{"id":"https://openalex.org/W1538201212","doi":"https://doi.org/10.6092/unina/fedoa/8376","title":"Synthetic heme-proteins in biosensors development","display_name":"Synthetic heme-proteins in biosensors development","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W1538201212","doi":"https://doi.org/10.6092/unina/fedoa/8376","mag":"1538201212"},"language":"en","primary_location":{"id":"doi:10.6092/unina/fedoa/8376","doi":"https://doi.org/10.6092/unina/fedoa/8376","is_oa":true,"landing_page_url":"https://doi.org/10.6092/unina/fedoa/8376","pdf_url":null,"source":{"id":"https://openalex.org/S7407050999","display_name":"Universit\u00e0 degli Studi di Napoli Federico II","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},"volume":null,"type":"article","indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.6092/unina/fedoa/8376","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066951281","display_name":"Paola Ringhieri","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ringhieri, Paola","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5066951281"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.10854597,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11434","display_name":"Electrochemical Analysis and Applications","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1603","display_name":"Electrochemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heme-proteins","display_name":"heme proteins","score":0.6819999814033508},{"id":"https://openalex.org/keywords/direct-electron-transfer","display_name":"direct electron transfer","score":0.8539999723434448},{"id":"https://openalex.org/keywords/electrochemical-biosensor","display_name":"electrochemical biosensor","score":0.8550000190734863},{"id":"https://openalex.org/keywords/deuteroporphyrin","display_name":"deuteroporphyrin","score":0.7390000224113464},{"id":"https://openalex.org/keywords/heme-redox-potential","display_name":"heme redox potential","score":0.8040000200271606},{"id":"https://openalex.org/keywords/fe-iii-coordination","display_name":"Fe(III) coordination","score":0.6179999709129333},{"id":"https://openalex.org/keywords/affinity-biosensors","display_name":"affinity biosensors","score":0.8040000200271606},{"id":"https://openalex.org/keywords/electrocatalysis","display_name":"electrocatalysis","score":0.4390000104904175},{"id":"https://openalex.org/keywords/electrochemical-dna-biosensor","display_name":"electrochemical DNA biosensor","score":0.32600000500679016},{"id":"https://openalex.org/keywords/electrochemical-immunoassay","display_name":"electrochemical immunoassay","score":0.5910000205039978},{"id":"https://openalex.org/keywords/electrochemical-dna-hybridization","display_name":"electrochemical DNA hybridization","score":0.35199999809265137}],"concepts":[{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.8934161067008972},{"id":"https://openalex.org/C10390740","wikidata":"https://www.wikidata.org/wiki/Q2282401","display_name":"Analyte","level":2,"score":0.6392078995704651},{"id":"https://openalex.org/C49853544","wikidata":"https://www.wikidata.org/wiki/Q206229","display_name":"Biomolecule","level":2,"score":0.6216409206390381},{"id":"https://openalex.org/C123669783","wikidata":"https://www.wikidata.org/wiki/Q902898","display_name":"Electron transfer","level":2,"score":0.5962609648704529},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5576030015945435},{"id":"https://openalex.org/C55904794","wikidata":"https://www.wikidata.org/wiki/Q82682","display_name":"Redox","level":2,"score":0.5193856358528137},{"id":"https://openalex.org/C139369640","wikidata":"https://www.wikidata.org/wiki/Q474536","display_name":"Amperometry","level":4,"score":0.4772081971168518},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.45908230543136597},{"id":"https://openalex.org/C17525397","wikidata":"https://www.wikidata.org/wiki/Q176140","display_name":"Electrode","level":2,"score":0.45558294653892517},{"id":"https://openalex.org/C2776217839","wikidata":"https://www.wikidata.org/wiki/Q189621","display_name":"Heme","level":3,"score":0.4219169616699219},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.42178255319595337},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.34430694580078125},{"id":"https://openalex.org/C52859227","wikidata":"https://www.wikidata.org/wiki/Q7877","display_name":"Electrochemistry","level":3,"score":0.3424375355243683},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.32861655950546265},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.19422227144241333},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14974543452262878},{"id":"https://openalex.org/C179104552","wikidata":"https://www.wikidata.org/wiki/Q11165","display_name":"Inorganic chemistry","level":1,"score":0.09863021969795227},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.0885089635848999},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.08190396428108215},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.6092/unina/fedoa/8376","doi":"https://doi.org/10.6092/unina/fedoa/8376","is_oa":true,"landing_page_url":"https://doi.org/10.6092/unina/fedoa/8376","pdf_url":null,"source":{"id":"https://openalex.org/S7407050999","display_name":"Universit\u00e0 degli Studi di Napoli Federico