{"id":"https://openalex.org/W1528295787","doi":"https://doi.org/10.1248/cpb.28.641","title":"A novel method for the fluorometric assay of proteins using hypochlorite-thiamine reagent.","display_name":"A novel method for the fluorometric assay of proteins using hypochlorite-thiamine reagent.","publication_year":1980,"publication_date":"1980-01-01","ids":{"openalex":"https://openalex.org/W1528295787","doi":"https://doi.org/10.1248/cpb.28.641","mag":"1528295787"},"language":"en","primary_location":{"id":"doi:10.1248/cpb.28.641","doi":"https://doi.org/10.1248/cpb.28.641","is_oa":true,"landing_page_url":"https://doi.org/10.1248/cpb.28.641","pdf_url":"https://www.jstage.jst.go.jp/article/cpb1958/28/2/28_2_641/_pdf","source":{"id":"https://openalex.org/S3861430","display_name":"Chemical and Pharmaceutical Bulletin","issn_l":"0009-2363","issn":["0009-2363","1347-5223"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313382","host_organization_name":"Pharmaceutical Society of Japan","host_organization_lineage":["https://openalex.org/P4310313382"],"host_organization_lineage_names":["Pharmaceutical Society of Japan"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical and Pharmaceutical Bulletin","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jstage.jst.go.jp/article/cpb1958/28/2/28_2_641/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103477452","display_name":"Toshio Kinoshita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119182057","display_name":"SHOWA Medical University","ror":"https://ror.org/04mzk4q39","country_code":"JP","type":"education","lineage":["https://openalex.org/I119182057"]},{"id":"https://openalex.org/I4405264840","display_name":"YUKI Gosei Kogyo Co., Ltd. (Japan)","ror":"https://ror.org/034eyr523","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405264840"]},{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"TOSHIO KINOSHITA","raw_affiliation_strings":["School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd","institution_ids":["https://openalex.org/I119182057","https://openalex.org/I4405264840","https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018719486","display_name":"Jun\u2010Ichiro Murayama","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119182057","display_name":"SHOWA Medical University","ror":"https://ror.org/04mzk4q39","country_code":"JP","type":"education","lineage":["https://openalex.org/I119182057"]},{"id":"https://openalex.org/I4405264840","display_name":"YUKI Gosei Kogyo Co., Ltd. (Japan)","ror":"https://ror.org/034eyr523","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405264840"]},{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"JUNICHIRO MURAYAMA","raw_affiliation_strings":["School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd","institution_ids":["https://openalex.org/I119182057","https://openalex.org/I4405264840","https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079061980","display_name":"Kazuyo Murayama","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119182057","display_name":"SHOWA Medical University","ror":"https://ror.org/04mzk4q39","country_code":"JP","type":"education","lineage":["https://openalex.org/I119182057"]},{"id":"https://openalex.org/I4405264840","display_name":"YUKI Gosei Kogyo Co., Ltd. (Japan)","ror":"https://ror.org/034eyr523","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405264840"]},{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"KAZUYO MURAYAMA","raw_affiliation_strings":["School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd","institution_ids":["https://openalex.org/I119182057","https://openalex.org/I4405264840","https://openalex.org/I64189623"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072404837","display_name":"Akio Tsuji","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119182057","display_name":"SHOWA Medical University","ror":"https://ror.org/04mzk4q39","country_code":"JP","type":"education","lineage":["https://openalex.org/I119182057"]},{"id":"https://openalex.org/I4405264840","display_name":"YUKI Gosei Kogyo Co., Ltd. (Japan)","ror":"https://ror.org/034eyr523","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405264840"]},{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"AKIO TSUJI","raw_affiliation_strings":["School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmaceutical Sciences, Kitasato University, School of Pharmaceutical Sciences, Showa University, School of Pharmaceutical Sciences, Showa University, Yuki gosei Kogyo Company Ltd","institution_ids":["https://openalex.org/I119182057","https://openalex.org/I4405264840","https://openalex.org/I64189623"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.7166,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.60109027,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"28","issue":"2","first_page":"641","last_page":"644"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.6083999872207642,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.6083999872207642,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.2460000067949295,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.028300000354647636,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-quantification","display_name":"protein