{"id":"https://openalex.org/W1546486342","doi":"https://doi.org/10.1038/sj.bjp.0701238","title":"Pharmacological characterization of type 1\u03b1 metabotropic glutamate receptor\u2010stimulated [35S]\u2010GTP\u03b3S binding","display_name":"Pharmacological characterization of type 1\u03b1 metabotropic glutamate receptor\u2010stimulated [35S]\u2010GTP\u03b3S binding","publication_year":1997,"publication_date":"1997-07-01","ids":{"openalex":"https://openalex.org/W1546486342","doi":"https://doi.org/10.1038/sj.bjp.0701238","mag":"1546486342","pmid":"https://pubmed.ncbi.nlm.nih.gov/9249258","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1564797"},"language":"en","primary_location":{"id":"doi:10.1038/sj.bjp.0701238","doi":"https://doi.org/10.1038/sj.bjp.0701238","is_oa":true,"landing_page_url":"https://doi.org/10.1038/sj.bjp.0701238","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1038/sj.bjp.0701238","source":{"id":"https://openalex.org/S55107261","display_name":"British Journal of Pharmacology","issn_l":"0007-1188","issn":["0007-1188","1476-5381"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"British Journal of Pharmacology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1038/sj.bjp.0701238","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082706655","display_name":"Elizabeth C. Akam","orcid":"https://orcid.org/0000-0002-8784-3742","observed_orcids":["https://orcid.org/0000-0002-8784-3742"]},"institutions":[{"id":"https://openalex.org/I153648349","display_name":"University of Leicester","ror":"https://ror.org/04h699437","country_code":"GB","type":"education","lineage":["https://openalex.org/I153648349"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Elizabeth C Akam","raw_affiliation_strings":["Department of Cell Physiology and Pharmacology, University of Leicester","Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Physiology and Pharmacology, University of Leicester","institution_ids":["https://openalex.org/I153648349"]},{"raw_affiliation_string":"Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN","institution_ids":["https://openalex.org/I153648349"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111648867","display_name":"Alan M. Carruthers","orcid":"https://orcid.org/0009-0008-2701-672X","observed_orcids":["https://orcid.org/0009-0008-2701-672X"]},"institutions":[{"id":"https://openalex.org/I153648349","display_name":"University of Leicester","ror":"https://ror.org/04h699437","country_code":"GB","type":"education","lineage":["https://openalex.org/I153648349"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Alan M Carruthers","raw_affiliation_strings":["Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN","institution_ids":["https://openalex.org/I153648349"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113984991","display_name":"Stefan R. Nahorski","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I153648349","display_name":"University of Leicester","ror":"https://ror.org/04h699437","country_code":"GB","type":"education","lineage":["https://openalex.org/I153648349"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Stefan R Nahorski","raw_affiliation_strings":["Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN","institution_ids":["https://openalex.org/I153648349"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5044018474","display_name":"R. A. John Challiss","orcid":"https://orcid.org/0000-0001-5506-2848","observed_orcids":["https://orcid.org/0000-0001-5506-2848"]},"institutions":[{"id":"https://openalex.org/I153648349","display_name":"University of Leicester","ror":"https://ror.org/04h699437","country_code":"GB","type":"education","lineage":["https://openalex.org/I153648349"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"R A John Challiss","raw_affiliation_strings":["Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Physiology and Pharmacology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Road, Leicester, LE1 