{"id":"https://openalex.org/W1551254087","doi":"https://doi.org/10.1016/s0021-9258(18)99166-0","title":"Mechanism of the sliding beta-clamp of DNA polymerase III holoenzyme","display_name":"Mechanism of the sliding beta-clamp of DNA polymerase III holoenzyme","publication_year":1991,"publication_date":"1991-06-01","ids":{"openalex":"https://openalex.org/W1551254087","doi":"https://doi.org/10.1016/s0021-9258(18)99166-0","mag":"1551254087","pmid":"https://pubmed.ncbi.nlm.nih.gov/2040637"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)99166-0","doi":"https://doi.org/10.1016/s0021-9258(18)99166-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)99166-0","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)99166-0","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072633316","display_name":"P. Todd Stukenberg","orcid":"https://orcid.org/0000-0002-6788-2111","observed_orcids":["https://orcid.org/0000-0002-6788-2111"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P.T. Stukenberg","raw_affiliation_strings":["Howard Hughes Medical Institute, Hearst Microbiology Department, Cornell University Medical College, New York, New York 10021"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Hearst Microbiology Department, Cornell University Medical College, New York, New York 10021","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080031372","display_name":"P.S. Studwell-Vaughan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P.S. Studwell-Vaughan","raw_affiliation_strings":["Howard Hughes Medical Institute, Hearst Microbiology Department, Cornell University Medical College, New York, New York 10021"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Hearst Microbiology Department, Cornell University Medical College, New York, New York 10021","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051770888","display_name":"Mike E. O\u2019Donnell","orcid":"https://orcid.org/0000-0001-9002-4214","observed_orcids":["https://orcid.org/0000-0001-9002-4214","https://orcid.org/0000-0002-8928-8068"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. O'Donnell","raw_affiliation_strings":["Howard Hughes Medical Institute, Hearst Microbiology Department, Cornell University Medical College, New York, New York 10021"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Hearst Microbiology Department, Cornell University Medical College, New York, New York 10021","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I205783295"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":8.9879,"has_fulltext":false,"cited_by_count":413,"citation_normalized_percentile":{"value":0.98759494,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"266","issue":"17","first_page":"11328","last_page":"11334"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.43380001187324524,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.43380001187324524,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.40380001068115234,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13187","display_name":"Diffusion and Search Dynamics","score":0.09220000356435776,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-polymerase-iii-holoenzyme","display_name":"DNA polymerase III holoenzyme","score":0.996999979019165},{"id":"https://openalex.org/keywords/beta-sliding-clamp","display_name":"beta sliding clamp","score":0.8610000014305115},{"id":"https://openalex.org/keywords/dna-polymerase-processivity","display_name":"DNA polymerase processivity","score":0.7279999852180481},{"id":"https://openalex.org/keywords/dna-replication","display_name":"DNA replication","score":0.4749999940395355},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8539999723434448},{"id":"https://openalex.org/keywords/dna-binding-specificity","display_name":"DNA binding specificity","score":0.3610000014305115}],"concepts":[{"id":"https://openalex.org/C31254050","wikidata":"https://www.wikidata.org/wiki/Q22328799","display_name":"DNA clamp","level":5,"score":0.7092677354812622},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.6952335834503174},{"id":"https://openalex.org/C2776161997","wikidata":"https://www.wikidata.org/wiki/Q846600","display_name":"Clamp","level":3,"score":0.613021194934845},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5157899856567383},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4829398989677429},{"id":"https://openalex.org/C2778462844","wikidata":"https://www.wikidata.org/wiki/Q18030641","display_name":"DNA polymerase beta","level":5,"score":0.4716065526008606},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.46250221133232117},{"id":"https://openalex.org/C2776174256","wikidata":"https://www.wikidata.org/wiki/Q830842","display_name":"BETA (programming language)","level":2,"score":0.4321382939815521},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3982309103012085},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3119274973869324},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28540152311325073},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.27882903814315796},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26004117727279663},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.20304608345031738},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19025051593780518},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.18065863847732544},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.053954094648361206},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.0},{"id":"https://openalex.org/C187206112","wikidata":"https://www.wikidata.org/wiki/Q3113091","display_name":"Base