{"id":"https://openalex.org/W1526469284","doi":null,"title":"Etablierung eines GFP-basierten Reportergensystems in Staphylokokken","display_name":"Etablierung eines GFP-basierten Reportergensystems in Staphylokokken","publication_year":2006,"publication_date":"2006-01-01","ids":{"openalex":"https://openalex.org/W1526469284","mag":"1526469284"},"language":"de","primary_location":{"id":"mag:1526469284","doi":null,"is_oa":false,"landing_page_url":"https://ediss.sub.uni-hamburg.de/volltexte/2006/2983/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043787041","display_name":"Gefion C. Franke","orcid":"https://orcid.org/0000-0002-1716-3047","observed_orcids":["https://orcid.org/0000-0002-1716-3047"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Gefion Franke","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1716-3047","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5043787041"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1123,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.39499074,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10593","display_name":"Bacterial biofilms and quorum sensing","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10593","display_name":"Bacterial biofilms and quorum sensing","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.019600000232458115,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.00419999985024333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/staphylococcus-epidermidis","display_name":"Staphylococcus epidermidis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/gfp-reporter","display_name":"GFP reporter","score":0.6899999976158142},{"id":"https://openalex.org/keywords/biofilm-formation","display_name":"biofilm formation","score":0.9010000228881836},{"id":"https://openalex.org/keywords/xylose-inducible-promoter","display_name":"xylose-inducible promoter","score":0.7829999923706055},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.7440000176429749},{"id":"https://openalex.org/keywords/shine-dalgarno-sequence","display_name":"Shine-Dalgarno sequence","score":0.6430000066757202},{"id":"https://openalex.org/keywords/translation-efficiency","display_name":"translation efficiency","score":0.8489999771118164},{"id":"https://openalex.org/keywords/sara","display_name":"SARA","score":0.6420000195503235},{"id":"https://openalex.org/keywords/sod-gene","display_name":"sod gene","score":0.49399998784065247},{"id":"https://openalex.org/keywords/in-situ-gene-expression","display_name":"in situ gene expression","score":0.4869999885559082},{"id":"https://openalex.org/keywords/foreign-body-associated-infections","display_name":"foreign body-associated infections","score":0.5799999833106995}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6369374990463257},{"id":"https://openalex.org/C2777949923","wikidata":"https://www.wikidata.org/wiki/Q131915","display_name":"Staphylococcus epidermidis","level":4,"score":0.6150257587432861},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.529982328414917},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2320350706577301},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.15527686476707458},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.14664393663406372},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"mag:1526469284","doi":null,"is_oa":false,"landing_page_url":"https://ediss.sub.uni-hamburg.de/volltexte/2006/2983/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:2321200727","doi":null,"is_oa":false,"landing_page_url":"https://ediss.sub.uni-hamburg.de/volltexte/2006/2983/pdf/Doktorarbeit.