{"id":"https://openalex.org/W1551410107","doi":"https://doi.org/10.1016/s0021-9258(19)85032-9","title":"Purification of a Protein from Bovine Plasma That Binds to Type 1 Plasminogen Activator Inhibitor and Prevents Its Interaction with Extracellular Matrix","display_name":"Purification of a Protein from Bovine Plasma That Binds to Type 1 Plasminogen Activator Inhibitor and Prevents Its Interaction with Extracellular Matrix","publication_year":1989,"publication_date":"1989-01-01","ids":{"openalex":"https://openalex.org/W1551410107","doi":"https://doi.org/10.1016/s0021-9258(19)85032-9","mag":"1551410107","pmid":"https://pubmed.ncbi.nlm.nih.gov/2463252"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)85032-9","doi":"https://doi.org/10.1016/s0021-9258(19)85032-9","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)85032-9","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)85032-9","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012340452","display_name":"Jun Mimuro","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210146328","display_name":"Scripps Clinic","ror":"https://ror.org/05kwjwj05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1311914864","https://openalex.org/I4210146328"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Mimuro","raw_affiliation_strings":["Department of Immunology Research Institute of Scripps Clinic La Jolla, California 92037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Immunology Research Institute of Scripps Clinic La Jolla, California 92037","institution_ids":["https://openalex.org/I4210146328"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077803529","display_name":"David J. Loskutoff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210146328","display_name":"Scripps Clinic","ror":"https://ror.org/05kwjwj05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1311914864","https://openalex.org/I4210146328"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D J Loskutoff","raw_affiliation_strings":["Department of Immunology Research Institute of Scripps Clinic La Jolla, California 92037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Immunology Research Institute of Scripps Clinic La Jolla, California 92037","institution_ids":["https://openalex.org/I4210146328"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210146328"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":13.9133,"has_fulltext":false,"cited_by_count":195,"citation_normalized_percentile":{"value":0.99240654,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"264","issue":"2","first_page":"936","last_page":"939"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.6563000082969666,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.6563000082969666,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.22419999539852142,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.09650000184774399,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasminogen-activator-inhibitor-1","display_name":"plasminogen activator inhibitor-1","score":0.968999981880188},{"id":"https://openalex.org/keywords/vitronectin","display_name":"vitronectin","score":0.9580000042915344},{"id":"https://openalex.org/keywords/extracellular-matrix","display_name":"extracellular matrix","score":0.753000020980835},{"id":"https://openalex.org/keywords/bovine-plasma","display_name":"bovine plasma","score":0.7049999833106995},{"id":"https://openalex.org/keywords/bovine-aortic-endothelial-cells","display_name":"bovine aortic endothelial cells","score":0.7870000004768372},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.777999997138977},{"id":"https://openalex.org/keywords/nh2-terminal-sequence","display_name":"NH2-terminal sequence","score":0.8059999942779541},{"id":"https://openalex.org/keywords/lung","display_name":"lung","score":0.4099999964237213},{"id":"https://openalex.org/keywords/fibrinolysis","display_name":"fibrinolysis","score":0.5619999766349792}],"concepts":[{"id":"https://openalex.org/C2779679481","wikidata":"https://www.wikidata.org/wiki/Q13581432","display_name":"Plasminogen activator","level":2,"score":0.7387404441833496},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.6561837792396545},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5625841617584229},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.5386474132537842},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.44910144805908203},{"id":"https://openalex.org/C88045685","wikidata":"https://www.wikidata.org/wiki/Q174576","display_name":"Activator (genetics)","level":3,"score":0.44475576281547546},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22131094336509705},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.11270484328269958},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.06664377450942993}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010960","descriptor_name":"Plasminogen