9HN","institution_ids":["https://openalex.org/I153648349"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5082706655"],"corresponding_institution_ids":["https://openalex.org/I153648349"],"apc_list":{"value":4780,"currency":"USD","value_usd":4780},"apc_paid":null,"fwci":1.6868,"has_fulltext":true,"cited_by_count":40,"citation_normalized_percentile":{"value":0.83773479,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"121","issue":"6","first_page":"1203","last_page":"1209"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.49900001287460327,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.49900001287460327,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.49390000104904175,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/metabotropic-glutamate-receptor-1","display_name":"metabotropic glutamate receptor 1","score":0.7580000162124634},{"id":"https://openalex.org/keywords/mglur1alpha","display_name":"mGluR1alpha","score":0.9129999876022339},{"id":"https://openalex.org/keywords/g-protein-activation","display_name":"G protein activation","score":0.9409999847412109},{"id":"https://openalex.org/keywords/glutamate","display_name":"glutamate","score":0.7170000076293945},{"id":"https://openalex.org/keywords/quisqualate","display_name":"quisqualate","score":0.7929999828338623},{"id":"https://openalex.org/keywords/1s-3r-acpd","display_name":"1S,3R-ACPD","score":0.8220000267028809},{"id":"https://openalex.org/keywords/partial-agonists","display_name":"partial agonists","score":0.7110000252723694},{"id":"https://openalex.org/keywords/gtpgammas-binding","display_name":"GTPgammaS binding","score":0.621999979019165},{"id":"https://openalex.org/keywords/receptor-pharmacology","display_name":"receptor pharmacology","score":0.31299999356269836}],"concepts":[{"id":"https://openalex.org/C207951395","wikidata":"https://www.wikidata.org/wiki/Q288431","display_name":"Metabotropic glutamate receptor 1","level":5,"score":0.8612000942230225},{"id":"https://openalex.org/C118716","wikidata":"https://www.wikidata.org/wiki/Q5514725","display_name":"GTP'","level":3,"score":0.7779384255409241},{"id":"https://openalex.org/C49051014","wikidata":"https://www.wikidata.org/wiki/Q2033959","display_name":"Metabotropic glutamate receptor","level":4,"score":0.7548314929008484},{"id":"https://openalex.org/C41653306","wikidata":"https://www.wikidata.org/wiki/Q6589190","display_name":"Metabotropic glutamate receptor 5","level":5,"score":0.61948162317276},{"id":"https://openalex.org/C26702167","wikidata":"https://www.wikidata.org/wiki/Q901988","display_name":"Metabotropic receptor","level":4,"score":0.5885709524154663},{"id":"https://openalex.org/C56940625","wikidata":"https://www.wikidata.org/wiki/Q6589868","display_name":"Metabotropic glutamate receptor 6","level":5,"score":0.5414555072784424},{"id":"https://openalex.org/C61174792","wikidata":"https://www.wikidata.org/wiki/Q420510","display_name":"Glutamate receptor","level":3,"score":0.5214766263961792},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5001533031463623},{"id":"https://openalex.org/C119128870","wikidata":"https://www.wikidata.org/wiki/Q4859538","display_name":"Metabotropic glutamate receptor 2","level":5,"score":0.4996297359466553},{"id":"https://openalex.org/C87247309","wikidata":"https://www.wikidata.org/wiki/Q6590338","display_name":"Metabotropic glutamate receptor 7","level":5,"score":0.4960730969905853},{"id":"https://openalex.org/C80631254","wikidata":"https://www.wikidata.org/wiki/Q407438","display_name":"G protein","level":3,"score":0.49416521191596985},{"id":"https://openalex.org/C2777995097","wikidata":"https://www.wikidata.org/wiki/Q422462","display_name":"Guanosine","level":2,"score":0.4162661135196686},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38975316286087036},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.37721800804138184},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3466702401638031},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.340045690536499},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3069896697998047},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010566","descriptor_name":"Virulence Factors, Bordetella","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011869","descriptor_name":"Radioligand Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013462","descriptor_name":"Sulfur Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016244","descriptor_name":"Guanosine 