{"id":"https://openalex.org/W1550018201","doi":"https://doi.org/10.1128/jcm.29.11.2461-2467.1991","title":"Evaluation of infection with human immunodeficiency virus type 1 by using nonisotopic solution hybridization for detection of polymerase chain reaction-amplified proviral DNA","display_name":"Evaluation of infection with human immunodeficiency virus type 1 by using nonisotopic solution hybridization for detection of polymerase chain reaction-amplified proviral DNA","publication_year":1991,"publication_date":"1991-11-01","ids":{"openalex":"https://openalex.org/W1550018201","doi":"https://doi.org/10.1128/jcm.29.11.2461-2467.1991","mag":"1550018201","pmid":"https://pubmed.ncbi.nlm.nih.gov/1774250","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/270355"},"language":"en","primary_location":{"id":"doi:10.1128/jcm.29.11.2461-2467.1991","doi":"https://doi.org/10.1128/jcm.29.11.2461-2467.1991","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jcm.29.11.2461-2467.1991","pdf_url":null,"source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC270355","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081455279","display_name":"Fran\u00e7ois Coutl\u00e9e","orcid":"https://orcid.org/0000-0002-2025-8928","observed_orcids":["https://orcid.org/0000-0002-2025-8928","https://orcid.org/0000-0003-0507-3236"]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"F Coutl\u00e9e","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007711260","display_name":"Pierre Saint\u2013Antoine","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"P Saint-Antoine","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"middle","author":{"id":null,"display_name":"C Olivier","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"C Olivier","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034694167","display_name":"H\u00e9l\u00e8ne Voyer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"H Voyer","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047323770","display_name":"A Kessous","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"A Kessous-Elbaz","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012204353","display_name":"Fouad Berrada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"F Berrada","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049211681","display_name":"Paul B\u00e9gin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"P B\u00e9gin","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111973146","display_name":"Lise Giroux","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"L Giroux","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024057833","display_name":"Raphael P. Viscidi","orcid":"https://orcid.org/0000-0002-7906-8002","observed_orcids":["https://orcid.org/0000-0002-7906-8002"]},"institutions":[{"id":"https://openalex.org/I4210107362","display_name":"H\u00f4pital Notre-Dame","ror":"https://ror.org/01w7qz648","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801902503","https://openalex.org/I4210107362","https://openalex.org/I4210139015","https://openalex.org/I4210142730","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"R Viscidi","raw_affiliation_strings":["Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Infectious Diseases, Hopital Notre-Dame, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210107362"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210107362"],"apc_list":null,"apc_paid":null,"fwci":1.2758,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.76432917,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"29","issue":"11","first_page":"2461","last_page":"2467"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10082","display_name":"HIV/AIDS Research and Interventions","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hiv-1","display_name":"HIV-1","score":0.7960000038146973},{"id":"https://openalex.org/keywords/solution-hybridization","display_name":"solution hybridization","score":0.8949999809265137},{"id":"https://openalex.org/keywords/rna-dna-hybrids","display_name":"RNA-DNA hybrids","score":0.8550000190734863},{"id":"https://openalex.org/keywords/gag-gene","display_name":"gag gene","score":0.6389999985694885},{"id":"https://openalex.org/keywords/primer-specificity","display_name":"primer specificity","score":0.38600000739097595},{"id":"https://openalex.org/keywords/peripheral-blood-mononuclear-cells","display_name":"peripheral blood mononuclear cells","score":0.953000009059906}],"concepts":[{"id":"https://openalex.org/C102906071","wikidata":"https://www.wikidata.org/wiki/Q422467","display_name":"Provirus","level":4,"score":0.8597240447998047},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.8226458430290222},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7282946109771729},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.7085973024368286},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6557977199554443},{"id":"https://openalex.org/C2780420688","wikidata":"https://www.wikidata.org/wiki/Q759183","display_name":"Immunoassay","level":3,"score":0.6151549220085144},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5516001582145691},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.4987349510192871},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.49436140060424805},{"id":"https://openalex.org/C204628709","wikidata":"https://www.wikidata.org/wiki/Q864854","display_name":"Biotinylation","level":2,"score":0.49203726649284363},{"id":"https://openalex.org/C2778810118","wikidata":"https://www.wikidata.org/wiki/Q534260","display_name":"Microtiter plate","level":2,"score":0.4660927355289459},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.42588526010513306},{"id":"https://openalex.org/C52001828","wikidata":"https://www.wikidata.org/wiki/Q2902493","display_name":"Primer