,254],"specific":[100],"activities":[101],"as":[102,104],"high":[103],"3.1":[105],"mumol/min/mg":[106],"protein,":[107],"dual":[108],"pH":[109],"optima":[110],"6.5":[112],"7.2,":[114],"Km":[117],"for":[118,174,181,217,221,225,229,235,241,270],"ATP":[119,127],"150":[121],"microM.":[122],"substrate":[124],"preference":[125,180],"greater":[128,132,135,138,150,155,159,187,190,194],"than":[129,133,136,139,151,156,160,188,191,195],"ITP":[130],"much":[131,154,186,193],"UTP":[134],"GTP":[137],"CTP.":[140],"stimulated":[146],"phosphatidyl":[148,152,157,161],"glycerol":[149],"inositol":[153],"choline":[158],"serine.":[162],"did":[167],"not":[168,201],"require":[169],"monovalent":[170],"anions":[171],"or":[172,206],"cations":[173],"activity,":[175],"the":[177,247],"divalent":[178],"cation":[179],"activity":[182],"Mn,":[184],"Mg":[185],"Ca":[189],"Co":[192],"Sr,":[196],"Ba.":[197],"ouabain,":[204],"azide,":[205],"vanadate,":[207],"but":[208,258],"kappa":[210],"1/2":[211],"inhibitory":[212],"concentrations":[213],"22.2":[215],"microM":[216,220,224,228,234],"N-ethylmaleimide,":[218],"14.9":[219],"NBD-Cl,":[222],"4.9":[223],"N,N'-dicyclohexylcarbodiimide,":[226],"13.8":[227],"4,4'-diisothiocyanatostilbene-2,2'-disulfonic":[230],"acid,":[231],"315":[233],"Zn,":[236],"values":[237],"close":[238],"to":[239,257],"those":[240,268],"inhibition":[242],"proton":[244],"transport":[245],"native":[248],"vesicles.":[249],"has":[255],"similarities":[256],"also":[259],"significant":[260],"differences":[261],"structural":[263],"enzymatic":[265],"properties":[266],"reported":[269],"other":[271],"vacuolar":[272],"H+ATPase.":[273]},"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}
