{"id":"https://openalex.org/W1548752314","doi":null,"title":"Processing of a precursor of 72-kilodalton type IV collagenase/gelatinase A by a recombinant membrane-type 1 matrix metalloproteinase.","display_name":"Processing of a precursor of 72-kilodalton type IV collagenase/gelatinase A by a recombinant membrane-type 1 matrix metalloproteinase.","publication_year":1996,"publication_date":"1996-06-01","ids":{"openalex":"https://openalex.org/W1548752314","mag":"1548752314","pmid":"https://pubmed.ncbi.nlm.nih.gov/8653693"},"language":"en","primary_location":{"id":"pmid:8653693","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8653693","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015166554","display_name":"Takeshi Kinoshita","orcid":"https://orcid.org/0000-0002-1290-0650","observed_orcids":["https://orcid.org/0000-0002-1290-0650"]},"institutions":[{"id":"https://openalex.org/I10091056","display_name":"Kanazawa University","ror":"https://ror.org/02hwp6a56","country_code":"JP","type":"education","lineage":["https://openalex.org/I10091056"]},{"id":"https://openalex.org/I4405286179","display_name":"Cancer Research Institute of Kanazawa University","ror":"https://ror.org/01bve4846","country_code":"JP","type":"facility","lineage":["https://openalex.org/I10091056","https://openalex.org/I4405286179"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"T Kinoshita","raw_affiliation_strings":["Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Japan","institution_ids":["https://openalex.org/I10091056","https://openalex.org/I4405286179"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112712564","display_name":"Hiroshi Sato","orcid":"https://orcid.org/0009-0005-6092-6037","observed_orcids":["https://orcid.org/0009-0005-6092-6037"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Sato","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111532327","display_name":"Takahisa Takino","orcid":"https://orcid.org/0009-0003-8242-3110","observed_orcids":["https://orcid.org/0009-0003-8242-3110"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Takino","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021776714","display_name":"Michiyasu Itoh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Itoh","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055868327","display_name":"Toshifumi Akizawa","orcid":"https://orcid.org/0000-0002-3440-0905","observed_orcids":["https://orcid.org/0000-0002-3440-0905"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Akizawa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5008902984","display_name":"Motoharu Seiki","orcid":"https://orcid.org/0000-0002-3909-0782","observed_orcids":["https://orcid.org/0000-0002-3909-0782"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Seiki","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":6.7439,"has_fulltext":false,"cited_by_count":111,"citation_normalized_percentile":{"value":0.97638285,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"56","issue":"11","first_page":"2535","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mt1-mmp","display_name":"MT1-MMP","score":0.9459999799728394},{"id":"https://openalex.org/keywords/mmp-2","display_name":"MMP-2","score":0.8679999709129333},{"id":"https://openalex.org/keywords/propeptide-cleavage","display_name":"propeptide cleavage","score":0.847000002861023},{"id":"https://openalex.org/keywords/type-iv-collagenase","display_name":"type IV collagenase","score":0.7910000085830688},{"id":"https://openalex.org/keywords/proteolytic-activation","display_name":"proteolytic activation","score":0.8159999847412109},{"id":"https://openalex.org/keywords/timp-2","display_name":"TIMP-2","score":0.9259999990463257},{"id":"https://openalex.org/keywords/batimastat","display_name":"batimastat","score":0.906000018119812},{"id":"https://openalex.org/keywords/recombinant-fusion-protein","display_name":"recombinant fusion protein","score":0.7540000081062317},{"id":"https://openalex.org/keywords/sequence-specific-cleavage","display_name":"sequence-specific cleavage","score":0.4690000116825104}],"concepts":[{"id":"https://openalex.org/C109523444","wikidata":"https://www.wikidata.org/wiki/Q424216","display_name":"Matrix metalloproteinase","level":2,"score":0.8540121912956238},{"id":"https://openalex.org/C2776223131","wikidata":"https://www.wikidata.org/wiki/Q13033733","display_name":"Gelatinase A","level":3,"score":0.8106304407119751},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7458242177963257},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.7072957158088684},{"id":"https://openalex.org/C2776232661","wikidata":"https://www.wikidata.org/wiki/Q5530373","display_name":"Gelatinase","level":3,"score":0.7032133936882019},{"id":"https://openalex.org/C55728118","wikidata":"https://www.wikidata.org/wiki/Q425402","display_name":"Metalloproteinase","level":3,"score":0.6878907084465027},{"id":"https://openalex.org/C2778394429","wikidata":"https://www.wikidata.org/wiki/Q304956","display_name":"Collagenase","level":3,"score":0.6069567203521729},{"id":"https://openalex.org/C2776747403","wikidata":"https://www.wikidata.org/wiki/Q13033710","display_name":"Interstitial