{"id":"https://openalex.org/W1516768024","doi":null,"title":"Docking-dependent regulation of checkpoint kinase chk1 by the growth regulator p21WAF1","display_name":"Docking-dependent regulation of checkpoint kinase chk1 by the growth regulator p21WAF1","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W1516768024","mag":"1516768024"},"language":"en","primary_location":{"id":"pmh:oai:era.ed.ac.uk:1842/4215","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1842/4215","pdf_url":"https://era.ed.ac.uk/bitstreams/93f3fb81-c964-4914-841d-7886ba5edea3/download","source":{"id":"https://openalex.org/S7407055182","display_name":"ERA","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis or Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://era.ed.ac.uk/bitstreams/93f3fb81-c964-4914-841d-7886ba5edea3/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053755134","display_name":"Yew Kwang Toh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Yew Kwang Toh","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5053755134"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9672999978065491,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9672999978065491,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.017000000923871994,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chk1","display_name":"Chk1","score":0.859000027179718},{"id":"https://openalex.org/keywords/p21","display_name":"p21","score":0.9679999947547913},{"id":"https://openalex.org/keywords/protein-docking","display_name":"protein docking","score":0.6840000152587891},{"id":"https://openalex.org/keywords/allosteric-activation","display_name":"allosteric activation","score":0.9340000152587891},{"id":"https://openalex.org/keywords/autoinhibition","display_name":"autoinhibition","score":0.9210000038146973},{"id":"https://openalex.org/keywords/tryptophan-residues","display_name":"tryptophan residues","score":0.7929999828338623},{"id":"https://openalex.org/keywords/atr-phosphorylation","display_name":"ATR phosphorylation","score":0.7590000033378601},{"id":"https://openalex.org/keywords/dna-damage-response","display_name":"DNA damage response","score":0.828000009059906},{"id":"https://openalex.org/keywords/protein-kinase-substrate-specificity","display_name":"kinase substrate specificity","score":0.6110000014305115},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.7059999704360962}],"concepts":[{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.8462460041046143},{"id":"https://openalex.org/C41685203","wikidata":"https://www.wikidata.org/wiki/Q1974042","display_name":"Docking (animal)","level":2,"score":0.5684001445770264},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4960385262966156},{"id":"https://openalex.org/C2910325170","wikidata":"https://www.wikidata.org/wiki/Q14912280","display_name":"Checkpoint Kinase 2","level":5,"score":0.49054116010665894},{"id":"https://openalex.org/C50001416","wikidata":"https://www.wikidata.org/wiki/Q17861750","display_name":"CHEK1","level":5,"score":0.4871620833873749},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.46796661615371704},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.34284350275993347},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33849281072616577},{"id":"https://openalex.org/C105696609","wikidata":"https://www.wikidata.org/wiki/Q1068809","display_name":"Cell cycle checkpoint","level":4,"score":0.2914925217628479},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28311586380004883},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.19962170720100403},{"id":"https://openalex.org/C2910050888","wikidata":"https://www.wikidata.org/wiki/Q4857068","display_name":"Protein-Serine-Threonine Kinases","level":4,"score":0.19108125567436218},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.18443691730499268},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15219780802726746},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.14924126863479614},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.09574645757675171},{"id":"https://openalex.org/C159110408","wikidata":"https://www.wikidata.org/wiki/Q121176","display_name":"Nursing","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:era.ed.ac.uk:1842/4215","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1842/4215","pdf_url":"https://era.ed.ac.uk/bitstreams/93f3fb81-c964-4914-841d-7886ba5edea3/download","source":{"id":"https://openalex.org/S7407055182","display_name":"ERA","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis or Dissertation"},{"id":"mag:1516768024","doi":null,"is_oa":false,"landing_page_url":"https://era.ed.ac.uk/bitstream/1842/4215/1/Toh2009.