excision repair","level":4,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C84111939","wikidata":"https://www.wikidata.org/wiki/Q5125465","display_name":"Clamping","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D004258","descriptor_name":"DNA Polymerase III","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004258","descriptor_name":"DNA Polymerase III","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004258","descriptor_name":"DNA Polymerase III","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013698","descriptor_name":"Templates, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046912","descriptor_name":"Multiprotein Complexes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)99166-0","doi":"https://doi.org/10.1016/s0021-9258(18)99166-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)99166-0","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2040637","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2040637","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)99166-0","doi":"https://doi.org/10.1016/s0021-9258(18)99166-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)99166-0","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1443451985","display_name":null,"funder_award_id":"GM 38839","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W1484906518","https://openalex.org/W1492751534","https://openalex.org/W1493097357","https://openalex.org/W1513717105","https://openalex.org/W1531749456","https://openalex.org/W1534129180","https://openalex.org/W1535209874","https://openalex.org/W1535843470","https://openalex.org/W1540815504","https://openalex.org/W1541300405","https://openalex.org/W1548939450","https://openalex.org/W1554040058","https://openalex.org/W1555603967","https://openalex.org/W1559132509","https://openalex.org/W1562566862","https://openalex.org/W1567442633","https://openalex.org/W1568597106","https://openalex.org/W1572403368","https://openalex.org/W1582244872","https://openalex.org/W1605482400","https://openalex.org/W1653821563","https://openalex.org/W1957748504","https://openalex.org/W1965743560","https://openalex.org/W1985971484","https://openalex.org/W2015221134","https://openalex.org/W2026741378","https://openalex.org/W2031645882","https://openalex.org/W2035650458","https://openalex.org/W2059164909","https://openalex.org/W2079718256","https://openalex.org/W2092713502","https://openalex.org/W2100837269","https://openalex.org/W2128635872","https://openalex.org/W2132897827","https://openalex.org/W2144634347"],"related_works":["https://openalex.org/W75099420","https://openalex.org/W2009509451","https://openalex.org/W2065014822","https://openalex.org/W1492751534","https://openalex.org/W1571040374","https://openalex.org/W1519811573","https://openalex.org/W2007433956","https://openalex.org/W2041249795","https://openalex.org/W2048933338","https://openalex.org/W1531749456"],"abstract_inverted_index":{"DNA":[0,16,124,157,176],"polymerase":[1,71,151,158,180],"III":[2],"holoenzyme":[3,166],"(holoenzyme),":[4],"the":[5,28,47,64,87,94,98,105,111,142,148,156,161,165,179,182],"multiprotein":[6],"replicase":[7],"of":[8,121,164,173],"Escherichia":[9],"coli,":[10],"is":[11,32,44,63,73,77,125,130,167],"essentially":[12],"unlimited":[13],"in":[14,169],"processive":[15,82],"synthesis.":[17],"Processive":[18],"activity":[19,83],"can":[20],"be":[21,116],"reconstituted":[22],"from":[23],"two":[24],"components.":[25],"One":[26],"component,":[27],"beta":[29,34,41,56,88,99,112,122,145,174],"preinitiation":[30,42,89,100,113],"complex,":[31,50],"a":[33,170],"dimer":[35],"clamped":[36],"onto":[37,57,104],"primed":[38,58,183],"DNA.":[39,59,119],"The":[40,60,68],"complex":[43,101,114],"formed":[45],"by":[46,96],"five-protein":[48],"gamma":[49],"which":[51,97],"hydrolyzes":[52],"ATP":[53],"to":[54,115,127,138,181],"chaperone":[55],"other":[61],"component":[62],"alpha":[65,69,106,149],"epsilon":[66,70,107,150],"polymerase.":[67,108],"itself":[72],"not":[74,133],"processive,":[75],"but":[76],"endowed":[78],"with":[79,86,154],"extremely":[80],"high":[81,162],"upon":[84],"assembly":[85],"complex.":[90],"Here":[91],"we":[92],"examine":[93],"mechanism":[95],"confers":[102],"processivity":[103,163],"We":[109],"find":[110],"mobile":[117],"on":[118,123,175],"Diffusion":[120],"specific":[126],"duplex":[128],"DNA,":[129],"bidirectional,":[131],"does":[132],"require":[134],"ATP,":[135],"and":[136,188],"appears":[137],"diffuse":[139],"linearly":[140],"along":[141],"duplex.":[143],"Furthermore,":[144],"directly":[146],"binds":[147],"through":[152],"contact":[153],"alpha,":[155],"subunit.":[159],"Hence,":[160],"rooted":[168],"\"sliding":[171],"clamp\"":[172],"that":[177],"tethers":[178],"template.":[184],"Implications":[185],"for":[186],"transcription":[187],"translation":[189],"are":[190],"discussed.":[191]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":9},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":13},{"year":2018,"cited_by_count":12},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":14},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":10},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":8}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}