collagenase","level":4,"score":0.5157508850097656},{"id":"https://openalex.org/C2775958869","wikidata":"https://www.wikidata.org/wiki/Q16966320","display_name":"Matrix metalloproteinase inhibitor","level":3,"score":0.49549758434295654},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4883989691734314},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4762696921825409},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.45167699456214905},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.44787225127220154},{"id":"https://openalex.org/C7860815","wikidata":"https://www.wikidata.org/wiki/Q258658","display_name":"Protein precursor","level":3,"score":0.4140346646308899},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3729328513145447},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33155226707458496},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09004399180412292}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002522","descriptor_name":"Chlorocebus aethiops","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008666","descriptor_name":"Metalloendopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011480","descriptor_name":"Protease Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011499","descriptor_name":"Protein Processing, Post-Translational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013876","descriptor_name":"Thiophenes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017364","descriptor_name":"Collagenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018093","descriptor_name":"Gelatinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019714","descriptor_name":"Tissue Inhibitor of Metalloproteinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019716","descriptor_name":"Tissue Inhibitor of Metalloproteinase-2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020778","descriptor_name":"Matrix Metalloproteinase 2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020781","descriptor_name":"Matrix Metalloproteinase 1","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D061965","descriptor_name":"Matrix Metalloproteinase Inhibitors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:8653693","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8653693","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},{"id":"mag:1548752314","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8653693","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.428600013256073,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W1501988955","https://openalex.org/W1537211771","https://openalex.org/W1573411722","https://openalex.org/W1971529761","https://openalex.org/W1979390430","https://openalex.org/W1980480613","https://openalex.org/W2026151657","https://openalex.org/W2044754306","https://openalex.org/W2048172586","https://openalex.org/W2049220062","https://openalex.org/W2052626837","https://openalex.org/W2141959092","https://openalex.org/W2167709602"],"related_works":["https://openalex.org/W2345849370","https://openalex.org/W2063560538","https://openalex.org/W2019551517","https://openalex.org/W2068843202","https://openalex.org/W2055360422","https://openalex.org/W2025257746","https://openalex.org/W2132012838","https://openalex.org/W2078567932","https://openalex.org/W2016054018","https://openalex.org/W2144763197"],"abstract_inverted_index":{"Membrane-type":[0],"1":[1,75],"matrix":[2,76],"metalloproteinase":[3,77],"that":[4,42],"is":[5,78],"associated":[6],"with":[7],"the":[8,28],"proteolytic":[9],"activation":[10],"of":[11,31,46,62,71,82],"progelatinase":[12,40,83],"A":[13,33,38,41,84],"was":[14,50,57],"expressed":[15],"as":[16],"a":[17,35,44,79],"recombinant":[18,25],"fusion":[19],"protein":[20],"in":[21,34],"Escherichia":[22],"coli.":[23],"The":[24,55],"enzyme":[26],"cleaved":[27],"propeptide":[29],"sequence":[30],"gelatinase":[32],"sequence-specific":[36],"manner.":[37],"mutant":[39],"has":[43],"substitution":[45],"Asn(66)-Leu":[47],"to":[48],"Ile-Val":[49],"not":[51,67],"processed":[52],"at":[53],"all.":[54],"processing":[56],"blocked":[58],"by":[59,68],"tissue":[60,69],"inhibitor":[61,70],"metalloproteinases-2":[63],"or":[64],"BB-94":[65],"but":[66],"metalloproteinases-1.":[72],"Thus,":[73],"membrane-type":[74],"direct":[80],"activator":[81],"without":[85],"requiring":[86],"additional":[87],"proteases.":[88]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}