{"id":"https://openalex.org/W155473185","doi":null,"title":"Infectious full-length clones of plant viruses and their use for construction of viral vectors.","display_name":"Infectious full-length clones of plant viruses and their use for construction of viral vectors.","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W155473185","mag":"155473185","pmid":"https://pubmed.ncbi.nlm.nih.gov/18197730"},"language":"en","primary_location":{"id":"pmid:18197730","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18197730","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta virologica","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058170673","display_name":"Al\u017ebeta Nagyov\u00e1","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74788687","display_name":"Comenius University Bratislava","ror":"https://ror.org/0587ef340","country_code":"SK","type":"education","lineage":["https://openalex.org/I74788687"]}],"countries":["SK"],"is_corresponding":false,"raw_author_name":"A Nagyov\u00e1","raw_affiliation_strings":["Faculty of Natural Sciences, Commenius University, Bratislava, Slovak Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Natural Sciences, Commenius University, Bratislava, Slovak Republic","institution_ids":["https://openalex.org/I74788687"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076948146","display_name":"Zdeno \u0160ubr","orcid":"https://orcid.org/0000-0002-5812-2081","observed_orcids":["https://orcid.org/0000-0002-5812-2081"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Z Subr","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.8346,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.66179708,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"51","issue":"4","first_page":"223","last_page":"37"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plant-viruses","display_name":"plant viruses","score":0.8880000114440918},{"id":"https://openalex.org/keywords/infectious-clone","display_name":"infectious clone","score":0.6779999732971191},{"id":"https://openalex.org/keywords/viral-vectors","display_name":"viral vectors","score":0.8550000190734863},{"id":"https://openalex.org/keywords/viral-gene-function","display_name":"viral gene function","score":0.41100001335144043},{"id":"https://openalex.org/keywords/virus-host-interactions","display_name":"host-virus interaction","score":0.9950000047683716},{"id":"https://openalex.org/keywords/transient-expression","display_name":"transient expression","score":0.6179999709129333},{"id":"https://openalex.org/keywords/plant-made-pharmaceuticals","display_name":"plant-made pharmaceuticals","score":0.6710000038146973},{"id":"https://openalex.org/keywords/virus-induced-gene-silencing","display_name":"virus-induced gene silencing","score":0.875},{"id":"https://openalex.org/keywords/plant-bioreactors","display_name":"plant bioreactors","score":0.6159999966621399}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7542638778686523},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6165555715560913},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6133227348327637},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5633676052093506},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5567511916160583},{"id":"https://openalex.org/C202878990","wikidata":"https://www.wikidata.org/wiki/Q830465","display_name":"Capsid","level":3,"score":0.547004222869873},{"id":"https://openalex.org/C109110057","wikidata":"https://www.wikidata.org/wiki/Q1495434","display_name":"Plant virus","level":3,"score":0.5078981518745422},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.49533572793006897},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.4816971719264984},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.46285808086395264},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36479127407073975},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.15336862206459045}],"mesh":[{"descriptor_ui":"D005821","descriptor_name":"Genetic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010942","descriptor_name":"Plant Viruses","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:18197730","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18197730","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta virologica","raw_type":"Journal Article"},{"id":"mag:155473185","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18197730","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4043000042438507}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":129,"referenced_works":["https://openalex.org/W18602428","https://openalex.org/W175394506","https://openalex.org/W201050536","https://openalex.org/W572223481","https://openalex.org/W1494525421","https://openalex.org/W1496245560","https://openalex.org/W1519227646","https://openalex.org/W1533450735","https://openalex.org/W1542833613","https://openalex.org/W1571910039","https://openalex.org/W1615303957","https://openalex.org/W1633767287","https://openalex.org/W1828996061","https://openalex.org/W1966884297","https://openalex.org/W1967122685","https://openalex.org/W1968838674","https://openalex.org/W1971288624","https://openalex.org/W1972545077","https://openalex.org/W1973131117","https://openalex.org/W1974103061","https://openalex.org/W1975643266","https://openalex.org/W1975841569","https://openalex.org/W1977096351","https://openalex.org/W1977972142","https://openalex.org/W1978193049","https://openalex.org/W1978505071","https://openalex.org/W1978567860","https://openalex.org/W1979113876","https://openalex.org/W1982676247","https://openalex.org/W1983873588","https://openalex.org/W1984315188","https://openalex.org/W1985374701","https://openalex.org/W1985794521","https://openalex.org/W1993338003","https://openalex.org/W1994150718","https://openalex.org/W1994806396","https://openalex.org/W1996232227","https://openalex.org/W1996381524","https://openalex.org/W1999099212","https://openalex.org/W1999989729","https://openalex.org/W2000779362","https://openalex.org/W2002062036","https://openalex.org/W2002071775","https://openalex.org/W2002841189","https://openalex.org/W2005108546","https://openalex.org/W2005735357","https://openalex.org/W2007053903","https://openalex.org/W2007103968","https://openalex.org/W2007885388","https://openalex.org/W2008012488","https://openalex.org/W2011417416","https://openalex.org/W2013241878","https://openalex.org/W2017099106","https://openalex.org/W2018615744","https://openalex.org/W2019798878","https://openalex.org/W2020104169","https://openalex.org/W2022913513","https://openalex.org/W2024460002","https://openalex.org/W2024493363","https://openalex.org/W2026168006","https://openalex.org/W2027007032","https://openalex.org/W2027110871","https://openalex.org/W2028421032","https://openalex.org/W2028691640","https://openalex.org/W2029281686","https://openalex.org/W2029679619","https://openalex.org/W2029858177","https://openalex.org/W2033116578","https://openalex.org/W2039310257","https://openalex.org/W2044280621","https://openalex.org/W2046385661","https://openalex.org/W2048479499","https://openalex.org/W2049663107","https://openalex.org/W2052586802","https://openalex.org/W2059090752","https://openalex.org/W2059573212","https://openalex.org/W2060801431","https://openalex.org/W2063565797","https://openalex.org/W2066156204","https://openalex.org/W2066763736","https://openalex.org/W2067037399","https://openalex.org/W2068166698","https://openalex.org/W2068242486","https://openalex.org/W2068614107","https://openalex.org/W2068829680","https://openalex.org/W2071558673","https://openalex.org/W2075704510","https://openalex.org/W2077538947","https://openalex.org/W2078119356","https://openalex.org/W2078586926","https://openalex.org/W2079162269","https://openalex.org/W2081155239","https://openalex.org/W2083309547","https://openalex.org/W2085009837","https://openalex.org/W2085601465","https://openalex.org/W2085825896","https://openalex.org/W2086712066","https://openalex.org/W2088419270","https://openalex.org/W2091145782","https://openalex.org/W2092465742","https://openalex.org/W2095741456","https://openalex.org/W2096325741","https://openalex.org/W2098386715","https://openalex.org/W2102317010","https://openalex.org/W2103653268","https://openalex.org/W2109187213","https://openalex.org/W2112250293","https://openalex.org/W2112645363","https://openalex.org/W2115178105","https://openalex.org/W2115405950","https://openalex.org/W2120165772","https://openalex.org/W2122278014","https://openalex.org/W2124129179","https://openalex.org/W2129542363","https://openalex.org/W2133993065","https://openalex.org/W2134361835","https://openalex.org/W2137541144","https://openalex.org/W2144346879","https://openalex.org/W2145453749","https://openalex.org/W2147049946","https://openalex.org/W2148257122","https://openalex.org/W2151860946","https://openalex.org/W2160645529","https://openalex.org/W2164527203","https://openalex.org/W2164865896","https://openalex.org/W2165704464","https://openalex.org/W2169238846","https://openalex.org/W2173167193","https://openalex.org/W2273279731"],"related_works":["https://openalex.org/W3033918756","https://openalex.org/W48324171","https://openalex.org/W6947317889","https://openalex.org/W2341794182","https://openalex.org/W7086626844","https://openalex.org/W126725791","https://openalex.org/W2164527203","https://openalex.org/W2087383080","https://openalex.org/W2325670474","https://openalex.org/W2078586926"],"abstract_inverted_index":{"In":[0],"the":[1,27,64,77,87,125,128,163,172],"last":[2],"two":[3],"decades,":[4],"preparation":[5],"of":[6,12,29,63,68,84,109,115,124,131,157],"infectious":[7],"RNA":[8],"or":[9,54],"DNA":[10],"clones":[11],"plant":[13,110,116],"viruses":[14],"has":[15,90],"transformed":[16,41],"to":[17,44,56,136],"a":[18,45,57,96,137],"standard":[19],"laboratory":[20],"technique,":[21],"providing":[22],"an":[23],"excellent":[24],"tool":[25],"for":[26,107,118],"research":[28,108],"viral":[30,46,58,167],"gene":[31,100],"functions":[32,112],"and":[33,71,113],"virus-host":[34],"interactions.":[35],"A":[36,143,154],"full-length":[37],"clone":[38],"can":[39],"be":[40],"in":[42,51,93,162,171],"vitro":[43],"vector":[47,88],"expressing":[48],"foreign":[49],"poly/oligopeptides":[50],"soluble":[52],"form":[53],"fused":[55],"capsid":[59],"protein.":[60],"The":[61,121],"use":[62,78],"plants":[65,165],"as":[66],"producers":[67],"antigens,":[69],"allergens,":[70],"other":[72,79],"pharmaceuticals":[73],"is":[74],"cheaper":[75],"than":[76],"eukaryotic":[80],"bioreactors.":[81],"Transient":[82],"expression":[83],"proteins":[85],"using":[86,166],"technique":[89],"several":[91],"advantages":[92],"comparison":[94],"with":[95],"transgenic":[97],"approach.":[98],"Virus-induced":[99],"silencing":[101],"vectors":[102,168],"have":[103],"been":[104],"widely":[105],"adapted":[106],"genes":[111],"screening":[114],"genomes":[117],"resistance":[119],"genes.":[120],"potential":[122],"instability":[123],"constructs":[126],"remains":[127],"main":[129],"problem":[130],"virus":[132,139],"vector-based":[133],"techniques":[134],"leading":[135],"wild-type":[138],"recovery":[140],"by":[141],"recombination.":[142],"novel":[144],"approach":[145],"including":[146],"\"a":[147],"deconstructed":[148],"virus-strategy\"":[149],"may":[150],"overcome":[151],"these":[152],"difficulties.":[153],"preparative-scale":[155],"production":[156],"numerous":[158],"biologically":[159],"active":[160],"compounds":[161],"various":[164],"are":[169],"expected":[170],"near":[173],"future.":[174]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}