{"id":"https://openalex.org/W1551951435","doi":"https://doi.org/10.1371/journal.pone.0130324","title":"Can DNA-Based Ecosystem Assessments Quantify Species Abundance? Testing Primer Bias and Biomass\u2014Sequence Relationships with an Innovative Metabarcoding Protocol","display_name":"Can DNA-Based Ecosystem Assessments Quantify Species Abundance? Testing Primer Bias and Biomass\u2014Sequence Relationships with an Innovative Metabarcoding Protocol","publication_year":2015,"publication_date":"2015-07-08","ids":{"openalex":"https://openalex.org/W1551951435","doi":"https://doi.org/10.1371/journal.pone.0130324","mag":"1551951435","pmid":"https://pubmed.ncbi.nlm.nih.gov/26154168"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0130324","doi":"https://doi.org/10.1371/journal.pone.0130324","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0130324","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0130324&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0130324&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006011875","display_name":"Vasco Elbrecht","orcid":"https://orcid.org/0000-0003-4672-7099","observed_orcids":["https://orcid.org/0000-0003-4672-7099"]},"institutions":[{"id":"https://openalex.org/I904495901","display_name":"Ruhr University Bochum","ror":"https://ror.org/04tsk2644","country_code":"DE","type":"education","lineage":["https://openalex.org/I904495901"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Vasco Elbrecht","raw_affiliation_strings":["Department of Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitaetsstrasse 150, D-44801 Bochum, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitaetsstrasse 150, D-44801 Bochum, Germany","institution_ids":["https://openalex.org/I904495901"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037731495","display_name":"Florian Leese","orcid":"https://orcid.org/0000-0002-5465-913X","observed_orcids":["https://orcid.org/0000-0002-5465-913X"]},"institutions":[{"id":"https://openalex.org/I904495901","display_name":"Ruhr University Bochum","ror":"https://ror.org/04tsk2644","country_code":"DE","type":"education","lineage":["https://openalex.org/I904495901"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Florian Leese","raw_affiliation_strings":["Department of Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitaetsstrasse 150, D-44801 Bochum, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitaetsstrasse 150, D-44801 Bochum, Germany","institution_ids":["https://openalex.org/I904495901"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5037731495"],"corresponding_institution_ids":["https://openalex.org/I904495901"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":54.8441,"has_fulltext":true,"cited_by_count":760,"citation_normalized_percentile":{"value":0.99967848,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":100},"biblio":{"volume":"10","issue":"7","first_page":"e0130324","last_page":"e0130324"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T14399","display_name":"Invertebrate Taxonomy and Ecology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-metabarcoding","display_name":"DNA metabarcoding","score":0.9599999785423279},{"id":"https://openalex.org/keywords/primer-bias","display_name":"primer bias","score":0.9380000233650208},{"id":"https://openalex.org/keywords/species-abundance-estimation","display_name":"species abundance estimation","score":0.8050000071525574},{"id":"https://openalex.org/keywords/coi-dna-barcoding","display_name":"COI barcoding","score":0.859000027179718},{"id":"https://openalex.org/keywords/freshwater-macroinvertebrates","display_name":"freshwater macroinvertebrates","score":0.996999979019165},{"id":"https://openalex.org/keywords/plecoptera","display_name":"Plecoptera","score":0.8220000267028809},{"id":"https://openalex.org/keywords/miseq-sequencing","display_name":"MiSeq sequencing","score":0.6290000081062317},{"id":"https://openalex.org/keywords/biodiversity-assessment","display_name":"biodiversity assessment","score":0.7179999947547913}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.764004647731781},{"id":"https://openalex.org/C141732470","wikidata":"https://www.wikidata.org/wiki/Q1154642","display_name":"DNA barcoding","level":2,"score":0.7439993023872375},{"id":"https://openalex.org/C2779969263","wikidata":"https://www.wikidata.org/wiki/Q25098939","display_name":"Environmental DNA","level":3,"score":0.5668478608131409},{"id":"https://openalex.org/C77077793","wikidata":"https://www.wikidata.org/wiki/Q336019","display_name":"Abundance (ecology)","level":2,"score":0.5233351588249207},{"id":"https://openalex.org/C115540264","wikidata":"https://www.wikidata.org/wiki/Q2945560","display_name":"Biomass (ecology)","level":2,"score":0.5159909725189209},{"id":"https://openalex.org/C130217890","wikidata":"https://www.wikidata.org/wiki/Q47041","display_name":"Biodiversity","level":2,"score":0.5072819590568542},{"id":"https://openalex.org/C2778200843","wikidata":"https://www.wikidata.org/wiki/Q14865314","display_name":"Cytochrome c oxidase subunit I","level":4,"score":0.47622424364089966},{"id":"https://openalex.org/C71640776","wikidata":"https://www.wikidata.org/wiki/Q16521","display_name":"Taxon","level":2,"score":0.47310709953308105},{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.4723179042339325},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.4579741656780243},{"id":"https://openalex.org/C122325731","wikidata":"https://www.wikidata.org/wiki/Q3738459","display_name":"Relative species