{"id":"https://openalex.org/W1536024176","doi":"https://doi.org/10.1016/s0021-9258(19)39663-2","title":"The E2 in equilibrium E1 transition of the Ca2(+)-ATPase from plasma membranes studied by phosphorylation.","display_name":"The E2 in equilibrium E1 transition of the Ca2(+)-ATPase from plasma membranes studied by phosphorylation.","publication_year":1990,"publication_date":"1990-03-01","ids":{"openalex":"https://openalex.org/W1536024176","doi":"https://doi.org/10.1016/s0021-9258(19)39663-2","mag":"1536024176","pmid":"https://pubmed.ncbi.nlm.nih.gov/2137459"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)39663-2","doi":"https://doi.org/10.1016/s0021-9258(19)39663-2","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)39663-2","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)39663-2","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045928290","display_name":"Hugo Pedro Adamo","orcid":"https://orcid.org/0000-0002-1712-8049","observed_orcids":["https://orcid.org/0000-0002-1712-8049"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I24354313","display_name":"Universidad de Buenos Aires","ror":"https://ror.org/0081fs513","country_code":"AR","type":"education","lineage":["https://openalex.org/I24354313"]},{"id":"https://openalex.org/I4210149401","display_name":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas","ror":"https://ror.org/0469ejq24","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4210149401","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"H P Adamo","raw_affiliation_strings":["Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (Universidad de Buenos Aires-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas), Facultad de Farmacia y Bioqu\u00edmica, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (Universidad de Buenos Aires-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas), Facultad de Farmacia y Bioqu\u00edmica, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I24354313","https://openalex.org/I4210149401"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079742276","display_name":"Alcides F. Rega","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I24354313","display_name":"Universidad de Buenos Aires","ror":"https://ror.org/0081fs513","country_code":"AR","type":"education","lineage":["https://openalex.org/I24354313"]},{"id":"https://openalex.org/I4210149401","display_name":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas","ror":"https://ror.org/0469ejq24","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4210149401","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"A F Rega","raw_affiliation_strings":["Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (Universidad de Buenos Aires-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas), Facultad de Farmacia y Bioqu\u00edmica, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (Universidad de Buenos Aires-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas), Facultad de Farmacia y Bioqu\u00edmica, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I24354313","https://openalex.org/I4210149401"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108189438","display_name":"P. J. Garrahan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I24354313","display_name":"Universidad de Buenos Aires","ror":"https://ror.org/0081fs513","country_code":"AR","type":"education","lineage":["https://openalex.org/I24354313"]},{"id":"https://openalex.org/I4210149401","display_name":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas","ror":"https://ror.org/0469ejq24","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4210149401","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"P J Garrahan","raw_affiliation_strings":["Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (Universidad de Buenos Aires-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas), Facultad de Farmacia y Bioqu\u00edmica, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Qu\u00edmica y Fisicoqu\u00edmica Biol\u00f3gicas (Universidad de Buenos Aires-Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas), Facultad de Farmacia y Bioqu\u00edmica, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I24354313","https://openalex.org/I4210149401"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.9568,"has_fulltext":false,"cited_by_count":35,"citation_normalized_percentile":{"value":0.71092383,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"265","issue":"7","first_page":"3789","last_page":"3792"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":0.9639000296592712,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":0.9639000296592712,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.022099999710917473,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ca2-atpase","display_name":"Ca2+-ATPase","score":0.9660000205039978},{"id":"https://openalex.org/keywords/plasma-membrane","display_name":"plasma membrane","score":0.6520000100135803},{"id":"https://openalex.org/keywords/phosphorylation","display_name":"phosphorylation","score":0.6510000228881836},{"id":"https://openalex.org/keywords/calcium-binding","display_name":"calcium binding","score":0.46399998664855957},{"id":"https://openalex.org/keywords/magnesium-activation","display_name":"magnesium