dimer","level":5,"score":0.42167115211486816},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.41344591975212097},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.39222452044487},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.23509982228279114},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1957608461380005},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.17102938890457153},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.07894536852836609},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.05728846788406372},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001710","descriptor_name":"Biotin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007124","descriptor_name":"Immunoenzyme Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011533","descriptor_name":"Proviruses","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011533","descriptor_name":"Proviruses","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D015347","descriptor_name":"RNA Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015497","descriptor_name":"HIV-1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015658","descriptor_name":"HIV Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D015658","descriptor_name":"HIV Infections","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/jcm.29.11.2461-2467.1991","doi":"https://doi.org/10.1128/jcm.29.11.2461-2467.1991","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jcm.29.11.2461-2467.1991","pdf_url":null,"source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:230165","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC270355","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:270355","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/270355","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Microbiol","raw_type":"Text"},{"id":"pmid:1774250","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1774250","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of clinical microbiology","raw_type":"Comparative Study"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:230165","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC270355","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W1485928263","https://openalex.org/W1519217882","https://openalex.org/W1629931862","https://openalex.org/W1871201083","https://openalex.org/W1929514407","https://openalex.org/W1945324701","https://openalex.org/W1974590216","https://openalex.org/W1976736675","https://openalex.org/W1977923433","https://openalex.org/W1979388953","https://openalex.org/W1981931294","https://openalex.org/W1987580201","https://openalex.org/W2002473487","https://openalex.org/W2007938471","https://openalex.org/W2026242634","https://openalex.org/W2032118018","https://openalex.org/W2033249788","https://openalex.org/W2034951085","https://openalex.org/W2047152679","https://openalex.org/W2047480444","https://openalex.org/W2053107576","https://openalex.org/W2056224227","https://openalex.org/W2059816057","https://openalex.org/W2074747188","https://openalex.org/W2078119356","https://openalex.org/W2081010694","https://openalex.org/W2084892947","https://openalex.org/W2095438774","https://openalex.org/W2101588264","https://openalex.org/W2114738695","https://openalex.org/W2117149353","https://openalex.org/W2144634347","https://openalex.org/W2146301733","https://openalex.org/W2147243442","https://openalex.org/W2149897099","https://openalex.org/W2242391508","https://openalex.org/W2475714682","https://openalex.org/W2795740701","https://openalex.org/W2893042394"],"related_works":["https://openalex.org/W1574646440","https://openalex.org/W2033249788","https://openalex.org/W2067256642","https://openalex.org/W2247372595","https://openalex.org/W2186277914","https://openalex.org/W2100442546","https://openalex.org/W2090933085","https://openalex.org/W1487337898","https://openalex.org/W1574056158","https://openalex.org/W1972816015"],"abstract_inverted_index":{"A":[0],"convenient":[1],"assay":[2,227],"combining":[3],"solution":[4,69],"hybridization":[5,37],"and":[6,32,86,127,138,140,149,182],"enzyme":[7],"immunoassay":[8,15],"for":[9,201,228],"DNA-RNA":[10,92],"hybrids":[11,76],"(polymerase":[12],"chain":[13],"reaction-enzyme":[14],"[PCR-EIA])":[16],"was":[17,33,55,66,113,153,175,209],"developed":[18],"to":[19,91,215,222],"detect":[20],"human":[21],"immunodeficiency":[22],"virus":[23],"type":[24],"1":[25],"(HIV-1)":[26],"provirus":[27,152],"amplified":[28,57,231],"by":[29,102,104,124,135,146,160],"the":[30,46,193,207],"PCR":[31,180,200],"compared":[34],"with":[35,38,58,70,83],"oligomer":[36,128],"32P-labeled":[39],"SK19.":[40],"In":[41],"PCR-EIA,":[42,139],"a":[43,71,80,87,162,172,199],"fragment":[44],"of":[45,96,109,117,132,143,157,164,167,192,224,230],"HIV-1":[47,97,111,151],"gag":[48],"gene":[49],"from":[50,171],"peripheral":[51,119],"blood":[52,120],"mononuclear":[53,121],"cells":[54],"first":[56],"primer":[59,189],"pair":[60],"SK38/SK39":[61,137],"or":[62],"O1/O2.":[63],"PCR-amplified":[64],"material":[65],"reacted":[67],"in":[68,79,115,130,141,155,177,198],"biotinylated":[72],"RNA":[73],"probe.":[74],"Biotinylated":[75],"were":[77],"measured":[78],"microtiter-plate":[81],"EIA":[82],"antibiotin":[84],"antibody":[85,90],"beta-D-galactosidase-conjugated":[88],"monoclonal":[89],"hybrids.":[93],"Ten":[94],"copies":[95],"DNA":[98,112],"could":[99],"be":[100],"detected":[101,114,154],"PCR-EIA":[103,218],"using":[105,125,136,147,161],"two":[106,165],"different":[107],"sets":[108,166],"primers.":[110,168],"104":[116,131,142],"108":[118,133,144,158],"cell":[122],"samples":[123,134,145,159,184,195],"SK38/39":[126],"hybridization,":[129],"O1/O2":[148],"PCR-EIA.":[150],"107":[156],"combination":[163],"One":[169],"sample":[170,208],"seropositive":[173],"patient":[174],"negative":[176,197],"all":[178],"three":[179],"assays,":[181],"six":[183],"gave":[185],"discordant":[186],"results":[187,220],"between":[188],"pairs.":[190],"Six":[191],"latter":[194],"scored":[196],"beta-globin":[202],"but":[203],"became":[204],"positive":[205],"when":[206],"diluted":[210],"before":[211],"amplification.":[212],"When":[213],"applied":[214],"clinical":[216],"samples,":[217],"generated":[219],"similar":[221],"those":[223],"an":[225],"isotopic":[226],"detection":[229],"DNA.":[232]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}