abundance","level":3,"score":0.449860155582428},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.4276198744773865},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.39000654220581055},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.37821507453918457},{"id":"https://openalex.org/C90132467","wikidata":"https://www.wikidata.org/wiki/Q171184","display_name":"Phylogenetics","level":3,"score":0.2624582350254059},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.23499420285224915},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18634870648384094},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003576","descriptor_name":"Electron Transport Complex IV","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003576","descriptor_name":"Electron Transport Complex IV","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003576","descriptor_name":"Electron Transport Complex IV","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004175","descriptor_name":"Diptera","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D004175","descriptor_name":"Diptera","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D004175","descriptor_name":"Diptera","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D004175","descriptor_name":"Diptera","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004175","descriptor_name":"Diptera","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004175","descriptor_name":"Diptera","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005618","descriptor_name":"Fresh Water","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005618","descriptor_name":"Fresh Water","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005618","descriptor_name":"Fresh Water","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006239","descriptor_name":"Haplotypes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006239","descriptor_name":"Haplotypes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006239","descriptor_name":"Haplotypes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017753","descriptor_name":"Ecosystem","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017753","descriptor_name":"Ecosystem","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017753","descriptor_name":"Ecosystem","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018533","descriptor_name":"Biomass","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018533","descriptor_name":"Biomass","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018533","descriptor_name":"Biomass","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D044822","descriptor_name":"Biodiversity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D044822","descriptor_name":"Biodiversity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D044822","descriptor_name":"Biodiversity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D058893","descriptor_name":"DNA Barcoding, Taxonomic","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D058893","descriptor_name":"DNA Barcoding, Taxonomic","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D058893","descriptor_name":"DNA Barcoding, Taxonomic","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D063191","descriptor_name":"Odonata","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D063191","descriptor_name":"Odonata","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D063191","descriptor_name":"Odonata","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D063191","descriptor_name":"Odonata","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D063191","descriptor_name":"Odonata","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D063191","descriptor_name":"Odonata","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1371/journal.pone.0130324","doi":"https://doi.org/10.1371/journal.pone.0130324","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0130324","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0130324&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},{"id":"pmid:26154168","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26154168","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4496048","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4496048","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:37a78b5d23064c6686ed4e622c535b90","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/37a78b5d23064c6686ed4e622c535b90","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 10, Iss 7, p e0130324 (2015)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/1477268","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/_Can_DNA_Based_Ecosystem_Assessments_Quantify_Species_Abundance_Testing_Primer_Bias_and_Biomass_8212_Sequence_Relationships_with_an_Innovative_Metabarcoding_Protocol_/1477268","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0130324","doi":"https://doi.org/10.1371/journal.pone.0130324","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0130324","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0130324&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/15","score":0.6399999856948853,"display_name":"Life on