activation","score":0.6039999723434448},{"id":"https://openalex.org/keywords/trypsin-proteolysis","display_name":"trypsin proteolysis","score":0.8479999899864197},{"id":"https://openalex.org/keywords/calmodulin","display_name":"calmodulin","score":0.5809999704360962}],"concepts":[{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.8069971799850464},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.6992794275283813},{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.692566454410553},{"id":"https://openalex.org/C82706917","wikidata":"https://www.wikidata.org/wiki/Q10251","display_name":"Plasma","level":2,"score":0.6099607944488525},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5704827308654785},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5197067856788635},{"id":"https://openalex.org/C194232998","wikidata":"https://www.wikidata.org/wiki/Q1606712","display_name":"Transition (genetics)","level":3,"score":0.5179611444473267},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3737459182739258},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3242030143737793},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.24530848860740662},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24087834358215332},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.10159403085708618},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000252","descriptor_name":"Calcium-Transporting ATPases","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004910","descriptor_name":"Erythrocyte Membrane","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008274","descriptor_name":"Magnesium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008433","descriptor_name":"Mathematics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)39663-2","doi":"https://doi.org/10.1016/s0021-9258(19)39663-2","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)39663-2","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2137459","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2137459","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)39663-2","doi":"https://doi.org/10.1016/s0021-9258(19)39663-2","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)39663-2","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W88911135","https://openalex.org/W611620210","https://openalex.org/W1238743721","https://openalex.org/W1493463750","https://openalex.org/W1506884783","https://openalex.org/W1513298671","https://openalex.org/W1531215851","https://openalex.org/W1559192320","https://openalex.org/W1602093697","https://openalex.org/W1799352521","https://openalex.org/W1975402293","https://openalex.org/W1981126733","https://openalex.org/W2029978381","https://openalex.org/W2341262339"],"related_works":["https://openalex.org/W1981126733","https://openalex.org/W1977740532","https://openalex.org/W1531215851","https://openalex.org/W1983028318","https://openalex.org/W75962083","https://openalex.org/W2030270831","https://openalex.org/W2028116867","https://openalex.org/W2229040683","https://openalex.org/W1997605108","https://openalex.org/W1515461142"],"abstract_inverted_index":{"The":[0,76,94],"relative":[1],"abundance":[2],"of":[3,10,13,19,28,30,58,62,78,96,109,137,197],"the":[4,11,17,25,31,46,56,63,67,72,91,107,125,135,158,176,195,208,215],"two":[5],"conformers":[6],"(E1":[7],"and":[8,16,174,193],"E2)":[9],"Ca2(+)-ATPase":[12,32,64,216],"plasma":[14],"membranes":[15,37,166],"rates":[18],"their":[20],"interconversion":[21],"were":[22,187,223],"estimated":[23],"measuring":[24],"initial":[26],"velocity":[27],"phosphorylation":[29,52,219],"in":[33,66,156,164,194],"pig":[34],"red":[35],"cell":[36],"at":[38,90,147,167,189],"37":[39,168],"degrees":[40,169],"C.":[41],"This":[42],"was":[43,65,83,100,113,142,180],"based":[44],"on":[45],"hypothesis":[47],"that":[48,87,163,175,204],"only":[49],"E1":[50,99,110,173],"catalyzes":[51],"from":[53],"ATP.":[54],"In":[55,134],"absence":[57,196],"ligands":[59,199],"near":[60,190],"90%":[61],"E2":[68,97,112],"conformation.":[69],"Ca2+":[70,79,140,153,171],"shifted":[71],"equilibrium":[73],"toward":[74],"E1.":[75,224],"K0.5":[77],"for":[80],"this":[81,148],"effect":[82],"15":[84],"microM,":[85],"suggesting":[86],"it":[88,222],"acted":[89],"transport":[92],"site.":[93],"conversion":[95,108],"into":[98,111],"slow":[101],"(t1/2":[102,115],"=":[103],"911":[104],"s)":[105],"while":[106],"faster":[114],"less":[116],"than":[117],"or":[118,202],"equal":[119],"to":[120,131,150],"60":[121],"s).":[122],"Mg2+":[123,155],"accelerated":[124,182],"E2----E1":[126,159,178],"reaction":[127],"lowering":[128],"its":[129],"t1/2":[130,141],"0.25":[132],"s.":[133],"presence":[136],"4":[138],"mM":[139],"7.8":[143],"s,":[144],"as":[145,220],"if":[146,221],"concentration":[149],"some":[151],"extent":[152],"replaced":[154],"accelerating":[157],"reaction.":[160,209],"Results":[161],"suggest":[162],"intact":[165],"C":[170],"stabilized":[172],"Ca2(+)-induced":[177],"transition":[179],"strongly":[181],"by":[183],"Mg2+.":[184],"Both":[185],"cations":[186],"effective":[188],"physiological":[191],"concentrations":[192],"other":[198],"like":[200],"ATP":[201],"calmodulin":[203],"could":[205],"also":[206],"modify":[207],"After":[210],"partial":[211],"proteolysis":[212],"with":[213],"trypsin":[214],"behaved":[217],"during":[218]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}