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":160,"referenced_works":["https://openalex.org/W119185776","https://openalex.org/W180228974","https://openalex.org/W560448332","https://openalex.org/W1158446594","https://openalex.org/W1484258617","https://openalex.org/W1492380953","https://openalex.org/W1493879424","https://openalex.org/W1502574671","https://openalex.org/W1525417669","https://openalex.org/W1528363068","https://openalex.org/W1540505601","https://openalex.org/W1549916905","https://openalex.org/W1557495622","https://openalex.org/W1562798956","https://openalex.org/W1575943692","https://openalex.org/W1589053113","https://openalex.org/W1594377573","https://openalex.org/W1606628932","https://openalex.org/W1610052743","https://openalex.org/W1693155018","https://openalex.org/W1757629320","https://openalex.org/W1779034727","https://openalex.org/W1831162069","https://openalex.org/W1838399447","https://openalex.org/W1839855594","https://openalex.org/W1872649016","https://openalex.org/W1899761784","https://openalex.org/W1928272017","https://openalex.org/W1929451968","https://openalex.org/W1935619808","https://openalex.org/W1963645619","https://openalex.org/W1966621920","https://openalex.org/W1967381894","https://openalex.org/W1971033382","https://openalex.org/W1976163665","https://openalex.org/W1980073547","https://openalex.org/W1980659704","https://openalex.org/W1981994435","https://openalex.org/W1983918259","https://openalex.org/W1985793014","https://openalex.org/W1990115953","https://openalex.org/W1990222547","https://openalex.org/W1991292439","https://openalex.org/W1993402464","https://openalex.org/W1996254542","https://openalex.org/W1997757551","https://openalex.org/W2008349272","https://openalex.org/W2012186292","https://openalex.org/W2014293907","https://openalex.org/W2014781338","https://openalex.org/W2015509108","https://openalex.org/W2019410656","https://openalex.org/W2023108062","https://openalex.org/W2029048487","https://openalex.org/W2030695915","https://openalex.org/W2032579724","https://openalex.org/W2035478437","https://openalex.org/W2036270217","https://openalex.org/W2036742051","https://openalex.org/W2037524679","https://openalex.org/W2037712628","https://openalex.org/W2037872675","https://openalex.org/W2038756774","https://openalex.org/W2043901080","https://openalex.org/W2045396511","https://openalex.org/W2053173345","https://openalex.org/W2055294982","https://openalex.org/W2055649671","https://openalex.org/W2056768791","https://openalex.org/W2058254034","https://openalex.org/W2058784664","https://openalex.org/W2061104342","https://openalex.org/W2061514792","https://openalex.org/W2062128984","https://openalex.org/W2063576137","https://openalex.org/W2065603287","https://openalex.org/W2065837503","https://openalex.org/W2068294914","https://openalex.org/W2072930287","https://openalex.org/W2073735874","https://openalex.org/W2077835601","https://openalex.org/W2079660764","https://openalex.org/W2079698171","https://openalex.org/W2081184485","https://openalex.org/W2084312697","https://openalex.org/W2085457220","https://openalex.org/W2085797503","https://openalex.org/W2087735873","https://openalex.org/W2087823077","https://openalex.org/W2096274970","https://openalex.org/W2097016165","https://openalex.org/W2097112201","https://openalex.org/W2100092533","https://openalex.org/W2100283500","https://openalex.org/W2103278057","https://openalex.org/W2104617674","https://openalex.org/W2104923518","https://openalex