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:era.ed.ac.uk:1842/4215","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1842/4215","pdf_url":"https://era.ed.ac.uk/bitstreams/93f3fb81-c964-4914-841d-7886ba5edea3/download","source":{"id":"https://openalex.org/S7407055182","display_name":"ERA","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis or Dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1516768024.pdf","grobid_xml":"https://content.openalex.org/works/W1516768024.grobid-xml"},"referenced_works_count":167,"referenced_works":["https://openalex.org/W67616126","https://openalex.org/W1480741751","https://openalex.org/W1488639892","https://openalex.org/W1514081838","https://openalex.org/W1515564094","https://openalex.org/W1520947267","https://openalex.org/W1532281567","https://openalex.org/W1562228716","https://openalex.org/W1608097728","https://openalex.org/W1696139161","https://openalex.org/W1840855988","https://openalex.org/W1853857034","https://openalex.org/W1859231335","https://openalex.org/W1914194999","https://openalex.org/W1964609719","https://openalex.org/W1966373529","https://openalex.org/W1967203779","https://openalex.org/W1967232610","https://openalex.org/W1968291992","https://openalex.org/W1970924670","https://openalex.org/W1971079672","https://openalex.org/W1972650300","https://openalex.org/W1973507224","https://openalex.org/W1973570584","https://openalex.org/W1974190379","https://openalex.org/W1976544913","https://openalex.org/W1977479343","https://openalex.org/W1978141829","https://openalex.org/W1978664410","https://openalex.org/W1981939799","https://openalex.org/W1982893698","https://openalex.org/W1988280121","https://openalex.org/W1989142029","https://openalex.org/W1992844265","https://openalex.org/W1992945955","https://openalex.org/W1993307543","https://openalex.org/W1994224457","https://openalex.org/W1995753459","https://openalex.org/W2003842525","https://openalex.org/W2003892963","https://openalex.org/W2007042398","https://openalex.org/W2012877669","https://openalex.org/W2013491063","https://openalex.org/W2013860287","https://openalex.org/W2014156638","https://openalex.org/W2015061258","https://openalex.org/W2015519364","https://openalex.org/W2017012572","https://openalex.org/W2019392060","https://openalex.org/W2020347983","https://openalex.org/W2021491682","https://openalex.org/W2022811243","https://openalex.org/W2023961264","https://openalex.org/W2024799052","https://openalex.org/W2027931017","https://openalex.org/W2028044172","https://openalex.org/W2028361915","https://openalex.org/W2029304297","https://openalex.org/W2029934390","https://openalex.org/W2031487445","https://openalex.org/W2031579743","https://openalex.org/W2031913409","https://openalex.org/W2035258932","https://openalex.org/W2036575025","https://openalex.org/W2040115433","https://openalex.org/W2040235495","https://openalex.org/W2043458530","https://openalex.org/W2044958420","https://openalex.org/W2045164156","https://openalex.org/W2045305010","https://openalex.org/W2049531015","https://openalex.org/W2050981751","https://openalex.org/W2051188058","https://openalex.org/W2052553543","https://openalex.org/W2052677994","https://openalex.org/W2055171335","https://openalex.org/W2055222724","https://openalex.org/W2056029006","https://openalex.org/W2056092711","https://openalex.org/W2056771267","https://openalex.org/W2058073881","https://openalex.org/W2061227850","https://openalex.org/W2061562811","https://openalex.org/W2062541978","https://openalex.org/W2062780666","https://openalex.org/W2065588235","https://openalex.org/W2066541818","https://openalex.org/W2071088216","https://openalex.org/W2072088194","https://openalex.org/W2074455942","https://openalex.org/W2074615071","https://openalex.org/W2075190476","https://openalex.org/W2076575466","https://openalex.org/W2076786521","https://openalex.org/W2077569993","https://openalex.org/W2078690152","https://openalex.org/W2080144028","https://openalex.org/W2080572933","https://openalex.org/W2081746026","https://openalex.org/W2081995373","https://openalex.org/W2084089807","https://openalex.org/W2085305642","https://openalex.org/W2085833148","https://openalex.org/W2087351997","https://openalex.org/W2087476908","https://openalex.org/W2088454593","https://openalex.org/W2088529761","https://openalex.org/W2089083932","https://openalex.org/W2089218510","https://openalex.org/W2089277080","https://openalex.org/W2090563156","https://openalex.org/W2090972634","https://openalex.org/W2091851050","https://openalex.org/W2092904031","https://openalex.org/W2095675477","https://openalex.org/W2098624919","https://openalex.org/W2100008990","https://openalex.org/W2101103624","https://openalex.org/W2102233623","https://openalex.org/W2102740981","https://openalex.org/W2102786472","https://openalex.org/W2105513499","https://openalex.org/W2106622937","https://openalex.org/W2109503445","https://openalex.org/W2112190173","https://openalex.org/W2115655831","https://openalex.org/W2115885986","https://openalex.org/W2116537286","https://openalex.org/W2117629868","https://openalex.org/W2118827241","https://openalex.org/W2123408027","https://