Activators","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D010960","descriptor_name":"Plasminogen Activators","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D010960","descriptor_name":"Plasminogen Activators","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015849","descriptor_name":"Plasminogen Inactivators","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015849","descriptor_name":"Plasminogen Inactivators","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015849","descriptor_name":"Plasminogen Inactivators","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019096","descriptor_name":"Vitronectin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019096","descriptor_name":"Vitronectin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019096","descriptor_name":"Vitronectin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)85032-9","doi":"https://doi.org/10.1016/s0021-9258(19)85032-9","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)85032-9","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2463252","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2463252","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)85032-9","doi":"https://doi.org/10.1016/s0021-9258(19)85032-9","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)85032-9","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2050139205","display_name":null,"funder_award_id":"HL16411","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G3835578748","display_name":null,"funder_award_id":"HL22289","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W160172706","https://openalex.org/W210988120","https://openalex.org/W292647304","https://openalex.org/W1484931749","https://openalex.org/W1511176863","https://openalex.org/W1550499229","https://openalex.org/W1569056261","https://openalex.org/W1571398079","https://openalex.org/W1579151760","https://openalex.org/W1863234733","https://openalex.org/W1893939249","https://openalex.org/W1932052686","https://openalex.org/W1964171893","https://openalex.org/W1969361987","https://openalex.org/W1983503063","https://openalex.org/W1986612369","https://openalex.org/W2014316985","https://openalex.org/W2033873544","https://openalex.org/W2052818567","https://openalex.org/W2080185693","https://openalex.org/W2100837269","https://openalex.org/W2115715429","https://openalex.org/W2119460480","https://openalex.org/W2158431724","https://openalex.org/W2408590235","https://openalex.org/W2409062223","https://openalex.org/W2466118865"],"related_works":["https://openalex.org/W2003533505","https://openalex.org/W2155133540","https://openalex.org/W1601891991","https://openalex.org/W1595622217","https://openalex.org/W1493779021","https://openalex.org/W2079723138","https://openalex.org/W299413942","https://openalex.org/W1548286725","https://openalex.org/W2050325685","https://openalex.org/W1536441019"],"abstract_inverted_index":{"Type":[0],"1":[1],"plasminogen":[2],"activator":[3],"inhibitor":[4],"(PAI-1)":[5],"binds":[6],"to":[7,27],"the":[8,81,111,123],"extracellular":[9,124],"matrix":[10,125],"of":[11,34,59,63,70,80,126],"cultured":[12],"bovine":[13,20,127,133],"aortic":[14],"endothelial":[15,128],"cells.":[16],"Bovine":[17],"plasma":[18,44],"and":[19,29,66,73,92,101,117,130],"lung":[21],"extract":[22],"contain":[23],"protein(s)":[24],"that":[25,86,110,118],"bind":[26],"PAI-1":[28],"prevent":[30],"this":[31,95],"interaction.":[32],"One":[33],"these":[35,107],"proteins":[36],"was":[37,88,120],"purified":[38,96],"approximately":[39],"425-fold":[40],"from":[41],"ammonium":[42],"sulfate-fractionated":[43],"using":[45,106],"standard":[46],"chromatographic":[47],"procedures":[48],"together":[49],"with":[50,90],"affinity":[51],"chromatography":[52],"on":[53],"PAI-1-Sepharose.":[54],"The":[55],"final":[56],"product":[57],"consisted":[58],"a":[60],"major":[61],"polypeptide":[62,84],"Mr":[64,71,82],"65,000":[65,83],"two":[67],"minor":[68],"polypeptides":[69],"80,000":[72],"57,000.":[74],"NH2-terminal":[75],"amino":[76],"acid":[77],"sequence":[78],"analysis":[79,105],"revealed":[85],"it":[87],"homologous":[89],"vitronectin,":[91],"antiserum":[93],"against":[94],"binding":[97],"protein":[98],"recognized":[99],"vitronectin":[100,119],"vice":[102],"versa.":[103],"Immunological":[104],"antisera":[108],"demonstrated":[109],"three":[112],"peptides":[113],"were":[114],"immunologically":[115],"related,":[116],"present":[121],"in":[122,132],"cells":[129],"also":[131],"lung.":[134]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-06-24T00:00:00"}