5'-O-(3-Thiotriphosphate)","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018094","descriptor_name":"Receptors, Metabotropic Glutamate","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D018094","descriptor_name":"Receptors, Metabotropic Glutamate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018690","descriptor_name":"Excitatory Amino Acid Agonists","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D019204","descriptor_name":"GTP-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D037342","descriptor_name":"Pertussis Toxin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1038/sj.bjp.0701238","doi":"https://doi.org/10.1038/sj.bjp.0701238","is_oa":true,"landing_page_url":"https://doi.org/10.1038/sj.bjp.0701238","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1038/sj.bjp.0701238","source":{"id":"https://openalex.org/S55107261","display_name":"British Journal of Pharmacology","issn_l":"0007-1188","issn":["0007-1188","1476-5381"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"British Journal of Pharmacology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3469658","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1564797","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:1564797","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1564797","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Br J Pharmacol","raw_type":"Text"},{"id":"pmid:9249258","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9249258","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"British journal of pharmacology","raw_type":"Journal Article"},{"id":"pmh:oai:lra.le.ac.uk:2381/25080","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/2381/25080","pdf_url":null,"source":{"id":"https://openalex.org/S4306402365","display_name":"Leicester Research Archive (University of Leicester)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I153648349","host_organization_name":"University of Leicester","host_organization_lineage":["https://openalex.org/I153648349"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scopus","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1038/sj.bjp.0701238","doi":"https://doi.org/10.1038/sj.bjp.0701238","is_oa":true,"landing_page_url":"https://doi.org/10.1038/sj.bjp.0701238","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1038/sj.bjp.0701238","source":{"id":"https://openalex.org/S55107261","display_name":"British Journal of Pharmacology","issn_l":"0007-1188","issn":["0007-1188","1476-5381"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"British Journal of Pharmacology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320311904","display_name":"Wellcome Trust","ror":"https://ror.org/029chgv08"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1546486342.pdf","grobid_xml":"https://content.openalex.org/works/W1546486342.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W1484188159","https://openalex.org/W1497141468","https://openalex.org/W1506425398","https://openalex.org/W1509785349","https://openalex.org/W1893612865","https://openalex.org/W1927872096","https://openalex.org/W1950417202","https://openalex.org/W1969762570","https://openalex.org/W1971583983","https://openalex.org/W1979035442","https://openalex.org/W1982340263","https://openalex.org/W1991542726","https://openalex.org/W1996405522","https://openalex.org/W2003105549","https://openalex.org/W2003760589","https://openalex.org/W2005069084","https://openalex.org/W2005913521","https://openalex.org/W2008158760","https://openalex.org/W2014472421","https://openalex.org/W2043166209","https://openalex.org/W2050419480","https://openalex.org/W2052284394","https://openalex.org/W2053153052","https://openalex.org/W2056403651","https://openalex.org/W2058777019","https://openalex.org/W2062455944","https://openalex.org/W2066439059","https://openalex.org/W2073473779","https://openalex.org/W2078935283","https://openalex.org/W2080875376","https://openalex.org/W2095388820","https://openalex.org/W2161584310","https://openalex.org/W2344024966","https://openalex.org/W2399928535","https://openalex.org/W2403466950","https