{"id":"https://openalex.org/W1542077904","doi":"https://doi.org/10.1128/jb.173.2.768-775.1991","title":"Conservation of the gene for outer membrane protein OprF in the family Pseudomonadaceae: sequence of the Pseudomonas syringae oprF gene","display_name":"Conservation of the gene for outer membrane protein OprF in the family Pseudomonadaceae: sequence of the Pseudomonas syringae oprF gene","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W1542077904","doi":"https://doi.org/10.1128/jb.173.2.768-775.1991","mag":"1542077904","pmid":"https://pubmed.ncbi.nlm.nih.gov/1898935","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/207070"},"language":"en","primary_location":{"id":"doi:10.1128/jb.173.2.768-775.1991","doi":"https://doi.org/10.1128/jb.173.2.768-775.1991","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.173.2.768-775.1991","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC207070","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004857233","display_name":"Catherine Ann MacDonald Ullstrom","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"C A Ullstrom","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015244359","display_name":"Richard Siehnel","orcid":"https://orcid.org/0000-0001-7078-5657","observed_orcids":["https://orcid.org/0000-0001-7078-5657"]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"R Siehnel","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091657501","display_name":"W A Woodruff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"W Woodruff","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026846286","display_name":"Suzanne Steinbach","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"S Steinbach","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5066530115","display_name":"Robert E. W. Hancock","orcid":"https://orcid.org/0000-0001-5989-8503","observed_orcids":["https://orcid.org/0000-0001-5989-8503","https://orcid.org/0000-0003-2231-4716","https://orcid.org/0000-0001-5465-3814"]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"R E Hancock","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I141945490"],"apc_list":null,"apc_paid":null,"fwci":2.7771,"has_fulltext":false,"cited_by_count":35,"citation_normalized_percentile":{"value":0.89590592,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"173","issue":"2","first_page":"768","last_page":"775"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.5479000210762024,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.5479000210762024,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11578","display_name":"Plant Pathogenic Bacteria Studies","score":0.43380001187324524,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.002899999963119626,"subfield":{"id":"https://openalex.org/subfields/1313","display_name":"Molecular Medicine"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oprf","display_name":"OprF","score":0.9120000004768372},{"id":"https://openalex.org/keywords/outer-membrane-proteins","display_name":"outer membrane proteins","score":0.8069999814033508},{"id":"https://openalex.org/keywords/pseudomonas-syringae","display_name":"Pseudomonas syringae","score":0.9459999799728394},{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":0.9990000128746033},{"id":"https://openalex.org/keywords/pseudomonadaceae","display_name":"Pseudomonadaceae","score":0.9860000014305115},{"id":"https://openalex.org/keywords/genetic-conservation","display_name":"genetic conservation","score":0.902999997138977},{"id":"https://openalex.org/keywords/restriction-mapping","display_name":"restriction mapping","score":0.5720000267028809},{"id":"https://openalex.org/keywords/ompa","display_name":"OmpA","score":0.5099999904632568}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8538053035736084},{"id":"https://openalex.org/C2780381237","wikidata":"https://www.wikidata.org/wiki/Q311202","display_name":"Pseudomonas syringae","level":3,"score":0.7335088849067688},{"id":"https://openalex.org/C165525559","wikidata":"https://www.wikidata.org/wiki/Q224180","display_name":"Homology (biology)","level":3,"score":0.5455020666122437},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5160284042358398},{"id":"https://openalex.org/C2776738503","wikidata":"https://www.wikidata.org/wiki/Q138058","display_name":"Pseudomonadaceae","level":4,"score":0.5055615901947021},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5020833015441895},{"id":"https://openalex.org/C133307689","wikidata":"https://www.wikidata.org/wiki/Q48749","display_name":"Southern blot","level":3,"score":0.4878425598144531},{"id":"https://openalex.org/C170673058","wikidata":"https://www.wikidata.org/wiki/Q780686","display_name":"Restriction map","level":4,"score":0.45364782214164734},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.44122716784477234},{"id":"https://openalex.org/C168007829","wikidata":"https://www.wikidata.org/wiki/Q857299","display_name":"Restriction fragment length polymorphism","level":4,"score":0.4237549304962158},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.39698466658592224},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.3955073654651642},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.34660202264785767},{"id":"https://openalex.org/C2781463711","wikidata":"https://www.wikidata.org/wiki/Q131143","display_name":"Pseudomonas","level":3,"score":0.29979920387268066},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.1826474964618683},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.12297973036766052}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008051","descriptor_name":"Lipid Bilayers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011548","descriptor_name":"Pseudomonadaceae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011550","descriptor_name":"Pseudomonas