{"id":"https://openalex.org/W1524251490","doi":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","title":"Purification and biological characterization ofN-acetyl ?-D glucosaminidase fromBufo arenarum spermatozoa","display_name":"Purification and biological characterization ofN-acetyl ?-D glucosaminidase fromBufo arenarum spermatozoa","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W1524251490","doi":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","mag":"1524251490","pmid":"https://pubmed.ncbi.nlm.nih.gov/10984420"},"language":"en","primary_location":{"id":"doi:10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","doi":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","is_oa":true,"landing_page_url":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1098-2795%28200010%2957%3A2%3C194%3A%3AAID-MRD11%3E3.0.CO%3B2-0","source":{"id":"https://openalex.org/S125242520","display_name":"Molecular Reproduction and Development","issn_l":"1040-452X","issn":["1040-452X","1098-2795"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Reproduction and Development","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1098-2795%28200010%2957%3A2%3C194%3A%3AAID-MRD11%3E3.0.CO%3B2-0","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5098190350","display_name":"Mar\ufffda Laura Mart\ufffdnez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Mar\ufffda Laura Mart\ufffdnez","raw_affiliation_strings":["IBR (Conicet). Area Biolog\u00eda, Dpto. Ciencias Biol\u00f3gicas, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, UNR. Suipacha, Rosario, Rep\u00fablica Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBR (Conicet). Area Biolog\u00eda, Dpto. Ciencias Biol\u00f3gicas, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, UNR. Suipacha, Rosario, Rep\u00fablica Argentina","institution_ids":["https://openalex.org/I4141589","https://openalex.org/I4210147873"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023182871","display_name":"Luciano Gast\u00f3n Martelotto","orcid":"https://orcid.org/0000-0002-9625-1183","observed_orcids":["https://orcid.org/0000-0002-9625-1183"]},"institutions":[{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Luciano Martelotto","raw_affiliation_strings":["IBR (Conicet). A\u0301rea Biologi\u0301a, Dpto. Ciencias Biolo\u0301gicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, UNR. Suipacha, Rosario, Repu\u0301blica Argentina","IBR (Conicet). \u00c1rea Biolog\u00eda, Dpto. Ciencias Biol\u00f3gicas, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, UNR. Suipacha, Rosario, Rep\u00fablica Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBR (Conicet). A\u0301rea Biologi\u0301a, Dpto. Ciencias Biolo\u0301gicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, UNR. Suipacha, Rosario, Repu\u0301blica Argentina","institution_ids":["https://openalex.org/I4141589","https://openalex.org/I4210147873"]},{"raw_affiliation_string":"IBR (Conicet). \u00c1rea Biolog\u00eda, Dpto. Ciencias Biol\u00f3gicas, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, UNR. Suipacha, Rosario, Rep\u00fablica Argentina","institution_ids":["https://openalex.org/I4141589","https://openalex.org/I4210147873"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5035149252","display_name":"Marcelo O. Cabada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":true,"raw_author_name":"Marcelo O. Cabada","raw_affiliation_strings":["IBR (Conicet). A\u0301rea Biologi\u0301a, Dpto. Ciencias Biolo\u0301gicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, UNR. Suipacha, Rosario, Repu\u0301blica Argentina","IBR (Conicet). \u00c1rea Biolog\u00eda, Dpto. Ciencias Biol\u00f3gicas, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, UNR. Suipacha, Rosario, Rep\u00fablica Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBR (Conicet). A\u0301rea Biologi\u0301a, Dpto. Ciencias Biolo\u0301gicas, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, UNR. Suipacha, Rosario, Repu\u0301blica Argentina","institution_ids":["https://openalex.org/I4141589","https://openalex.org/I4210147873"]},{"raw_affiliation_string":"IBR (Conicet). \u00c1rea Biolog\u00eda, Dpto. Ciencias Biol\u00f3gicas, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, UNR. Suipacha, Rosario, Rep\u00fablica Argentina","institution_ids":["https://openalex.org/I4141589","https://openalex.org/I4210147873"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5035149252"],"corresponding_institution_ids":["https://openalex.org/I4141589","https://openalex.org/I4210147873"],"apc_list":{"value":4260,"currency":"USD","value_usd":4260},"apc_paid":null,"fwci":0.4838,"has_fulltext":true,"cited_by_count":33,"citation_normalized_percentile":{"value":0.5277912,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"57","issue":"2","first_page":"194","last_page":"203"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.7139000296592712,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.7139000296592712,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.1256999969482422,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.0625,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/n-acetyl-beta-d-glucosaminidase","display_name":"N-acetyl-beta-D-glucosaminidase","score":0.996999979019165},{"id":"https://openalex.org/keywords/bufo-arenarum","display_name":"Bufo