{"id":"https://openalex.org/W1528198565","doi":"https://doi.org/10.1128/jb.173.1.308-314.1991","title":"Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi","display_name":"Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W1528198565","doi":"https://doi.org/10.1128/jb.173.1.308-314.1991","mag":"1528198565","pmid":"https://pubmed.ncbi.nlm.nih.gov/1987122","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/207188"},"language":"en","primary_location":{"id":"doi:10.1128/jb.173.1.308-314.1991","doi":"https://doi.org/10.1128/jb.173.1.308-314.1991","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.173.1.308-314.1991","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC207188","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004796046","display_name":"Andreas L. Meinke","orcid":"https://orcid.org/0009-0003-7413-2209","observed_orcids":["https://orcid.org/0009-0003-7413-2209"]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"A Meinke","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069254907","display_name":"Curtis Braun","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"C Braun","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029009766","display_name":"Neil R. Gilkes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"N R Gilkes","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111833449","display_name":"Douglas G. Kilburn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"D G Kilburn","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101416108","display_name":"Robert C. Miller","orcid":"https://orcid.org/0000-0002-0211-7515","observed_orcids":["https://orcid.org/0000-0002-0211-7515"]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"R C Miller","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111551784","display_name":"R. A. J. Warren","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"R A Warren","raw_affiliation_strings":["Department of Microbiology, University of British Columbia, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of British Columbia, Vancouver, Canada","institution_ids":["https://openalex.org/I141945490"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I141945490"],"apc_list":null,"apc_paid":null,"fwci":14.271,"has_fulltext":false,"cited_by_count":91,"citation_normalized_percentile":{"value":0.99683401,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"173","issue":"1","first_page":"308","last_page":"314"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.490200012922287,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.490200012922287,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.4560999870300293,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.024299999698996544,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cellulomonas-fimi","display_name":"Cellulomonas fimi","score":0.9900000095367432},{"id":"https://openalex.org/keywords/cellulose-binding-domain","display_name":"cellulose-binding domain","score":0.7490000128746033},{"id":"https://openalex.org/keywords/catalytic-domain","display_name":"catalytic domain","score":0.7039999961853027},{"id":"https://openalex.org/keywords/deletion-mutants","display_name":"deletion mutants","score":0.7519999742507935},{"id":"https://openalex.org/keywords/cellulose-hydrolysis","display_name":"cellulose hydrolysis","score":0.7390000224113464},{"id":"https://openalex.org/keywords/cellobiose","display_name":"cellobiose","score":0.8349999785423279},{"id":"https://openalex.org/keywords/cellotetraose","display_name":"cellotetraose","score":0.9739999771118164},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.8100000023841858},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.675000011920929}],"concepts":[{"id":"https://openalex.org/C141603559","wikidata":"https://www.wikidata.org/wiki/Q410324","display_name":"Cellulase","level":3,"score":0.8714447617530823},{"id":"https://openalex.org/C2780412358","wikidata":"https://www.wikidata.org/wiki/Q409017","display_name":"Cellobiose","level":4,"score":0.666435718536377},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.6421073079109192},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5830967426300049},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5154975056648254},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5112862586975098},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.47155141830444336},{"id":"https://openalex.org/C2779251873","wikidata":"https://www.wikidata.org/wiki/Q80294","display_name":"Cellulose","level":2,"score":0.44997602701187134},{"id":"https://openalex.org/C196032511","wikidata":"https://www.wikidata.org/wiki/Q375795","display_name":"Glycoside hydrolase","level":3,"score":0.41913753747940063},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.28769785165786743}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002480","descriptor_name":"Cellulase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002480","descriptor_name":"Cellulase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002872","descriptor_name":"Chromosome Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003062","descriptor_name":"Codon","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006094","descriptor_name":"Gram-Positive