.org/W2105173502","https://openalex.org/W2106503480","https://openalex.org/W2106553411","https://openalex.org/W2107148353","https://openalex.org/W2107277218","https://openalex.org/W2107827960","https://openalex.org/W2109399675","https://openalex.org/W2110177543","https://openalex.org/W2110310164","https://openalex.org/W2110332811","https://openalex.org/W2111154143","https://openalex.org/W2112075565","https://openalex.org/W2112461089","https://openalex.org/W2115135671","https://openalex.org/W2115366678","https://openalex.org/W2117950800","https://openalex.org/W2118021257","https://openalex.org/W2119612945","https://openalex.org/W2120693070","https://openalex.org/W2121321837","https://openalex.org/W2122608498","https://openalex.org/W2122792108","https://openalex.org/W2123487967","https://openalex.org/W2125564947","https://openalex.org/W2127068865","https://openalex.org/W2127326403","https://openalex.org/W2127599536","https://openalex.org/W2127665463","https://openalex.org/W2128635872","https://openalex.org/W2130493239","https://openalex.org/W2131237419","https://openalex.org/W2131916988","https://openalex.org/W2132114933","https://openalex.org/W2132610259","https://openalex.org/W2135736506","https://openalex.org/W2136520316","https://openalex.org/W2139057877","https://openalex.org/W2139187773","https://openalex.org/W2144440398","https://openalex.org/W2145854162","https://openalex.org/W2146853812","https://openalex.org/W2148907847","https://openalex.org/W2150093407","https://openalex.org/W2150110915","https://openalex.org/W2150417163","https://openalex.org/W2151612263","https://openalex.org/W2154068656","https://openalex.org/W2154384223","https://openalex.org/W2155949199","https://openalex.org/W2156798500","https://openalex.org/W2161018171","https://openalex.org/W2161809782","https://openalex.org/W2163391778","https://openalex.org/W2163681376","https://openalex.org/W2166064969","https://openalex.org/W2167189838","https://openalex.org/W2169027034","https://openalex.org/W2171612171","https://openalex.org/W2315332680","https://openalex.org/W2398756509","https://openalex.org/W2412941513","https://openalex.org/W2416396849","https://openalex.org/W4318900629"],"related_works":["https://openalex.org/W2291339873","https://openalex.org/W2018426858","https://openalex.org/W7070144803","https://openalex.org/W2017279502","https://openalex.org/W3139002080","https://openalex.org/W7025070232","https://openalex.org/W2183797219","https://openalex.org/W2801954928","https://openalex.org/W6990797811","https://openalex.org/W2570391006"],"abstract_inverted_index":{"Ausgehend":[0],"von":[1,11,275,333],"seiner":[2,99],"Fahigkeit":[3,347],"zur":[4,42,348,375],"Biofilmbildung":[5,23,349],"stellt":[6,365],"Staphylococcus":[7,394,402,461],"epidermidis":[8,22,116,168,271,296,335,381,395,403,484,526,640],"im":[9,97,111,248],"Rahmen":[10],"fremdkorperassoziierten":[12],"Infektionen":[13],"den":[14,257],"am":[15],"haufigsten":[16],"isolierten":[17],"Erreger":[18],"dar.":[19],"\r\nDie":[20],"S.":[21,84,115,167,270,295,334,380,483,525,639,679],"verlauft":[24],"typischerweise":[25],"in":[26,44,83,87,114,136,166,254,269,294,379,393,436,460,528,554,586,624,638,655,678],"zwei":[27],"Phasen.":[28],"Nach":[29],"der":[30,33,60,69,106,127,159,178,190,197,199,250,259,276,285,321],"primaren":[31],"Bindung":[32],"Bakterien":[34,140],"an":[35,48,598,618,661,671],"die":[36,74,135,164,221,226,253,265,279,289,309,319,339,346],"zu":[37,186,256,359],"besiedelnde":[38],"Oberflache":[39],"kommt":[40],"es":[41,355],"Akkumulation":[43],