arenarum","score":0.9580000042915344},{"id":"https://openalex.org/keywords/spermatozoa","display_name":"spermatozoa","score":0.8980000019073486},{"id":"https://openalex.org/keywords/acrosome","display_name":"acrosome","score":0.6800000071525574},{"id":"https://openalex.org/keywords/vitelline-envelope","display_name":"vitelline envelope","score":0.8740000128746033},{"id":"https://openalex.org/keywords/fertilisation-biology","display_name":"fertilisation (biology)","score":0.8600000143051147},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8370000123977661},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.6859999895095825},{"id":"https://openalex.org/keywords/in-vitro-fertilization","display_name":"in vitro fertilization","score":0.6990000009536743},{"id":"https://openalex.org/keywords/toads","display_name":"toads","score":0.6159999966621399}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7870746850967407},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.661179780960083},{"id":"https://openalex.org/C88972607","wikidata":"https://www.wikidata.org/wiki/Q2666904","display_name":"Human fertilization","level":2,"score":0.6351678371429443},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6298993229866028},{"id":"https://openalex.org/C2777174400","wikidata":"https://www.wikidata.org/wiki/Q6750944","display_name":"Mannosidase","level":3,"score":0.5798010230064392},{"id":"https://openalex.org/C196032511","wikidata":"https://www.wikidata.org/wiki/Q375795","display_name":"Glycoside hydrolase","level":3,"score":0.5574281811714172},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5488645434379578},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.4900246858596802},{"id":"https://openalex.org/C2779995066","wikidata":"https://www.wikidata.org/wiki/Q163261","display_name":"Acrosome reaction","level":3,"score":0.4683339595794678},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.4440039098262787},{"id":"https://openalex.org/C2778252442","wikidata":"https://www.wikidata.org/wiki/Q421111","display_name":"Acrosome","level":3,"score":0.4415168762207031},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4230136275291443},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3771306276321411},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.10601538419723511},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.08035147190093994}],"mesh":[{"descriptor_ui":"D000118","descriptor_name":"Acetylglucosaminidase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D000118","descriptor_name":"Acetylglucosaminidase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000118","descriptor_name":"Acetylglucosaminidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000918","descriptor_name":"Antibody Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002022","descriptor_name":"Bufo arenarum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005306","descriptor_name":"Fertilization","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014817","descriptor_name":"Vitelline Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","doi":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","is_oa":true,"landing_page_url":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1098-2795%28200010%2957%3A2%3C194%3A%3AAID-MRD11%3E3.0.CO%3B2-0","source":{"id":"https://openalex.org/S125242520","display_name":"Molecular Reproduction and Development","issn_l":"1040-452X","issn":["1040-452X","1098-2795"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Reproduction and Development","raw_type":"journal-article"},{"id":"pmid:10984420","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10984420","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular reproduction and development","raw_type":"Journal Article"},{"id":"pmh:oai:ri.conicet.gov.ar:11336/71723","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11336/71723","pdf_url":null,"source":{"id":"https://openalex.org/S4306402455","display_name":"CONICET Digital (CONICET)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210148385","host_organization_name":"Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society","host_organization_lineage":["https://openalex.org/I4210148385"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","doi":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","is_oa":true,"landing_page_url":"https://doi.org/10.1002/1098-2795(200010)57:2<194::aid-mrd11>3.0.co;2-0","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/1098-2795%28200010%2957%3A2%3C194%3A%3AAID-MRD11%3E3.0.CO%3B2-0","source":{"id":"https://openalex.org/S125242520","display_name":"Molecular Reproduction and Development","issn_l":"1040-452X","issn":["1040-452X","1098-2795"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Reproduction and Development","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334832","display_name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","ror":"https://ror.org/03stxzb56"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1524251490.pdf","grobid_xml":"https://content.openalex.org/works/W1524251490.