II","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},{"id":"mag:1538201212","doi":null,"is_oa":false,"landing_page_url":"http://www.fedoa.unina.it/8376/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.6092/unina/fedoa/8376","doi":"https://doi.org/10.6092/unina/fedoa/8376","is_oa":true,"landing_page_url":"https://doi.org/10.6092/unina/fedoa/8376","pdf_url":null,"source":{"id":"https://openalex.org/S7407050999","display_name":"Universit\u00e0 degli Studi di Napoli Federico II","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1979868790","https://openalex.org/W28081695","https://openalex.org/W3128763805","https://openalex.org/W2105748804","https://openalex.org/W3039322294","https://openalex.org/W1528859574","https://openalex.org/W7099153133","https://openalex.org/W1989773777","https://openalex.org/W1967141373","https://openalex.org/W2809299885"],"abstract_inverted_index":{"A":[0,85],"biosensor":[1,64,333],"is":[2,100,152,234],"an":[3,15,17,38,45,121,136],"analytical":[4,161],"device":[5,162],"that":[6,213],"relies":[7],"on":[8,148,204,209,243,388],"a":[9,19,25,30,51,127,182,254,389],"biological":[10,132],"recognition":[11,402],"element,":[12],"such":[13],"as":[14,341],"enzyme,":[16],"antibody,":[18],"DNA":[20],"fragment,":[21],"which":[22,43],"communicates":[23],"with":[24,94,126,166,224,265],"signal":[26,56],"transducer,":[27],"thus":[28],"providing":[29],"measurable":[31],"response":[32],"related":[33],"to":[34,78,220,229,246,306,353,393],"the":[35,55,59,79,89,103,110,131,140,153,157,160,225,231,235,248,266,270,273,281,292,296,302,307,314,322,327,362,375,395,400],"concentration":[36],"of":[37,68,70,91,120,130,139,145,155,159,185,190,237,257,268,272,288,301,316,320,349,365,374],"analyte.":[39,141],"Currently,":[40],"electrochemical":[41,92,372],"biosensors,":[42],"exploit":[44,109],"electron":[46,113,150,174],"transfer":[47,114,151,175],"(ET)":[48],"event":[49],"at":[50,116],"solid":[52],"electrode":[53,122,391],"for":[54,81,181,345,361],"transduction,":[57],"are":[58,217,291],"larger":[60],"and":[61,75,98,197,277,295,326,355,358,398],"most":[62],"promising":[63],"category":[65],"in":[66,82,135,279,332,368],"terms":[67],"ease":[69],"construction,":[71],"costs,":[72],"versatility,":[73],"tunability":[74],"miniaturization":[76],"(up":[77],"nanoscale)":[80],"vivo":[83],"applications.":[84],"real":[86],"breakthrough":[87],"toward":[88],"development":[90],"systems":[93,146],"superior":[95],"selectivity,":[96],"stability":[97],"durability":[99],"represented":[101],"by":[102,380],"third":[104],"generation":[105],"biosensors.":[106],"These":[107,335],"devices":[108],"unmediated":[111],"direct":[112,149,173,221],"occurring":[115],"interfaces":[117],"made":[118],"up":[119],"immobilized":[123,208,387],"redox-active":[124],"specie,":[125],"current":[128],"transduction":[129],"interaction,":[133],"resulting":[134],"amperometric":[137],"detection":[138],"An":[142],"attractive":[143],"feature":[144],"based":[147,203],"possibility":[154],"modulating":[156,280],"properties":[158,373],"using":[163],"protein":[164],"modification":[165],"genetic":[167],"or":[168,207],"chemical":[169],"engineering":[170],"techniques.":[171],"Efficient":[172],"(DET)":[176],"reactions":[177],"have":[178,262],"been":[179,263],"reported":[180],"restricted":[183],"number":[184],"redox":[186,192,283],"enzymes.":[187],"The":[188,285,310,347,371],"majority":[189],"these":[191,289],"enzymes":[193,216],"contain":[194],"metal":[195],"sites":[196],"particularly":[198],"heme.":[199],"Nevertheless,":[200],"several":[201],"studies":[202],"heme-proteins":[205],"adsorbed":[206],"various":[210],"electrodes":[211],"showed":[212],"high-molecular":[214],"weight":[215,240],"not":[218],"amenable":[219],"electrical":[222],"communication":[223],"electrode.":[226],"Our":[227],"strategy":[228],"overcome":[230],"above":[232],"limitations":[233],"exploitation":[236],"artificial":[238,337],"low-molecular":[239],"proteins,":[241],"designed":[242],"rational":[244],"bases,":[245],"possess":[247],"required":[249],"activity.":[250],"In":[251],"our":[252],"laboratory,":[253],"new":[255],"class":[256,319],"heme-peptide":[258],"conjugate,":[259],"named":[260],"Mimochromes,":[261,321],"developed,":[264],"aim":[267,348],"understanding":[269],"effects":[271],"peptide":[274,303],"chain":[275],"composition":[276],"conformation":[278,300],"heme":[282],"potential.":[284],"main":[286],"features":[287],"molecules":[290,338],"covalent":[293],"structure":[294],"well":[297],"defined":[298],"helical":[299],"chains":[304],"linked":[305],"deuteroporphyrin":[308],"ring.":[309],"presented":[311],"work":[312],"exploits":[313],"use":[315],"two":[317,336,376],"different":[318],"penta-coordinated":[323],"Fe(III)-Mimochrome":[324,329],"VI":[325],"esa-coordinated":[328],"IV":[330],"Lys3,":[331],"technology.":[334],"were":[339,378,385],"used":[340],"bio-recognition":[342],"elements/signal":[343],"transducers":[344],"biosensing.":[346],"this":[350],"thesis":[351],"was":[352,403],"develop":[354],"optimise":[356],"catalytic":[357],"affinity-based":[359],"biosensors":[360,396],"rapid":[363],"screening":[364],"target":[366],"analytes":[367],"complex":[369],"matrices.":[370],"Mimochromes":[377],"studied":[379],"voltammetric":[381],"techniques,":[382],"then":[383],"they":[384],"suitably":[386],"gold":[390],"surface":[392],"achieve":[394],"construction":[397],"finally":[399],"analyte":[401],"performed.":[404]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}