quantification","score":0.9290000200271606},{"id":"https://openalex.org/keywords/fluorometric-assay","display_name":"fluorometric assay","score":0.984000027179718},{"id":"https://openalex.org/keywords/sodium-hypochlorite","display_name":"sodium hypochlorite","score":0.9100000262260437},{"id":"https://openalex.org/keywords/thiamine","display_name":"thiamine","score":0.9940000176429749},{"id":"https://openalex.org/keywords/thiochrome","display_name":"thiochrome","score":0.8410000205039978},{"id":"https://openalex.org/keywords/fluorescence","display_name":"fluorescence","score":0.34200000762939453}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9532960057258606},{"id":"https://openalex.org/C2776776456","wikidata":"https://www.wikidata.org/wiki/Q407204","display_name":"Sodium hypochlorite","level":2,"score":0.8409407138824463},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.8270313739776611},{"id":"https://openalex.org/C2776832558","wikidata":"https://www.wikidata.org/wiki/Q409049","display_name":"Hypochlorite","level":2,"score":0.7625350952148438},{"id":"https://openalex.org/C2776203871","wikidata":"https://www.wikidata.org/wiki/Q83187","display_name":"Thiamine","level":2,"score":0.7431470155715942},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6253315210342407},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5962545275688171},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5087645649909973},{"id":"https://openalex.org/C160845552","wikidata":"https://www.wikidata.org/wiki/Q247562","display_name":"Bradford protein assay","level":2,"score":0.4833762049674988},{"id":"https://openalex.org/C72673979","wikidata":"https://www.wikidata.org/wiki/Q17003157","display_name":"Bicinchoninic acid assay","level":2,"score":0.4621264636516571},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4200672507286072},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.23641619086265564},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1248/cpb.28.641","doi":"https://doi.org/10.1248/cpb.28.641","is_oa":true,"landing_page_url":"https://doi.org/10.1248/cpb.28.641","pdf_url":"https://www.jstage.jst.go.jp/article/cpb1958/28/2/28_2_641/_pdf","source":{"id":"https://openalex.org/S3861430","display_name":"Chemical and Pharmaceutical Bulletin","issn_l":"0009-2363","issn":["0009-2363","1347-5223"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313382","host_organization_name":"Pharmaceutical Society of Japan","host_organization_lineage":["https://openalex.org/P4310313382"],"host_organization_lineage_names":["Pharmaceutical Society of Japan"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical and Pharmaceutical Bulletin","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1248/cpb.28.641","doi":"https://doi.org/10.1248/cpb.28.641","is_oa":true,"landing_page_url":"https://doi.org/10.1248/cpb.28.641","pdf_url":"https://www.jstage.jst.go.jp/article/cpb1958/28/2/28_2_641/_pdf","source":{"id":"https://openalex.org/S3861430","display_name":"Chemical and Pharmaceutical Bulletin","issn_l":"0009-2363","issn":["0009-2363","1347-5223"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313382","host_organization_name":"Pharmaceutical Society of Japan","host_organization_lineage":["https://openalex.org/P4310313382"],"host_organization_lineage_names":["Pharmaceutical Society of Japan"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical and Pharmaceutical Bulletin","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1528295787.pdf","grobid_xml":"https://content.openalex.org/works/W1528295787.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2007714279","https://openalex.org/W2316953819","https://openalex.org/W1965730396","https://openalex.org/W2047170917","https://openalex.org/W2084793285","https://openalex.org/W2399163579","https://openalex.org/W2089993334","https://openalex.org/W1515510482","https://openalex.org/W2032778400","https://openalex.org/W1569816474"],"abstract_inverted_index":{"A":[0],"fluorometric":[1],"assay":[2],"method":[3,40],"for":[4],"proteins":[5],"based":[6],"on":[7],"the":[8,26],"reaction":[9],"of":[10,18,49,53],"peptide":[11],"groups":[12,17],"is":[13,41,45],"described.":[14],"The":[15,38],"N-H":[16],"peptides":[19],"are":[20,29],"chlorinated":[21],"with":[22,33],"sodium":[23],"hypochlorite,":[24],"and":[25,44],"resulting":[27],"N-chloropeptides":[28],"allowed":[30],"to":[31,47],"react":[32],"thiamine,":[34],"giving":[35],"fluorescent":[36],"thiochrome.":[37],"present":[39],"highly":[42],"sensitive":[43],"applicable":[46],"1-10\u03bcg":[48],"protein.":[50],"Little":[51],"variability":[52],"fluorescence":[54],"intensity":[55],"was":[56],"observed":[57],"among":[58],"proteins.":[59]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}