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W74827623","https://openalex.org/W194655167","https://openalex.org/W289395296","https://openalex.org/W1489704976","https://openalex.org/W1491373961","https://openalex.org/W1535201878","https://openalex.org/W1594700207","https://openalex.org/W1684468925","https://openalex.org/W1775749144","https://openalex.org/W1984899115","https://openalex.org/W1985148293","https://openalex.org/W1985266378","https://openalex.org/W1994472645","https://openalex.org/W1994741546","https://openalex.org/W2008791387","https://openalex.org/W2014264952","https://openalex.org/W2016379937","https://openalex.org/W2029065719","https://openalex.org/W2050951352","https://openalex.org/W2051655018","https://openalex.org/W2052865938","https://openalex.org/W2061641331","https://openalex.org/W2063281392","https://openalex.org/W2064568036","https://openalex.org/W2065098763","https://openalex.org/W2079650309","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2103099124","https://openalex.org/W2111301002","https://openalex.org/W2115859769","https://openalex.org/W2124666034","https://openalex.org/W2130578424","https://openalex.org/W2133505442","https://openalex.org/W2144640860","https://openalex.org/W2153416567","https://openalex.org/W2153915253","https://openalex.org/W2158991305","https://openalex.org/W2163582404","https://openalex.org/W2210776648","https://openalex.org/W2332218212","https://openalex.org/W4300835843"],"related_works":["https://openalex.org/W1990847765","https://openalex.org/W2029065719","https://openalex.org/W2051655018","https://openalex.org/W1994741546","https://openalex.org/W2153416567","https://openalex.org/W1986220129","https://openalex.org/W2000198083","https://openalex.org/W2014264952","https://openalex.org/W2133505442","https://openalex.org/W2057167765"],"abstract_inverted_index":{"Fertilization":[0],"in":[1,45,83,89,108,115,178,196,210,225],"Bufo":[2],"arenarum":[3],"requires":[4],"the":[5,9,21,29,32,49,79,99,123,130,141,179,186,192,204,207,211],"sperm":[6,19,51],"to":[7,28,86,136],"penetrate":[8],"egg":[10],"envelopes.":[11],"The":[12,61,96,110],"incubation":[13],"of":[14,98,105,153,165,185,206,213],"isolated":[15],"vitelline":[16,87],"envelopes":[17,88],"with":[18,149,191],"induces":[20],"acrosome":[22],"reaction,":[23],"releasing":[24],"proteases":[25],"and":[26,40,129],"glycosidases":[27],"media.":[30,95],"In":[31,74,139,182],"present":[33],"work":[34],"N-acetyl-beta-D-glucosaminidase,":[35],"beta-D-galactosidase,":[36],"beta-D-glucosidase,":[37],"alpha-D-mannosidase,":[38],"alpha-L-fucosidase,":[39],"alpha-D-glucosidase":[41],"activities":[42],"are":[43],"measured":[44],"spermatozoa.":[46],"N-acetyl-beta-D-glucosaminidase":[47,77,187,220],"is":[48,65,78],"major":[50],"glycosidase":[52,62,81],"activity":[53,166,177,188],"assayed.":[54],"However,":[55],"N-acetyl-beta-D-galactosamine":[56],"show":[57],"competitive":[58],"inhibitory":[59],"effect.":[60],"pH":[63],"optimum":[64],"3.5":[66],"being":[67],"inhibited":[68],"at":[69,167],"pHs":[70],"higher":[71,175],"than":[72],"7.5.":[73],"our":[75],"study,":[76],"only":[80],"that":[82,91,203,219],"vitro":[84,197],"binds":[85],"conditions":[90],"resemble":[92],"natural":[93],"fertilization":[94,198],"isolation":[97],"active":[100],"enzyme":[101,111,132,143,208],"will":[102],"allow":[103],"studies":[104,173],"its":[106],"role":[107,224],"fertilization.":[109,214,227],"has":[112],"been":[113],"purified":[114],"a":[116,146,150,163],"two-step":[117],"procedure.":[118],"After":[119],"native":[120],"gel":[121],"electrophoresis,":[122],"activity-stained":[124],"band":[125,148],"was":[126,133,189,201],"cut":[127],"out":[128],"eluted":[131],"finally":[134],"subjected":[135],"ConA-sepharose":[137],"chromatography.":[138],"SDS-PAGE,":[140],"denatured":[142],"migrates":[144],"as":[145],"single":[147],"molecular":[151],"mass":[152],"45":[154,169],"kDa.":[155,170],"Furthermore,":[156],"analysis":[157],"by":[158],"size-exclusion":[159],"on":[160],"HPLC":[161],"showed":[162,174],"peak":[164],"around":[168],"Preliminary":[171],"localization":[172],"relative":[176],"acrosomal":[180],"content.":[181],"addition,":[183],"10%":[184],"associated":[190],"reacted":[193],"sperm.":[194],"By":[195],"assay,":[199],"it":[200],"observed":[202],"inhibition":[205,212],"results":[209],"This":[215],"last":[216],"study":[217],"shows":[218],"plays":[221],"an":[222],"important":[223],"toad":[226]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
