{"id":"https://openalex.org/W1517629656","doi":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","title":"NADP+\u2010dependent glutamate dehydrogenase in the Antarctic psychrotolerant bacterium Psychrobacter sp. TAD1","display_name":"NADP+\u2010dependent glutamate dehydrogenase in the Antarctic psychrotolerant bacterium Psychrobacter sp. TAD1","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W1517629656","doi":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","mag":"1517629656","pmid":"https://pubmed.ncbi.nlm.nih.gov/10601858"},"language":"en","primary_location":{"id":"doi:10.1046/j.1432-1327.2000.00972.x","doi":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1432-1327.2000.00972.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1432-1327.2000.00972.x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028641138","display_name":"Raffaela Di Fraia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129308","display_name":"Institute of Protein Biochemistry","ror":"https://ror.org/03eqeqg74","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]},{"id":"https://openalex.org/I4210155236","display_name":"National Research Council","ror":"https://ror.org/04zaypm56","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Raffaela Di Fraia","raw_affiliation_strings":["Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy","institution_ids":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026655809","display_name":"Val\u00e9rie Wilquet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I132053463","display_name":"Universit\u00e9 Libre de Bruxelles","ror":"https://ror.org/01r9htc13","country_code":"BE","type":"education","lineage":["https://openalex.org/I132053463"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Valerie Wilquet","raw_affiliation_strings":["Microbiology Department, University of Brussels (ULB), \nBelgium","Research Institute Jean-Marie-Wiame, Brussels, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbiology Department, University of Brussels (ULB), \nBelgium","institution_ids":["https://openalex.org/I132053463"]},{"raw_affiliation_string":"Research Institute Jean-Marie-Wiame, Brussels, Belgium","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014590044","display_name":"Maria Antonietta Ciardiello","orcid":"https://orcid.org/0000-0002-7203-0554","observed_orcids":["https://orcid.org/0000-0002-7203-0554"]},"institutions":[{"id":"https://openalex.org/I4210129308","display_name":"Institute of Protein Biochemistry","ror":"https://ror.org/03eqeqg74","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]},{"id":"https://openalex.org/I4210155236","display_name":"National Research Council","ror":"https://ror.org/04zaypm56","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"M. Antonietta Ciardiello","raw_affiliation_strings":["Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy","institution_ids":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113501995","display_name":"Vito Carratore","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129308","display_name":"Institute of Protein Biochemistry","ror":"https://ror.org/03eqeqg74","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]},{"id":"https://openalex.org/I4210155236","display_name":"National Research Council","ror":"https://ror.org/04zaypm56","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Vito Carratore","raw_affiliation_strings":["Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy","institution_ids":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009385271","display_name":"Antonella Antignani","orcid":"https://orcid.org/0000-0002-4895-6701","observed_orcids":["https://orcid.org/0000-0002-4895-6701"]},"institutions":[{"id":"https://openalex.org/I4210129308","display_name":"Institute of Protein Biochemistry","ror":"https://ror.org/03eqeqg74","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]},{"id":"https://openalex.org/I4210155236","display_name":"National Research Council","ror":"https://ror.org/04zaypm56","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Antonella Antignani","raw_affiliation_strings":["Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy","institution_ids":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050578877","display_name":"Laura Camardella","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129308","display_name":"Institute of Protein Biochemistry","ror":"https://ror.org/03eqeqg74","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]},{"id":"https://openalex.org/I4210155236","display_name":"National Research Council","ror":"https://ror.org/04zaypm56","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Laura Camardella","raw_affiliation_strings":["Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy","institution_ids":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085729827","display_name":"Nicolas Glansdorff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I13469542","display_name":"Vrije Universiteit Brussel","ror":"https://ror.org/006e5kg04","country_code":"BE","type":"education","lineage":["https://openalex.org/I13469542"]},{"id":"https://openalex.org/I2802017950","display_name":"Vlaams Instituut voor Biotechnologie","ror":"https://ror.org/03xrhmk39","country_code":"BE","type":"facility","lineage":["https://openalex.org/I2802017950"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Nicolas Glansdorff","raw_affiliation_strings":["Flanders Interuniversity Institute for Biotechnology (VIIB), Brussels, Belgium","Microbiology Department, University of Brussels (VUB), Belgium","Research Institute Jean-Marie-Wiame, Brussels, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Flanders Interuniversity Institute for Biotechnology (VIIB), Brussels, Belgium","institution_ids":["https://openalex.org/I2802017950"]},{"raw_affiliation_string":"Microbiology Department, University of Brussels (VUB), Belgium","institution_ids":["https://openalex.org/I13469542"]},{"raw_affiliation_string":"Research Institute Jean-Marie-Wiame, Brussels, Belgium","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113859559","display_name":"Guido di Prisco","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129308","display_name":"Institute of Protein Biochemistry","ror":"https://ror.org/03eqeqg74","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]},{"id":"https://openalex.org/I4210155236","display_name":"National Research Council","ror":"https://ror.org/04zaypm56","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Guido di Prisco","raw_affiliation_strings":["Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Biochemistry and Enzymology, CNR, Napoli, Italy","institution_ids":["https://openalex.org/I4210129308","https://openalex.org/I4210155236"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":6.0467,"has_fulltext":true,"cited_by_count":53,"citation_normalized_percentile":{"value":0.9618921,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":100},"biblio":{"volume":"267","issue":"1","first_page":"121","last_page":"131"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12180","display_name":"Polar Research and Ecology","score":0.7128999829292297,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12180","display_name":"Polar Research and Ecology","score":0.7128999829292297,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.17139999568462372,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.029400000348687172,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutamate-dehydrogenase","display_name":"glutamate dehydrogenase","score":0.9549999833106995},{"id":"https://openalex.org/keywords/nadp-dependent-enzyme","display_name":"NADP-dependent enzyme","score":0.8949999809265137},{"id":"https://openalex.org/keywords/psychrotolerant-bacteria","display_name":"psychrotolerant bacteria","score":0.9769999980926514},{"id":"https://openalex.org/keywords/antarctic-bacteria","display_name":"Antarctic bacteria","score":0.925000011920929},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.7419999837875366},{"id":"https://openalex.org/keywords/gene-cloning-and-expression","display_name":"gene cloning and expression","score":0.7990000247955322},{"id":"https://openalex.org/keywords/protein-flexibility","display_name":"protein flexibility","score":0.5879999995231628},{"id":"https://openalex.org/keywords/optimal-temperature","display_name":"optimal temperature","score":0.718999981880188},{"id":"https://openalex.org/keywords/thermal-inactivation","display_name":"thermal inactivation","score":0.8399999737739563},{"id":"https://openalex.org/keywords/positive-cooperativity","display_name":"positive cooperativity","score":0.8569999933242798},{"id":"https://openalex.org/keywords/alpha-ketoglutarate","display_name":"alpha-ketoglutarate","score":0.9739999771118164},{"id":"https://openalex.org/keywords/nadph","display_name":"NADPH","score":0.9620000123977661},{"id":"https://openalex.org/keywords/enzyme-regulation","display_name":"enzyme regulation","score":0.7379999756813049}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6779378652572632},{"id":"https://openalex.org/C2777202666","wikidata":"https://www.wikidata.org/wiki/Q417383","display_name":"Glutamate dehydrogenase","level":4,"score":0.6690301895141602},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6545433402061462},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5684759616851807},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.5252758264541626},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.5251726508140564},{"id":"https://openalex.org/C2779268744","wikidata":"https://www.wikidata.org/wiki/Q407479","display_name":"Oxidoreductase","level":3,"score":0.44972869753837585},{"id":"https://openalex.org/C75520062","wikidata":"https://www.wikidata.org/wiki/Q15321392","display_name":"NAD+ kinase","level":3,"score":0.4356890916824341},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.43123680353164673},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.4135262072086334},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.41321861743927},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.26197153329849243},{"id":"https://openalex.org/C61174792","wikidata":"https://www.wikidata.org/wiki/Q420510","display_name":"Glutamate receptor","level":3,"score":0.13995906710624695},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09602797031402588},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000864","descriptor_name":"Antarctic Regions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003062","descriptor_name":"Codon","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007656","descriptor_name":"Ketoglutaric Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016366","descriptor_name":"Open Reading Frames","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016946","descriptor_name":"Gram-Negative Aerobic Rods and Cocci","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018086","descriptor_name":"Glutamate Dehydrogenase (NADP+)","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018086","descriptor_name":"Glutamate Dehydrogenase (NADP+)","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D018086","descriptor_name":"Glutamate Dehydrogenase (NADP+)","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018086","descriptor_name":"Glutamate Dehydrogenase (NADP+)","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020563","descriptor_name":"Gammaproteobacteria","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D020816","descriptor_name":"Amino Acid Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1432-1327.2000.00972.x","doi":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1432-1327.2000.00972.