://openalex.org/W2407549567","https://openalex.org/W2414158244"],"related_works":["https://openalex.org/W1845103358","https://openalex.org/W2087508161","https://openalex.org/W2076218499","https://openalex.org/W2002877168","https://openalex.org/W2049587891","https://openalex.org/W2040048088","https://openalex.org/W2165065027","https://openalex.org/W1810539664","https://openalex.org/W1981992372","https://openalex.org/W1982340263"],"abstract_inverted_index":{"1.":[0],"The":[1,95,294],"activation":[2],"of":[3,32,44,109,121,126,135,146,155,160,213,228,242,248,296],"G":[4,127,157,322],"proteins":[5,128,158],"by":[6,30,130,287,299],"type":[7],"1alpha":[8],"metabotropic":[9],"glutamate":[10,169],"receptors":[11],"(mGluRs)":[12],"in":[13,47,100,106,191,266,270,303,312],"membranes":[14,304],"from":[15,305],"recombinant":[16,306],"baby":[17],"hamster":[18],"kidney":[19],"cells":[20,244,307],"expressing":[21],"the":[22,42,107,110,122,196,214,288],"cloned":[23],"rat":[24],"mGluR1alpha":[25],"receptor":[26,170],"has":[27],"been":[28],"studied":[29],"use":[31],"a":[33,48,131,152,192,209,246,267],"[35S]-guanosine":[34],"5'-[gamma-thio]triphosphate":[35],"([35S]-GTPgammaS)":[36],"binding":[37,46,65,102,145,190,273,286,298],"assay.":[38],"2.":[39],"L-Glutamate":[40],"increased":[41,188],"rate":[43],"[35S]-GTPgammaS":[45,101,144,189,219,272,285,297],"concentration-dependent":[49,193],"manner":[50],"(-logEC50":[51,173],"(M)":[52,174],"5.25":[53],"+/-":[54,60,148,162,176,180,185,230,276],"0.07),":[55],"with":[56,76,195,245,318],"an":[57,138,222,279,309],"optimal":[58],"(62.4":[59],"1.6%)":[61],"increase":[62,99],"over":[63],"basal":[64],"being":[66],"observed":[67],"following":[68],"60":[69],"min":[70],"incubation":[71],"at":[72],"30":[73,163],"degrees":[74],"C":[75],"70":[77],"pM":[78],"[35S]-GTPgammaS,":[79],"1":[80],"microM":[81,232],"GDP,":[82],"10":[83],"mM":[84,87],"MgCl2,":[85],"100":[86,90],"NaCl":[88],"and":[89,124,151,182,278,301,320],"microg":[91],"membrane":[92,263],"protein":[93],"ml(-1).":[94],"L-glutamate":[96,136,215],"(100":[97,257],"microM)-stimulated":[98],"was":[103],"totally":[104],"prevented":[105],"presence":[108],"group":[111],"I":[112],"mGluR":[113,238,316],"antagonist":[114,223],"(S)-4-carboxy-3-hydroxyphenylglycine":[115],"(300":[116,206],"microM).":[117],"3.":[118],"Quantitative":[119],"analysis":[120],"affinity":[123],"number":[125,154],"activated":[129],"maximally":[132,255],"effective":[133,256],"concentration":[134,247],"revealed":[137],"equilibrium":[139,224],"dissociation":[140,225],"constant":[141,226],"(K(D))":[142,227],"for":[143,218,236,283],"0.76":[147],"0.20":[149],"nM":[150],"maximal":[153],"GTPgammaS-liganded":[156],"(Bmax)":[159],"361":[161],"fmol":[164],"mg(-1)":[165],"protein.":[166],"4.":[167],"Metabotropic":[168],"agonists,":[171],"quisqualate":[172],"6.74":[175],"0.06),":[177],"1S,3R-ACPD":[178],"(4.64":[179],"0.08)":[181],"(S)-3,5-dihydroxyphenylglycine":[183],"(5.16":[184],"0.23)":[186],"also":[187],"manner,":[194],"latter":[197],"two":[198],"agents":[199],"behaving":[200],"as":[201],"partial":[202],"agonists.":[203],"5.":[204],"(+)-alpha-Methylcarboxyphenylglycine":[205],"microM)":[207],"caused":[208],"parallel":[210],"rightward":[211],"shift":[212],"concentration-effect":[216,281],"curve":[217],"binding,":[220],"allowing":[221],"34.0":[229],"7.8":[231],"to":[233,253,314],"be":[234,254],"calculated":[235],"this":[237],"antagonist.":[239],"6.":[240],"Pretreatment":[241],"BHK-mGluR1alpha":[243],"pertussis":[249],"toxin":[250],"(PTX)":[251],"shown":[252],"ng":[258],"ml(-1),":[259],"24":[260],"h)":[261],"before":[262],"preparation":[264],"resulted":[265],"marked":[268],"decrease":[269],"agonist-stimulated":[271,284],"(by":[274],"66.0":[275],"0.9%),":[277],"altered":[280],"relationship":[282],"residual":[289],"PTX-insensitive":[290],"G-protein":[291],"population.":[292],"7.":[293],"modulation":[295],"agonists":[300],"antagonists":[302],"provides":[308],"excellent":[310],"system":[311],"which":[313],"study":[315],"interactions":[317],"PTX-sensitive":[319],"-insensitive":[321],"proteins.":[323]},"counts_by_year":[{"year":2021,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}