{"id":"https://openalex.org/W1541538097","doi":null,"title":"Comparison of central and peripheral human corneal epithelium in tissue culture.","display_name":"Comparison of central and peripheral human corneal epithelium in tissue culture.","publication_year":1987,"publication_date":"1987-09-01","ids":{"openalex":"https://openalex.org/W1541538097","mag":"1541538097","pmid":"https://pubmed.ncbi.nlm.nih.gov/3170124"},"language":"en","primary_location":{"id":"pmid:3170124","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3170124","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043737176","display_name":"B Ebato","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"B Ebato","raw_affiliation_strings":["Department of Ophthalmology, Eye and Ear Institute of Pittsburgh, Pennsylvania 15213"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ophthalmology, Eye and Ear Institute of Pittsburgh, Pennsylvania 15213","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111877664","display_name":"Judith Friend","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Friend","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5063220824","display_name":"Richard A. Thoft","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R A Thoft","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":15.4251,"has_fulltext":false,"cited_by_count":281,"citation_normalized_percentile":{"value":0.99565551,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"28","issue":"10","first_page":"1450","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11828","display_name":"Corneal Surgery and Treatments","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11828","display_name":"Corneal Surgery and Treatments","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10563","display_name":"Corneal surgery and disorders","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10997","display_name":"Ocular Surface and Contact Lens","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/corneal-epithelium","display_name":"corneal epithelium","score":0.949999988079071},{"id":"https://openalex.org/keywords/corneal-explants","display_name":"corneal explants","score":0.7369999885559082},{"id":"https://openalex.org/keywords/central-cornea","display_name":"central cornea","score":0.7239999771118164},{"id":"https://openalex.org/keywords/peripheral-cornea","display_name":"peripheral cornea","score":0.9929999709129333},{"id":"https://openalex.org/keywords/corneal-stroma","display_name":"corneal stroma","score":0.6520000100135803},{"id":"https://openalex.org/keywords/corneal-tissue-culture","display_name":"corneal tissue culture","score":0.6140000224113464}],"concepts":[{"id":"https://openalex.org/C19374876","wikidata":"https://www.wikidata.org/wiki/Q247170","display_name":"Hemocytometer","level":2,"score":0.9429905414581299},{"id":"https://openalex.org/C2779257441","wikidata":"https://www.wikidata.org/wiki/Q2040445","display_name":"Corneal epithelium","level":3,"score":0.7822363376617432},{"id":"https://openalex.org/C529295009","wikidata":"https://www.wikidata.org/wiki/Q41301","display_name":"Epithelium","level":2,"score":0.7604606747627258},{"id":"https://openalex.org/C2776882836","wikidata":"https://www.wikidata.org/wiki/Q168291","display_name":"Cornea","level":2,"score":0.6463267803192139},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.49724486470222473},{"id":"https://openalex.org/C46762472","wikidata":"https://www.wikidata.org/wiki/Q178648","display_name":"Peripheral","level":2,"score":0.48918870091438293},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.46156778931617737},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44766733050346375},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3496789038181305},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2802470922470093},{"id":"https://openalex.org/C118487528","wikidata":"https://www.wikidata.org/wiki/Q161437","display_name":"Ophthalmology","level":1,"score":0.2607981562614441},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.25366902351379395},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.08791854977607727},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003315","descriptor_name":"Cornea","qualifier_ui":"Q000033","qualifier_name":"anatomy & histology","is_major_topic":false},{"descriptor_ui":"D003315","descriptor_name":"Cornea","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004848","descriptor_name":"Epithelium","qualifier_ui":"Q000033","qualifier_name":"anatomy & histology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008938","descriptor_name":"Mitosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:3170124","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3170124","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},{"id":"pmid:3623831","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3623831","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Comparative Study"},{"id":"mag:1541538097","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3170124","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6240000128746033,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1992858100","https://openalex.org/W2001780867","https://openalex.org/W2320414701","https://openalex.org/W2049221236","https://openalex.org/W56954153","https://openalex.org/W1986366467","https://openalex.org/W6990384137","https://openalex.org/W2073778886","https://openalex.org/W120806495","https://openalex.org/W22986999"],"abstract_inverted_index":{"Past":[0],"attempts":[1],"to":[2,19],"grow":[3,28],"human":[4,25,52,219,229],"corneal":[5,26,220,230],"epithelium":[6,27,146,154,200,221],"in":[7,30,54,59,205,224],"culture":[8,73,79,225],"had":[9,171,182],"limited":[10],"success,":[11],"with":[12,75],"confluence":[13,174,184],"rarely":[14],"attained.":[15],"This":[16],"work":[17],"is":[18],"determine":[20],"whether":[21],"different":[22],"areas":[23],"of":[24,42,46,96,117,127,142,144,152,177,187,192,199],"better":[29,223],"tissue":[31,78],"culture.":[32,55],"We":[33],"compared":[34],"the":[35,37,88,94,114,203,214],"extent,":[36],"mitotic":[38,140],"rates,":[39],"and":[40,44,50,93,175,185],"morphology":[41],"outgrowths":[43,97,119,129,143,170,181],"histology":[45],"explants":[47,99,193],"from":[48,62,98,108],"central":[49,118,145,169,206,228],"peripheral":[51,128,153,215,218],"corneas":[53],"Explants,":[56],"2":[57],"mm":[58],"diameter,":[60],"removed":[61],"eye":[63],"bank":[64],"eyes,":[65],"were":[66,90,211],"placed":[67],"epithelial":[68],"side":[69],"up":[70],"on":[71,202,213],"a":[72],"dish":[74],"modified":[76],"SHEM":[77],"medium":[80],"(Jumblatt":[81],"et":[82],"al,":[83],"1983).":[84],"After":[85,166],"7":[86],"days,":[87,168],"tissues":[89],"fixed,":[91],"stained":[92],"area":[95,116],"measured":[100],"using":[101],"an":[102],"image":[103],"processor.":[104],"For":[105],"eight":[106],"eyes":[107],"donors":[109],"averaging":[110],"66":[111],"yr":[112],"old,":[113],"average":[115],"was":[120,130,147],"7.8":[121],"+/-":[122,132,156,160],"1.1":[123],"mm2,":[124],"while":[125],"that":[126,151,195],"52.8":[131],"5.2":[133],"mm2":[134],"(P":[135,162],"less":[136,149,163],"than":[137,150,164,226],"0.001).":[138,165],"The":[139],"rate":[141],"significantly":[148],"(1.1":[155],"0.5%":[157],"vs":[158],"18.8":[159],"0.8%)":[161],"14":[167],"not":[172],"attained":[173,183],"consisted":[176,186],"large":[178],"cells.":[179,190],"Peripheral":[180],"small":[188],"polygonal":[189],"Histology":[191],"showed":[194],"only":[196],"one":[197],"layer":[198],"remained":[201],"stroma":[204],"explants,":[207],"but":[208],"several":[209],"layers":[210],"present":[212],"explants.":[216],"Thus,":[217],"grows":[222],"does":[227],"epithelium.":[231]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":12},{"year":2014,"cited_by_count":13},{"year":2013,"cited_by_count":12},{"year":2012,"cited_by_count":9}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}