land"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1551951435.pdf","grobid_xml":"https://content.openalex.org/works/W1551951435.grobid-xml"},"referenced_works_count":60,"referenced_works":["https://openalex.org/W1551533759","https://openalex.org/W1582907243","https://openalex.org/W1965466602","https://openalex.org/W1967106084","https://openalex.org/W1971811212","https://openalex.org/W1979060637","https://openalex.org/W1988661748","https://openalex.org/W1995628545","https://openalex.org/W2003510670","https://openalex.org/W2013445830","https://openalex.org/W2015053255","https://openalex.org/W2020200409","https://openalex.org/W2022251739","https://openalex.org/W2027589602","https://openalex.org/W2031550784","https://openalex.org/W2031849362","https://openalex.org/W2036897871","https://openalex.org/W2039104568","https://openalex.org/W2051331361","https://openalex.org/W2051569068","https://openalex.org/W2052929303","https://openalex.org/W2057300215","https://openalex.org/W2065838202","https://openalex.org/W2072970694","https://openalex.org/W2081858203","https://openalex.org/W2083709108","https://openalex.org/W2084608725","https://openalex.org/W2085525886","https://openalex.org/W2088833470","https://openalex.org/W2089848025","https://openalex.org/W2096885696","https://openalex.org/W2098534604","https://openalex.org/W2098869000","https://openalex.org/W2099901798","https://openalex.org/W2101462556","https://openalex.org/W2107140090","https://openalex.org/W2108718991","https://openalex.org/W2112732800","https://openalex.org/W2114259093","https://openalex.org/W2115439841","https://openalex.org/W2131347012","https://openalex.org/W2139394020","https://openalex.org/W2142678478","https://openalex.org/W2149415648","https://openalex.org/W2150433859","https://openalex.org/W2152854843","https://openalex.org/W2156780347","https://openalex.org/W2159396143","https://openalex.org/W2160438104","https://openalex.org/W2162578061","https://openalex.org/W2163407103","https://openalex.org/W2168866810","https://openalex.org/W2169286869","https://openalex.org/W2171894968","https://openalex.org/W2172820226","https://openalex.org/W2317481118","https://openalex.org/W2582743722","https://openalex.org/W3010621220","https://openalex.org/W3121953277","https://openalex.org/W6647360802"],"related_works":["https://openalex.org/W2130811337","https://openalex.org/W2261001172","https://openalex.org/W2159486495","https://openalex.org/W2980263927","https://openalex.org/W2367672784","https://openalex.org/W2974422654","https://openalex.org/W2399676914","https://openalex.org/W2626430079","https://openalex.org/W2147153142","https://openalex.org/W2730981817"],"abstract_inverted_index":{"Metabarcoding":[0],"is":[1,265],"an":[2,21],"emerging":[3],"genetic":[4],"tool":[5],"to":[6,26,38,96,188,226],"rapidly":[7],"assess":[8],"biodiversity":[9,286],"in":[10,74,104,107,120,156,278],"ecosystems.":[11],"It":[12],"involves":[13],"high-throughput":[14,256],"sequencing":[15,141],"of":[16,53,133,144,154,165,171,194,213,221,237,244,247,273,285],"a":[17,27,63,80,108,145,203,279],"standard":[18,87,177],"gene":[19],"from":[20,46],"environmental":[22,47],"sample":[23],"and":[24,42,56,78,112,114,210,276],"comparison":[25],"reference":[28],"database.":[29],"However,":[30,230,258],"no":[31],"consensus":[32],"has":[33,66],"emerged":[34],"regarding":[35],"laboratory":[36],"pipelines":[37],"screen":[39],"species":[40,44,147,208,274],"diversity":[41],"infer":[43],"abundances":[45],"samples.":[48],"In":[49],"particular,":[50],"the":[51,57,86,115,131,163,175,222,242,259],"effect":[52],"primer":[54,228,263],"bias":[55],"detection":[58],"limit":[59],"for":[60,255],"specimens":[61,143],"with":[62,148],"low":[64],"biomass":[65,135,157,173,209,277],"not":[67],"been":[68],"systematically":[69],"examined,":[70],"when":[71],"processing":[72],"samples":[73],"bulk.":[75],"We":[76,201],"developed":[77],"tested":[79,162],"DNA":[81,101],"metabarcoding":[82,250,283],"protocol":[83],"that":[84,262],"utilises":[85],"cytochrome":[88],"c":[89],"oxidase":[90],"subunit":[91],"I":[92],"(COI)":[93],"barcoding":[94,178],"fragment":[95],"detect":[97],"freshwater":[98,168],"macroinvertebrate":[99],"taxa.":[100],"was":[102,184],"extracted":[103],"bulk,":[105],"amplified":[106],"single":[109],"PCR":[110],"step,":[111],"purified,":[113],"libraries":[116],"were":[117,197],"directly":[118],"sequenced":[119],"two":[121],"independent":[122],"MiSeq":[123,214],"runs":[124],"(300-bp":[125],"paired-end":[126],"reads).":[127],"Specifically,":[128],"we":[129,161,217],"assessed":[130],"influence":[132],"specimen":[134],"on":[136,289],"sequence":[137,231],"read":[138],"abundance":[139,232,275],"by":[140,234],"31":[142],"stonefly":[146],"known":[149],"haplotypes":[150],"spanning":[151],"three":[152],"orders":[153,236],"magnitude":[155],"(experiment":[158,180],"I).":[159],"Then,":[160],"recovery":[164],"52":[166,223],"different":[167],"invertebrate":[169],"taxa":[170,224,240],"similar":[172,245],"using":[174],"same":[176],"primers":[179],"II).":[181],"Each":[182],"experiment":[183],"replicated":[185],"ten":[186],"times":[187],"maximise":[189],"statistical":[190],"power.":[191],"The":[192],"results":[193,254,260],"both":[195],"experiments":[196],"consistent":[198],"across":[199],"replicates.":[200],"found":[202],"distinct":[204],"positive":[205],"correlation":[206],"between":[207,239],"resulting":[211],"numbers":[212],"reads.":[215],"Furthermore,":[216],"reliably":[218],"recovered":[219],"83%":[220],"used":[225],"test":[227],"bias.":[229],"varied":[233],"four":[235],"magnitudes":[238],"despite":[241],"use":[243],"amounts":[246],"biomass.":[248],"Our":[249],"approach":[251],"yielded":[252],"reliable":[253],"assessments.":[257],"indicated":[261],"efficiency":[264],"highly":[266],"species-specific,":[267],"which":[268],"would":[269],"prevent":[270],"straightforward":[271],"assessments":[272,284],"sample.":[280],"Thus,":[281],"PCR-based":[282],"should":[287],"rely":[288],"presence-absence":[290],"metrics.":[291]},"counts_by_year":[{"year":2026,"cited_by_count":71},{"year":2025,"cited_by_count":73},{"year":2024,"cited_by_count":49},{"year":2023,"cited_by_count":69},{"year":2022,"cited_by_count":71},{"year":2021,"cited_by_count":91},{"year":2020,"cited_by_count":75},{"year":2019,"cited_by_count":73},{"year":2018,"cited_by_count":108},{"year":2017,"cited_by_count":51},{"year":2016,"cited_by_count":26},{"year":2015,"cited_by_count":3}],"updated_date":"2026-10-17T09:40:24.721904","created_date":"2016-06-24T00:00:00"}