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:10601858","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10601858","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1046/j.1432-1327.2000.00972.x","doi":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1432-1327.2000.00972.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1432-1327.2000.00972.x","source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320327336","display_name":"Vlaamse regering","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1517629656.pdf","grobid_xml":"https://content.openalex.org/works/W1517629656.grobid-xml"},"referenced_works_count":43,"referenced_works":["https://openalex.org/W80301506","https://openalex.org/W187013985","https://openalex.org/W1544748796","https://openalex.org/W1563999781","https://openalex.org/W1571200168","https://openalex.org/W1588719950","https://openalex.org/W1975304761","https://openalex.org/W1986550844","https://openalex.org/W1989006277","https://openalex.org/W1993066793","https://openalex.org/W1995404325","https://openalex.org/W2004517853","https://openalex.org/W2004674004","https://openalex.org/W2006887615","https://openalex.org/W2011067814","https://openalex.org/W2016504665","https://openalex.org/W2021514815","https://openalex.org/W2036941878","https://openalex.org/W2038533609","https://openalex.org/W2051741683","https://openalex.org/W2059085556","https://openalex.org/W2064614137","https://openalex.org/W2066993118","https://openalex.org/W2068301872","https://openalex.org/W2068372391","https://openalex.org/W2086683540","https://openalex.org/W2089844406","https://openalex.org/W2094965879","https://openalex.org/W2118558198","https://openalex.org/W2123949437","https://openalex.org/W2128635872","https://openalex.org/W2134794101","https://openalex.org/W2135225501","https://openalex.org/W2135769863","https://openalex.org/W2138270253","https://openalex.org/W2151822784","https://openalex.org/W2214447253","https://openalex.org/W2271440286","https://openalex.org/W2312838632","https://openalex.org/W2314225431","https://openalex.org/W2336117697","https://openalex.org/W4238490001","https://openalex.org/W4241658954"],"related_works":["https://openalex.org/W2053807978","https://openalex.org/W2042733629","https://openalex.org/W2751855749","https://openalex.org/W2144770939","https://openalex.org/W1983934365","https://openalex.org/W2044442328","https://openalex.org/W1863070284","https://openalex.org/W2020686153","https://openalex.org/W1614232742","https://openalex.org/W2053005601"],"abstract_inverted_index":{"The":[0,50,56,80,102,120,170],"Antarctic":[1],"psychrotolerant":[2],"bacterium":[3],"Psychrobacter":[4,144],"sp.":[5,145],"TAD1":[6,146],"contains":[7],"two":[8,134],"distinct":[9],"glutamate":[10],"dehydrogenases":[11],"(GDH),":[12],"each":[13],"specific":[14],"for":[15,218,230],"either":[16],"NADP+":[17],"or":[18,182],"NAD+.":[19],"This":[20,204],"feature":[21,205,224],"is":[22,124,151,180,206],"quite":[23],"unusual":[24],"in":[25,117,130,141,173,209,244],"bacteria,":[26],"which":[27],"generally":[28,207],"have":[29],"a":[30,53,70,73,127,131,215],"single":[31],"GDH.":[32,169,189],"NADP+-dependent":[33],"GDH":[34,44,147,193],"has":[35,45,52,111],"been":[36,46],"purified":[37],"to":[38,108,138,164],"homogeneity":[39],"and":[40,48,63,92,97,126,202,241],"the":[41,114,139,174,194,221,226,237],"gene":[42,64],"encoding":[43],"cloned":[47],"expressed.":[49],"enzyme":[51,195],"hexameric":[54,85],"structure.":[55],"amino":[57],"acid":[58],"sequence":[59,81],"determined":[60],"by":[61],"peptide":[62],"analyses":[65],"comprises":[66],"447":[67],"residues,":[68],"yielding":[69],"protein":[71,142],"with":[72,84,87,94],"molecular":[74],"mass":[75],"of":[76,90,166,186,233],"49":[77],"285":[78],"Da.":[79],"shows":[82],"homology":[83],"GDHs,":[86],"identity":[88],"levels":[89],"52%":[91],"49%":[93],"Escherichia":[95],"coli":[96,168,188,192],"Clostridium":[98],"symbiosum":[99],"GDH,":[100,220],"respectively.":[101],"coenzyme-binding":[103],"fingerprint":[104],"motif":[105],"GXGXXG/A":[106],"(common":[107],"all":[109],"GDHs)":[110],"Ser":[112],"at":[113,161],"last":[115],"position":[116],"this":[118,219],"enzyme.":[119],"overall":[121],"hydrophilic":[122],"character":[123],"increased":[125],"five-residue":[128],"insertion":[129],"loop":[132],"between":[133],"alpha-helices":[135],"may":[136],"contribute":[137],"increase":[140],"flexibility.":[143],"apparent":[148],"temperature":[149,175],"optimum":[150],"shifted":[152],"towards":[153,200],"low":[154],"temperatures,":[155],"whereas":[156],"irreversible":[157],"heat":[158],"inactivation":[159],"occurs":[160],"temperatures":[162],"similar":[163,181],"those":[165],"E.":[167,187,191],"catalytic":[171,239],"efficiency":[172,240],"range":[176],"10-30":[177],"degrees":[178],"C":[179],"lower":[183],"than":[184,236],"that":[185],"Unlike":[190],"exhibits":[196],"marked":[197],"positive":[198],"cooperativity":[199],"2-oxoglutarate":[201],"NADPH.":[203],"absent":[208],"prokaryotic":[210],"GDHs.":[211],"These":[212],"observations":[213],"suggest":[214],"regulatory":[216],"role":[217],"most":[222],"crucial":[223],"being":[225],"structural/functional":[227],"properties":[228],"required":[229],"fine":[231],"regulation":[232],"activity,":[234],"rather":[235],"high":[238],"thermolability":[242],"encountered":[243],"several":[245],"cold-active":[246],"enzymes.":[247]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":23},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}