"einem":[45,187],"mehrlagigen":[46],"Biofilm,":[47],"dessen":[49],"Entstehung":[50,100],"sowohl":[51],"Polysaccharide":[52],"als":[53],"auch":[54],"Proteine":[55],"beteiligt":[56],"sind.":[57],"Die":[58,176],"Expression":[59,165],"beteiligten":[61],"Faktoren":[62],"unterliegt":[63],"dabei":[64],"einer":[65,208,360,369],"komplexen":[66],"Regulation.":[67],"\r\nZiel":[68],"hier":[70],"vorgelegten":[71],"Arbeit":[72],"war":[73],"Etablierung":[75],"eines":[76,93,108,357],"green":[77],"fluorescent":[78],"protein":[79],"(GFP)":[80,455],"basierten":[81],"Reportergensystems":[82],"epidermidis,":[85],"das":[86],"situ":[88,437],"Untersuchungen":[89,316,330],"differentieller":[90,377],"Genexpresssionsmuster":[91],"innerhalb":[92,356],"lebenden":[94],"Biofilms":[95,358],"und":[96,172,229,353],"Verlauf":[98],"ermoglicht.":[101],"\r\nExprimiert":[102],"man":[103],"GFPmut3.1":[104,268,293,366,648],"unter":[105,121,195,367],"Kontrolle":[107],"xyloseinduzierbaren":[109],"Promotors":[110],"Plasmid":[112],"pAS1":[113],"1457,":[117],"so":[118],"lasst":[119],"sich":[120,234,243,304],"induzierten":[122],"Bedingungen":[123],"kein":[124,214],"signifikanter":[125],"Anstieg":[126,189],"Fluoreszenzintensitat":[128,191,266,322],"messen.":[129],"Diese":[130],"Beobachtung":[131],"ist":[132,264],"moglicherweise":[133],"auf":[134,163,225,244,324,345],"gram-positiven,":[137],"G+C":[138],"armen":[139],"haufig":[141],"herabgesetzte":[142],"Translationseffizienz":[143,282],"heterologer":[144],"Gene":[145],"zuruckzufuhren.":[146],"Deshalb":[147],"wurde":[148,220],"zunachst":[149],"durch":[150,278],"Insertion":[151],"verschiedener":[152],"staphylokokkeneigener":[153],"Shine-Dalgarno-Sequenzen":[154],"vor":[155,273],"dem":[156,325],"gfp":[157,532,542,601],"Startcodon":[158],"Einflus":[160,344],"dieser":[161,223],"Region":[162],"1457":[169,297,336,641],"mittels":[170],"quantitativer":[171],"qualitativer":[173],"Fluoreszenzanalyse":[174,261],"untersucht.":[175,231],"Voranstellung":[177],"SD-Sequenz":[179,198,210,280,287,371],"des":[180,202,267,292,327,331,341],"Haemolysin":[181],"delta":[182],"Gens":[183],"(hld)":[184],"fuhrte":[185],"starken":[188],"nach":[192,301],"Induktion,":[193],"wahrend":[194],"Verwendung":[196,284,368],"Superoxiddismutase":[200],"(sod),":[201],"staphylokokkeneigenen":[203],"akzessorischen":[204],"Regulators":[205],"(sarA)":[206],"oder":[207],"Konsensus":[209],"fur":[211],"gram-positive":[212],"Organismen":[213],"ausreichendes":[215],"Fluoreszenzsignal":[216,307],"messbar":[217],"war.":[218],"Zusatzlich":[219],"Auswirkung":[222],"SD-Sequenzen":[224],"gfpmut3.1":[227,466,582,669],"Transkription":[228],"Translation":[230],"Es":[232],"fand":[233],"bei":[235],"allen":[236],"Konstrukten":[237],"eine":[238],"identische":[239],"Transkriptionseffizienz.":[240],"Gleichzeitig":[241],"liesen":[242],"Translationsebene":[245],"deutliche":[246],"Unterschiede":[247],"GFP-Gehalt":[249],"Proben":[251],"ermitteln,":[252],"Analogie":[255],"Ergebnissen":[258],"quantitativen":[260],"standen.":[262],"Infolgedessen":[263],"also":[272,584],"allem":[274],"masgeblich":[277],"beeinflussten":[281],"abhangig.\r\nUnter":[283],"hld":[286,558],"wurden":[288],"biologischen":[290],"Eigenschaften":[291],"charakterisiert.":[298],"Vier":[299],"Stunden":[300],"Induktion":[302],"lies":[303],"erstmals":[305],"ein":[306,372],"detektieren,":[308],"Halbwertszeit":[310],"betrug":[311],"sieben":[312],"Stunden,":[313],"wobei":[314],"Westernblot":[315],"zeigen,":[317,337],"dass":[318,338,354],"Abnahme":[320],"hauptsachlich":[323],"Abbau":[326],"Proteins":[328],"beruht.":[329],"Biofilmphanotyps":[332],"Anwesenheit":[340],"Reporters":[342],"keinen":[343],"diese":[350],"Stamms":[351],"nimmt,":[352],"homogenen":[361],"GFP-Expression":[362],"kommt.":[363],"Somit":[364],"optimierten":[370],"geeignetes":[373],"Reportergensystem":[374],"Untersuchung":[376],"Genexpression":[378],"dar.