Bacteria","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D006094","descriptor_name":"Gram-Positive Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012689","descriptor_name":"Sequence Homology, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/jb.173.1.308-314.1991","doi":"https://doi.org/10.1128/jb.173.1.308-314.1991","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.173.1.308-314.1991","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:79690","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC207188","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:207188","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/207188","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Bacteriol","raw_type":"Text"},{"id":"pmid:1987122","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1987122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bacteriology","raw_type":"Comparative Study"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:79690","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC207188","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W1497315448","https://openalex.org/W1504946493","https://openalex.org/W1528246310","https://openalex.org/W1540454682","https://openalex.org/W1571324174","https://openalex.org/W1575701171","https://openalex.org/W1792015654","https://openalex.org/W1978821538","https://openalex.org/W1985870590","https://openalex.org/W1987090713","https://openalex.org/W1989089713","https://openalex.org/W1993329721","https://openalex.org/W2015192874","https://openalex.org/W2019410656","https://openalex.org/W2019983674","https://openalex.org/W2020710769","https://openalex.org/W2022592054","https://openalex.org/W2031717316","https://openalex.org/W2039386700","https://openalex.org/W2046769757","https://openalex.org/W2049230219","https://openalex.org/W2062340271","https://openalex.org/W2076297501","https://openalex.org/W2080695378","https://openalex.org/W2081542305","https://openalex.org/W2123030953","https://openalex.org/W2136289073","https://openalex.org/W2144634347","https://openalex.org/W2151424668","https://openalex.org/W2170212225","https://openalex.org/W2171201200","https://openalex.org/W3192167180","https://openalex.org/W4249041736"],"related_works":["https://openalex.org/W1834858723","https://openalex.org/W1903231758","https://openalex.org/W2062263034","https://openalex.org/W1651136857","https://openalex.org/W2322588355","https://openalex.org/W1979244518","https://openalex.org/W2339098513","https://openalex.org/W2070533917","https://openalex.org/W2092340538","https://openalex.org/W1977281551"],"abstract_inverted_index":{"The":[0,36,120,141,194],"nucleotide":[1],"sequence":[2,16,142],"of":[3,17,21,34,56,68,83,95,105,118,143,149,161,190],"the":[4,13,61,92,106,113,185,191],"cenB":[5,107],"gene":[6,108],"was":[7],"determined":[8],"and":[9,29,47,79,100,177,201],"used":[10],"to":[11,91,131,147,159],"deduce":[12],"amino":[14,27,58,70,85,116,144],"acid":[15],"endoglucanase":[18],"B":[19],"(CenB)":[20],"Cellulomonas":[22],"fimi.":[23],"CenB":[24,135,150,168,173,183,198],"comprises":[25],"1,012":[26],"acids":[28,49,59,71,86,117,145],"has":[30,136],"a":[31,53,80,110,137,155,162],"molecular":[32],"weight":[33],"105,905.":[35],"polypeptide":[37,111,122],"is":[38,88,123,151,199,205],"divided":[39],"by":[40,65],"so-called":[41],"linker":[42],"sequences":[43],"rich":[44],"in":[45,169],"proline":[46],"hydroxyamino":[48],"into":[50],"five":[51],"domains:":[52],"catalytic":[54],"domain":[55,82],"607":[57],"at":[60],"N":[62],"terminus,":[63],"followed":[64],"three":[66],"repeats":[67],"98":[69],"each":[72],"which":[73,87,166],"are":[74],"greater":[75],"than":[76],"60%":[77],"identical,":[78,153],"C-terminal":[81,114],"101":[84],"50%":[89],"identical":[90],"cellulose-binding":[93,139],"domains":[94],"C.":[96],"fimi":[97],"cellulases":[98],"Cex":[99],"CenA.":[101],"A":[102],"deletion":[103],"mutant":[104],"encodes":[109],"lacking":[112],"333":[115],"CenB.":[119],"truncated":[121],"catalytically":[124],"active":[125],"and,":[126],"like":[127],"intact":[128],"CenB,":[129],"binds":[130],"cellulose,":[132],"suggesting":[133],"that":[134,160,202],"second":[138],"site.":[140],"1":[146],"461":[148],"35%":[152],"with":[154,188],"further":[156],"15%":[157],"similarity,":[158],"cellulase":[163,170],"from":[164,179],"avocado,":[165],"places":[167],"family":[171],"E.":[172],"releases":[174],"mostly":[175],"cellobiose":[176],"cellotetraose":[178],"cellohexaose.":[180],"Like":[181],"CenA,":[182],"hydrolyzes":[184],"beta-1,4-glucosidic":[186],"bond":[187],"inversion":[189],"anomeric":[192],"configuration.":[193],"pH":[195],"optimum":[196],"for":[197,203],"8.5,":[200],"CenA":[204],"7.5.":[206]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}