openalex.org/W2123455365","https://openalex.org/W2128252882","https://openalex.org/W2128680272","https://openalex.org/W2128994729","https://openalex.org/W2129258256","https://openalex.org/W2130200459","https://openalex.org/W2134435289","https://openalex.org/W2136277289","https://openalex.org/W2136910126","https://openalex.org/W2137108719","https://openalex.org/W2138366746","https://openalex.org/W2145647042","https://openalex.org/W2148607448","https://openalex.org/W2151180466","https://openalex.org/W2152440925","https://openalex.org/W2152441728","https://openalex.org/W2154052847","https://openalex.org/W2154141161","https://openalex.org/W2156541342","https://openalex.org/W2157438849","https://openalex.org/W2157905883","https://openalex.org/W2158110021","https://openalex.org/W2158648773","https://openalex.org/W2162369786","https://openalex.org/W2163680039","https://openalex.org/W2166529745","https://openalex.org/W2166642585","https://openalex.org/W2167196101","https://openalex.org/W2171131568","https://openalex.org/W2173315781","https://openalex.org/W2316337840","https://openalex.org/W2328373594","https://openalex.org/W2330879598","https://openalex.org/W2411309046","https://openalex.org/W2411660514","https://openalex.org/W3024398090"],"related_works":["https://openalex.org/W2008477400","https://openalex.org/W2086038348","https://openalex.org/W1969431807","https://openalex.org/W2112190173","https://openalex.org/W2034570201","https://openalex.org/W2111403239","https://openalex.org/W2023860520","https://openalex.org/W2042883687","https://openalex.org/W1984537002","https://openalex.org/W4242513610"],"abstract_inverted_index":{"Checkpoint":[0],"kinase":[1,175],"1":[2],"(Chk1)":[3],"is":[4,30,87,133,247,293,315],"a":[5,106,123],"key":[6],"player":[7],"in":[8,121,226,277],"the":[9,16,40,44,49,55,79,113,126,140,152,157,164,170,174,186,192,217,232,265,282,286,300,307],"DNA":[10],"damage":[11],"response":[12],"signalling":[13],"pathway":[14],"and":[15,28,83,100,202,228,234,309],"mode":[17],"of":[18,58,78,93,139,156,166,180,214,271,285,304],"Chk1":[19,66,99,120,225,242,272,297,319],"activation":[20],"whereby":[21],"it":[22,198],"undergoes":[23],"ATRdependent":[24],"phosphorylation":[25,38,92,305],"at":[26,39,95,306],"Ser317":[27,308],"Ser345":[29,310],"well":[31],"characterised.":[32],"It":[33],"has":[34],"been":[35],"suggested":[36],"that":[37,65,241,264],"ATR":[41,141],"sites":[42,143],"relieves":[43],"auto-inhibitory":[45,301],"action":[46],"conferred":[47],"by":[48,72,109,244,298],"C-terminal":[50,127,267],"negative":[51],"regulatory":[52],"domain":[53,86,117,160,176],"on":[54,173,211],"catalytic":[56,288],"core":[57,287],"Chk1.":[59,260],"In":[60,97],"this":[61,84,212],"study,":[62],"we":[63],"show":[64],"activity":[67],"can":[68,118],"also":[69],"be":[70],"stimulated":[71,243],"docking":[73,85,116,147,159,219],"to":[74,104,135,169,190,200,204,223,249,291,295,317],"an":[75,274],"N-terminal":[76,233],"region":[77,172,188,213,284],"growth":[80],"regulator":[81],"p21waf1":[82],"necessary":[88],"for":[89],"efficient":[90],"Chk1-dependent":[91],"p21":[94,101,115,158,218,292],"Ser146.":[96],"addition,":[98],"are":[102,131,221,255],"shown":[103],"form":[105],"transient":[107],"interaction":[108,230,280,302],"immunoprecipitation.":[110],"Interestingly,":[111,237],"although":[112],"isolated":[114],"activate":[119,224,296],"trans,":[122],"mutant":[124],"where":[125],"70":[128,268],"amino":[129,153,269],"acids":[130,270],"truncated":[132],"refractory":[134,203],"stimulation":[136],"whereas":[137],"mutation":[138],"phosphoacceptor":[142],"does":[144],"not":[145,256],"affect":[146],"dependent":[148],"activation.":[149],"Furthermore,":[150,207,312],"when":[151],"acid":[154],"sequence":[155,165],"was":[161,177],"aligned":[162],"with":[163,258,281],"Chk1,":[167,215],"homology":[168,187],"F":[171,283],"identified.":[178],"Mutation":[179],"two":[181],"conserved":[182],"tryptophan":[183],"residues":[184],"within":[185],"appears":[189],"release":[191],"C-terminus":[193],"from":[194],"intramolecular":[195],"interactions":[196],"rendering":[197],"susceptible":[199],"cleavage":[201],"allosteric":[205],"stimulation.":[206],"small":[208],"peptides":[209],"based":[210],"like":[216],"domain,":[220],"able":[222,248,294,316],"trans":[227],"disrupt":[229],"between":[231],"Cterminal":[235],"domains.":[236],"peptide":[238,246,252,314],"microarray":[239],"showed":[240],"activating":[245,313],"phosphorylate":[250],"novel":[251],"substrates":[253],"which":[254],"observed":[257],"unstimulated":[259],"The":[261],"data":[262],"suggest":[263],"last":[266],"play":[273],"important":[275],"role":[276],"auto-inhibition":[278],"through":[279],"domain.":[289],"Binding":[290],"inhibiting":[299],"independent":[303],"sites.":[311],"modulate":[318],"specificity":[320],"towards":[321],"other":[322],"substrates.":[323]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}