\r\n":[382],"\nDevelopment":[383],"of":[384,409,431,444,473,493,530,538,556,573,581,607,647],"a":[385,451,474,548],"Green":[386,452],"Fluorescent":[387,453],"Protein":[388,454],"based":[389,456],"reporter":[390,457],"gene":[391,458,676],"system":[392,459,673],"\r\n\r\nBased":[396],"on":[397,577],"its":[398],"biofilm":[399],"forming":[400],"capacity":[401],"is":[404,642],"the":[405,428,445,471,479,498,513,531,535,557,567,608,630],"most":[406],"frequent":[407],"cause":[408],"foreign-body":[410],"related":[411],"infections.":[412],"Several":[413],"specific":[414],"factors":[415,433],"mediating":[416],"primary":[417],"attachment":[418],"and":[419,477,579,589],"accumulation":[420],"have":[421],"been":[422],"characterized.":[423],"In":[424,594,612,658],"order":[425],"to":[426,449,497,508,629],"analyze":[427],"concerted":[429],"action":[430],"these":[432],"methods":[434],"for":[435,541,665,674],"expression":[438,646,677],"analysis":[439,616],"are":[440],"demanded.":[441],"The":[442,571,635],"aim":[443],"present":[446],"study":[447],"was":[448,501,545,552,564,583,604],"establish":[450],"epidermidis.":[462,680],"Therefore":[463],"we":[464],"cloned":[465],"(Clontech)":[467],"into":[468,482],"pASI":[469],"under":[470,487],"control":[472,500],"xylose-inducible":[475],"promotor":[476],"introduced":[478],"resulting":[480],"construct":[481],"1457.":[485],"However,":[486],"xylose":[488],"induction":[489],"no":[490,562],"significant":[491],"increase":[492],"fluorescence":[494,550,633],"intensity":[495],"compared":[496],"un-induced":[499],"detected.":[502],"This":[503],"finding":[504],"may":[505],"be":[506],"attributed":[507],"inefficient":[509],"translation":[510,543,580,667],"initiation":[511,544,668],"at":[512],"natural":[514],"Shine-Delgarno":[515],"(SD)":[516],"sequence.":[517,570],"Indeed,":[518],"by":[519],"coupling":[520],"different":[521,574],"SD-sequences":[522],"from":[523],"well-characterized":[524],"genes":[527],"front":[529],"start":[533],"codon":[534],"crucial":[536],"impact":[537],"this":[539,656],"element":[540],"demonstrated:":[546],"whereas":[547],"strong":[549],"signal":[551,563],"obtained":[553],"presence":[555],"SD":[559,569,575,609,663],"sequence,":[560],"almost":[561,599],"detected":[565],"with":[566,652],"sarA":[568],"influence":[572],"sequences":[576],"transcription":[578,588,602],"analyzed":[585],"real-time":[587],"semi-quantitative":[590],"western":[591,614],"blotting":[592],"experiments.":[593],"all":[595],"constructs":[596],"investigated":[597],"identical":[600],"level":[603],"found":[605],"regardless":[606],"sequence":[610,664],"present.":[611],"contrast,":[613],"blot":[615],"using":[617,660],"anti-GFP":[619],"antibody":[620],"revealed":[621],"huge":[622],"differences":[623],"GFP":[625],"amounts":[626],"that":[627],"corresponded":[628],"respective":[631],"quantitative":[632],"signal.":[634],"calculated":[636],"half-.life":[637],"7":[643],"h.":[644],"\r\nImportantly":[645],"did":[649],"not":[650],"interfere":[651],"biofilm-positive":[653],"phenotype":[654],"stain":[657],"conclusion,":[659],"appropriate":[662],"optimal":[666],"offers":[670],"attractive":[672],"monitoring":[675]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}