{"id":"https://openalex.org/W1541581001","doi":"https://doi.org/10.1042/bj2570809","title":"The purification of a 50 kDa protein-actin complex from unfertilized sea-urchin (Strongylocentrotus purpuratus) eggs","display_name":"The purification of a 50 kDa protein-actin complex from unfertilized sea-urchin (Strongylocentrotus purpuratus) eggs","publication_year":1989,"publication_date":"1989-02-01","ids":{"openalex":"https://openalex.org/W1541581001","doi":"https://doi.org/10.1042/bj2570809","mag":"1541581001","pmid":"https://pubmed.ncbi.nlm.nih.gov/2930488","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1135660"},"language":"en","primary_location":{"id":"doi:10.1042/bj2570809","doi":"https://doi.org/10.1042/bj2570809","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2570809","pdf_url":"https://portlandpress.com/biochemj/article-pdf/257/3/809/595570/bj2570809.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://portlandpress.com/biochemj/article-pdf/257/3/809/595570/bj2570809.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019568988","display_name":"Roy M. Golsteyn","orcid":"https://orcid.org/0000-0002-0251-7025","observed_orcids":["https://orcid.org/0000-0002-0251-7025"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"R M Golsteyn","raw_affiliation_strings":["Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada, T2N 4N1"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada, T2N 4N1","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091852858","display_name":"David M. Waisman","orcid":"https://orcid.org/0000-0002-5097-9662","observed_orcids":["https://orcid.org/0000-0002-5097-9662"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"D M Waisman","raw_affiliation_strings":["Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada, T2N 4N1"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada, T2N 4N1","institution_ids":["https://openalex.org/I168635309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I168635309"],"apc_list":null,"apc_paid":null,"fwci":0.1289,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":{"value":0.3777022,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"257","issue":"3","first_page":"809","last_page":"815"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.10809999704360962,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.10809999704360962,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.07370000332593918,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.07029999792575836,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/strongylocentrotus-purpuratus","display_name":"Strongylocentrotus purpuratus","score":0.9490000009536743},{"id":"https://openalex.org/keywords/calcium-binding-proteins","display_name":"calcium-binding proteins","score":0.8069999814033508},{"id":"https://openalex.org/keywords/actin","display_name":"actin","score":0.8700000047683716},{"id":"https://openalex.org/keywords/protein-complex-purification","display_name":"protein complex purification","score":0.625},{"id":"https://openalex.org/keywords/deae-cellulose-fractionation","display_name":"DEAE-cellulose fractionation","score":0.8040000200271606},{"id":"https://openalex.org/keywords/calcium-binding-activity","display_name":"calcium-binding activity","score":0.4390000104904175},{"id":"https://openalex.org/keywords/protein-complex-stability","display_name":"protein complex stability","score":0.36399999260902405}],"concepts":[{"id":"https://openalex.org/C2777569064","wikidata":"https://www.wikidata.org/wiki/Q1936405","display_name":"Strongylocentrotus purpuratus","level":3,"score":0.9671652317047119},{"id":"https://openalex.org/C2776908312","wikidata":"https://www.wikidata.org/wiki/Q83483","display_name":"Sea urchin","level":2,"score":0.8903795480728149},{"id":"https://openalex.org/C2777658833","wikidata":"https://www.wikidata.org/wiki/Q408437","display_name":"EGTA","level":3,"score":0.8433021903038025},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6420577764511108},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.5824928879737854},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.5139512419700623},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5040677785873413},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.45934802293777466},{"id":"https://openalex.org/C553089730","wikidata":"https://www.wikidata.org/wiki/Q4914021","display_name":"Binding protein","level":3,"score":0.44753503799438477},{"id":"https://openalex.org/C122229402","wikidata":"https://www.wikidata.org/wiki/Q5018802","display_name":"Calcium-binding