aeruginosa","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012689","descriptor_name":"Sequence Homology, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018272","descriptor_name":"Porins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/jb.173.2.768-775.1991","doi":"https://doi.org/10.1128/jb.173.2.768-775.1991","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.173.2.768-775.1991","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:79642","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC207070","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:207070","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/207070","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Bacteriol","raw_type":"Text"},{"id":"pmid:1898935","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1898935","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bacteriology","raw_type":"Comparative Study"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:79642","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC207070","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.7599999904632568,"id":"https://metadata.un.org/sdg/15","display_name":"Life on land"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W84470803","https://openalex.org/W176353646","https://openalex.org/W1492085418","https://openalex.org/W1495487659","https://openalex.org/W1497877769","https://openalex.org/W1537452440","https://openalex.org/W1568947256","https://openalex.org/W1584305148","https://openalex.org/W1606263729","https://openalex.org/W1667881014","https://openalex.org/W1891960973","https://openalex.org/W1965332298","https://openalex.org/W1965546960","https://openalex.org/W1967999707","https://openalex.org/W1973383784","https://openalex.org/W1996821881","https://openalex.org/W1999187539","https://openalex.org/W2001426244","https://openalex.org/W2009918551","https://openalex.org/W2032462421","https://openalex.org/W2050524698","https://openalex.org/W2054179773","https://openalex.org/W2074231493","https://openalex.org/W2085005062","https://openalex.org/W2094788542","https://openalex.org/W2096276876","https://openalex.org/W2099569409","https://openalex.org/W2103425504","https://openalex.org/W2116909622","https://openalex.org/W2129017226","https://openalex.org/W2129234112","https://openalex.org/W2137847721","https://openalex.org/W2138270253","https://openalex.org/W2144634347","https://openalex.org/W2146081652","https://openalex.org/W2155719288","https://openalex.org/W2171074162","https://openalex.org/W2896593941"],"related_works":["https://openalex.org/W7097989532","https://openalex.org/W7095420974","https://openalex.org/W2289386517","https://openalex.org/W2104551117","https://openalex.org/W2077455931","https://openalex.org/W2143128511","https://openalex.org/W2461349082","https://openalex.org/W2011018119","https://openalex.org/W4319291058","https://openalex.org/W2063891245"],"abstract_inverted_index":{"The":[0,31,173,194,240],"conservation":[1],"of":[2,39,48,68,72,85,100,115,153,177,209,244,258],"the":[3,7,23,40,52,76,82,92,97,102,124,146,159,178,206,219,226,255,259],"oprF":[4,26,93,103,195,247,262],"gene":[5,27,104,127,196,221],"for":[6],"major":[8],"outer":[9,143],"membrane":[10],"protein":[11,140,160,176],"OprF":[12,139,175,220],"was":[13,34,167,201,222],"determined":[14],"by":[15],"restriction":[16,32,64,134],"mapping":[17],"and":[18,44,56,105,166,170,203,215,233,250,263],"Southern":[19,66],"blot":[20],"hybridization":[21,211],"with":[22,91,123,145,162,228,254],"Pseudomonas":[24,111],"aeruginosa":[25,126,214,261],"as":[28,151],"a":[29],"probe.":[30],"map":[33],"highly":[35,223],"conserved":[36,224],"among":[37],"16":[38],"17":[41],"serotype":[42,53],"strains":[43,114],"42":[45],"clinical":[46,58],"isolates":[47],"P.":[49,116,125,181,213,216,245,260],"aeruginosa.":[50],"Only":[51],"12":[54],"isolate":[55,59],"one":[57],"showed":[60,248],"small":[61,188],"differences":[62],"in":[63,110,141,190],"pattern.":[65],"probing":[67],"PstI":[69],"chromosomal":[70],"digests":[71],"14":[73],"species":[74,227],"from":[75,118,197],"family":[77],"Pseudomonadaceae":[78],"revealed":[79],"that":[80,152],"only":[81],"nine":[83],"members":[84],"rRNA":[86],"homology":[87],"group":[88],"I":[89],"hybridized":[90,122],"gene.":[94],"To":[95],"reveal":[96],"actual":[98],"extent":[99],"homology,":[101],"its":[106],"product":[107],"were":[108],"characterized":[109],"syringae.":[112],"Nine":[113],"syringae":[117,182,184,217,246],"seven":[119],"different":[120,131],"pathovars":[121],"to":[128],"produce":[129],"five":[130],"but":[132],"related":[133],"maps.":[135],"All":[136],"produced":[137],"an":[138],"their":[142],"membranes":[144],"same":[147,256],"apparent":[148],"molecular":[149],"weight":[150],"P.aeruginosa":[154],"OprF.":[155],"In":[156],"each":[157],"case":[158],"reacted":[161],"monoclonal":[163],"antibody":[164],"MA4-10":[165],"similarly":[168],"heat":[169],"2-mercaptoethanol":[171],"modifiable.":[172],"purified":[174],"type":[179],"strain":[180,200],"pv.":[183],"ATCC":[185],"19310":[186],"reconstituted":[187],"channels":[189],"lipid":[191],"bilayer":[192],"membranes.":[193],"this":[198],"latter":[199],"cloned":[202],"sequenced.":[204],"Despite":[205],"low":[207],"level":[208],"DNA":[210,230],"between":[212,225],"DNA,":[218],"72%":[229],"sequence":[231,237],"identity":[232,238],"68%":[234],"amino":[235],"acid":[236],"overall.":[239],"carboxy":[241],"terminus-encoding":[242],"region":[243],"85":[249],"33%":[251],"identity,":[252],"respectively,":[253],"regions":[257],"Escherichia":[264],"coli":[265],"ompA":[266],"genes.":[267]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}