protein","level":3,"score":0.44432058930397034},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.42558449506759644},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36599159240722656},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.32955795526504517},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.2683626413345337},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2385997474193573},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.07290777564048767},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004527","descriptor_name":"Egg Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004527","descriptor_name":"Egg Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005306","descriptor_name":"Fertilization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010063","descriptor_name":"Ovum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012617","descriptor_name":"Sea Urchins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1042/bj2570809","doi":"https://doi.org/10.1042/bj2570809","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2570809","pdf_url":"https://portlandpress.com/biochemj/article-pdf/257/3/809/595570/bj2570809.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1135660","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1135660","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmid:2930488","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2930488","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1042/bj2570809","doi":"https://doi.org/10.1042/bj2570809","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2570809","pdf_url":"https://portlandpress.com/biochemj/article-pdf/257/3/809/595570/bj2570809.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1541581001.pdf","grobid_xml":"https://content.openalex.org/works/W1541581001.grobid-xml"},"referenced_works_count":41,"referenced_works":["https://openalex.org/W654843005","https://openalex.org/W1541586041","https://openalex.org/W1554038443","https://openalex.org/W1574128379","https://openalex.org/W1580184559","https://openalex.org/W1591082289","https://openalex.org/W1765359398","https://openalex.org/W1948035881","https://openalex.org/W1960390165","https://openalex.org/W1966532142","https://openalex.org/W1976868716","https://openalex.org/W1980148057","https://openalex.org/W1989251445","https://openalex.org/W1991465262","https://openalex.org/W1993616255","https://openalex.org/W2003579973","https://openalex.org/W2004993580","https://openalex.org/W2012174886","https://openalex.org/W2018289835","https://openalex.org/W2046060747","https://openalex.org/W2049312991","https://openalex.org/W2058442820","https://openalex.org/W2067119870","https://openalex.org/W2069386024","https://openalex.org/W2077080379","https://openalex.org/W2079337500","https://openalex.org/W2081243527","https://openalex.org/W2086462055","https://openalex.org/W2099548366","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2110565348","https://openalex.org/W2128635872","https://openalex.org/W2140807560","https://openalex.org/W2150152732","https://openalex.org/W2151007392","https://openalex.org/W6600001191","https://openalex.org/W6600231155","https://openalex.org/W6633640434","https://openalex.org/W6637305119","https://openalex.org/W6685849262"],"related_works":["https://openalex.org/W1591082289","https://openalex.org/W2063121205","https://openalex.org/W2563703845","https://openalex.org/W2791529645","https://openalex.org/W1960390165","https://openalex.org/W2025456088","https://openalex.org/W1909117184","https://openalex.org/W1874766351","https://openalex.org/W1948035881","https://openalex.org/W1506316133"],"abstract_inverted_index":{"The":[0,31,95,103,115],"100,000":[1],"g":[2],"supernatant":[3],"from":[4],"the":[5,9,25,43,72,78],"unfertilized":[6],"egg":[7],"of":[8,34,68,83,88,90],"sea":[10],"urchin":[11],"Strongylocentrotus":[12],"purpuratus":[13],"has":[14,51],"been":[15,52],"fractionated":[16],"on":[17],"DEAE-cellulose":[18],"and":[19,42,54,92],"analysed":[20],"for":[21,47,71],"Ca2+-binding":[22,28,35,44,73],"activity":[23,29,36],"by":[24,61,108],"Chelex-100":[26],"competitive":[27],"assay.":[30],"major":[32],"peak":[33,50],"was":[37,99,105,118],"subjected":[38],"to":[39],"further":[40],"purification":[41],"protein":[45,81,98],"responsible":[46],"this":[48],"Ca2+-binding-activity":[49],"isolated":[53],"characterized.":[55],"Non-denaturing":[56],"polyacrylamide-gel":[57],"electrophoresis":[58],"(PAGE)":[59],"followed":[60],"45Ca2+":[62],"autoradiography":[63],"suggested":[64],"a":[65,84],"molecular":[66],"mass":[67],"80":[69,79],"kDa":[70,80,97],"protein.":[74],"SDS/PAGE":[75],"revealed":[76],"that":[77],"consisted":[82],"1:1":[85],"molar":[86],"complex":[87,104,117],"proteins":[89],"50":[91],"42":[93,96],"kDa.":[94],"identified":[100],"as":[101],"actin.":[102],"not":[106],"dissociated":[107],"extensive":[109],"dialysis":[110],"against":[111],"an":[112],"EGTA-containing":[113],"buffer.":[114],"EGTA-stable":[116],"named":[119],"'50K-A'.":[120]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}